• Title/Summary/Keyword: young seedlings

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Investigation of Ectomycorrhizal Fungal Colonization in Pinus thunbergii Seedlings at a Plantation Area in Gangneung, using Morphotyping and Sequencing the rDNA Internal Transcribed Spacer Region

  • Obase, Keisuke;Cha, Joo-Young;Lee, Jong-Kyu;Lee, Sang-Yong;Lee, Jin-Ho;Chun, Kun-Woo
    • Journal of Korean Society of Forest Science
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    • v.99 no.2
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    • pp.172-178
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    • 2010
  • The status of ectomycorrhizal (ECM) fungal colonization in Pinus thunbergii seedlings was investigated 2 years after planting in an eastern coastal area of Korea. We established three $10{\times}10$ m plots at a P. thunbergii plantation in Gangneung and sampled lateral roots from 10 seedlings in each plot. ECMs were classified into morphological groups and the number of root tips of each morphotype was counted. In total, 8 ECM morphotypes were observed and fungal species that form each morphotype were identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear rDNA. Suillus granulatus was the most abundant species (44.1-65.7% of relative abundance) in all plots, followed by Tomentella ellisii (14.0-37.8%) and unidentified fungus belonged to Atheliaceae (10.6-20.1%). These 3 fungal species accounted for almost all of the ECM abundance in each plot (94.9-99.8%). The remaining 5 fungal species were uncommon and rare. There was no clear difference in ECM fungal communities among plots. Community structure of ECM fungi in the young P. thunbergii plantation was simple and composed of fungal species that were also observed in mature coastal pine forests.

Growth, Protein and Pigment Content of Rice Seedlings under Phosphorus Deprivation Condition

  • Yun, Song-Joong;Park, Myoung-Ryul;Kim, Young-Doo;Kim, Key-Young;Baek, So-Hyeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.2
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    • pp.103-107
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    • 2003
  • Phosphorus (P) is a macronutrient playing important roles in many plant processes. Significant interest has been devoted to search and utilize genotypic variations in P use efficiency in rice but with little effort to understand its physiological and biochemical bases. In this study, we examined responses to P deprivation of some primary and secondary traits in 3-week-old seedlings of the three genotypes, Sobi-byeo (japonica), Dasan-byeo (japonica $\times$ indica) and Palawan (indica). In general, percent weight due to root was increased up to 26%, but amounts of root protein and proteins secreted from roots were decreased by 11 to 19% and 31 to 51 %, respectively, by 3 to 21 days of P deprivation in the three genotypes. Interestingly, however, responses of Palawan to short-term P deprivation were contrasting to those of Dasan-byeo and Sobi-byeo in seedling weight and contents of shoot protein, chlorophyll and anthocyanin. Seedling weight was not decreased, but shoot protein content was decreased in P-deprived seedlings of Palawan. Contents of chlorophyll in leaves and anthocynin in roots were increased in Dasan-byeo and Sobi-byeo, but decreased in Palawan. The results suggest that responses of protein and pigment synthesis to P deficiency are different in modem and traditional varieties and the difference may at least in part be due to the selection for high yield under highly fertilized conditions.

Vegetation Structure at the Slope Direction and Characteristic of Seedlings of Abies koreana in Hallasan Mountain (한라산 구상나무림의 사면별 식생구조와 치수발생 특성)

  • Song, Kuk-Man;Kang, Young-Je;Hyeon, Hwa-Ja
    • Journal of Environmental Science International
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    • v.23 no.1
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    • pp.39-46
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    • 2014
  • This study surveyed Abies koreana to identify the correlation between its vegetation structure according to its slope direction and seedling establishment in a bid to build basic research data on the changes and conservation of the A. koreana in Hallasan Mountain. The findings of its vegetation structure revealed that in both areas, Importance value was given to the A. koreana for its tree layer, the Taxus cuspidata for its shrub layer, and the Sasa quelpaertensis for its herb layer. However, in the Youngsil area with the tree layer, high importance was given to deciduous broad-leaved trees such as Prunus maximowiczii, Quercus mongolica, and the young species of the A. koreana in the shrub layer that can maintain the A. koreana forest's greater importance in the Jindallebat than in the Youngsil. Thus, the A. koreana forest in the Jindallebat is believed to lastlonger. The findings of correlation between the quantity of seedlings and their location by area revealed that in each tiny quadrat, the A. koreana seedling averaged 5.3 in the Youngsil and 2.9 in the Jindallebat. Both areas were all found to have a positive correlation in terms of rock exposure ratio and dead tree ratio as well as a negative correlation with regard to the cover degree of S. quelpaertensis, the canopy gap, the total vegetation, and the herb layer. It was found that the cover degree of the herb layer in the Youngsil and the S. quelpaertensis in the Jindallebat had the largest impact on the A. koreana seedlings.

Distribution of Fatty Acids in Newly Developed Tissues of Soybean Seedlings

  • Dhakal, Krishna Hari;Jeong, Yeon-Shin;Ha, Tae-Joung;Baek, In-Youl;Yeo, Young-Keun;Hwang, Young-Hyun
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.32-41
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    • 2011
  • The objective of this study was to determine the fatty acid composition of newly developed tissues of germinated soybean seeds. Five soybean accessions with varied fatty acid composition were allowed to germinate in sand under greenhouse conditions. Seedlings were picked up after 4, 6, 8, 10 and 12 days of germination and freeze dried. The fatty acid composition of the newly developed tissues was analyzed by gas chromatography. Significant variation in fatty acid composition was observed between accessions, days of germination, and variety ${\times}$ day of germination in whole and the cotyledons. In the case of newly developed five tissues, significant variation in fatty acid composition were observed between days of germination except oleic acid for root, hypocotyl and epicotyl stem and except stearic acid for hypocotyl and unifoliate leaves while all the parameters were significantly different for accession. Significant interactions of accession and days of germination were observed for palmitic, linoleic and linolenic acid in all tissues; only for oleic acid in hypocotyl, epicotyl and unifoliate leaves; and only for stearic acid in root, hypocotyl, epicotyl and unifoliate leaves. During germination, the fatty acid composition of newly developed tissues changed dramatically but whole seedlings and cotyledons changed slightly. These tissues contained five major fatty acids as found in original seeds, but compositions were totally different from that of the seed: higher in palmitic, stearic and linolenic acid and lower in oleic and linoleic acid. New tissues conserved their fatty acid compositions regardless of genotypic variation in the original seeds.

A comparative analysis of characteristics and antioxidant capacity of Korean mulberries for efficient seedling cultivation

  • Chan Young Jeong;Heon Woong Kim;Seong Ryul Kim;Hyun-Bok Kim;Kee-Young Kim;Seong-Wan Kim;Jong Woo Park;Ik Seob Cha;Sang Kug Kang;Ji Hae Lee
    • International Journal of Industrial Entomology and Biomaterials
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    • v.47 no.2
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    • pp.147-154
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    • 2023
  • Mulberry exhibits unique characteristics and functionalities across various components, including the roots, branches, leaves, and fruits. However, despite numerous studies on mulberry, research on this plant at the seedling stage is insufficient. Therefore, this study aimed to compare the suitability for seedling cultivation and antioxidant effects of four Korean mulberry cultivars, namely, Daesim, Suhong, Simgang, and Cheongsu. In terms of seed weight, germination rate, and growth rate, Daesim was the most suitable cultivar for seedling production. Polyphenol and flavonoid content analysis showed that all cultivars, except for Cheongsu, showed the highest phenolic content at the 2-week seedling stage. Similarly, antioxidant assays using 2,2'-azino-di-3-ethylbenzthiazoline sulfonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals revealed that the antioxidant effect of all cultivars, except for Cheongsu, increased with cultivation at 2- 4- and 6-week. However, mulberry seedlings had a slower reaction rate against DPPH radical removal than mulberry leaves. In addition, ABTS radical scavenging activity showed a different correlation with polyphenol content. This phenomenon may be due to the different polyphenol compositions between mulberry leaves and seedlings. The results of this study suggest that mulberry seedlings exhibit different bioactivities from mulberry leaves, and component analysis is required in further research.

Regeneration Process of the Korean Beech (Fagus Multinervis Nakai) Forest

  • Kim, Seong-Deog
    • 한국생태학회:학술대회논문집
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    • 2002.08a
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    • pp.133-139
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    • 2002
  • Regeneration process of the Korean beech (Fagus multinervis Nakai) forest was studied in relation to the distributional pattern and age structure. In this study, it was clarified that the Korean beech forest is regenerated in both way: seedlings(sexual reproduction) and sprouts(vegetative reproduction) formations. In the large gaps, seedling may be established, resulting in clusters of even-aged individuals. But in the small-sized gaps, there may filed by the extention of surrounding trees and/or by the young trunks, which have sprouted from the base of stumps. As most gaps in the F. multinervis forest were derived from falling of a single stem, regeneration by sprouts seems to be more common than that by the seedlings.

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