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Identification by 16S rDNA Partial Sequencing of Thermophilic Bacteria with Fermentation of Pig Manure  

Kim, Myung Kil (Dept. of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Choi, Don Ha (Dept. of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Choi, In Gyu (Dept. of Forest science, College of Agriculture & Life Sciences, Seoul National University)
Kim, Byung Gyu (Korean Agricultural Culture Collection, National Institute of Agricultual Biotechnology)
Song, Jae Gyung (Korean Agricultural Culture Collection, National Institute of Agricultual Biotechnology)
Publication Information
Journal of the Korean Wood Science and Technology / v.34, no.1, 2006 , pp. 68-78 More about this Journal
Abstract
The purpose of this study was to estimate the identification of thermophilic hacterial with fermentation of pig manure. To identify the characters of thermophilic bacteria related to fermentation at a high temperature condition, we selected 28 different kinds of original settling thermophilic bacteria that were sampled at different 23 areas. They were distributed 1$1{\times}10^5{\sim}10^8CFU$ at medium and the enzyme activity at $55^{\circ}C$ incubation condition, especially cellulase and a-amylase, were higher than those of $30^{\circ}C$. Partial sequencing data for 165 rDNA region were obtained from 28 samples representing 15 different genera. Bacillus subcilis, one of those bacteria, has endodermic spores at high fermented condition.
Keywords
Fermentation; 16S rDNA; partial sequence; thermophilic bacteria; pig manure;
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