Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)

The Korean Society of Animal Reproduction (한국동물번식학회)
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Expression of GFP Gene in Porcine Embryos after ICSI with Different DNA Binding Methods

  • Han, J. H. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Kim, S.W. (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Lee, Y.K. (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Lee, P.Y. (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Park, C.G. (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Lee, S.E. (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Baek, K.N. (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Lee, H.G. (Division of Animal Biotechnology, National Livestock Research Institute, RD) ;
  • Lee, J.Y. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Chang, W.K. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Park, J.K. (Division of Animal Biotechnology, National Livestock Research Institute, RDA)
  • Published : 2004.06.01
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Abstract

Transgenic animals are produced primarily by microinjecting exogenous DNA into the male pronuclei of a zygote. Microinjection method for gene transmitting is successful in mice but not efficient in farm animals, limiting it's general utility such as a large scale facility and labour. Based on our finding that sperm cells bind with exogenous DNA, sperm was used as a vector for producing transgenic animals to introduced green fluorescence protein(GFP) gene. (omitted)

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