Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.220-220
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- 2004
Development of hFSH Transgenic Embryo by Gene Transfected Bovine Fetal Fibroblasts
- Yang, Byoung-Chul (National Livestock Research Institute, RDA) ;
- Kim, Dong-Hoon (National Livestock Research Institute, RD) ;
- Im, Gi-Sun (National Livestock Research Institute, RD) ;
- Park, Hyo-Suk (National Livestock Research Institute, RD) ;
- Kim, Se-Woong (National Livestock Research Institute, RD) ;
- Seo, Jin-Sung (National Livestock Research Institute, RD) ;
- Hwang, In-Sun (National Livestock Research Institute, RD) ;
- Yang, Bo-Suk (National Livestock Research Institute, RD) ;
- Chang, Won-Kyong (National Livestock Research Institute, RDA)
- Published : 2004.06.01
Abstract
The purpose of this study was to development of transgenic cow using the nuclear transfer. To secrete hFSH in urea, the vector was constructed with UPII promoter. The fetal fibroblast cells (KbFF) were constructed from pregnant day 45 male fetus. The hFSH genes were cotransfected with pcDNA3 (neo) vector to KbFF cells by electroporation. (omitted)