• The Korea Journal of Herbology
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• v.21 no.2
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• pp.181-188
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• 2006

Objectives : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix imported from China are circulated in Korea. Morphological identification was attempted by comparing external and internal forms of these materials. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : 1. Southern type of Osterici Radix has short rhizome and many small and long roots, while northern type of it is mostly composed of rhizomes with little small reds and sometimes looks like bamboo because of the extention of the parts between joints. 2. Parenchymal cell of southern type of Osterici Radix is filled with starch grain, while that of nothern type includes less starch grain with lamination crossed on its cell wall. 3. Surface of root and rhizome of wild Osterici Radix has some marks of fibrous root raised in the form of nodule, but cultivated Osterici Radix does not have this kind of marks. 4. Notopterigii Rhizoma et Radix is mostly composed of rhizomes, and its form is similar to that of a silkworm or joints of bamboo. Parenchymal cell is filled with starch grains, and lamination is crossed on its cell wall but not salient. Conclusion : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix circulated in Korea can be easily identified because of their differences in their external and internal forms.

• Journal of Sericultural and Entomological Science
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• v.53 no.1
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• pp.55-60
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• 2015

Melittin is the main component of Bee Venom and has antibacterial activity against several bacteria. To produce the melittin antimicrobial peptide, we constructed transgenic silkworm that expressed melittin gene under the control BmA3 promoter using piggyBac vector. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor vector and helper vector were micro-injected into 300 eggs of bivoltin silkworms, Baegokjam. In total, 131 larvae (G0) were hatched and allowed to develop into moths. The resulting G1 generation consisted of 36 broods, and we selected 4 broods containing at least 1 EGFP-positive embryo. The rate of successful transgenesis for the G1 broods was 11%. We identified 12 EGFP-positive G1 moths and these were backcrossed with wild-type moths. With the aim of identifying a melittin as antimicrobial peptide, we investigated the Radical diffusion Assay (RDA) and then demonstrated that melittin possesses high antibacterial activities against gramnegative bacteria.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.1
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• pp.1-4
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• 2011

This study was conducted to test the use of colored cocoon silkworm, Golden Silk, as a host for the fruiting body production of Cordyceps mushroom. Golden Silk variety had a shorter larval period than Yangwonjam at $25.3^{\circ}C$ and 78.5% RH: The average larval period of the Golden Silk was 24 days and 7 hr, and that of the Yangwonjam was 24 days and 15 hr. In the infection test of I. tenuipes on silkworm, the larval period of normal silkworms was longer than the silkworms inoculated with I. tenuipes in both Golden Silk and Yangwonjam. The pupae survival percentage of Golden Silk was 94.4%, whereas it was 91.9% in Yangwonjam. Golden Silk had a higher pupation rate than Yangwonjam. The pupation rate of normal silkworms was about 5% higher than that of silkworms inoculated with I. tenuipes. Infection rate of I. tenuipes into larvae of 5th instar newly exuviated silkworm was 93.8% in Golden Silk, and 96.9% in Yangwonjam. Synnemata production of I. tenuipes was better in Yangwonjam with an incidence rate of 99.8% than Golden Silk with 98.6%. The average synnemata weight of Golden Silk was 1.12 g and that of Yangwonjam 1.29 g. Golden Silk had a lower synnemata weight than Yangwonjam. But no significant difference was observed in synnemata weight varieties. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and similar in shape and color to wild synnemata collected in Korea.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2001.11a
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• pp.133-133
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• 2001

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2001.10a
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• pp.65-65
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• 2001

no Abstract, See Full Text

• International Journal of Industrial Entomology and Biomaterials
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• v.45 no.1
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• pp.17-21
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• 2022

The present study aimed to determine whether a hemolymph prepared from Antheraea yamamai larvae had the same biological activities using a Bombyx mori hemolymph prior to exposure to lipopolysaccharide (LPS) in order to induce an inflammatory response. The effects of the hemolymph were determined using a reverse transcription-quantitative polymerase chain reaction to assess the expression of pro-inflammatory molecules. The A. yamamai hemolymph exerted anti-inflammatory effects on LPS-activated human monocytic leukemia cells via Toll-like receptor (TLR) 4-mediated suppression, similar to the B. mori hemocyte extract. Treatment with the A. yamamai hemolymph significantly suppressed LPS-induced upregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression at all tested concentrations compared with the control, similar to the B. mori immune-challenged hemolymph. Finally, the A. yamamai hemolymph, like the B. mori immune-challenged hemolymph, suppressed all of these concentrations in a dose-independent manner. These results demonstrate that the hemolymph of A. yamamai exhibited important biologically active substances. Further in-depth functional studies are required to fully understand the mechanisms underlying the biological activities of wild-type silkworm hemolymphs.

• International Journal of Industrial Entomology and Biomaterials
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• v.19 no.1
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• pp.187-192
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• 2009

Silk filament progression study applying backcross breeding with recipient parent Jata and donor parent Daba ecoraces of tropical tasar silkworm, Antheraea mylitta Drury conducted during 2006 to 2008, revealed introgression of filament denier (10.2 d) superior to both parents at BC4 level. Also, the silk waste (0.35%) and filament breaks (2.6 nos) were reduced compared to both parents, while the filament length (973 m) and non break filament (296 m) improved over donor and could attain closer to recipient parent. The best performance of Jata $\times$ Daba at F1 level, with highest silk filament length (1646 m) of 12.5 d denier, denotes heterosis impact on silk trait with parental heterogenousity, an advantage to exploit silk filament yield. The progression of quality in terms of finer filament denier, less silk waste and least number of filament breaks over both parents and improvement in filament length and non break filament over donor parent except for a marginal shortage against recipient at BC4 level indicates the prospects of Jata and Daba ecoraces as source of breeding material for qualitative improvement of tasar silk filament. The study suggests that the commercially important finer denier of tasar silk filament can be attained with minor reduction in silk yield of wild Jata ecorace by adopting repeated backcrossing for four generations with semi domesticated Daba ecorace.

• Journal of Sericultural and Entomological Science
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• v.36 no.1
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• pp.30-36
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• 1994

choChorion(egg-shell) morphology of the F1 hybrid between Bombyx mori and Bombyx mandarina has been observed by scanning electrom microscope and chorion protein was analyzed by electrophoresis. The chorion surface structure of F1 hybrids in the lateral(flat) region was similar to that of maternal line. The F1 hybrids chorion was found to have basically a three layer structure. The middle and inner layer were very much like those of the Bombyx mandarina and Bombyx mori. There were many conic pillar structures in the outer layer of the F1 hybrid, which was similar to Bombyx mandarina. This conic pillar structure had a thin cover layer was more clear in the dorsal and ventral side of the F1 hybrid chorion. The conic pillar structure of Bombyx mandarina was found to be dominant in F1 hybrid chorion irrespective of their maternal line. Major components of chorion protein were analyzed by two-dimensional electrophoresis and found to have isoelectric points in the range of pH 4.0-6.5 and molecular weight 10 to 50 kd. F1 hybrid chorion protein components related directly to those of the maternal line. The conic pillar structure was dominat characteristic and it was present in all F1 hybrid.

• International Journal of Industrial Entomology and Biomaterials
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• v.43 no.1
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• pp.29-36
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• 2021

Silkworms have long been bred with human history to produce silk. It has been with humans for longer than other industrial insects, and the silkworm variety has been continuously improved. Silkworms have been developed into the optimal form for producing high quality silk and pupae. Recently, the production of transgenic silkworms has further expanded the possibility of industrial value of silkworms. Kynurenine 3-monooxygenase (KMO), which is a flavin enzyme, is known for its involvement in ommochrome pigment synthesis. In the field of mammals, including humans, previous studies have revealed the function and role of KMO, which is an important enzyme for various immune responses and cell protection. However, in the case of insects, the function of KMO has only been studied to be involved in the formation of pigment, and accordingly, KMO is used exclusively on screening for generation of transgenic insects as a marker. In this study, using KMO-edited silkworms, it was intended to discover the novel functions and roles of KMO in silkworms by identifying changes in the expression of various genes associated with stress and growth. The changes were observed in expressions of genes regulating on stresses to survive and those on ecdysteroid hormone between wild-type (WT) silkworms and kmo mutant silkworms. The loss of KMO, in particular, decreased the expression of the shadow (sad) gene, one of the Halloween genes in the synthesis of ecdysteroid. In conclusion, these results suggest that silkworm KMO is responsible for potential functions regarding stress response and ecdysteroid synthesis.

• Journal of Sericultural and Entomological Science
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• v.49 no.2
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• pp.47-50
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• 2007

Insect cell culture system has been demonstrated the effective means of producing medical and agricultural products. Furthermore, Fetal bovine serum (FBS) is in wide use in insect cell culture. Silkworm hemolymph was tested to develop as a substitute for FBS and was effective in insect cell growth. Hemolymph is oxidized and darkens visibly during the collection from silkworms due to the activity of tyrosinase in it. Toxic quinones are produced by the oxidation and consequently inhibit the cell growth. Heat treatment can be used to prevent the oxidation; however, the oxidation may occur during the collection of hemolymph before it is heat-treated. Hemolymphs collected from 257 different strains of silkworms were examined to select the slowly oxidized hemolymphs. Hemolymphs collected from mutant strains such as $Y_4$, TBO and $wE^b$ showed relatively slow color changes. Oxidation rates of the hemolymphs were measured by the absorbance change using a spectrophotometer. The absorbance of mutant hemolymph reached the saturation value at $20^{\circ}C$ in each 330 min ($Y_4$), 360 min (TBO) and 450 min ($wE^b$) min, whereas the total oxidation time of the wild-type (Baekokjam) hemolymph at the same temperature was 120 min. The cell growth in the medium supplemented with mutant species hemolmph was more effective that in the medium supplemented with Baekokjam species hemolymph.