• 한국전문물리치료학회지
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• 제14권2호
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• pp.37-43
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• 2007

Electrical acupuncture has been shown to induce hypoglycemic effect in streptozotocin diabetic rats. The Zusanli acupoints have been widely used in traditional Chinese medicine to relieve symptoms of diabetes mellitus. But, the effects of electrical acupuncture stimulation of different frequencies are still unclear. We designed an experiment to investigate the effect of electrical acupuncture of high (100 Hz) and low (2 Hz) frequencies on Zusanli acupoint for 15 minutes in streptozotocin diabetic rats. They were divided into 4 groups, high frequency electrical acupuncture (HFEA group, n=5), low frequency electrical acupuncture (LFEA group, n=5), without any treatment (control group, n=5) and normal group (n=5). After 2 weeks of treatment, the HFEA and LFEA groups had a significantly decreased glucose concentration in serum in comparison to the control group. Additionally, the HFEA group had a significantly decreased glucose concentration in serum compared with the LFEA group. Both the HFEA and LFEA groups had a significantly decreased Immunoglobulin G concentration in serum compared with control group. The LFEA and control groups had a significantly increased cholesterol concentration in serum compared with the normal group, but the HFEA did not have a significantly increased cholesterol concentration. Thus we suggest that electrical acupuncture stimulation at the Zusanli acupoints is effective on hypoglycemic effect and immune function. Overall, the therapeutic effects of electrical acupuncture at 100 Hz was better than effects at 2 Hz.

• 동의생리병리학회지
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• 제21권3호
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• pp.748-754
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• 2007

Electroacupuncture is the combined treatment of mechanical and electric stimuli on the needle head, on which electricity runs after acupuncturing at the acupuncture points. It is reported that its treatment effect depends on the parameter of stimulus, which is wave of stimulus, frequency, voltage, and duration of electric current. To get the effective treatment, stimulus intensity over threshold is needed. We planned to experiment whether acupuncture effect is affected by sex, age, and weight. After we conducted electric stimulus at Zusanli(ST 36) which is known to increase small intestinal motility in normal rats, we observed the effects and got the following results. Though the increased effects of small intestinal motility of electro-acupuncture at Zusanli(ST 36) did not show the difference of sex, this effect was observed to have a tendency to decrease according to weight increase without distinction of sex. But the effect of electro-acupuncture shown in 4, 5, 7 weeks old without distinction of sex was observed to have a tendency to decrease with the growing of weeks-old in the male rat group. And the significant change without specific tendency was observed in the 4, 5 weeks old female rat group. This result suggested that sex, weight, and age should be considered to get the efficient electro-acupuncture. The more experimental study should be advanced to investigate the correlation between the factors that can affect the acupuncture treatment, and, afterward, the interconnection into the clinical use.

• 대한소아치과학회지
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• 제25권4호
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• pp.788-796
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• 1998

The purpose of this study was to investigate the effect of various electroacupuncture duration induced by acupuncture point-Zusanli ($S_{36}$) electrical stimulation on inhibition of amplitude of digastric electromyogram (dEMG) evoked by noxious electrical stimuli around the mental foramen. intraperitoneal sodium pentobarbital in an initial dose of 50mg/kg and maintenance doses of 4.5mg/kg/h were given through a cannula in the femoral vein using a constant infusion pump. A pair of stimulating electrodes were inserted for noxious stimuli around the mental foramen. An irritant electronic stimuli pulse (0.2 Hz, 0.1 ms duration) was produced with an intensity of about $1.5{\times}2$ times threshold for evoking the dEMG. The anterior belly of the digastric muscle was exposed and a pair of 0.1mm wire electrodes were inserted for dEMG recording. Acupuncture point stimulation on Zusanli (2 Hz, 250 ${\mu}s$, biphasic pulse, 2 V) was delivered by Dental Electronic Anesthesia (3M, U.S.A). For periods of electronic stimulation of 10, 20, and 30min, the amplitudes of dEMG were measured on the oscilloscope and on the monitor connected to the amplifier. The following results were obtained: The dEMG was decreased to 73.4% of that in the control set after 10 min electroacupunture stimulation (Group I); The dEMG was decreased to 77.1% (10min), 54.0.% (20min) of that in the control set after 20minutes of electroacupunture stimulation (Group II). The dEMG was decreased to 73.3% (10min), 61.9% (20min), 76.2% (30min) of that in the control set after 30 min of electroacupunture stimulation (Group III). From these results, it may be that in the electroacupuncture stimulation on the Zusnali resulted in a reduction of amplitude of dEMG and that the most effective electroacupuncture stimulation period was 20min.

• 대한한방내과학회지
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• 제30권1호
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• pp.191-199
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• 2009

목 적 : 위 미주신경 활성 저하와 유문부 기능 장애를 동시에 가지고 있는 기능성 소화불량증 환자에서 전침 및 한약의 복합처치 치료가 이 두가지 면에서 각각 어떤 영향을 미치는지를 살펴보았다. 방 법 : 장음검사상 위 미주신경 활성 저하와 유문부 기능 장애를 동시에 가지고 있는 환자 10명을 대상으로 치료 전후의 장음 지표 변화를 비교하였다. 장음지표는 % of bowel sound (%BS)와 주 주파수(dominant frequency, DF)를 사용하였으며, %BS(6인 경우를 위 미주신경 활성저하 DF ratio(식후/식 전)<1를 유문부 기능장애로 평가하였다. 환자들에게 2주간 족삼리전 침과 체침이 매일 시술되었으며 평위산 전탕액이 하루 3회 투여되었다. 결 과 : 2주간의 치료 후 DF ratio는 $0.93\pm0.06$에서 $1.06\pm0.04$으로 유의성있게 증가했으며, 10명 중 9명의 환자가 DF ratio>1의 정상치로 회복되었다. %BS 역시 $2.97\pm1.17%$에서 4.27%로 증가했지만 통계적 유의성은 없었으며, 3명의 환자에서 %BS가 6이상으로 정상회복되었다. 결 론 : 위 미주신경 활성 저하와 유문부 기능 장애를 동시에 가지고 있는 기능성 소화불량증 환자에 대한 족삼리 전침과 일반 체침, 평위산의 치료는 미주신경 활성 저하보다는 유문부 기능 장애에 더 유의한 효과를 보였다.

• 대한한의학회지
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• 제26권4호
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• pp.98-107
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• 2005

Objectives: Acupuncture treatment has been clinically used for functional recovery in Parkinson's disease. In the present study, we investigated the effect of acupuncture at Zusanli (ST36) on nigrostriatal dopaminergic neuronal cell death in rats. Methods: A Parkinson's disease model was induced by the unilateral injection of 6-hydroxydopamine (6-OHDA) into the striatum. Acupuncture treatment was performed at Zusanli (ST36) and at the hip, as a non-acupoint, once a day for 14 days. Two weeks after 6-0HDA injection, an apomorphine-induced rotational behavior test showed significant rotational asymmetry in rats with Parkinson's disease. Immunostaining for tyrosine hydroxylase demonstrated a dopaminergic neuronal loss in the substantia nigra and dopaminergic fiber loss in the striatum. Results: Acupuncture at the ST36 acupoint significantly inhibited rotational asymmetry in rats with Parkinson's disease, and also protected against 6-OHDA-induced nigrostriatal dopaminergic neuronal loss. These effects of acupuncture were not observed for non-acupoint acupuncture. Conclusions: The present study shows that acupuncture treatment, especially at the ST36 acupoint, can be used as a useful strategy for the treatment of Parkinson's disease.

• Journal of Acupuncture Research
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• 제19권5호
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• pp.176-188
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• 2002

Objective : Sono-acupoint (SA) therapy is a new therapeutic technique that combined with acupuncture points, herbal medicine and ultrasound therapy. This study was carried out to investigate the analgesic and anti-inflammatory effects of sono-acupoint therapy. Methods : We performed the tail-flick test with normal rats to examine the tail-flick latency (TFL), and the Freund's adjuvant-induced arthritis rat model to examine the edema, skin temperature and serum concentration of c-reactive protein and rheumatoid factor (RF). Herbal SA (HSA) treatment was performed at bilateral Zusanli (ST36) with the hanbang-gel made of several selected herbal drugs in Sprague-Dawley rats (male, $250{\pm}30g$). General SA (GSA) treatment was performed at bilateral Zusanli (ST36) with the gel used in ultrasound therapy. In arthritis rat model, Freund's adjuvant (50mg/ml) was injected in dorsal part of right foot, and these treatments were performed after 15 days. Results : TFL was lengthened after SA treatments. Skin temperature and RF concentration that were the evidence of arthritis in rats were decreased by HSA treatment (P < 0.05). Conclusion : These results indicate that HSA has the analgesic and anti-inflammatory effects in rats, and further developments will produce the advance of this new therapeutic skill.

• 동의생리병리학회지
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• 제17권3호
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• pp.672-676
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• 2003

To investigate the effect of acupuncture on Zusanli (ST 36) in this study, gastrin level of serum by radioimmunoassay was measured at 1, 3, 6 weeks in rats. In other groups, 1 mg/day omeprazole was administered for 1, 3, 6 weeks. Omeprazole increased significantly gastrin level of serum at 1, 3, 6 weeks in a time dependent manner. Acupuncture applied to the ST 36 acupoint decreased at 1, 3, 6 weeks. Furthermore, acupuncture applied to other acupoint, Yanglinquan (GB 34) did not produced significant effect. When the common peroneal nerve was dissected, acupuncture of ST 36 produced the change of gastrin level. These data suggest that acupuncture decreased gastrin level of serum in point specific way and that effect was not related with surrounding nerve.

• The Journal of Korean Physical Therapy
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• 제22권2호
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• pp.47-53
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• 2010

Purpose: This study investigated the effect of electroacupuncture stimulation on changes in blood biochemistry in spinal cord injury (SCI) rats whose cords were damaged by 6-hydroxydopamine (6-OHDA). Methods: Twenty-one Sprague-Dawley adult male rats were assigned to one of three groups: normal (n=7), control (n=7) and experimental group (n=7). The experimental group received electroacupuncture (Es-160, ITO, Japan) for 15 minutes in the form of low frequency (2 Hz) stimulation to the zusanli point. After treatment, we observed motor behavior recovery using the inclined plane test. We also measured serum and CNS concentrations and activities of enzymes including creatine kinase (CK), alkaline phosphatase (ALP), and Immunoglobulin G (IgG). Statistical analysis was done using one-way analysis of variance (ANOVA). Results: Concentrations of CK, ALP and IgG were lower in the experimental group than in the control group. Functional recovery was evaluated by the maximal angle of the inclined board on which rats could maintain their initial position. This allowed us to monitor progressive locomotor recovery. The maximal angles of the inclined plane test were higher in the experimental group than in the control group (p<0.05). Conclusion: The results of this study showed that electroacupuncture to the zusanli point has a therapeutic effect on functional recovery after SCI.

• 대한한방내과학회지
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• 제30권1호
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• pp.1-8
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• 2009

Objective : The aims of this study were to observe how body weight and gastric morphology were changed and whether gastric emptying was impaired in rats with partial pyloric obstruction. and to evaluate whether electroacupuncture was able to restore delayed gastric emptying. Methods : Partial pyloric obstruction was induced by wrapping a nonabsorbable rubber ring around the 1st portion of the duodenum for 2 weeks. Body gain and morphologic changes of stomach were investigated and compared with normal intact rats. Gastric emptying was measured by numbering expelled glass of beads in rats. Rats were divided into 4 groups(non-acupuncture, manual acupuncture. 3Hz-electroacupuncture. 60Hz-electroacupuncture). Stimulus intensity in two electroacupuncture groups was 1.2 times of pain threshold. Results : Partial pyloric obstruction produced a significant loss of body weight and induced a significant increase of gastric surface area. The 60Hz electroacupuncture-stimulated group significantly restored the delayed gastric emptying compared to the other groups of rats with partial pyloric obstruction. Conclusion : 60Hz electroacupuncture stimulation on Zusanli(ST36) showed significant restoration of delayed gastric emptying in rats with partial pyloric obstruction.

• 대한물리의학회지
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• 제5권2호
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• pp.265-272
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• 2010

Purpose : This study was investigated the effect of electroacupuncture stimulation on the change of blood biochemical components in the rat spinal cord injury(SCI) damaged by the 6-hydroxydopamine. Methods : SCI model rats were damaged in L1-L2 injected with 6-hydroxydopamine. The thirty Sprague-Dawley adult male rats were randomly divided into normal group, control group and electroacupuncture group. Experimental groups were applied as electroacupuncture(Es-160, ITO, Japan) for 15minutes during the low frequency(2 Hz) stimulation to zusanli. The enzyme concentration levels analysis of the hematological changes were measured of Glutamate Oxaloacetate Transaminase(GOT), Glutamate Pyruvate Transaminase(GPT), Lactate dehydrogenase(LDH) and motor function recovery change was evaluated by the rota-rod test. Results : This study were as follow : The concentration of GOT, LDH in experimental group was lower than control group(p<.05). The experimental group showed increase of motor function recovery more in compared to control group(p<.05). Conclusion : The results of this study showed that electroacupuncture to zusanli point have an effect on functional recovery after the 6-hydroxydopamine induced SCI in rats.