Bovine Embryo Sexing by Loop-Mediated Isothermal Amplification (LAMP

  • Kojiya, S. (Eiken Chemical Co., Ltd.) ;
  • Watanabe, K. (Eiken Chemical Co., Ltd.)
  • Published : 2004.06.01

Abstract

In the bovine embryo transfer industry, sexing preimplantation embryos is an important management tool. Several methods for bovine embryo sexing utilizing polymerase chain reaction (PCR) have been developed. However, they were not popularized because the methods requiretechnical skills and expensive instruments, and are time consuming. PCR also has the risk of false positives due to DNA contamination during the electrophoresis. (omitted)

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