Determination of Icariin in Epimedii Herba by High Performance Liquid Chromatography

고속액체크로마토그라피에 의한 음양곽 중 Icariin의 정량

A new method for quantitative determination of icariin in Epimedii Herba by high performance liquid chromatography was established. A reversed-phase system with $a\;{\mu}Bondapak\;C_{18}$ column using methanol in water(15% to 70%, gradient elution) as a mobile phase was developed. Icariin and spinosin as an internal reference were detected at 350 nm and the analysis was successfully carried out within 30 min.