• 한국자원식물학회지
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• 제12권1호
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• pp.69-79
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• 1999

1996년 4월1일부터 1998년 3월 31일까지 지리산의 국립공원에서 많은 고등균류를 채집하였다. 그것들을 생태적 자원면에서 분류한 결과는 다음과 같다. 희귀 균류는 진균문에서는 줄솔밭버섯(Omphalina ericetorum)을 포함하여 15종이고 변형균문에서는 아재비털먼지(Trichia affinis), 청황색 자루먼지(Physarum viride var. aurantium)을 2종을 확인하였다. 그리고 식용가능한 균류는 135종, 재배 가능한 버섯은 16종, 독버섯은 35종, 항암버섯(약용포함)은 68종, 균근형성균은 51종, 목재부후균(낙엽분해균포함)은 177종이었다.

• 한국균학회지
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• 제42권1호
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• pp.40-49
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• 2014

우리나라 산림에서 수집한 목재부후성 버섯 균주들에 대한 배지 및 온도별 균사생장 특성을 고체평판배지 상에서 조사하였다. 대부분의 균주들은 malt extract agar (MEA)나 sabouraud dextrose agar (SDA)보다는 PDA (potato dextrose agar) 배지에서 균사 생장력이 더 높았다. 몇몇 균주들을 제외하고, 균주들의 대부분은 PDA 배지 상에서 배양 시 $25^{\circ}C$에서 가장 잘 생장하였으며, 고온($30^{\circ}C$)보다는 저온($10^{\circ}C$)에서 균사 생장이 저조하였다. 2종류의 서로 다른 세포외효소 활성에 각각 특이적으로 반응할 수 있는 기질이 함유된 고체평판배지 상에서 모든 시험균은 carboxymethyl cellulase 활성과 laccase 활성을 나타냈다.

• 한국가구학회지
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• 제19권6호
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• pp.498-506
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• 2008

This study explored interpretation of the symbolical and metaphorical aesthetics in experiencing art/craft/handmade/studio furniture theoretically for 'wood culture experience class' or 'wood love experience class' by the Korea Forest Service because the class tends to focus on making simple wood works involved just in the practical part. Qualitative conceptual analysis as the principal methodology was used to achieve the theoretical context in the experience programs. After the lesson one on defining the postmodern aesthetics in New Design furniture as metaphor in the previous paper, I developed lesson two for an aesthetics of art and everyday life with wood furniture which expresses the metaphorical and symbolical aesthetics appeared as New Design furniture in postmodernism. As statement of objective in the second lesson, I explored that students in elementary schools personally transform and interpret the postmodern aesthetics of New Design furniture metaphorically with the meanings of symbol. As a result, the students will be able to write about the aesthetics of New Design furniture as metaphor in interpretation transformed by his/her perspective of a piece of selected New Design furniture.

• Mycobiology
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• 제36권4호
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• pp.270-273
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• 2008

Trichoderma spp. cause large crop losses of the cultivated shiitake mushroom, Lentinula edodes. We bred several shiitake strains that are resistant to Trichoderma spp. using di-mon mating to establish a useful method for controlling the greenmold disease. We examined the competitive ability of L. edodes against Trichoderma spp. using a dual culture system to select resistant strains. By screening Trichoderma-resistant strains, we found that among 11 parental strains, 4 strains, including KFRI 36, were confirmed resistant strains. They showed especially strong resistance to T. harzianum, which formed deadlock after mycelial contact and then invaded into the territory of T. harzianum. KFRI 171 also showed resistance to T. atroviride strains. Among 13 strains, which were made by hybridization of shiitake strains, 5 were confirmed to be resistant to Trichoderma, including KFRI 58-1. Their resistance was not correlated to the resistant activity of their parents’ strains. Two strains lose resistance and two strains acquire resistance compared to their parents’ strains. In SEM observation, the mycelium of L. edodes at the interaction zone of Lentinula-Trichoderma was rugged and swollen by T. harzianum.

• Journal of the Korean Wood Science and Technology
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• 제28권4호
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• pp.29-40
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• 2000

Highly purified Cerrena unicolor laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) caused the demethoxylation of milled wood lignin and several lignin related substances. The constitutive form of the enzyme produced extracellularly by C. unicolor fermenter culture was isolated and purified by ion-exchange chromatography on the DEAE-Toyopearl column and by affinity chromatography on a ConA-Sepharose and Syringyl-AH-Sepharose 4B columns. The enzyme was further immobilized on functionalized porous glass (CPG) and keratin coated CPG. The demethylating activity was monitored both by estimation of released methanol and by detection of the level of methoxyl groups (also in some water miscible solvents) after incubation of lignin materials with laccase preparations (free and immobilized). The effects of the incubation time and temperature on the demethoxylating activity of immobilized laccase preparations were also studied.

• 미생물학회지
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• 제48권1호
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• pp.37-41
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• 2012

본 연구에서는 sesquiterpene류의 생물학적 변환을 위한 생촉매로서 목재부후균을 선발하고 생물변환 가능성을 확인하였다. Sesquiterpene류 합성 전구체로 알려진 trans,trans-farnesol을 기질로 하여 총 19종의 목재부후균에 대해여 저항성 실험을 실시하였다. 기질의 농도를 달리하여 저항성 테스트를 실시하여 생물변환 가능성을 갖는 5종의 균주를 선발하였으며 선정된 균주를 이용하여 액체배지 조건에서 trans,trans-farnesol의 생물변환을 시도하였다. GC/MS를 이용하여 분석한 결과 Laetiporus sulphureus var. miniatus (jungh) Imaz, Coriolus versicolor (L. Fr). Prlar, Fomitopsis pinicola, Lampteromyces japonicas 등 4개의 균주에서 각각 nerolidol, eicosane, isoborneol, isocyclocitral 등의 대사산물을 확인할 수 있었다. 이와 같은 결과로 sesquiterpene류의 생물학적 변환을 위한 생촉매로서 목재부후균의 가능성을 확인하였다.

• Journal of the Korean Wood Science and Technology
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• 제49권6호
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• pp.591-601
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• 2021

Recently it is trend to increase wood use as carbon neutral materials, there is recognized to need necessarily durability improvement of wooden building. It is very rare to report existing on the identification of isolates causing discoloration in domestic weathered wooden building used for long period. The objective of this study was identification of fungi that cause discoloration on the exteriors of weathered domestic wooden buildings in the southern part of South Korea. Our findings can be helpful to establish protection technology for weather deterioration of domestic wooden buildings. Wood chip samples presumed to be contaminated with sapstain fungi were collected from the surface of wooden members used in wooden buildings at Jangheung, Jeollanam-do (two locations, #13 and #14), and Jeju Island (two locations, #31 and #33). The growth of microorganisms was confirmed by performing culture tests for the collected samples, and fungi were isolated, purified, and identified. The results indicated that the fungal strains isolated from wooden buildings #13 and #14 at Jangheung, Jeollanam-do, were 99.83% and 100% homologous to Aureobasidium melanogenum, respectively. For wooden building #31 at Jeju Island (two locations), the fungal strain isolated was 100% homologous to A. melanogenum, which is the same species isolated from the wooden buildings at Jangheung. The fungal strain isolated from wooden building #33 (Jeju Island) had 99.83% homology with A. pullulans, which is commonly found in wood degraded by weather or ultraviolet rays. Our findings can be utilized as a basis for establishing protection technology in domestic wooden buildings.

• 한국균학회지
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• 제36권1호
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• pp.45-50
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• 2008

잣버섯 균사체의 액체배양을 촉진하기 위해 소나무 목분의 첨가배양 조건을 구명하고자 하였다. 소나무의 여러 부위 중 목부의 목분에서 균사체 생장이 양호한 것으로 조사되었다. 목분을 액체 배양에 첨가하기 위한 전처리로서는 알카리 처리 조건에서 균사체 생장이 우수하였으나, 전처리를 하지 않아도 균사체 배양 촉진에 효과가 있는 것으로 나타났다. 액체배양 배지에 적정한 목분의 분말도 $200\;{\mu}m$ 이하로 낮을수록, 목분의 첨가 농도는 0.5%(w/v)로 또한 배지내의 탄소원인 glucose 농도는 10 g/l로 하는 조건에서 균사체 생장 촉진 효과가 큰 것으로 나타났다. 이러한 배양 조건에서 표고버섯 균사체를 배양한 결과도 균사배양 촉진 결과가 우수하여 액체배지에 목분 첨가에 의한 다른 목재부후균의 균사체 배양 촉진에도 효과가 있을 것으로 추정된다.

• Journal of the Korean Wood Science and Technology
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• 제34권1호
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• pp.68-78
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• 2006

돈분뇨 분해 장치의 발효 속도를 가속화시키는 토착미생물의 확보를 위하여 우리나라 각 지역에서 수집한 균주 중 우수한 균주를 선별하고 168 rDNA 증폭에 의한 동정을 실시하여 돈분뇨 분해에 관여하는 균주의 특성을 확인하기 위해 본 연구에서는 총 23장소(서울, 충청, 강원, 전북, 전념, 경남 지역)에서 28종류의 부숙 퇴비를 수집하여 생균수를 조사한 결과 대체적으로 $1{\times}10^5{\sim}10^8$의 분포 밀도를 보였다. 분리한 균주의 효소 생산 역가결과는 $30^{\circ}C$에서 보다 $55^{\circ}C$에서 배양된 고온호기성박테리아가 상대적으로 높은 섬유소분해효소와 전분분해효소 생산력을 보였고, genomic DNA의 추출에 의한 168 primer로 증폭하여 염기서열을 결정한 결과 돈분뇨 발효에 관여하는 고온호기성박테리아는 15종으로 동정되었다. 그 중 미생물제재를 제조하기 위하여 효소 역가 면에서도 우수성을 보이고 내생 포자를 형성하는 균주는 Bacillus subtihs로 확인되었다.

• Journal of the Korean Wood Science and Technology
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• 제26권1호
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• pp.19-28
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• 1998

For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows: 1. When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was $25^{\circ}C$, and $22.5\sim27.5^{\circ}C$ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106mg dry mycelia per 40ml media after 17 days. 2. In 1l-spawn bottle, growth of mycelium by inoculation of 20ml-liquid spawns were faster than 6g-sawdust spawn in spread of mycelia. On 2kg-bag culture, inoculations of 10ml-, 20ml- and 30ml-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in ampunt of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2kg-bag culture were 580g in 30ml-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20ml-liquid spawn and 10ml-liquid spawn, respectively. Thus, 30ml-liquid spawn inoculation was highest in yield.