• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.35-44
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• 2009

This study was carried out to examine the growth performance of 4 species of cool-season grasses and 4 species of zoysiagrasses under salt injury in Seo-san reclaimed area. Grasses were grown on the plots with capillary water interruption layer (WCWIL) and without capillary water interruption layer (WOCWIL) soil systems. Cool-season grass and seeding-type zoysiagrass plots were seeded on 6 Jun, 2006. Vegetative zoysiagrass 'Junggi' was established by sprigging and 'Senock' and 'Millock' were plugged. Electric conductivities of irrigation water (ECw) ranged from 0.28 to $3.3\;dS{\cdot}m^{-1}$. Electric conductivities (ECe) of the soil with capillary water interruption layer and without capillary water interruption layer ranged from 0.55 to $9.4\;dS{\cdot}m^{-1}$ and from 1.84 to $9.4\;dS{\cdot}m^{-1}$ respectively. Leaf color, turf quality, coverage rates, and growth rates were rated visually for 2 years. Zoysiagrass 'Junggi', creeping bentgrass, zoysiagrass 'Senock' and 'Millock' showed acceptable growth at salty fairway condition, while Kentucky bluegrass, perennial ryegrass, Kentucky bluegrass mixed with perennial ryegrass, and seeded zoysiagrass 'Zenith' showed establishment rates below 70%. These results will be useful when choosing turf grass species and cultivars for the golf courses in reclaimed land area.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.222-229
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• 2012

In this study, we attempted to control invaded hetero-species turfgrass in golf courses with herbicides. Asulam sodium SP and trifloxysulfuron-sodium WG (to control creeping bentgrass in zoysiagrass), trifloxysulfuron-sodium WG (to control kentucky bluegrass in zoysiagrass), and mesotrione SC (to control creeping bentgrass in kentucky bluegrass) were selected as efficient herbicides. To creeping bentgrass, asulam sodium SP and trifloxysulfuron-sodium WG until 70 days after application (DAA), mesotrione SC until 230 DAA and to kentucky bluegrass trifloxysulfuron-sodium WG until 110 DAA; the applications showed 90% herbicidal effect. At 4 X recommended application dose, asulam sodium SP, trifloxysulfuronsodium WG (to zoysiagrass) and mesotrione (to kentucky bluegrass) showed insignificant harmful effect. With recommended dose, asulam sodium SP showed 80% control effect of creeping bentgrass in zoysiagrass until 40 DAA and trifloxysulfuron-sodium WG demonstrated 85% control efficiency until 30 DAA. Trifloxysulfuron-sodium WG controlled 85% of kentucky bluegrass in zoysiagrass until 40 DAA. Mesotrione SC with recommended dose showed 95% control efficiency to creeping bentgrass in kentucky bluegrass.

• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.105-116
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• 2011

Transgenic zoysiagrass (Zoysia japonica Steud.) expressing the bar gene inserted in the plant genome has been generated previously through Agrobacterium tumefaciens-mediated transformation. The GM zoysiagrass (event: JG21) permits efficient management of weed control of widely cultivated zoysiagrass fields, reducing the frequency and cost of using various herbicides for weed control. Now we have carried out the environmental risk assessment of JG21 prior to applying to the governmental regulatory agency for the commercial release of the GM turf grass outside of test plots. The morphological phenotypes, molecular analysis, weediness and gene flow from each test plot of JG21 and wild-type zoysiagrasses have been evaluated by selectively analyzing environmental effects. There were no marked differences in morphological phenotypes between JG21 and wild-type grasses. The JG21 retained its stable integration in the host plant in T1 generation, exhibiting a 3:1 segregation ratio according to the Mendelian genetics. We confirmed the copy number (1) of JG21 by using Southern blot analysis, as the transgenic plants were tolerant to ammonium glufosinate throughout the culture period. From cross-fertilization and gene flow studies, we found a 9% cross-pollination rate at the center of JG21 field and 0% at distances over 3 m from the field. The JG21 and wild-type zoysiagrass plants are not considered "weed" because zoysiagrasses generally are not dominant and do not spread into weedy areas easily. We assessed the horizontal gene transfer (HGT) of the transgene DNA to soil microorganisms from JG21 and wild-type plants. The bar gene was not detected from the total genomic DNA extracted from each rhizosphere soil of GM and non-GM Zoysia grass fields. Through the monitoring of JG21 transgene's unintentional release into the environment, we found no evidence for either pollen mediated gene flow of zoysiagrass or seed dispersal from the test field within a 3 km radius of the natural habitat.

• Korean Journal of Weed Science
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• v.31 no.2
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• pp.199-204
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• 2011

This experiment was carried out to investigate the growth responses of zoysiagrass (Zoysia japonica Steud.) as affected by prohexadione-calcium foliar application for the effective labour saving and cost down management in the lawn yard, field and golf course etc. The leaf growth of zoysiagrass treated with prohexadione-calcium one day after mowing was significantly slow compared to the untreated plot. And the effect was continued up to 50 days. The growth inhibition rate at the treated plot was 46 to 50% compared to untreated plot and the leaf widths treated with prohexadione-calcium were narrower than control plot. The change of leaf dry weight 10 days after prohexadione-calcium treated was significantly shown less speedy than untrated plot and the results were continued up to 40 days after application. The SPAD values of the leaf colour were increased in the treated plots and continued for 40 days more.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.1
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• pp.37-44
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• 2007

To investigate the response of proline and ammonia to pathogen infection, plant growth and relevant chemical component were examined in large patch-infected or healthy (control) zoysiagrass during 6 days after treatment. Pathogen-infection increased root mortality by 30% compared to control. Soluble protein was not significantly affected by pathogen-infection except in the leaf at day 6. Ammonia concentration also increased significantly in both leaves and roots of pathogen-infected plants. Proline concentration in leaves and roots increased to 3.4- and 4.5-fold, respectively, compared to those of control at day 6. These results suggest that proline accumulation may be a sensitive biochemical indicator representing the stress intensity caused by pathogen infection in zoysiagrass.

• Weed & Turfgrass Science
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• v.7 no.1
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• pp.54-61
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• 2018

Zoysiagrass (Zoysia spp.) exists spotly in Indonesia and it has potential to be used in parks, golf courses, and football fields. Many football fields and golf course fairways use sand as top soil over native soil. This study aims to analyze growth and quality of two native zoysiagrasses Zis and Zim. Zis is a native zoysiagrass collected in sea-side and Zim is a native zoysiagrass collected in mountain area. Both types of zoysiagrasses were planted at field with altitude of 300 m with various growing media mixes of sand and reservoir's sediment. Thickness of the growing medium was 10 cm over an alfisol clay soil. Experimental plots were constructed using factorial completely randomized design with two native zoysiagrasses and 5 types of growing media. Two ecotypes of native zoysiagrasses showed different in growth habits combined with mixtures of growth media. Zim showed higher growing speed including more vigor with uniformity and texture than Zis. There were not significanthly differences on leaf color and root length between two ecotypes of native zoysiagrasses collected in Indonesia.

• Asian Journal of Turfgrass Science
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• v.16 no.2
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• pp.75-83
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• 2002

The back-filled soil of the New Incheon International Airport construction site was reclaimed with sea sand in near the Young-Jong island. The primary study was carried out from August 1993 to June 1997 to study soil amendment and to select salt resistance turfgrass species. This study dealt with low maintenance area that included most part of open space of airport site. The second experiment, from October 1996 to August 1998, focused on soil amendment and selection of turfgrass species for alongside runway where turf area was maintained. Through two previous studies, propagation methods with zoysiagrass were tested for alongside runway and surrounding areas at 1998. The study of construction methods with zoysiagrass, vegetative propagation showed better results on visual quality and cover rating compare with seeding propagation. However, significant different between vegetative and seeding propagation was not showed on visual quality and drought tolerance after one year of plot establishment. The cover rating by seeding construction methods reached in excess of 70% of limitation suggested by the Incheon International Airport Cooperation. Zoysia net and sprigging net methods were the most suitable where there requires rapid and high rate of ground cover. Seeding propagation should be acceptable to obtain a resonable cover rating where there allows relatively longer period of completion. Therefore, it should be possible to attain a proper rating of ground cover on the site of open space, alongside runway or areas similar to the New Incheon International Airport which is being built on dredged seashore sand. However, the methods of soil amendment, selecting salt tolerance species, and proper construction procedure should be considered at the a time.

• Weed & Turfgrass Science
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• v.3 no.2
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• pp.137-142
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• 2014

This study was conducted to investigate the effect of silicate mixed with nitrogen fertilizer on improving the growth and reducing nitrogen input of zoysiagrass. Plant height, fresh and dry weight of shoots, roots, and stolons, the number of shoots and total of stolons length were increased with highest in silicate mixed with nitrogen 24 kg/10a than nitrogen 24 kg/10a, and it showed no significance in silicate mixed with nitrogen 18 kg/10a. Nitrogen use efficiency in mixed silicate fertilizer was increased by 25-30% than single nitrogen fertilization. Moreover, the contents of available $SiO_2$, and organic matters of silicate fertilization on soil was higher than not silicate fertilization on soil. The silicate enhanced the growth and density of zoysiagrass, while it was a crucial factor to affect the chemical property of the soil.

• Weed & Turfgrass Science
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• v.2 no.4
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• pp.381-386
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• 2013

Recent climatic changes by global warming include increased amount and intensity of rainfall. This study was conducted to find out possible roles of turfgrasses to reduce the impact of climatic changes, especially surface soil erosion. Soil erosions by intensive rain were measured after each significant precipitation from the artificially sloped plots of zoysiagrass, cool-season grass mixture of Kentucky bluegrass and perennial ryegrass and other typical korean summer crops. Sodded zoysiagrass resulted in minimal annual soil erosion followed by strip-sodded zoysiagrass and cool-season turfgrass mixture while dry-field rice and bean cultivations eroded the surface soils of 5 to 10 MT $ha^{-1}yr^{-1}$ and pepper cultivation resulted in 7 to 14 MT $ha^{-1}yr^{-1}$ annual loss of surface soil. Annual loss of surface soil from bare land with hand weeding was up to 18 MT $ha^{-1}yr^{-1}$ while greatly reduced soil erosion was observed from weed grown treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.1
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• pp.20-24
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• 2003

Proline is known as an osmotrotectant to enhance tolerance against both salt and dehydration stresses. A P5CS ($\Delta^1$-pyrroline-5-carboxylate synthetase) plays a major role in regulation of synthesis of proline. An overexpression of the mothbean P5CS gene in transgenic tobacco plant increased the levels of proline and osmotolerance. In an attempt to look for the possibility to use content of proline as well as a level of P5CS gene expression as molecular markers for salt tolerance, the amounts of proline and transcript levels of P5CS were measured as functions of either concentration of NaCl or length of treatment period among different species of zoysiagrass. Hybridzoysia showed the highest level of proline ($329\mu\textrm{g}$/g.f.w.) among five different species of zoysiagrass at 250 mM NaCl in 24 hours. The level of P5CS transcript was also the highest in the hybridzoysia at 250 mM NaCl in 24 hours. The transcriptions of P5CS gene were induced at the rates of 1.2, 1.2, 1.8, and 1.8, upon treatment of 250 mM NaCl in Z. japonica, Z. matrella, Z. sinica and hybridzoysia respectively. Based on a correlation between the level of P5CS transcript and the proline content among different species of zoysiagrass, a comparative structural analysis of the gene for P5CS from either Z. sinica or hybridzoysia may lead to an understanding of mechanism for salt tolerance shown differently among zoysiagrasses.