• Journal of Plant Biotechnology
• /
• v.37 no.4
• /
• pp.343-356
• /
• 2010

Plant micropropagation techniques include bud cultures using apical or axillary buds, organogenesis through callus culture or adventitious bud induction, and somatic embryogenesis. In Korea Forest Research Institute (KFRI), the first tissue culture trial in woody plant was initiated from the bud culture of hybrid poplars (Populus alba x P. glandulosa) in 1978. Since then several mass propagation techniques have developed from conifer and hardwood species, resulting in allowing practical application to Poplars, Birches and some oak species. In addition, useful micropropagation and genetic resources conservation techniques were established in some rare and endangered tree species including Abeliophyllum distichum. Among various in vitro propagation techniques, somatic embryogenesis is known to be the most efficient plant regeneration system. Since the first somatic embryo induction was reported in Tilia amurensis by KFRI in 1986, various protocols for direct or indirect somatic embryogenesis systems have developed in conifer and hardwood species including Larix leptolepis, Pinus rigida x P. taeda F1, Kalopanax septemlobus and Liliodendron tulipifera, etc. However, most of these technologies have been developed using juvenile tissues, i.e. immature zygotic embryos or mature embryos. Therefore it has been difficult to directly application to tree breeding program due to their unproven genetic background. Recently remarkable progresses and new approaches have been achieved in mature tree somatic embryogenesis. In this article we reviewed several micropropagation techniques, which have been mainly developed by KFRI and recent international progresses.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.3
• /
• pp.99-107
• /
• 1965

Thirteen varieties of hemp, Cannabinus sativa L., consisting of four Italian, one Japanese and eight local varieties were used in the study on the fiber content and a satisfactory selection for the higher-fiber-yielding plants. In addition, the relationship among fiber contents at the ball setting stage, and at the maturity stage, primary fiber content, and total fiber content were determined. The results of the study are summarized as follow: 1. Fiber weight of the matured male plant expressed as percentage of the fiber weight to dried stems varied from 12.09 to 26.71 percent for the Italian varieties, 14.25% for the Japanese variety and ranged from 6.59 to 16.79 for the local varieties. It was shown that on the average, Italian varieties contained more fiber compared with the other varieties. 2. On the same basis, the average percent age of fiber for Italian male plants was 20.77 while it was 20.96 for female plants. For the Japanese variety, similar figures were 14.24 for male plants and 15.43 for female plants. By comparison, the average percent fiber for the male plants of Korean varieties was only 10.34 but averaged 16.79 for female plants. In this experiment, female plants contained a greater percentage of fiber than male plants. 3. Statistically significant correlations were obtained between fiber content at the ball setting stage and fiber content of the mature plant. The correlation coefficient was r=0.942＊＊, while the regression equation was Y=0.28+1.03X. These data indicate that plant. selection by early testing at the ball setting stage is an effective method for the improvement of fiber content. 4. The correlation between primary fiber content and total fiber content was r=0.913＊＊, with a regression equation of Y=12.01+1.20X. It should thus be possible to obtain plants of higher total fiber content at maturity by the selection of plants which have a higher content of primary fiber at the ball setting stage. 5. From a study of the transverse sections from the hemp plant it was shown that Italian varieties have wide bands of primary and secondary fibers with narrow areas of woody material, while both Korean local varieties and Japanese varieties have narrow bands of primary and secondary fiber and wide areas of woody material. For hemp improvement, it is certain that a variety which has a narrow area of woody substances and wide area of fiber in its stem should be selected.

• The Korean Journal of Ecology
• /
• v.16 no.4
• /
• pp.477-487
• /
• 1993

To investigate phenological differences among species, and relationship between phenology and air temperatures, we surveyed foliation and flowering times of several woody plants in two temperate forests, Namhansansung and Taegwallyong area, for three years, 1991, 1992 and 1993. In Namhansansung area, the leaves of Quercus mor~golica, Rhododendron mucronulatum, Prunus levezlleana and Symplocos chinensis for. pilosa expanded in the early season(about 10 April), and those of Quercus variabilis, Quercus dentata and Maackia amurensis in the late season(about 5 May). The foliation time of the earliest species(Rhododendron mucronulatum) was 27 days earlier than thzt of the latest(Maackia amurensis, Quercus variabilis and Quercus dentata). In Taegwallyong area, the leaves of Staphylea bumalda and Rhamnus yoshinoi foliated on 25 April and those of Rhus verniciflua and Fraxinus rhynchophylla on 25 May. The annual mean air temperature of Narnhansansung area was $5.5^{\circ}C$ higher than that of Taegwallyong area. Foliation times of the same species were earlier in the former: the differences between two areas were 8~24 day among species. In contrast, flowering times of the same species were 0~22 days earlier in the former. It is concluded that the budding time of leaves was related to year day index(YDI), and foliation time of leaves was related to Nuttonson's index(Tn).

• Journal of Korean Society of Forest Science
• /
• v.97 no.2
• /
• pp.156-164
• /
• 2008

Recognizing the importance of the multi-purpose management of natural deciduous forest, this study was carried out to implement the partial cutting for stand regulation to examine agroforestry practice as well as other concurrent forest resource production, and to investigate the changes in stand characteristics and understory vegetation in a silvopasture practiced natural deciduous stand in the Research Forest of Kangwon National University, Korea. Three different partial cutting intensities (68.1%, 48.6%, and control) were performed in the unmanaged natural deciduous stand in order to improve the growing condition, especially light condition, for introducing some commercial herbaceous plants on the forest floor to establish agroforestry and/or silvopastoral system. Dominated by Quercus varibilis Blume (50.5%) and Quercus dentata Thum. ex Murray (42.6%), eight tree species were composed of the study forest, including poles of Pinus desiflora Siebold & Zucc and sapling of Pinus Koraiensis Siebold & Zucc. The total of 87 (13 tree species, 12 shrub species, 58 herbaceous species, and 4 woody climbers) vascular plant species were observed in study site after partial cutting treatments, while that of before partial cutting was 53 species (14 tree species, 8 shrubs species, 30 herbaceous species, and 1 woody climbers). The proportion of life form spectra in plot B was Mi (28.4%)-Na (23.0%)-Ge (17.5%)-Ch (10.8%)-He (9.5%)-MM (6.7%)-Th (4.1%). No statistically significant differences were observed in changes of life form spectra from before to after partial cutting treatment and among partial cutting gradients in this study. Partial cutting and scratching for forage sowing made plants invade easily on the forest floor, and light partial cutting (LPC) plot (500 stems/ha) had much higher number of undersory species than those of heavy partial cutting (HPC) plot (310 stems/ha) and control plot (1,270 stems/ha).

• The Korean Journal of Mycology
• /
• v.45 no.4
• /
• pp.345-354
• /
• 2017

The leaves of two woody plant species, Pinus densiflora and Aronia melanocarpa, were collected in Korea, and endophytic fungi were isolated from these surface-sterilized leaves. The fungal isolates were identified based on their morphological characteristics and the results of the phylogenetic analysis involving nucleotide sequences of the internal transcribed spacer region (ITS), including 5.8S rDNA, D1/D2 regions of 28S rDNA, and ${\beta}-tubulin$ genes. Pestalotia lawsoniae and Zasmidium fructicola were isolated from Pinus densiflora, and three species, Pestalotiopsis chamaeropis, Pestalotiopsis jesteri, and Stagonosporopsis cucurbitacearum were isolated from Aronia melanocarpa. To the best of our knowledge, these species have not been previously reported in Korea.

• The Korean Journal of Ecology
• /
• v.17 no.1
• /
• pp.37-47
• /
• 1994

A study was conducted to examine the leaf expansion forms and to analyze the leaf growth in early growing season of 1992 in a temperate deciduous forest in central region of Korea. After the winter bud scale fell off, the expansion forms of 11 woody species were divided into 3 groups, spreading fan form, opening form from half folding, and unrolling form from main vein. The ratios of leaf area at the end of growing season to that of leaf expantion time varied among species, and were related closely to expansion forms. The leaves reached to full size between the third ten days of April and the middle ten days of May, except for a few species. Leaf weight, however, increased steadily during the growing season. Specific leaf area (SLA) increased rapidly for 10-20 days after leaf expansion and decreased rapidly for 10 days after reaching maximum values, and thereafter decreased slowly. The SLA values of trees were smaller than $200cm^2/g$, but those of subtree and shrub were larger than $200cm^2/g$.

• Korean Journal of Agricultural Science
• /
• v.23 no.2
• /
• pp.182-187
• /
• 1996

Herbal and woody plants were surveyed in native and artifitial stands of experimental forest and stand within the vicinity of belongings facilities in Chungju Experimental Forest, Chungnam National University. Appearance species were classified and taken a photograph of. There were a total of 135 kinds, 60 familia, 113 genus, 123 species, 12 variatas. 49 kinds of them were herbs, 86 kinds were woody plants, and 7 kinds were evergreen trees. We try to solve the problem that species of plant could be sustained going on and be diversified.

• Asian-Australasian Journal of Animal Sciences
• /
• v.32 no.9
• /
• pp.1439-1447
• /
• 2019

Objective: The aim of this study was to evaluate the effect of dietary incorporation of tanninrich woody species on meat oxidative stability, carcass traits and meat quality in goats. Methods: Two tannin-rich species were tested using a three-treatments feeding trial, where treatments consisted of: Larrea divaricata and Acacia aroma both at 12.5% in dry matter basis of the diet and a control diet (alfalfa hay). All feeding diets were iso-protein and iso-energy. Carcass conformation, carcass compactness, carcass fatness and subcutaneous fat deposition were evaluated. Intake, liveweigh, Longissimus thoracis et lumborum muscles of goats were analyzed in order to evaluate quality parameters such as pH value, instrumental color evaluation, water holding capacity, total phenolic content, antioxidant activity, meat oxidative stability and fatty acid profiles in meat. Results: Feed intake, liveweight gain, carcass, and meat traits did not differ among treatments. Changes in meat lipid profile among treatments were observed for oleic and elaidic acid contents. Meat total phenolic content and antioxidant activity did not differ among treatments; although, meat oxidative status after storage at room temperature, as well as under refrigerated and frozen conditions were different between control and both supplemented groups. Conclusion: The inclusion of Acacia aroma and Larrea divaricata leaves in goat diet enhanced meat oxidative stability. Modulation of the ruminal biohydrogenation of fatty acids produced by condensed tannins of these plant species need to be further investigated.

• Forest Science and Technology
• /
• v.14 no.4
• /
• pp.181-191
• /
• 2018

This study was carried out in the teaching and research forest of the University of Dschang in Belabo, with the aim of analysing land-cover and land-use changes as well as carbon stocks dynamic. The databases used are composed of three Landsat satellite images (5TM of 1984, 7ETM + of 2000 and 8OLI of 2016), enhanced by field missions. Satellite images were processed using ENVI and ArcGIS software. Interview, focus group discussion methods and participatory mapping were used to identify the activities carried out by the local population. An inventory design consisting of four transects was used to measure dendrometric parameters and to identify land-use types. An estimation of carbon stocks in aboveground and underground woody biomass was made using allometric models based on non-destructive method. Dynamic of land-cover showed that the average annual rate of deforestation is 0.48%. The main activities at the base of this change are agriculture, house built-up and logging. Seven types of land-use were identified; adult secondary forests (64.10%), young secondary forests (7.54%), wetlands (7.39%), fallows (3.63%), savannahs (9.59%), cocoa farms (4.28%) and mixed crop farms (3.47%). Adult secondary forests had the highest amount of carbon ($250.75\;t\;C\;ha^{-1}$). This value has decreased by more than 60% for mixed crop farms ($94.67\;t\;C\;ha^{-1}$), showing the impact of agricultural activities on both forest cover and carbon stocks. Agroforestry systems that allow conservation and introduction of woody species should be encouraged as part of a participatory management strategy of this forest.

• Journal of Forest and Environmental Science
• /
• v.37 no.4
• /
• pp.292-303
• /
• 2021

Studies that relate the structure of tropical regrowth vegetation to soil properties are generally lacking in the literature. This study proposes three indices for assessing the structural regeneration of secondary forests. They are: (1) the tree diameter class, (2) the plant life form and (3) the woody/herbaceous plants ratio indices. They were applied to assess the regeneration status of forest regrowth vegetation (aged 1-10 years), derived savanna regrowth vegetation in south western Nigeria, and to secondary forests in different stages of succession in Columbia and Venezuela, Bolivia, Mexico in South and Central America and semi-arid savanna in Ethiopia and seasonal deciduous forest successional stages in India. In all the cases, the indices increased with increasing age of regrowth vegetation and hence, with increasing structural complexity of regenerating vegetation. The tree diameter class index increased from 32.1% in a 9-year secondary forest to 69.0% in an 80-year-old secondary forest in Columbia and Venezuela and from 0.4% in a 1-year fallow to 20.9% in 10-year regrowth vegetation in southwestern Nigeria. In semi-arid savanna in northern Ethiopia, the woody/herbaceous plants ratio index increased from 18.1% in a 5-year protected grazing enclosure to 75.1% in 15-year protected enclosure, relative to the status of 20-year enclosure. The indices generally had correlations of 0.6-0.90 with species richness and Simpson's/Margalef's species diversity, implying that they are appropriate measures of ecosystem development over time. The proposed indices also had strong and positive correlations with soil organic carbon and nutrients. They are therefore, significant indicators of fertility status.