• Journal of Ginseng Research
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• v.40 no.4
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• pp.453-461
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• 2016

Background: This study aims to describe the characterization of Paenibacillus polymyxa GBR-1 (GBR-1) with respect to its positive and negative effects on plants. Methods: The morphological characteristics of GBR-1 were identified with microscopy, and subjected to Biolog analysis for identification. Bacterial population and media optimization were determined by a growth curve. The potential for GBR-1 as a growth promoting agent, to have antagonistic activity, and to have hydrolytic activity at different temperatures was assessed. The coinoculation of GBR-1 with other microorganisms and its pathogenicity on various stored plants, including ginseng, were assessed. Results: Colony morphology, endospore-bearing cells, and cell division of GBR-1 were identified by microscopy; identification was performed by utilizing the Biolog system, gas chromatography of fatty acid methyl esters (GC-FAME). GBR-1 showed the strongest antagonistic activity against fungal and bacterial pathogens. GBR-1 cell numbers were relatively higher when the cells were cultured in brain heart infusion (BHI) medium when compared with other media. Furthermore, the starch-hydrolytic activity was influenced by GBR-1 at higher temperature compared to low temperatures. GBR-1 was pathogenic to some of the storage plants. Coinoculation of GBR-1 with other pathogens causes differences in rotting on ginseng roots. A significant growth promotion was observed in tobacco seedlings treated with GBR-1 suspensions under in vitro conditions, suggesting that its volatile organic compounds (VOCs) might play a role in growth promotion. Conclusion: The results of this study indicate that GBR-1 has both positive and negative effects on ginseng root and other stored plants as a potential biocontrol agent and eliciting in vitro growth promotion.

• Mycobiology
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• v.41 no.4
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• pp.225-233
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• 2013

Gibberella fujikuroi species complex (GFSC) was isolated from rice (Oryza sativa L.) seed samples from ten Asian countries and investigated for incidence of GFSC, molecular characteristics, and pathogenicity. Regardless of geographic origin, GFSC was detected with incidences ranging from 3% to 80%. Four species, Fusarium fujikuroi, F. concentricum, F. proliferatum, and F. verticillioides, were found to show an association with rice seeds, with F. fujikuroi being the predominant species. In phylogenetic analyses of DNA sequences, no relationship was found between species, isolates, and geographic sources of samples. Unidentified fragments of the ${\beta}$-tubulin gene were observed in ten isolates of F. fujikuroi and F. verticillioides. With the exception of three isolates of F. fujikuroi, F. fujikuroi, F. proliferatum, and F. verticillioides were found to have FUM1 (the fumonisin biosynthetic gene); however, FUM1 was not found in isolates of F. concentricum. Results of pathogenicity testing showed that all isolates caused reduced germination of rice seed. In addition, F. fujikuroi and F. concentricum caused typical symptoms of bakanae, leaf elongation and chlorosis, whereas F. proliferatum and F. verticillioides only caused stunting of seedlings. These findings provide insight into the characteristics of GFSC associated with rice seeds and might be helpful in development of strategies for management of bakanae.

• Journal of Bio-Environment Control
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• v.18 no.2
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• pp.171-176
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• 2009

Hydroponic culture has been developed to control soilborn diseases, to increase yield and to enhance the quality of vegetable, The pathogen could be detected from infected plant materials, hydroponic tanks, culture solution and solid media of the severely infected greenhouse, The density of pathogen population was coincided with the severity of disease incidence, For example, 1,900cfu m$L^{-1}$ pf pathogens were counted from tomato plants sampled in a 20% diseased greenhouse. The pathogens may be introduced in the greenhouse through the contaminated soil surrounding the house and/or through the infected young seedlings grown on the nursery soil. Also, not detected to Ralstonia solanacearum from tomato seeds (House Momotaro, Bbaebbae, Ggoggo, and Minicarol cultivar) selling at a market.

• Journal of Ecology and Environment
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• v.41 no.10
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• pp.281-289
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• 2017

Background: In this study, the growth and reproductive response of seedlings, grown in plastic pots with sand, to moisture and nutrients were analyzed in order to study the environmental conditions required to create an alternative habitat for Epilobium hirsutum L., an endangered plant. Results: Vegetative and reproductive growths of Epilobium hirsutum L. are accelerated with increase in moisture and organic matter content in the soil. Among vegetative organs, the number of runners related to asexual reproduction was the highest when the moisture content was over 25% and nutrient content between 7 and 14% in the soil. But the number of flowers related to flowering responses, among reproductive organs, was the highest when the moisture content was maintained at 75% and when nutrient content was 21% in the soil. The number of seeds, related to sexual reproduction, was the highest when the moisture content was over 25% and nutrient content between 14 and 21%. Conclusions: The study results show that a place with high moisture and nutrient content in the soil is advantageous to asexual and sexual reproduction of Epilobium hirsutum L. Therefore, we must serve periodically nutrient and seeds to sustain population in in situ conservation. Furthermore, it is advisable to create in riverside where abundant nutrient content have, making alternative habitat of Epilobium hirsutum L. Also, we must find species that have high contribution degree index through vegetation survey.

• Molecules and Cells
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• v.27 no.4
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• pp.409-416
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• 2009

ADAP is an AP2-domain protein that interacts with ARIA, which, in turn, interacts with ABF2, a bZIP class transcription factor. ABF2 regulates various aspects of the abscisic acid (ABA) response by controlling the expression of a subset of ABA-responsive genes. Our expression analyses indicate that ADAP is expressed in roots, emerging young leaves, and flowers. We found that adap knockout mutant lines germinate more efficiently than wild-type plants and that the mutant seedlings grow faster. This suggests that ADAP is involved in the regulation of germination and seedling growth. Both germination and post-germination growth of the knockout mutants were partially insensitive to ABA, which indicates that ADAP is required for a full ABA response. The survival rates for mutants from which water was withheld were low compared with those for wild-type plants. The result shows that ADAP is necessary for the response to stress induced by water deprivation. Together, our data indicate that ADAP is a positive regulator of the ABA response and is also involved in regulating seedling growth. The role of ADAP is similar to that of ARIA, which is also a positive regulator of the ABA response. It appears that ADAP acts through the same ABA response pathway as ARIA.

• Journal of Radiation Industry
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• v.6 no.2
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• pp.129-138
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• 2012

Proteomic analysis was performed in order to identify proteomic changes by salt stress between the Japonica cv. Donganbyeo (WT) and two salt-tolerant (ST) mutant lines by using the SDS-PAGE and 2-DE. Two salt tolerant rice mutant lines, ST-87 and ST-301, were selected by in vitro mutagenesis with gamma-ray. Three-week-old seedlings were treated with 171 mM NaCl for 7 days. In the SDS-PAGE, three proteins with molecular weights of 27, 46 and 58 kDa were highly increased under salt treatment. Total proteins from shoots of both WT and ST-lines were separated by two-dimensional gel electrophoresis. In 2-DE, 201, 226, 217 and 213 protein spots were detected in the untreated-or treated-WT and untreated- or treated-ST-87, respectively. Of theses, 17 and 10 protein spots were up- and down-regulated under salt stress in the WT, respectively. While, 16 and 8 protein spots were up- and down-regulated under salt stress in the ST-87, respectively, compared with the untreated plants. High intensity or de novo synthesized proteins were analyzed by MALDI-TOF/MS analysis.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.560-563
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• 2009

A new spray chrysanthemum(Dendranthema grandiflorum) cultivar, "Golden Eye" was developed from a cross between "Rosa" and "Angaesoguk" by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) from 2004 to 2008. Its characteristics were investigated three times from 2006 to 2008 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of "Golden Eye" was October 23th, and year-round production was possible by day length treatment. This cultivar was yellow in color, single in flower type for the spray type cut flower. Its capitulum was 2.4cm in diameter, and had 23.2 head per stem in autumn. Its ray floret was green central zone. The days to flowering under the short day treatment is about 49 days in spring season, and "Golden Eye" showed the vase life of 25.7 days in autumn. This cultivar was registered for a commercialization in 2008.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.295-298
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• 2010

A new spray chrysanthemum(Dendranthema grandiflorum) cultivar, 'Biz' was developed from a cross between 'S03-117' and 'Angaesoguk' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) from 2003 to 2008. Its characteristics were investigated three times from 2006 to 2008 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Biz' was October 26th, and year-round production was possible by day length treatment. This cultivar was white in color, single in flower type for the spray type cut flower. Its capitulum was 3.0 cm in diameter, and had 15.6 head per stem in autumn. Its ray floret was green central zone. The days to flowering under the short day treatment is about 42 days in spring season, and 'Biz' showed the vase life of 24.1 days in autumn. This cultivar was registered for a commercialization in 2008.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.229-233
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• 2008

A new spary chrysanthemum(Dendranthema grandiflorum) cultivar 'Moulin Rouge' was developed from a cross between 'Delmont' and 'Anjela' by selections of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Moulin Rouge' was October 24th, and year-round production was possible by day length treatment. Its capitulum was 5.3 cm in diameter, and had 22.9 ray florets and 12.2 head per stem in autumn. Its ray floret was bi-color(yellow/orange) with green central zone. 'Moulin Rouge' was about 55 days to flower in spring, and showed the vase life of 21.7 days in autumn. This cultivar was registered for a commercialization in 2007.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.200-204
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• 2008

A new spary chrysanthemum(Dendranthema grandiflorum) cultivar 'Serapina' was developed from a cross between 'Relanse' and 'Yen' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extention Services(ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Serapina' was October 23th, and year-round production was possible by day length treatment. Its capitulum was 2.8cm in diameter, and had 43.4 ray florets and 28.9 head per stem in autumn. Its ray floret was yellow with green central zone. To flower in the short day condition, for 'Serapina' was about 46 days in spring, and 'Serapina' showed the vase life of 21.7 days in autumn. This cultivar was registered for a commercialization in 2007.