• Applied Biological Chemistry
• /
• v.27
• /
• pp.29-46
• /
• 1984

To utilize several species of hard wood as raw materials of feed products, fermentation characteristics of cellulosic substrates to single cell protein was investigated, and results were summarized as follows. Among the microorganisms investigated, Tricoderma viride was selected as one of the most cellulolytic. Mixed culture of fungi did not show a synergistic effect on cellulose degradation. When the fungi were cultured at $28^{\circ}C$ for 7 days in a medium containing wheat bran 25 g, cellulose 0.25 g, proteose peptone 0.025 g and tween 800.025 g, cellulotic activities on carboxy methyl cellulose and filter paper reached maximum at 12 hr. The alkali treatment resulted in increased degradation of substrate from 13 to 18% when treated with enzymes for 12h, and reducing sugar formation increased with decreased size of substrates. Glucose was a very good feedback inhibitor of the enzyme from T.viride than that of xylose. When the substrate was rehydrolyzed, hydrolysis rate was 31% to reducing sugars within 12 hr. Quantative anlysis with HPLC showed the ratio of glucose to xylose in sugar syrups as 1.77 to 1. For the purpose of producing cellulosic-single cell protein from the sawdust of mulberry tree, 15 strains of xylose-assimilating yeast were isolated from 42 samples of rotten woods and compost soils and examined for their ability to utilize xylose. Then three strains were selected by their strong xylose-assimilating activities. The cultivative condition, the growth characteristics, and protein and nucleic acid productivities of three strains were investigated. The results obtained were, 1. Wood hydrolysate of mulberry tree was assimilated by 5 strains of CHS-2, CHS-3, ST-40, CHS-12 and CHS-13. 2. The optimum initial pH and temperature for the growth of strain CHS-13 were 4.4 and $30^{\circ}C$. 3. The specific growth rate of strain CHS-13 was $0.23h^{-1}$ and generation time was 3.01 hrs at the optimum condition. 4. CHS-13 strain assimilated 81 % of sugar in wood hydrolysate. 5. CHS-13 strain was identified as Candida guilliermondii var. guilliermondii 6. When the CHS-13 strain was cultured in the wood hydrolysate containing yeast extract, L-protein content was increased with yeast extract concentration. 7. The L-protein and nucleic acid yields from wood hydrolysate were 0.73 mg/ml and $4.92{\times}10^{-2}\;mg/ml$ respectively. 8. An optimal nucleic acid content of CHS-13 strain was observed in the medium containing 0.2% of yeast extract.

• 보존과학연구
• /
• s.35
• /
• pp.111-119
• /
• 2014

Microorganisms were isolated from Dongchundang(wooden cultural heritage) with PDA medium culture. Nineteen species shows the cellulolytic activity. Methylobacterium sp. was the most active in cellulose degradation. The growth curve and pH were measured during incubation of the microorganism for 72 hours. The pH was increased with the increasing of microbial growth. The degree of cellulose degradation was determined with the amount of reducing sugar by use of dinitrosalicylic acid (DNS) method. The amount of reducing sugar was decreased after 45 hours. As a results, It should suggested that wood component were deteriorated by Methylobacterium sp..

• Journal of Food Hygiene and Safety
• /
• v.20 no.3
• /
• pp.175-178
• /
• 2005

Clean Natural is a new disinfectant of which main components are propolis and wood vinegar from Quercus mongolica. To evauate the bacterial reverse mutation of Clean Watural, the in vitro Ames test using Salmonella typhimurium TA98, TA100, TA102, TA1535 and TA1537 were performed with clean natural at the concentrations 0, 5, 2.5, and 1.25 mg/ml/plate. Clean Natural was negative in Ames test with Salmonella typhymuyium with and without rat liver microsomal enzyme (S-9 fraction). These results indicate that Clean Watural does not cause bacterial reverse mutation.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.11
• /
• pp.1540-1548
• /
• 2012

Manganese peroxidase (MnP) was isolated from the culture filtrate of the wood log mushroom Stereum ostrea (S. ostrea), grown on Koroljova medium, and then purified by ammonium sulfate [70% (w/v)] fractionation, DEAE-cellulose anion exchange chromatography, and Sephadex G-100 column chromatography, with an attainment of 88.6-fold purification and the recovery of 22.8% of initial activity. According to SDS-PAGE the molecular mass of the MnP was 40 kDa. The optimal pH and temperature were found to be 4.5 and $35^{\circ}C$, respectively. The enzyme was stable even after exposure to a pH range of 4.5 to 6.0, and at temperatures of up to $35^{\circ}C$ at a pH of 4.5 for 1h. The $K_m$ and $V_{max}$ values for the substrate phenol red were found to be $8{\mu}m$ and 111.14 U/mg of protein, respectively. The MnP also oxidized other substrates such as guaiacol, DMP, and veratryl alcohol. Sodium azide, EDTA, SDS, $Cu^{2+}$, and $Fe^{2+}$, at 1-5 mM, strongly inhibited enzyme activity, whereas $Ca^{2+}$ and $Zn^{2+}$ increased enzyme activity. The participation of the purified enzyme in the decolorization of dyes suggests that S. ostrea manganese peroxidase could be effectively employed in textile industries.

• Journal of Environmental Health Sciences
• /
• v.31 no.5 s.86
• /
• pp.411-414
• /
• 2005

Clean Natural is a new disinfectant of which main components are propolis and wood vinegar from Quercus mongolica. The mutagenicity of Clean Natural was studied by a micronucleus test in male ICR mice. The maximally tolerated dose (MTI) of Clean Natural was determined to >2.0 g/kg body weight. Therefore, the doses adopted for the micronucleus test was 2.0 g/kg as a high dose, 1.0 g/kg as a medium and 0.5 g/kg as a low of dose, respectively. Each of group was consisted of three doses of Clean Natural, positive control 2 mg/kg of mitomycin C and negative control 20 ml/kg of saline. A slide preparation was made at 24 hours following administration. No significant induction of micronuclei was observed in any of the three doses of Clean Natural orally administered. No cytotoxicity such as inhibition of hemopoiesis was observed in any group of test agent as the rate of polychromatic erythrocytes to total erythrocytes was over 40%. These results indicate that Clean Natural is not capable of inducing micronuclei in vivo mouse cells and thus has no genotoxicity in micronucleus test.

• Korean Journal of Human Ecology
• /
• v.20 no.2
• /
• pp.487-503
• /
• 2011

The purpose of this study was to investigate residents' perceptions and attitudes towards Han-Ok based on a study of the characteristics and elements of Han-Ok that experts perceived. For the study, in-depth interviews were conducted and the data of 7 experts and 24 Han-Ok residents were analyzed. The results of the study were as follows. First, the characteristics of spatial arrangements are primarily emphasized and provide emotional stability for the residents. Placement on the ground, a suitable scale, appropriate openings and closures, hierarchical spatial system and a spatial relationship provide a feeling of comfort and protection. Second, the spaces and the materials of Han-Ok are considered the representative elements providing functionality and health. The close relationship between the inside and outside area makes the air naturally circulate and natural materials such as wood, soil, and paper have the function as natural ventilation, heat storage, and in the prevention of various illnesses. Third, residents in modern society put more of a value on experiences of natural environments that are usually impossible in the city. The simple and incomplete characteristics in using color, materials, and form, provide a culture of story telling and a leisurely lifestyle. Fourth, the cold and spatial inconvenience of Han-Ok are solved by partially using modernized equipment and material. The functionality of spatial systems in Han-Ok needs to be promoted in order to change the residents' perceptions of cold in Han-Ok.

• Korean Journal of Microbiology
• /
• v.45 no.1
• /
• pp.82-85
• /
• 2009

Endocrine disrupting chemicals (EDCs) disturb animal hormonal system even at very low concentrations, and finally give harmful effects to human through the food web. A white rot fungus Phlebia tremellosa isolated in Korea, was reported to have good degrading activity against the endocrine disrupting phthalates. However, this fungus has very low manganese peroxidase (MnP) activity under various culture conditions while laccase and lignin peroxidase activities were high. We have isolated an MnP cDNA from Trametes versicolor which was involved in the degradation of EDCs, and constructed an MnP expression vector to use in the genetic transformation of P. tremellosa in order to get higher MnP producing strains. Many transformants had integrated expression vector in their chromosomal DNAs, and showed increased MnP activity. One of two transformants showed increased degradation of 4 EDCs (70${\sim}$88%) than the wild type (30${\sim}$45% degradation rates), and showed twice better removal of estrogenic activities generated by the EDCs than the wild type.

• Journal of Mushroom
• /
• v.18 no.1
• /
• pp.72-78
• /
• 2020

During the monitoring of fungal pests in 2016 and 2017, Acrodontium crateriforme, Naganishia friedmannii, Pestalotiopsis trachicarpicola, Penicillium wollemiicola, and Trichoderma thailandicum were isolated from indoor air, mushroom flies (Phytosciara flavipes), and media materials in the cultivation houses of oak wood mushroom (Lentinula edodes) located in Seocheon, Jangheung, Buyeo, and Yeoju, Korea. These fungal species were identified based on their morphological characteristics after their growth on PDA and subsequent molecular analyses of the 26S rDNA, 28S rDNA, β-tubulin gene, and translation elongation factor 1-α gene using PCR amplification and nucleotide sequencing were performed. The results showed that these fungi were previously undocumented in Korea. This study reports descriptions of their taxonomical and known properties.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.18 no.1
• /
• pp.28-38
• /
• 2004

The Hun-Min-Jeong-Eum(訓民正音) has been created under the political purpose that is to unite politics and cultures through a philosophical harmony of the confucianism as well as the Buddhism under the periodical circumstance that includes the change of a dynasty and a revolution in culture. The creating work has been greatly affected by the theoretical divination, that is the confucian ideas of North-Song dynasty which has been highly elaborated in those days. The shape of the characters of the Hun-Min-Jeong-Eum(訓民正音) has been influenced by the ancient letters existing at the time (especially Ga-Rim-To 加臨多), it has been throughly readjusted and recreated based on the philosophy. The consonant uses the Three-Pillars(三才)(ㅇ, ㅁ, △) in the style of the five elements and each velar-lingual-labial-dental-guttural sounds (牙舌脣齒喉音) is applicable to five elements also a 'ㅇ' is equivalent to the heaven, a 'ㅁ' to the earth. a '△' to the man. The vowel uses the five elements of ㅗ(water). ㅏ(wood), ㅜ(fire), ㅓ(metal), ㆍ(five-soil), ─(ten-soil) in the style of the Three-Pillars(三才) of ㆍ, ㅡ, ㅣ and the first created letters(初出字), and the secondary created letters(再出字) each can be attached to the eight trigrams(八卦). The consonant has 17 letters and the vowel has 11 letters, therefore the total number of the letters is 28. and those are equivalent to the 28 constellation(二十八宿) of the astronomy. Contrary to the underlying principle that has been applied during the time of the creation of the letters, when the consonant and the vowel are used, the consonant refers to the heaven (天), and the vowel refers to the earth(地). a consonant take a part in making a point on the starting place of the pronounce, a vowel take a part in the keeping the energy to the end.

• Journal of Conservation Science
• /
• v.32 no.1
• /
• pp.63-73
• /
• 2016

This study defined material and characteristics of 24 glass fragments and 26 whole glass beads. The feature of glass beads shape are divided into 5 types following color, size, weathering condition and manufacturing techniques. Through the chemical composition, the first and second type is soda glass, the third type is potash glass, the fourth and fifth type is lead barium glass. This site showed the aspect that the chemical composition is changed according to the feature of glass shape and was found that various chemical compositions. Looking at the flow of glass culture, the tomb that are lead barium glass IItype and potash glass I, IItype is relatively preceding period and the tomb that are soda glass and lead barium glass IIItype is following period.