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High-density genetic mapping using GBS in Chrysanthemum

  • Chung, Yong Suk;Cho, Jin Woong;Kim, Changsoo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.57-57
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    • 2017
  • Chrysanthemum is one of the most important floral crop in Korea produced about 7 billion dollars (1 billion for pot and 6 billion for cutting) in 2013. However, it is difficult to breed and to do genetic study because 1) it is highly self-incompatible, 2) it is outcrossing crop having heterozygotes, and 3) commercial cultvars are hexaploid (2n = 6x = 54). Although low-density genetic map and QTL study were reported, it is not enough to apply for the marker assisted selection and other genetic studies. Therefore, we are trying to make high-density genetic mapping using GBS with about 100 $F_1s$ of C. boreale that is oHohhfd diploid (2n = 2x = 18, about 2.8Gb) instead of commercial culitvars. Since Chrysanthemum is outcrossing, two-way pseudo-testcross model would be used to construct genetic map. Also, genotype-by-sequencing (GBS) would be utilized to generate sufficient number of markers and to maximize genomic representation in a cost effective manner. Those completed sequences would be analyzed with TASSEL-GBS pipeline. In order to reduce sequence error, only first 64 sequences, which have almost zero percent error, would be incorporated in the pipeline for the analysis. In addition, to reduce errors that is common in heterozygotes crops caused by low coverage, two rare cutters (NsiI and MseI) were used to increase sequence depth. Maskov algorithm would also used to deal with missing data. Further, sparsely placed markers on the physical map would be used as anchors to overcome problems caused by low coverage. For this purpose, were generated from transcriptome of Chrysanthemum using MISA program. Among those, 10 simple sequence repeat (SSR) markers, which are evenly distributed along each chromosome and polymorphic between two parents, would be selected.

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Pre-service Elementary Teacher' Knowledge understanding and Teaching-learning type about 'stratum and rock' ('지층과 암석'에 대한 초등 예비 교사의 지식 이해와 교수유형)

  • Lee, Yong-Seob;Kim, Soon-Shik;Lee, Ha-Lyong
    • Journal of the Korean Society of Earth Science Education
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    • v.6 no.1
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    • pp.69-77
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    • 2013
  • The study aims to figure out pre-service elementary teachers' knowledge understanding on 'stratum and rock' as well as teaching-learning types on the same topic. A total of 65 seniors in an advanced science education course at B University of Education joined the research to fulfill the purpose above. With PCK classification framework, the study examined pre-service teachers' knowledge understanding on 'stratum and rock' while it analyzed how the teachers would teach the given topic to students. The results of the study are presented as follows. First, it was observed that the pre-service elementary teachers have a great understanding on 'stratum and rock' that would be taught via a science textbook for elementary fourth graders. However, regarding terms in 'shale and limestone', they appeared to have a relatively short understanding. Second, PCK elements of the pre-service teachers related to 'stratum and rock' were analyzed and according to the results, the teachers would be interested in teaching model selecting in the teaching-learning strategy field while they would be well aware of how important it is for them to perform an experiment in a teaching process. The teachers also appeared to understand that the teacher question can be mutual complementary during class. However, it turned out that the teachers would have a very much low understanding on learners' prior knowledge as they particularly believe that learning could be significantly affected by the learners' perception level as well as their learning interest and motive. Third, the pre-service elementary teachers were told to design teaching plans on 'stratum and rock' so that the study could find out what learning-teaching methods the teachers would adopt to teach the topic. It was learned that the teachers would proceed with the class basically by giving the learners a descriptive explanation on the topic and also by using pictures and drawings to enhance the learners' understanding during the class.

The Analysis of the Characteristic Types of Fashion Brand Application - Concentrating on Korean Application cases - (패션 브랜드 어플리케이션의 특징적 유형 분석 - 한국 계정 어플리케이션 사례를 중심으로 -)

  • Park, Min-A;Ko, Hyun-Zin
    • Journal of the Korean Society of Costume
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    • v.64 no.1
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    • pp.136-151
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    • 2014
  • This study systematically analyzed types of fashion brand application focusing on accounts created in Korea. While referring to 'Chanel' which has developed a fashion brand app for the first time in August of 2008, not only for App store by Apple Inc. of the greatest market share but also for Android market, the one and only competitor of App store, the study examined cases of fashion brand app in Korea and foreign countries which have been in service till August of 2013 since the year of 2008. To achieve the research goal, the study conducted a literature research and a case review, categorizing the app by their distinctive functions which were Basic Information, SNS, AR, LBS, Entertainment, Mobile Shopping and Live Streaming. As for the first function, Basic Information, it was considered to provide information on a brand such as prices, sizes and colors of products which should be the most fundamental function of a fashion brand. The function would include look book, catalogues, photographs and others of products, helping users of the app with their understanding on images and concepts of the brand. Second, SNS function was considered useful for its mobility and communication and with the help of theirs, the users share fashion information with each other. Third, AR function as in a filed of virtual reality would edit virtual objects to look real in an actual environment. This would eventually offer the users a chance to try for clothes virtually. The fourth function, LBS, would work with GPS to find a store closest from a present location. This would be a help when the users try to find stores holding promotion events or trails while hiking in mountains. The fifth Entertainment function would include all sorts of games and chances for the users to listen to music and keep fashion diaries. The sixth function, Mobile Shopping, would help the users purchase items online via the app as they would not visit a store in person. The seventh function, Live Streaming, would give the users chances to actually see fashion collections in real time, held all over the world in every season. Because of this function, not only fashion experts but also regular people have become able to enjoy the fashion shows. The distinctive characteristics of the fashion brand application discussed in the study will be a useful reference when any relevant fields try to design other new fashion brand application.

Dynamic Analysis on the Construction of the Innovative City (혁신도시 건설에 관한 동태적 분석)

  • Lee, Man-Hyung;Kim, Yeon-Sik;Kim, Mi-Sung;Hong, Sung-Ho
    • Korean System Dynamics Review
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    • v.8 no.2
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    • pp.141-173
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    • 2007
  • In Korean context, the innovative cities imply new towns or urban clusters in the Non Capital Regions, all of which would accommodate the relocated Capital based public organizations. The central government-initiated innovative cities have provoked pros and cons towards their effectiveness and efficacy for the balanced territorial development. From a broader prospective, this paper firstly examines the current status quo of the innovative cities. Based on their master plans, it analyzes physical and non physical factors which would exert significant impact on the innovative cities. Secondly, it pays attention to how key factors strengthen or weaken their behavioral patterns in terms of dynamic location and implementation policies of the innovative cities. Using System dynamics approaches, it sets up couples of scenarios, categorizing between supporting and opposing arguments towards the innovative cities. Lastly, after divulging systematic structure of the innovative cities, it proposes a series of practical alternatives which would contribute to minimizing unexpected side effects or unwanted social cost in the long run. In order to guarantee reinforcing structure of the innovative cities, the paper suggests that QOL (quality of life) variables, which would require continuous investment in the social infrastructure, are pivotal in achieving original goals of the innovative cities. Otherwise, the innovative cities would not be innovative per se. In the worst case, they might be degraded into the unpopular ghost towns.

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Effects of Age, Sex, and Friendship Type on Child's Shared Knowledge of Friend (연령·성·친구관계 유형이 친구에 대한 아동의 공유지식에 미치는 영향)

  • Kim, Young Hee
    • Korean Journal of Child Studies
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    • v.9 no.1
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    • pp.33-46
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    • 1988
  • The purpose of this study was to investigate the effects of age, sex, and friendship type on child's shared knowledge of a friend. The following hypotheses were raised; Child's shared knowledge of friend would increase with age, shared knowledge of girls would be higher than that of boys shared knowledge of girls would be higher than that of boys shared knowledge in a mutual friendship would be higher than in a unilateral friendship, there would be an interaction effect between age and friendship type in child's shared knowledge of friend. The subjects of this study consisted of 80 boys and girls, 8- and 11-year-olds. Shared knowledge of a friend was measured with a modified version of the 70 picture items developed by Ladd and Emerson (1984). The obtained data were analyzed by three-way ANOVA. Child's shared knowledge of a friend increased with age. Girls exhibited greater ORSK (other-referenced shared knowledge) than did boys, but there was no significant difference between girls and boys in SRSK (the selfreferenced shared knowledge). Mutual friendships exhibited greater SRSK than did unilateral friendships, but there was no significant difference between mutual friendship and unilateral friendship in ORSK. There was a significant interaction effect between age and friendship type in child's shared knowledge of friend.

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An Empirical Study on Public Child Care Center for the Necessity of the Eco-Environmental Space (친환경공간의 필요성에 따른 구립 어린이집의 사례 연구)

  • Han, Hae-Ryon;Park, Young-Ki
    • Proceedings of the Korean Institute of Interior Design Conference
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    • 2007.05a
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    • pp.91-94
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    • 2007
  • The change of modern society arouse the issue of the family structure and the demand of upbringing. According to the issue, the need of the upbringing is the big interest in society. This study is on the necessity of the eco-environmental space in public child care center. The study progressed with the result of the prior study, the demand of the parents of students in eco-environmental space in child care center. The results of the study are first, the natural light source can import in sky light, side light and atrium in child care center. Second, the indoor garden and interior plants would give the natural feelings. Third, the ponds and waterways in indoor and outdoor would give the empirical experiences. And the last, the stone and clay would give the tender feelings in interior and the sand play in outdoor would give the activities. This study would be the foundation on the upcoming study of the needs of the eco-environmental spaces in child facilities.

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The Study on the Experience of Clinical Nursing Practice among Nursing Students using Grounded Theory Approach. (근거이론적 접근방법을 적용한 간호학생들의 임상실습 경험)

  • Suh Moon-Sook;Kim Myoung-Hee;Chon Mi-Young
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.3 no.1
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    • pp.128-140
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    • 1996
  • This study is to understand and to explain how nursing students experience in the clinical nursing practice using grounded theory approach. Ten nursing students were participated in the study. Seven of them were junior students at the junior college of nursing, and the rest were the senior students at the university. The data were collected by in-depth individual interviews by investigators during May and June in 1995. The results of the study were as follows ; Twenty-eight concepts and 9 categories were emerged by the constant comparative analysis. The 9 categories include 'the need of role model', 'non-educational practical setting', 'knowledge deficit', 'emotional changes', 'the attitudes of clinical practice', 'fatigue', 'skepticism', 'pride', and 'the product of clinical practice'. The core category which encompasses all 9 categories was emerged as 'the process of formulating the nursing view'. Five hypotheses were derived from the analysis. 1) The desirable role model would enhance to pride of nursing in students. 2) Non-educational practical setting would increase skepticism of nursing in students. 3) Knowledge deficit would negatively affect on experience of clinical practice. 4) The pride of clinical practice would formulate a positive nursing view. 5) The skepticism of clinical practice would formulate a negative nursing view. The results of this study are to use as basic data for students attending clinical experience.

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The Research on the Disposal of Unused Cloths -Focusing on the Environmental Protection- (착용하지 않는 의복의 처리실태에 관한 조사 연구 -환경보전을중심으로-)

  • 김병미
    • Journal of the Korean Home Economics Association
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    • v.35 no.2
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    • pp.19-32
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    • 1997
  • The purpose of this research is to find out ways of disposing unused cloths focusing on the environmental protection. To achieve its aim, previous researches are studied and simultaneously a survey has been done for housewives in Taejeon city. Collected data have been analyzed by the SPSS program. The result of this research is as follows. On the questions concerning planned clothes buying, meaningful differences are obtained according to their ages, degrees of education, occupations, patterns of housing. On the period wearing cloths, meaningful difference is shown only by the age. the first reason for disposing of unused cloths is that the cloths become unfit for the body, second is that the designs or colors become so old that the consumer does not like it any more. On the ways of currently disposing of unused cloths are very much different from on the ways of desirable disposal of unused cloths. On the questions of recycling, the experience of wearing used cloths given by others is high. On the question of 'what would you do if you were given used cloths?', the commonest answer is 'I would wear them because it would be beneficial economically.', 'I wouldn't wear them.', 'I would wear them because it would be any help for resource frugality or pollution prevention'. On the question of the experience of getting used cloths voluntarily, 38.1 percentage of pollees has answered yes, 62 percentage no.

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Analysis of R&D Investment for Hydrogen and Fuel Cell (수소.연료전지 연구개발 투자현황 분석)

  • Park, No-Eon;Kim, Hyung-Wook
    • Transactions of the Korean hydrogen and new energy society
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    • v.21 no.2
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    • pp.143-148
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    • 2010
  • Research and Development (R&D) investment of hydrogen and fuel cell, funded by government from 2007 to 2008 in Korea, has been analyzed. R&D investment of hydrogen and fuel cell in 2008 would see 9% and 29% of total budget in the field of renewable energy, respectively. It was found that R&D investment is mainly dependent on mission of Ministry in Korea. Basic and apply research would be mainly invested by Ministry of Education, Science and Technology (MEST), while development research would be conducted by Ministry of Knowledge Economy (MKE). In R&D investment by performer, hydrogen technology would be conducted by government-funded institute and university. It was also shown that funds for hydrogen production have been much supported than hydrogen storage. Meanwhile, fuel cell would be mainly conducted by major companies. It was also shown that funds for proton exchange membrane fuel cell (PEMFC) have been much invested than other technology in fuel cell.

A Study on the Future Direction of Fashion Design Major in College (전문대학 패션디자인전공 발전 방향에 관한 연구)

  • Han, Gyung-Hee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.16 no.2
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    • pp.211-225
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    • 2014
  • Globalization in the 21st century and development in the field of IT have caused rapid change in the practical site of fashion industry. Therefore, as colleges aim to produce professional technicians and experts in the applicable fields, they are strengthening professionalism and practicality more than ever before that can respond to the practical situation. In addition, owing to the reduction of population of academic students, colleges would search for specialty and structural innovation with the serious desire to establish new structural system to produce talented persons. Purpose of this study is to strengthen competition of colleges voluntarily, specialize themselves and maximize mutual benefit through cooperative relation with industrial businesses. Thus, this study would compare, analyze and investigate the curriculums of fashion designs in colleges for the plan of curriculum applicable for the industrial changes and corresponding business demand. Considering the result of questionnaires of industrial businesses, most of them would prefer the curriculum for 3 years to that for 2 years. Nowadays industrial businesses will require computerization ability much more than ever before besides the professional knowledge and technology in the field of fashion and it is also showed that education of personality in colleges would be considered to be important for the performance of the vocational duty. Especially, specialty and progressive participation in the curriculum would be strengthened during the education process of colleges. This study will be used for the establishment of specialty of colleges and development of curriculum of them.

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