• Korean Journal of Veterinary Research
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• v.36 no.2
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• pp.483-494
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• 1996

The present study was undertaken to identify the species of nematodes and determine infection state of small intestinal nematodes from dogs in Taegu area. One hundred and eleven healthy dogs which were 8 months old or more were examined by means of autopsy from September to November, 1993. All of the worms in small intestine were collected and preserved in 10% formalin solution. The worms were cleared in the lactophenol solution and examined by stereomicroscope and lightmicroscope. The result obtained were summarized as follows : Fifty three dogs(47.7%) out of the 111 experimental dogs were infected with small intestinal nematodes. Hookworm infection was recognized in 30(27.7%) dogs. All of the hookworms were identified morphologically as Ancylostoma caninum which had 3 pairs of ventral teeth, a pair of triangular dorsal teeth and a pair of centrolateral teeth. No Uncinaria stenocephala infection was detectable. Roundworm infection was recognized in 37(33.3%) dogs. Twenty one(18.9%) dogs were infected with Toxascaris leonina which had characteristic dorsal lips, paired spicules and a simple tail without process. The individual worm burden of the infected dogs ranged from one to 361 with mean of 25.5%. Among them 0~358(mean $17.8{\pm}30.66$) were Ancylostoma caninum, 0~18(mean $1.6{\pm}3.44$) were Toxocara canis and 0~60(mean $6.0{\pm}12.05$) were Toxascaris leonina. Single infection with Ancylostoma caninum, Toxocara canis and Toxascaris leonina were observed in 16(14.4%) dogs, in 9(8.1%) dogs, and in 11(9.9%) dogs respectively. Double infection with Ancylostoma caninum plus Toxascaris leonina and Toxocara canis plus Toxascaris leonina occurred in 5(0.5%) and in 3(2.7%) dogs respectively. Triple infection with Ancylostoma caninum plus Toxocara canis plus Toxascaris leonina occurred in 7(6.3%) dogs.

• Parasites, Hosts and Diseases
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• v.26 no.1
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• pp.9-14
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• 1988

In an attempt to determine the susceptibility of inbred golden hamsters(Mesocricetus auratus) to the experimental infection with Clonorchis sinensis, twenty-five hamsters were divided into 5 groups and administered orally 5, 10, 20, 30 or 50 metacercariae each. The hamsters were killed on the 45th day after infection. The adult flukes were recovered from all hamsters. The overall recovery rate for the fluke was 57.9% with the range of 48.4% to 92.0%. As the number of the metacercariae given increased, a porportionate decrease in the recovery rate was found, the prepatent period of the fluke in the hamster varied from 15 to 17 days, with the average of 16 days. No significant differences in the development of the flukes were observed. Egg production expressed by EPG, increased step by step as the time elapsed. However, a tendency of decreasing egg laying capacity was observed with the increase of worm burden. It is suggested that the hamster is a suitable final host of C. sinensis.

• Parasites, Hosts and Diseases
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• v.51 no.2
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• pp.155-163
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• 2013

This study aimed to evaluate the efficacy of fructose-1,6-bis phosphate aldolase (SMALDO) DNA vaccination against Schistosoma mansoni infection using different routes of injection. The SMALDO has been cloned into the eukaryotic expression vector pcDNA3.1/V5-His TOPO-TA and was used in injecting Swiss albino mice intramuscularly (IM), subcutaneously (SC), or intraperitoneally (IP) ($50{\mu}g/mouse$). Mice vaccinated with non-recombinant pcDNA3.1 served as controls. Each group was immunized 4 times at weeks 0, 2, 4, and 6. Two weeks after the last booster dose, all mice groups were infected with 80 S. mansoni cercariae via tail immersion. At week 8 post-infection, animals were sacrificed for assessment of parasitological and histopathological parameters. High anti-SMALDO IgG antibody titers were detected in sera of all vaccinated groups (P<0.01) compared to the control group. Both the IP and SC vaccination routes resulted in a significant reduction in worm burden (46.2% and 28.9%, respectively, P<0.01). This was accompanied by a significant reduction in hepatic and intestinal egg counts (41.7% and 40.2%, respectively, P<0.01) in the IP group only. The number of dead eggs was significantly increased in both IP and IM groups (P<0.01). IP vaccination recorded the highest significant reduction in granuloma number and diameter (54.7% and 29.2%, respectively, P<0.01) and significant increase in dead miracidia (P<0.01). In conclusion, changing the injection route of SMALDO DNA vaccination significantly influenced the efficacy of vaccination. SMALDO DNA vaccination via IP route could be a promising protective and antipathology vaccine candidate against S. mansoni infection.

• Parasites, Hosts and Diseases
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• v.26 no.3
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• pp.199-206
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• 1988

An epidemiological study was performed to know the infection status of 5. hortense among the human residents and fish intermediate hosts in Chongsong·gun, Kyongsangbuk·do. Out of 263 people examined, 59(22.4%) were positive for the eggs of E. hortense. Total 152 fresh water fishes (9 species) were ellamined, and Odentobutis obscura interrupts, Misgurnus an- guiIZicaudatus and Squalidus coreanus were found to harbour the metacercariae of E. hortense. Juvenile or adult E. hertense were expelled from 35 out of 54 cases treated with pragiquantel. The number of worms Per individual ranged from 1 to 649, with the average burden of 51. The eosinophil count of infected persons ranged 1∼24% (6% in average). The number of eggs per day Per worm (EPDPW) in human host was estimated about 1,478. The results showed that Chongsong-gun is an endemic area of E. hortense.

• Parasites, Hosts and Diseases
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• v.44 no.1 s.137
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• pp.1-5
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• 2006

One coastal village in Haenam-gun and two in Yeongam-gun, Jeollanam-do were surveyed for intestinal parasite infections by fecal examination. The egg positive rates of Gymnophalloides seoi were high, $24.1\%$ (14/58) in Haenam-gun and $9.3\%$ (11/118) in Yeongam-gun. The egg positive rates of heterophyids, including Heterophyes nocens, and of Clonorchis sinensis were $10.3\%\;and\;6.9\%$ in Haenam-gun, and $14.4\%\;and\;8.5\%$ in Yeongam-gun, respectively. After praziquantel treatment and purgation, a total of 37,761 fluke specimens were recovered from 17 patients; 11 in Haenam-gun and 6 in Yeongam-gun. Gymnophalloides seoi was the most commonly recovered species, with 37,489 specimens in total (2,205 per person). Other recovered flukes included Heterophyes nocens, Stictodora fuscata, Heterophyopsis continua, Pygidiopsis sUmma, and undetermined species. These results indicate that the areas surveyed are new endemic foci of G. seoi.

• The KIPS Transactions:PartC
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• v.16C no.1
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• pp.13-20
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• 2009

Recently, as traffic flooding attacks such as DoS/DDoS and Internet Worm have posed devastating threats to network services, rapid detection and proper response mechanisms are the major concern for secure and reliable network services. However, most of the current Intrusion Detection Systems (IDSs) focus on detail analysis of packet data, which results in late detection and a high system burden to cope with high-speed network traffic. In this paper we propose an SNMP-based lightweight and fast detection algorithm for traffic flooding attacks, which minimizes the processing and network overhead of the detection system, minimizes the detection time, and provides high detection rate. The attack detection algorithm consists of three consecutive stages. The first stage determines the detection timing using the update interval of SNMP MIB. The second stage analyzes attack symptoms based on correlations of MIB data. The third stage determines whether an attack occurs or not and figure out the attack type in case of attack.

• Parasites, Hosts and Diseases
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• v.32 no.3
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• pp.157-162
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• 1994

A highly endemic focus of human infection with Heterophves nocens (Heterophyidae) was discovered from a small coastal village of Shinan-gun, Chollanam-do, for the first time in Korea. Fecal examinations by cellophane thick smear and formalin- ether sedimentation techniques revealed 42.9% heterophyid egg Positive rate out of 98 inhabitants examined. It was difficult to confirm the species of heterophylds only by eggs. In order to collect the adult flukes, the egg Positive cases were treated with 10 mg/kg single dose of praziquantel and purged with magnesium sulfate, and the adult worms were collected from the diarrheic stools. From each of 18 cooperative patients 1 to 1,124 (total 4,730) H. nocens worms were recovered, together with a few to large numbers of heterophyids (Pvginiopsis summa, Stictonora juscatunl and for gymnophallids (Gvmopholloides stool) . It is speculated that H. nocen might be widely distributed along the southwestern coastal areas where the brackish water fish such as the mullets or gobies are popularly eaten raw.

• Parasites, Hosts and Diseases
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• v.61 no.3
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• pp.251-262
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• 2023

Schistosomiasis causes significant morbidity and mortality worldwide. This study aimed to assess the effect of schistosomula lung antigen preparation (SLAP) and soluble egg antigen (SEA) on a murine schistosomiasis mansoni model. Ninety laboratory-bred male Swiss albino mice were divided into 6 groups. Two doses of the vaccine were given at 2-week intervals. All mice were subcutaneously infected with 80±10 Schistosoma mansoni cercariae 2 weeks after the last vaccination dose. They were sacrificed 7 weeks post-infection. Parasitological and histopathological studies were conducted to assess the effect of inoculated antigens (single or combined). The results showed that the combination of SLAP and SEA (combination group) led to a significant reduction in worm burden (65.56%), and liver and intestine egg count (59% and 60.59%, respectively). The oogram pattern revealed a reduction in immature and mature eggs (15±0.4 and 10±0.8, respectively) and an increased number of dead eggs in the combination group (P<0.001). In terms of histopathological changes, the combination group showed notably small compact fibrocellular egg granuloma and moderate fibrosis in the liver. A high percentage of destroyed ova was observed in the intestine of the combination group. This study demonstrates for the first time the prophylactic effect of combined SLAP and SEA vaccine. The vaccine induced a significant reduction in the parasitological and pathological impacts of schistosomiasis mansoni in hepatic and intestinal tissues, making it a promising vaccine candidate for controlling schistosomiasis.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.142-149
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• 1983

Epidemiological studies on the reinfetion pattern of Ascaris lumbricoides were undertaken by means of blanket mass chemotherapy and worm collection in a rural village in Korea, during 1977~1980. The study objectives were to determine the age (sex)-specific reinfection rate during 2, 4, 6 and 12 months through repeated mass chemotherapy with pyrantel pamoate, and to observe the familial aggregation tendency of the reinfected cases. The results obtained are as follows: 1. The age (sex)-reinfection curve revealed that the reinfection rate is much higher in younger individuals than in olders in all of 4 kinds of interval chemotherapy groups. The highest reinfection rate and the highest burden of reinfected worms were observed in preschool children, followed by primary school students. Such fluctuation in the age-specific reinfection rates was more pronounced in males than in females. 2. There was noted a significant tendency of familial aggregation among the reinfected cases. It is suggested that reinfection occurs never randomly but preferably to the members of certain household families. From these reinfection analyses, it is inferred that the principal mode of A. lumbricoides transmiSSIOn in the surveyed rural area is likely to be of 'dooryard type', in which case children and certain family members are more preferably reinfected. It is also suggested that the preschool children should be included in the primary targets of mass control programme.

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.101-112
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• 1991

While various examples of rationale or benefits of ascariasis control could justify a necessity of control activity in a community, few practical index other than the e99 Prevalence or intensity of infection has been used to evaluate the efficacy obtained by the control activity. A literature-based retrospective study was undertaken to provide some numerical data on the decreasing morbidity of surgical ascariasis as a consequence of ascariasis control in Korea. A thorough review of literatures (1959∼1990) on biliary and other surgical complications of ascariasis reported from general hospitals revealed that their incidence has been decreasing very proportionally to the national figures of its e99 Prevalence and worm burden. For example, according to the remarkable decreases of the prevalence of ascariasis, there is an evident decreasing tendency of the proportion(%) of ciliary ascariasis cases among all biliary surgical patients and the proportion of biliary ascariasis cases among all biliary stone patients in general hospitals. It is highly suggested that the Korean people have been much benefited by the control activity of ascariasis executed at a national scale since 1960s, especially in terms of incidence of biliary and other surgical complications of ascariasis.