한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제16권3호
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- Pages.226-230
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- 1988
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
면역화학적 방법에 의한 Cellobiohydrolase 정량
Assay of Cellobiohydrolnse by Column Single Immunodiffusion and Enzyme tinted Immunosorbent Assay
초록
Trichoderma viride가 분비하는 cellobiohydrolase를 항원으로 사용하여 이 섬유소 분해효소에만 특이적인 항체를 얻을 수 있었다. 이 항체를 이용하여 column single immunodiffusion 방법에 의해서 cellobiohydrolase의 농도 2-10
Antibody against cellobiohydrolase purified from Trichoderma viride had been obtained by injection to rabbit. The antibody had a high specificity against the cellobiohydroase evidienced by absence of immunological reaction to other isozymes from Trichoderma viride. Assay limit of cellobiohydrolase was 1-10