International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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The changes of stresses and ecdysteroid biosynthesis gene expression levels in Kynurenine 3-monooxygenase mutant Bombyx mori

  • Jeong, Chan Young (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Lee, Chang Hoon (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kim, Su Bae (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kang, Sang Kuk (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Ju, Wan-Taek (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kim, Seong-Wan (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kim, Nam-Suk (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kim, Kee Young (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Park, Jong Woo (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
  • Received : 2021.08.06
  • Accepted : 2021.09.13
  • Published : 2021.09.30
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Abstract

Silkworms have long been bred with human history to produce silk. It has been with humans for longer than other industrial insects, and the silkworm variety has been continuously improved. Silkworms have been developed into the optimal form for producing high quality silk and pupae. Recently, the production of transgenic silkworms has further expanded the possibility of industrial value of silkworms. Kynurenine 3-monooxygenase (KMO), which is a flavin enzyme, is known for its involvement in ommochrome pigment synthesis. In the field of mammals, including humans, previous studies have revealed the function and role of KMO, which is an important enzyme for various immune responses and cell protection. However, in the case of insects, the function of KMO has only been studied to be involved in the formation of pigment, and accordingly, KMO is used exclusively on screening for generation of transgenic insects as a marker. In this study, using KMO-edited silkworms, it was intended to discover the novel functions and roles of KMO in silkworms by identifying changes in the expression of various genes associated with stress and growth. The changes were observed in expressions of genes regulating on stresses to survive and those on ecdysteroid hormone between wild-type (WT) silkworms and kmo mutant silkworms. The loss of KMO, in particular, decreased the expression of the shadow (sad) gene, one of the Halloween genes in the synthesis of ecdysteroid. In conclusion, these results suggest that silkworm KMO is responsible for potential functions regarding stress response and ecdysteroid synthesis.

Keywords

Acknowledgement

This study was supported by 2021 the RDA fellowship program of National Institute of Agricultural Science and with the support through a grant (No. PJ01270901) from the Rural Development Administration, Republic of Korea.

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