• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.665-673
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• 2012

A balance trial experiment was carried out to evaluate the potential relationship between an enzymatically hydrolyzed yeast (EHY) and yeast culture combined with a live Bacillus subtilis (Bs) on the productive parameters, ileal digestibility, retention of nutrient and energy and villus morphology in broilers. Seventy two 28 d old, Ross B308 male broilers were assigned to a factorial combination of 2 levels of EHY (0 and 1 kg/ton of feed) and 2 levels of Bs (0 and 125 g/ton of feed). The experiment lasted 2 weeks. Several treatment interactions were observed. EHY-fed broilers showed the lowest feed intake and feed conversion ratio whereas Bs-fed broilers showed the highest feed intake and intermediate feed conversion ratio (EHY and BS interaction, p<0.05). Also, EHY-fed broilers had greater ileal digestibility of dry matter (EHY and BS interaction, p<0.01) and energy (EHY and BS interaction, p<0.05) but these responses were counterbalanced by the combination of EHY and Bs. The thickness of the mucosa was similar between the control and EHY-fed broilers, but was lowest when Bs was added alone (EHY and BS interaction, p<0.01). The thickness of the villus was greater in EHY plus Bs-fed broilers, intermediate for the control and lower for Bs or EHY-fed broilers (EHY and BS interaction, p<0.05). The area of the villus was greater in the control and EHY plus Bs-fed broilers (EHY and BS interaction, p<0.05). In addition, EHY-fed broilers showed greater breast yield and nitrogen retention (p<0.01) and ashes digestibility (p<0.05). On the other hand, Bs-fed broilers had greater carcass and breast weight, nitrogen retention, energy excretion and villus height (p<0.05). In summary, EHY and Bs enhanced some growth, carcass and nutrient retention responses, but did not show any synergic relationship in these responses. Opposite to this, the results suggest that the positive effect of EHY on the feed conversion and digestibility of nutrients were counterbalanced by the addition of Bs.

• 한국유기농업학회지
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• 제23권3호
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• pp.523-534
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• 2015

본 연구는 미생물제제의 일종인 yeast culture 및 코팅 처리된 vitamin-C의 첨가 급여를 통해 비육 말기 Holstein 거세우의 증체 및 도체형질에 미치는 영향을 조사하기 위한 목적으로 실시하였다. 평균체중이 $714{\pm}13.60kg$인 18개월령 Holstein 거세우 24두를 공시동물로 이용하였으며 각각 8두씩 3개 시험구로(대조구, MC급여구, CVC급여구) 완전임의 배치하여 출하 전 3개월간 사양시험을 실시하였다. MC급여구는 yeast culture를 일일 30 g, 코팅 처리된 vitamin-C는 일일 10 g씩 매일 아침 사료급여 시 top dressing형태로 급여하였다. 일당증체량은 MC급여구가 가장 높았으며 사료요구율은 MC급여구와 CVC급여구에서 가장 낮았다. 등지방두께, 배체장근 및 육량은 시험구간 유의차가 없었다. 지육중량과 성숙도는 MC급여구에서 가장 높았으며, 지방색은 CVC급여구에서 가장 높게 나타났다. 이상의 결과를 종합해 보면 출하 3개월 전 Holstein 거세우에게 항생제 대체제로 미생물제제인 yeast culture 또는 코팅 처리된 vitamin-C의 급여는 육질에는 큰 효과가 없으나 육량, 지육중량, 사료요구율 등에 긍정적인 영향을 줄 수 있을 것으로 판단된다. 아울러 미생물제제와 vitamin-C를 함께 급여하면 더욱 좋은 효과가 있을 것으로 예상된다.

• 생명과학회지
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• 제6권2호
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• pp.135-141
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• 1996

양식을 위한 먹이사료로서 가능성 있는 효모균주를 연구하였다. Kluyveromyces fragilis효모와 Candida utilis 효모의 최대 비증식 속도와 바이오매스 효율은 Saccharomyces cervisiae 효모에 비하여 굉장히 높은 값을 나타내었다. 따라서, 이 연구는 대량생산을 위하여, 플라스크 배양을 통한 이들 두 효모균들의 성장특성에 그 초점을 맞추었다. 5% 접종량을 가지고 실험한 결과, 가장 좋은 $\mu$$_{max}$ 와 OD$_{max}$ 값들은 K, fragilis는 2.5% fructose 배지에서, C. utilis는 2% YE배지에서 각각 얻었으며, 이 때의 이 값들은 K. fragilis는 0.73 hr$^{-1}$1 와 3.00을 C. uitilis는 0.59 hr$^{-1}$ 와 2.80으로 밝혀졌다. 또한, 아연과 염의 초기종도가 증가할수록 균성장에 있어서의 우도기는 증가하였으며, 접종량이 증가할수록 유도가는 감소하였다. 두 효모균은 3.5% 염농도에서 비교적 잘 자랄 수 있었으며, C. utilis 효모균만이 아연을 이용할 수 있었다.

• 미생물학회지
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• 제19권2호
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• pp.63-77
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• 1981

The growth rate of yeast population (Saccharomyces uvarum) cultivated in the Knopp's modified medium (plus various carbon sources) appeared the highest value when the Knopp's minimal medium was treated to 1.5% with disaccharide such as maltose and sucrose. Also the treatment of lactose and raffinose resulted in polulation growth as to the population size in case of maltose and sucrose. However, the gorwth of yeast was not occurred at all when a polysaccharide, such as inulin, was added as carbon source. The growth from of yeast population in Knopp's modified medium are characterized by the fact that log phase continued 100hrs after inoculation and that stationary state phase appeared in general 250hrs after inoculation. Applying the various carbon sources to respiration substrate for yeast cell, the respiration rate of yeast showed the highest value in treatment of maltose and followed in order of raffinose, lactose, glucose, and sucrose. Determined the amount of poly-phosphate and turn over pathway of poly-phosphate according to culture phase of yeast, it is revealed that the yeast synthesized 3 types of poly phosphate (poly-P A,B, and C) and postulated that turn over pathway of poly-phosphate as follows ; Inorganic phosphate is converted into each kind of polyphosphates, and then one part of poly-P-C is converted into poly-P-B, the rest poly-p-C and poly-P-B are converted into poly-P-A. The synthesized poly-phosphate is considered to have a role as energy pool utilizing to synthesis of cellular organic materials. Of the 13 carbon sources used in this experiment, the useful carbon sources for biosynthesis of poly-phosphate and cellular organic materials are confirmed as disaccharide (maltose and sucrose) as well as glucose. Protein synthesis in yeast cell showed the two peaks on 6th and 8th day after inoculation ; nucleic acid on 2nd day (48hrs), carbohydrates on 2nd day (48hrs), and phospholipid on 2nd and 8th day after inoculation, respectively.

• 대한예방한의학회지
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• 제13권2호
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• pp.89-102
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• 2009

Objectives : The purpose of this study was evaluated hypoglycemic effect of culture broth of germanium-fortified Tricholoma matsutake mycelium and yeast. Methods : We examined $\alpha$-glucosidase inhibitory activity, blood glucose level, concentration of serum lipid, and serum metabolic variables of Tricholoma matsutake mycelium and yeast fortified Germanium. in streptozotocin induced diabetic rat. Results : In the $\alpha$-glucosidase inhibitory activity, germanium-fortified yeast was significantly higher than germanium-fortified Tricholoma matsutake mycelium. The hypoglycemic effects of germaniumfortified Tricholoma matsutake mycelium was higher than germanium-fortified yeast. The activity of alkaline phosphatase(ALP), aspartate aminotransferase(AST) and alanine aminotransferase(ALT) was significantly lower in the germanium-fortified Tricholoma matsutake mycelium and yeast than in diabetic control(DC) group and diabetic positive control(PC) group. The concentration of total cholesterol and triglyceride of germanium-fortified yeast was significantly lower than germanium-fortified Tricholoma matsutake mycelium, DC group and PC group. Conclusions : The results suggest that germanium-fortified Tricholoma matsutake mycelium and yeast have improvement effects in blood glucose, serum lipid and liver function.

• 산업식품공학
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• 제15권4호
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• pp.413-419
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• 2011

쌀단백질을 이용한 고부가가치 천연 조미소재를 개발하기 위하여 쌀단백질을 프로테아제로 효소분해한 배지에서 Saccharomyces cerevisiae를 배양하여 제조한 효모 추출물(Yx)에, 효소분해 후 남은 쌀단백질 잔사를 Bacillus licheniformis 혹은 Bacillus subtilis로 발효하여 얻은 발효물(Rfl, Rfs)을 각각 첨가하였다. 쌀단백질 잔사의 발효물이 첨가된 효모추출물(YxRfl, YxRfs)의 전체적 선호도는 첨가전의 효모추출물(Yx)에 비하여 높았으며, 특히 쌀단백질 발효물의 보충에 의해서 감칠맛과 같은 풍미가 증가함을 미각센서 분석 및 관능검사에 의해서 확인할 수 있었다. 쌀단백질 잔사발효물에 의한 감칠맛의 상승은 감칠맛을 내는 아미노산 이 외에도 쌀단백질의 발효에 따라 유리된 다양한 펩타이드 분획의 영향이 있었을 것으로 예상된다. 이와 같이 감칠맛 아미노산 및 펩타이드가 함유된 쌀단백질 발효물이 보충된 효모추출물은 감칠맛과 풍미의 상승작용으로 전체적인 기호도가 높아짐에 따라 고부가가치 천연조미소재의 제조에 응용될 수 있을 것으로 기대되었다.

• Applied Biological Chemistry
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• 제19권4호
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• pp.219-226
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• 1976

옥수수 전분박을 이용하여 식사료 효모를 생산하기 위하여 전분박의 산당화법을 검토하고 이 산당화액을 이용하여 liquid culture에서 식용효모, semisolid culture에서 사료효모를 생산하기 위한 배양조건을 검토한 바 다음과 같은 결과를 얻었다. 1) 옥수수 전분박의 신당화시 가수분해제로는 염산이 황산보다 효과적이었으며, 염산 농도 1%, 압력 $2.0kg/cm^2$에서 30분간 가수분해 시켰을 때 당화율이 57.2%로서 가장 좋았다. 2) 산분해시 원료와 전분박의 비가 1:10 이상에서는 분해가 증가되지 않았으며 semisolid substrate를 만들기 위해서는 1:3의 비율이 적당하였다. 3) 6종류의 효모를 전분박 당화액에 배양시켰을 때 Candida tropicalis가 가장 좋았으며, 최적 초기 pH는 6이었으며, 최적온도는 $30^{\circ}C$였다. 4) Liquid culture에서 Candida tropicalis의 무기 영양 요구도를 조사한 결과 $(NH_2)_2CO$ 0.3%, $KH_2PO_4$, 0.15%, $MgSO_4{\cdot}7H_2O$ 0.04%였으며, semisolid culture시에는 $NH_4CI$ 0.4%, $KH_2PO_4\;0.1%\;MgSO_4{\cdot}7H_2O\;0.04%$를 가했을 때가 좋았다. 5) 식용 효모생산을 위한 액체배양에서는 초기당 4%에서 88.75%를 소비하였으며, 원료에 대한 건조효모로써 19.13%의 수율을 보였다. 6) 사료효모를 생산하기 위해 semisolid한 상태에서 배양하였을 때 처리하지 않은 전분박에 비해cellulose는 14.7%에서 3.76%로 감소하였으며, 건조효모는 13.89%가 생성되었다.

• 생약학회지
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• 제27권4호
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• pp.289-294
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• 1996

In previous paper, we described the induced callus of Corydalis remota contains a significant amount of alkaloids. This study describes an optimal condition to maximize alkaloid production. The suspension cultures maintained alkaloid production ability after fifth subculture and a small amount of alkaloid seemed to be released out of cells. The yields of alkaloid by cultured cells was varied depending on the concentrations of NAA, carbon sources and phosphate ion and depending on the vitamin combinations and concentrations. Biosynthetic precursor and an elicitor treatment also affected the total alkaloid yield of the cultures. The optimal conditions for alkaloid production were as follows: 1) MS basal salt containing 30 g/l of glucose, 1.0 mg/l of NAA, and vitamins of LS medium should be used. 2) The culture should be treated with tyrosine 20 mg/l, and yeast extract 1.5 ml/l after the culture reached a stationary phase of growth. Five alkaloids were isolated from the cultures and they were characterized. The spectral data unambiguously revealed that the isolated compounds were dihydrosanguinarine, protopine. tetrahydropalmatine, allocyptopine and ambinine, respectively.

• 한국식생활문화학회지
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• 제30권4호
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• pp.475-480
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• 2015

Jeju citrus, which contains an abundance of calcium and vitamin, was used to develop fermented citrus peel extract. A total of seven probiotic strains were applied to tangerine dermis to select the best growing bacteria in citrus peel extracts. B. longum, B. bifidum, and L. mesenteroides were found to grow best in citrus peel extract culture containing glucose, yeast extracts, peptone, and potassium phosphate. Citrus peel extract culture consisting of 1% yeast extract, 5% peptone, and 0.1% phosphate was the best environment for growth of probiotics. The pH, acidity, and viable cell numbers of these fermented extracts were measured. The initial pH level of fermented extracts with nutrients was 5.25 and dropped rapidly to 3.39 after 72 hours of fermentation. The acidity of fermented extracts increased to 4.08 % after 72 hours of fermentation, and the viable cell number in fermented extracts after refrigeration for 2 weeks was $1.3{\times}10^{10}CFU/mL$. The antimicrobial activity of citrus peel fermented extracts against Campylobacter jejuni was determined, and concentrations more than 25,000 ppm showed antimicrobial activity.

• 한국식품과학회지
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• 제22권6호
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• pp.696-699
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• 1990

쌀을 기질로 하여 고오지 곰팡이와 Saccharomyces cerevisiae를 함께 동시 접종하여 혼합 배양에 의한 동시 당화발효를 시도하였다. Aspergillus awamori, A. kawachii, A. niger, A. oryzae 및 A. shirousamii를 각각 S. cerevisiae와 혼합배양하였을 때 A. shirousamii와의 혼합배양에서 가장 높은 주정생산량을 보였다. 이 때의 가수량은 쌀 50g에 대하여 150ml 이었다. 곰팡이와 효모는 각각 $5{\times}10^2\;conidia/ml$ 및 $5{\times}10^6\;cells/ml$로 접종한 경우에, 용적에 대한 표면적의 비는 0.1에서 그리고 초기 pH6.5 및 $30^{\circ}C$의 배양조건에서 10일 동안 발효시 최고 12.9%의 주정이 생산되었다.