• Korean Journal of Microbiology
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• v.54 no.1
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• pp.60-68
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• 2018

A cold-active and alkaline serine protease (Pro21717) was partially purified from the Antarctic marine bacterium Pseudoalteromonas arctica PAMC 21717. On a zymogram gel containing skim milk, Pro21717 produced two distinct clear-zones of approximately 37 kDa (low intensity) and 74 kDa (high intensity). These were found to have identical N-terminal sequences, suggesting they arose from an identical precursor and that the 37 kDa protease might homodimerize to the more active 74 kDa form of the protein. Pro21717 displayed proteolytic activity at $0-40^{\circ}C$ (optimal temperature of $40^{\circ}C$) and maintained this activity at pH 5.0-10.0 (optimal pH of 9.0). Notably, relative activities of 30% and 45% were observed at $0^{\circ}C$ and $10^{\circ}C$, respectively, in comparison to the 100% activity observed at $40^{\circ}C$, and this enzyme showed a broad substrate range against synthetic peptides with a preference for proline in the cleavage reaction. Pro21717 activity was enhanced by $Cu^{2+}$ and remained stable in the presence of detergent surfactants (linear alkylbenzene sulfonate and sodium dodecyl sulfate) and other chemical components ($Na_2SO_4$ and metal ions, such as $Ba^{2+}$, $Mg^{2+}$, $Ca^{2+}$, $Zn^{2+}$, $Fe^{2+}$, $K^+$, and $Na^{2+}$), which are often included in commercial detergent formulations. These data indicate that the psychrophilic Pro21717 has properties comparable to the well-characterized mesophilic subtilisin Carlsberg, which is commercially produced by Novozymes as the trademark Alcalase. Thus it has the potential to be used as a new additive enzyme in laundry detergents that must work well in cold tap water below $15^{\circ}C$.

• Science of Emotion and Sensibility
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• v.12 no.3
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• pp.317-324
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• 2009

The aim of this study was to determine whether or not cardiovascular reactivity parameters serve as good indicators in identifying differential emotion in children. The study particularly focused on five emotions(i.e., happiness, sadness, anger, stress, and boredom), thus, study participants were introduced to a combination of music, color, stories, and dolls to induce complex emotions. During the experiment, corresponding cardiovascular reactivity in response to the conditioned stimuli were recorded on physiological parameters including HR, RSA, HRV, HF HRV, LF HRV, and FPV. After the cardiovascular reactivity responses were measured, participants rated on the types and intensity of emotions they had experienced during the emotional stimuli exposure. Results on psychological response show that four emotions except for stress were appropriately and effectively induced participants by emotional stimuli. Findings of physiological responses suggest that, except for RSA, all of the physiological indicators show significant differences among five emotions. This indicates that children' emotions can be measured and differentiated by cardiovascular reactivity, or in other words, emotion specific responses have the ability to distinguish different emotions in children.

• Journal of the Korea Computer Graphics Society
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• v.22 no.3
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• pp.21-29
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• 2016

Cell segmentation is an important but time-consuming and laborious task in biological image analysis. An automated, robust, and fast method is required to overcome such burdensome processes. These needs are, however, challenging due to various cell shapes, intensity, and incomplete boundaries. A precise cell segmentation will allow to making a pathological diagnosis of tissue samples. A vast body of literature exists on cell segmentation in microscopy images [1]. The majority of existing work is based on input images and predefined feature models only - for example, using a deformable model to extract edge boundaries in the image. Only a handful of recent methods employ data-driven approaches, such as supervised learning. In this paper, we propose a novel data-driven cell segmentation algorithm for bright-field microscopy images. The proposed method minimizes an energy formula defined by two dictionaries - one is for input images and the other is for their manual segmentation results - and a common sparse code, which aims to find the pixel-level classification by deploying the learned dictionaries on new images. In contrast to deformable models, we do not need to know a prior knowledge of objects. We also employed convolutional sparse coding and Alternating Direction of Multiplier Method (ADMM) for fast dictionary learning and energy minimization. Unlike an existing method [1], our method trains both dictionaries concurrently, and is implemented using the GPU device for faster performance.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.08a
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• pp.189-189
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• 2012

Lead sulfide (PbS) nanocrystal quantum dots (NQDs) are promising materials for various optoelectronic devices, especially solar cells, because of their tunability of the optical band-gap controlled by adjusting the diameter of NQDs. PbS is a IV-VI semiconductor enabling infrared-absorption and it can be synthesized using solution process methods. A wide choice of the diameter of PbS NQDs is also a benefit to achieve the quantum confinement regime due to its large Bohr exciton radius (20 nm). To exploit these desirable properties, many research groups have intensively studied to apply for the photovoltaic devices. There are several essential requirements to fabricate the efficient NQDs-based solar cell. First of all, highly confined PbS QDs should be synthesized resulting in a narrow peak with a small full width-half maximum value at the first exciton transition observed in UV-Vis absorbance and photoluminescence spectra. In other words, the size-uniformity of NQDs ought to secure under 5%. Second, PbS NQDs should be assembled carefully in order to enhance the electronic coupling between adjacent NQDs by controlling the inter-QDs distance. Finally, appropriate structure for the photovoltaic device is the key issue to extract the photo-generated carriers from light-absorbing layer in solar cell. In this step, workfunction and Fermi energy difference could be precisely considered for Schottky and hetero junction device, respectively. In this presentation, we introduce the strategy to obtain high performance solar cell fabricated using PbS NQDs below the size of the Bohr radius. The PbS NQDs with various diameters were synthesized using methods established by Hines with a few modifications. PbS NQDs solids were assembled using layer-by-layer spin-coating method. Subsequent ligand-exchange was carried out using 1,2-ethanedithiol (EDT) to reduce inter-NQDs distance. Finally, Schottky junction solar cells were fabricated on ITO-coated glass and 150 nm-thick Al was deposited on the top of PbS NQDs solids as a top electrode using thermal evaporation technique. To evaluate the solar cell performance, current-voltage (I-V) measurement were performed under AM 1.5G solar spectrum at 1 sun intensity. As a result, we could achieve the power conversion efficiency of 3.33% at Schottky junction solar cell. This result indicates that high performance solar cell is successfully fabricated by optimizing the all steps as mentioned above in this work.

• Journal of Adhesion and Interface
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• v.17 no.4
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• pp.141-148
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• 2016

In this work, mesoporous silica (SBA-15) was synthesized via self-assembly process using triblock copolymer ($PEO_{20}PPO_{70}PEO_{20}$, P123) as template and tetraethyl orthosilicate (TEOS) as silica source under acidic condition. SBA-15 have high surface area ($704m^2g^{-1}$) and uniform pore size (8.4 nm) with well-ordered hexagonal mesostructure. Spiropyran-functionalized SBA-15 (Spiropyran-SBA-15) was synthesized via post-synthesis process using 3-(triethoxysilyl)propyl isocyanate (TESPI) and 1-(2-Hydroxyethyl)-3,3-dimethy-lindolino-6'-nitrobenzopyrylo-spiran (HDINS). Spiropyran-SBA-15 was produced with hexagonal array of mesopores without damage of mesostructre. Surface area and pore size of Spiropyran-SBA-15 were $651m^2g^{-1}$ and 8.0 nm, respectively. Optochemical properties of Spiropyran-SBA-15 was studied with chemical vapors such as EtOH, THF, $CHCl_3$, Acetone and HCl. Main peaks of photofluorescence of Spiropyran-SBA-15 exhibited blue shift in the range of 603.4~592.1 nm after exposure under EtOH, THF, $CHCl_3$, and Acetone vapors. Normalized peak intensities decreased in the range of 0.8~0.3. The main peak of photofluorescence of Spiropyran-SBA-15 showed significant blue shift of 592.1 nm after exposure under HCl vapor, while normalized peak intensity decreased to 0.1.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.06a
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• pp.23-23
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• 2009

GaN-based nitride semiconductors have attracted considerable attention in high-brightness light-emitting-diodes (LEDs) and laser diodes (LDs) covering from green to ultraviolet spectral range. LED and LD heterostructures are usually grown on (0001)-$Al_2O_3$. The large lattice mismatch between $Al_2O_3$ substrates and the GaN layers leads to a high density of defects(dislocations and stacking faults). Moreover, Ga and N atoms are arranged along the polar [0001] crystallographic direction, which leads to spontaneous polarization. In addition, in the InGaN/GaN MQWs heterostructures, stress applied along the same axis can also give rise to piezoelectric polarization. The total polarization, which is the sum of spontaneous and piezoelectric polarizations, is aligned along the [0001] direction of the wurtzite heterostructures. The change in the total polarization across the heterolayers results in high interface charge densities and spatial separation of the electron and hole wave functions, redshifting the photoluminescence peak and decreasing the peak intensity. The effect of polarization charges in the GaN-based heterostructures can be eliminated by growing along the non-polar [$11\bar{2}0$] (a-axis) or [$1\bar{1}00$] (m-axis) orientation instead of thecommonly used polar [0001] (c-axis). For non-polar GaN growth on non-polar substrates, the GaN films have high density of planar defects (basal stacking fault BSFs, prismatic stacking fault PSFs), because the SFs are formed on the basal plane (c-plane) due to their low formation energy. A significant reduction in defect density was recently achieved by applying blocking layer such as SiN, AlN, and AlGaN in non-polar GaN. In this work, we were performed systematic studies of the defects in the nonpolar GaN by conventional and high-resolution transmission electron microscopy.

• The Korean Journal of Quaternary Research
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• v.18 no.2 s.23
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• pp.107-107
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• 2004

In this paper we have introduced some results of study on stand growth pattern and stand structure of larch forest which are located in selected forest sites of Khangai and Gobi-Altai mountain ranges of Mongolia. Our investigations showed that growth intensity and stand structure in western Mongolia are very specific from the other forest vegetation zones of Mongolia. Studies on the stand structure and growth trend indicate that tree types of stand structure and different types of growth of Larix sibrica are very common in Western Mongolia. These peculiarities of stand structure and growth of larch stands in Western Monolia could be used for inventory work and an improvement of the forest management in Western Mongolian region. The larch tree is the dominant tree species in Western Mogolia. Forest cover of the region is about 15%, which is two times higher than the country's average. In this region forest area is divided into 4 forest sub-regions: the Central Khangai, Western Khangai, North Eastern Khangai and South Easterun Khangai sub-regions including taiga, pseudo taiga, sub taiga, sub-alpine and forest steppe belts. Silviculture practices and forest research management request to study forest growth trends in local and general conditions, which means to indicate a change of taxonomic characteristics of stand from time to time including diameter, height, basal area, growth stock etc. The forest management practice mostly uses tables of forest growth and yield based on the results of long term research on forest growth. Forest yield tables and other relevant forest standards of Russia are used for the forest inventory and forest management. They are not able to determine forest structure and growth peculiatities of Mongolian forests. Studies on forest resource assessment in Mongolia indicate that after logging operations and forest fires the natural regeneration of desired species such as pine and larch often does not succeed. This situation forces to take a different approach of forest management and silviculture practice depending on the stand structure and growth rate of the forest stands. According to our investigation in last years, forest growth pattern of larch forest depends mostly on stand structure, stand age and growth condition including forest soil, climate and location in different slopes. Due to improve environmental function of forest ecosystem in the region, it is needed to conduct very comprehensive study of high mountain forest ecosystem in selected sub-regions.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.10
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• pp.3772-3778
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• 2010

The leaders such as The Robert Foundation of the U.S., Social Firms U.K., EMES European Research Network worldwide are groping for the survival strategies of social enterprises and of their developmental methods with the utilization of social capital. Along with the way the world economy goes on, this study is first of all to empirically analyze how the diversity and strength of network as independent variables work with the studies of the survival of enterprises of Granovetter Mark, Burt Ronald, Coleman James, Peter Witt, Andreas Schroeter, Christin Merz, Helen Haugh, mainly concerned with the increase in employment, the increment in sales, delegation of authorization as dependent variables and secondly it is to present a theoretical possibility of optimizing the development of social enterprises. The object of this study consists of 25 companies recommended by experts out of the current national 295 social enterprises in 2009 through the analysis of sources of SPSS 12.0, appropriateness, reliability, interrelation, etc; besides, hypotheses are proved by multiple regression analysis. A result of the investigation indicates that there is the necessity of network in all the processes of the survival of enterprises, the growth in employment, the increase in sales, delegation of authorization; especially, it suggests that it is necessary to manage, maintain and develop primary factors relating to a variety of networks to improve sales, and relating to the intensity of network for the survival of corporations. At last, I think that this study could be a help to the strategies of utilizing social capital in order for many companies or nonprofit social organizations in Korea to develop into constant enterprises.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.254-263
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• 2020

This study developed indexes of farmers' acceptance factors for new farming technologies and evaluated the importance of the developed. For this study, farming technologies were classified according to the criteria established by the Rural Development Administration, and the Delphi technique was applied to evaluate the importance of the indexes. The indexes were composed of two tiers and evaluated by the analytic hierarchy process (AHP). Results showed that the importance of the first tier was finance and profitability with 43.9%, technology affairs 23.6%, labor environments 22.4%, and related industries and service system 10.1%. The importance of the second tier was the ease of technology acceptance 64.9% and technology completion 35.1% for technology affairs in the first tier, labor intensity 53.7%, work hazard 26.7%, working hours 19.6% for labor environment, farm income increment 69.9% and new fund required 30.1% for finance and profitability, and training and service system 66.7% and activation of related industries 33.7% for related industry and service system. This study may contribute to farmers' acceptance of new farming technologies and increase the efficiency and effectiveness of agricultural research and extension activities by the Rural Development Administration.

• The Journal of the Korea Contents Association
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• v.9 no.10
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• pp.451-463
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• 2009

The purpose of this research is to develop thin film materials and fabrication process for efficient $TiO_2$/CdTe solar cells. In this work photocatalyst titanium dioxide was prepared by sol-gel procedure according to reaction condition, the mole ratio of $H_2O$/TTIP, pH of solution and aging condition of powder. The prepared titanium dioxide was thermally treated from 300 to $750^{\circ}C$. Maximum intensity of anatase phase of titanium dioxide was achieved by calcination at $600^{\circ}C$ for 2 hr. And it was mixture of anatase and rutile phase when temperature of calcination was $750^{\circ}C$. It has been known that the properties of synthesized titanium dioxide according to aging time and calcination temperature was converted to anatase phase crystal on increasing of aging time. Also the current density has been increased with aging time and temperature, the efficiency has been increased with because of reason on above results. The formation of chemical bonding on oxygen and cadmium telluride under oxygen circumstances had been observed, and oxygen of thin film surface on cadmium telluride had been decreased with the treatment of chromate and hydrazine. As results had been shown that the energy conversion efficiency of cadmium telluride use by rapidly treatmented heat at the condition of $550^{\circ}C$ under air circumstance got 12.0%, 6.0% values according to $0.07cm^2$, $1.0cm^2$ surface area, respectively.