The Journal of Korean Physical Therapy

The Korean Society of Physical Therapy (대한물리치료학회)
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The Effect of Electroacupuncture on Concentration and Activity of CK, ALP, and IgG in Serum and Central Nervous System of Rats

전침자극이 중추신경계 손상 흰쥐에서 CK, ALP, IgG 효소활성도에 미치는 영향

  • Ha, Mi-Sook (Department of Physical Therapy, College of Masan) ;
  • Park, Rae-Joon (Department of Physical Therapy, College of Rehabilitation Science, Daegu University)
  • 하미숙 (마산대학 물리치료과) ;
  • 박래준 (대구대학교 재활과학대학 물리치료학과)
  • Received : 2010.01.21
  • Accepted : 2010.03.29
  • Published : 2010.04.25
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Abstract

Purpose: This study investigated the effect of electroacupuncture stimulation on changes in blood biochemistry in spinal cord injury (SCI) rats whose cords were damaged by 6-hydroxydopamine (6-OHDA). Methods: Twenty-one Sprague-Dawley adult male rats were assigned to one of three groups: normal (n=7), control (n=7) and experimental group (n=7). The experimental group received electroacupuncture (Es-160, ITO, Japan) for 15 minutes in the form of low frequency (2 Hz) stimulation to the zusanli point. After treatment, we observed motor behavior recovery using the inclined plane test. We also measured serum and CNS concentrations and activities of enzymes including creatine kinase (CK), alkaline phosphatase (ALP), and Immunoglobulin G (IgG). Statistical analysis was done using one-way analysis of variance (ANOVA). Results: Concentrations of CK, ALP and IgG were lower in the experimental group than in the control group. Functional recovery was evaluated by the maximal angle of the inclined board on which rats could maintain their initial position. This allowed us to monitor progressive locomotor recovery. The maximal angles of the inclined plane test were higher in the experimental group than in the control group (p<0.05). Conclusion: The results of this study showed that electroacupuncture to the zusanli point has a therapeutic effect on functional recovery after SCI.

Keywords

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