• Title/Summary/Keyword: wood culture

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The Change of Ultrasonic Transmission Velocity by Wood Decay

  • Hwang, Won-Joung;Lee, Hyun-Mi;Park, Young-Ran;Lee, Dong-Heub
    • Journal of the Korean Wood Science and Technology
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    • v.42 no.2
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    • pp.214-221
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    • 2014
  • The deterioration in wood by the brown-rot fungus (Fomitopsispalustris) and the white-rot fungus (Trametesversicolor) were measured using ultrasonic velocity. Those were used for the decay exposure and 4 wood species of wood as the test specimens, Pinusdensiflora, Larixkaempferi, Pinuskoraiensis and Pinusrigida, were chosen with both the brown- and white-rot culture petridish during 12 weeks. After 12 weeks, the decrease rate of ultrasonic velocity was measured at 10~15%. In both brown- and white-rot exposure experiments, P. rigida showed significant decrease in ultrasonic velocity (20%), L. kaempferi on the other hand did not show decrease in ultrasonic velocity. After the fungal exposure experiment, the inside of specimens was investigated by computer tomography (C/T). After C/T investigation, bending tests were performed.

The Laccase Activity of Trametes versicolor during Cultivation on Acetylated Wood and 13C-CP/MAS NMR Study (아세틸화 처리 목재에 배양시킨 Trametes versicolor의 Laccase활성과 13C-CP/MAS NMR 분석)

  • Son, Dong-Won;Lee, Dong-Heub
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.4
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    • pp.60-66
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    • 2001
  • For examine anti-degradation factors of acetylated wood, acetylated wood was incubated on Trametes versicolor. The laccase activity was examined in broth culture and solid fermentation that contain acetylated chips. The change of acetyl groups and chemical composition in the acetylated wood having massloss analysed by $^{13}C$-CP/MAS NMR. The laccase activity was detected in broth culture. When the T. versicolor contact to acetylated wood directly, the laccase activity was very low and couldn't maintain during test periods. Through the analysing of $^{13}C$-CP/MAS NMR, the acetylation took place carbohydrates as well as lignin and hydroxyl group of amorphous region was more easily substituted that of crystalline region The spectral analyses of $^{13}C$-CP/MAS NMR were shown that introduced acetyl bond was stable against fungal attack.

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Enzyme Activities and Cellulose Degradation of Domestic Softwoods in Shaking Culture of Fomitopsis palustris (국내산 침엽수 목분의 진탕배양에서 나타난 Fomitopsis palustris의 효소 활성 및 셀룰로오스 분해)

  • Choi, Doo-Yeol;Lee, Young-Min;Kim, Young-kyoon;Yoon, Jeong-Jun;Kim, Yeong-Suk
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.6
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    • pp.91-99
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    • 2007
  • Activities of the extracellular enzyme from Fomitopsis palustris, a brown-rot fungi, and by which crystallinity changes of cellulose in the various softwoods, such as Larix leptolepsis, Finns rigida, Finns koraiensis and Finns densiflora by liquid culture, were investigated. Activity of Cellobiohydrolase (CBH) from F. palustris was detected in the every test softwoods culture, showing activities of the Endoglucanase (EG), $\beta$-glucosidase (BGL) and $\beta$-1,4-xylosidase (BXL). It was shown high enzyme activities in the sapwood culture than heartwood of the same wood species, However, the enzyme activities in most of test wood cultures increased with longer incubation time, indicating a possibility of intermix sapwood and heartwood for degradation process by enzyme. Also it was shown that protein patterns of the extracellular enzyme from F. palustris in wood particle substrate of the several domestic softwoods were similar with each other wood species, which suggested the possibility of mixing all softwoods in saccharification by enzyme from F. palustris. Crystallinity reduction value of cellulose by F. palustris was 4.2~20.4% in 4 weeks cultivation, 12.9~28.9% in 8 weeks.

Screening and Evaluation of Streptomyces Species as a Potential Biocontrol Agent against a Wood Decay Fungus, Gloeophyllum trabeum

  • Jung, Su Jung;Kim, Nam Kyu;Lee, Dong-Hyeon;Hong, Soon Il;Lee, Jong Kyu
    • Mycobiology
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    • v.46 no.2
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    • pp.138-146
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    • 2018
  • Two-hundred and fifty-five strains of actinomycetes isolated from soil samples were screened for their antagonistic activities against four well-known wood decay fungi (WDF), including a brown rot fungus, Gloeophyllum trabeum and three white rot fungi Donkioporia expansa, Trametes versicolor, and Schizophyllum commune. A dual culture assay using culture media supplemented with heated or unheated culture filtrates of selected bacterial strains was used for the detection of their antimicrobial activity against four WDF. It was shown that Streptomyces atratus, S. tsukiyonensis, and Streptomyces sp. greatly inhibited the mycelial growth of the WDF tested compared with the control. To evaluate the biocontrol efficacy of S. atratus, S. tsukiyonensis, and Streptomyces sp., wood blocks of Pinus densiflora inoculated with three selected Streptomyces isolates were tested for weight loss, compression strength (perpendicular or parallel to the grain), bending strength, and chemical component changes. Of these three isolates used, Streptomyces sp. exhibited higher inhibitory activity against WDF, especially G. trabeum, as observed in mechanical and chemical change analyses. Scanning electron microscopy showed that cell walls of the wood block treated with Streptomyces strains were thicker and collapsed to a lesser extent than those of the non-treated control. Taken together, our findings indicate that Streptomyces sp. exhibits the potential to be used as a biocontrol agent for wood decay brown rot fungus that causes severe damage to coniferous woods.

Study for Encyclopedia of Furniture Ⅰ (실용가구용어사전 편찬을 위한 연구 1)

  • Moon, Sun-Ok;Choi, Sook-Kyung;Chun, Su-Kyung;Kang, Ho-Yang;Han, Gyu-Seong
    • Journal of the Korea Furniture Society
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    • v.21 no.4
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    • pp.338-346
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    • 2010
  • This study intends to gather terminology as a first step for encyclopedia of furniture from four areas such as traditional Korean furniture and architecture, modern and contemporary furniture, wood science, and wood culture by each expert. Since China, Japan, America, and European countries have chronologically influenced domestic furniture culture, the furniture terms are currently used by mixed languages such as Chinese characters, Japanese, English, Russian, Korean, etc., in the furniture fields and education. Domestic furniture companies and schools have had lots of difficulties in carrying out works due to the furniture terms not unified. This paper carried out through domestic furniture fields, internet, and furniture books from the inside and outside of the country, including the references of architecture, interior, crafts, and wood science and culture in order to research the terminology, results in the confusing language use of furniture terms at the furniture companies, schools for furniture education, and wood workshops having their own furniture terms. Therefore, for unified and standard furniture terminology, the terms gathered must be reviewed and conformed through the committee composed with the relevant experts so that it will be able to help the relevant business and education carry out their work better.

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Optimization of medium components and incubation time for the production of Paecilomyces tenuipes mycelia in submerged culture

  • Ha, Si Young;Jung, Ji Young;Lee, Dong Hwan;Yang, Jae-Kyung
    • Journal of Mushroom
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    • v.19 no.1
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    • pp.1-8
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    • 2021
  • The choice of the culture medium is an important factor for the mass production of mycelia in submerged cultures. The influence of liquid medium on the mycelial dry weight of Paecilomyces tenuipes was investigated in this study. The regression equation is expressed as Y=-1292.94187+17.78612X1+18.92425X2+2.11464X3-0.019375X1X2-0.006276X1X3+0.008177X2X3-0.070169X12-0.292175X22-0.008818X32, where Y represents the value of the mycelial dry weight (g/L), X1 is the particle size of wood sawdust in liquid medium (mesh), X2 is the concentration of the wood sawdust in liquid medium, and X3 is incubation time (h). The medium was optimized using a response surface methodology, and the optimal medium contained 30 g of wood sawdust (140 mesh), 20 g of glucose, and 10 g/L of peptone. Under these conditions, the mycelial dry weight reached 38.1 g/L (actual value). The culture medium containing wood sawdust is simple and easy to use, highly efficient, and eco-friendly, and its effectiveness in large preparations of P. tenuipes mycelia with low material costs has been demonstrated.

Determining the Wood (Parashorea spp.) Decaying and Metal Corroding Abilities of Eight Fungi

  • SUPRAPTI, Sihati;DJARWANTO, DJARWANTO;DEWI, Listya Mustika
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.1
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    • pp.50-60
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    • 2020
  • Fungi are wood-decaying organisms, and this is an important trait that should be considered in wood utilization. When fungi attack wood, it decreases the quality of the wood. The use of metal screws has become an important part of woodworking. The ability of fungi to decay wood and damage metal screws that are embedded into wood is varied. In this study, eight fungal species were evaluated with respect to their ability to decay Parashorea smythiesii and P. tomentella wood. In addition, the effect of fungi on corroding metal screws was determined using the Kolle flask method. The evaluation showed that the fungal species Schizophyllum commune, Pycnoporus sanguineus, and Polyporus arcularius were highly capable of decaying Parashorea spp. woods. The greatest wood weight loss occurred with the heartwood of P. tomentella exposed to S. commune. Based on the classification of wood resistance against fungal attack, the two Parashorea spp. were classified as moderately resistant woods (class III). Schizophyllum commune was classified as highly capable of decaying wood that was embedded with metal screws and was highly capable of corroding metal screws placed in fungi-culture media. The greatest weight of rust powder formed because of screw corrosion was obtained from screw-embedded wood exposed to S. commune. Additionally, the most severe corrosion of metal screws that were embedded into woods was caused by the activities of P. arcularius. Moreover, the average weight loss of screw-embedded wood was greater than that of unscrewed wood.

Degradation of Pentachlorophenol by Lignin Degrading Fungi and Their Laccases

  • Cho, Nam-Seok;Cho, Hee-Yeon;Pham, Hop Thi Bich
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.5 s.133
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    • pp.76-85
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    • 2005
  • The degradation of pentachlorophenol (PCP) by lignin degrading fungi was performed. Several fungi, Abortiporus biennis, Cerrena unicolor and Trametes versicolor, were tested to evaluate the inhibitory effect of PCP on their growth. At the extremal concentration of PCP $(500\;{\mu}M)$, only C. unicolor showed relatively fast growth (60% within 14 days) in the comparison to the control culture. In the case of A. biennis and C. unicolor, when initial PCP concentration was $50\;{\mu}M$, about 88.2% and 79.5% of PCP degradation were achieved within 3 days, respectively. When 2,5-xylidine (0.2 mM) was added to the C. unicolor culture, as high as 98% of PCP degradation was achieved within just an hour after its addition. A. biennis removed 44% of PCP at the same condition. PCP was completely disappeared when laccase activities reached to maximum.

Image Support and Wood Identification of Wood Crafts (I) - Focusing on Wooden Containers - (목공예품의 이미지제공 및 수종분석(I) - 목기류를 중심으로 -)

  • Kim, Sa-Ick
    • Journal of the Korea Furniture Society
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    • v.25 no.3
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    • pp.198-204
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    • 2014
  • Aggravation of natural environment, improvements in income, internet diffusion, spread of five-day work a week, and population aging seem very different from each other. However they boil down to consumption. These culture patterns are changing into environment-friendly and productive housing culture. In other words, people might tend to have frequent contacts with not only environment-friendly housing in bustling cities but also woods that is environment friendly materials in their leisure time. In the period of people young and old alike are in favor of woodcrafts, this study was conducted for giving information to who speicalized in woodcrafts. Furthermore, the study shows that other countries also added grain and figure to woodcrafts for visually appealing.

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Utilization of Robinia pseudoacacia as Sawdust Medium for Cultivation of Edible and Medicinal Mushrooms

  • Chai, Jung-Ki;Lee, Sung-Jin;Kim, Yoon-Soo
    • Plant Resources
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    • v.2 no.1
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    • pp.42-48
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    • 1999
  • This study was undertaken to examine the feasibility of black locust (Robinia pseudoacacia) as substrates for several edible mushrooms. For the cultivation of several edible and/or medicinal fungi on black locust, optimum bulk densities, synthetic or semisynthetic additives, natural additives and pretreatment methods were investigated. Fruit body yields of the fungi on various sawdust media composed of different wood species were also analyzed for testing the capability of black locust as a substrate for mushroom production. Mycelial growths decreased proportional when the bulk density increased. The most suitable carbon and nitrogen sources as additives to promote the mycelial growth were sucrose (2%, w/w) and ammonium phosphate (0.2%, w/w) respectively. When corn-powder and beer-waste as natural additives were added to sawdust of black locust showed the significant growth of mycelia. And the optimum mixing ratio was 10:2:1 (sawdust: corn-powder: beer-waste, w/w). Black locust after cold water treatment showed the outstanding mycelial growths. Any significant changes of pH, moisture content (%) and dry-weight losses (%) could not be found among culture substrates (sawdust of black locust, oak and poplar wood) examined before and after harvesting of fruit bodies. Yield of fruit bodies on black locust culture media were comparable with those culture media composed with oak and poplar wood. The present work indicated strongly the potentiality of black locust as raw materials for edible and medicinal mushrooms.

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