Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
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- Pages.86-86
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- 2002
Follow-Up of Exogenous DNA by Sperm-Mediated Gene Transfer Via Liposome
- Jo Hwang-Yun (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Jo Seong-Geun (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Yun Hui-Jun (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Park Mi-Ryeong (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Im Yeo-Jeong (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Park Jong-Ju (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Kim Jin-Hoe (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University)
- Published : 2002.06.01
Abstract
To examine the feasibility of using a sperm vector system for gene transfer, we have investigated the binding and the uptaking of foreign DNA into the sperm nucleus by PCR, in situ hybridization and LSC. We have also examined the transportation of exogenous DNA into oocytes by immunofluorescene via PCR. Sperm cells were incubated with DNA/liposome complexes (1:4 ratio) in fertilization medium with BSA or without BSA. (omitted)