한국균학회지 (The Korean Journal of Mycology)
- 제14권3호
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- Pages.225-229
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- 1986
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
한국산(韓國産) 고등(高等) 균류(菌類)의 효소(酵素)에 관한 연구(硏究)( II ) - 목재부후균(木材腐朽菌)인 조개껍질버섯의 섬유소 분해효소의 확인 -
Studies on Enzymes of the Higher Fungi of Korea( II ) - Identification of Cellulolytic Enzyme in Lenzites betulina-
- Park, Wan-Hee (Department of Environemental Engineering, Kyonggi Technical Open Unversity) ;
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Kim, Tae-Hee
(College of Pharmacy, Sookmyung Women's University) ;
- Ro, Ihl-Hyeob (College of Pharmacy, Sookmyung Women's University)
- 발행 : 1986.09.30
초록
Lenzites betulina(Fr.)에서 효소 분획을 추출하여 carboxymethyl cellulose에 대한 분해 작용을 실험하였다. carboxymethyl cellulase의 활성도는 1.65 unit/ml였다.
Cellulosic substance which plays an important role in carbon cycle is most abundant in the nature world. Some higher fungi are able to digest cellulose directly to satisfy their carbohydrate requirement. Then, in order to investigate the enzymatic components of Lenzites betulina (Fr.) being wood rot, that fungus was collected in Kwangneung area. The carpophore of the fungus was smashed with cool distilled water, extracted and salted out by ammonium sulfate. And then the precipitate was purified by dialysing with visking tube and dissolved with pH 7.8 ammonia water, and the extract was filtrated. The fraction of filtrate was obtained as light brown powder after lyophilization, and determined cellulolytic activity. Cellulolytic potency of Lenzites betulina (Fr.) was 1. 65 unit/ml. The cellulase of Lenzites betulina (Fr.) was stable at below