• Journal of Plant Biology
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• v.39 no.4
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• pp.273-278
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• 1996

Arabidopsis mutants deficient in flavonoid and sinapate ester (tt4 and fah1-7, respectively) were evaluated in vivo and in vitro to study the possible role of flavonoid compounds in pollen tube growth. In vivo, we investigated pollen tube growth in the pistils of the mutants and wild type(Ler). The growth of pollen tubes was significantly different among the three genotypes. In the fal1-7 pistils, the tubes grew to a greater length relative to those of the wild type or tt4. To examine in vitro pollen tube growth, a solid medium was devised for pollen germination and subsequent growth. In vitro, the identical result was obtained; fahl-7 pollens developed the longest tubes and elongated most rapidly. Therefore, the growth response of pollen tubes to phenolic compounds was examined by adding quercetin or sinapate ester in various concentrations to the media. Quercetin enhanced both germination rate and tube growth in the pollens of the mutants and the wild type, especially in tt4. In contrast, sinapate ester inhibits pollen germination and pollen tube growth in three genotypes. These results suggest that flavonoids and related phenolic compounds have physiological role in the plant reproductive system.

• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.1
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• pp.23-33
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• 2007

A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation($-196^{\circ}C$) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.612-616
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• 2006

In recent years, there has been increasing concerns in gene flow from GM crops to wild or weedy relatives as a potential risk in the commercialization of GM crops. To access the possibility of the environmental impacts by GM rice, small-scale experiments of gene transfer were carried out. Herbicide and drought stress resistant GM rice and non-GM rice Nakdongbyeo, wild rice Oryza nivara, and weedy rice Sharebyeo were used for artificial pollination experiments and bar gene was used as a tractable marker after pollination. The harvested putative hybrid seeds after artificial pollination were germinated and true hybrid plants were selected by basta treatment. The hybrid plants were verified again by PCR amplification of bar and trehalose-6-phosphate phosphatase (TPP) genes and RAPD PCR analysis.

• Korean Journal of Medicinal Crop Science
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• v.13 no.5
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• pp.262-269
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• 2005

The objective of this study was to assess genetic diversity among 6 different wild ginseng populations from New York, Kentucky, North Carolina, Pennsylvania, Tennessee and Virginia, and to compare these wild populations to one cultivated population. RAPD markers were used to estimate the genetic difference among samples from the 7 populations. The 64 random primers were screened, and 15 primers were selected which exhibited the 124 highly reproducible polymorphic markers. The ratio of discordant bands to total bands scored was used to estimate the genetic distance within and among populations. Multidimensional scaling (MDS) of the relation matrix showed distinctive separation between wild and cultivated populations. The MDS result was confirmed using pooled chi-square tests for fragment homogeneity. This study suggests that RAPD markers can be used as population-specific markers for American ginseng.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.10a
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• pp.56-56
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• 2017

• Proceedings of the Korean Society of Crop Science Conference
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• 2003.05a
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• pp.17-25
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• 2003

• Journal of Crop Science and Biotechnology
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• v.11 no.2
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• pp.111-118
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• 2008

Soybean is sensitive to waterlogging stress, leading to reduce their growth and yield significantly. The objective of this study was to characterize the relative sensitivities of biomass accumulation and specific nodule activity under waterlogging stress between supernoduating mutants, 'SS2-2' and 'Sakukei 4' and their wild-type soybeans, 'Sinpaldalkong 2' and 'Enrei', respectively. Flooding treatment was performed to soybean plants grown in a pot by waterlogging for 15 days from the beginning bloom(R1) stage under natural light. The nodule number and weight were considerably decreased by waterlogging stress. The bleeding sap rate of waterlogging soybean plants was decreased by 78-80% in supernodulating mutants and 65-74% in their wild types compared to control plants. The relative ureide-N content was also decreased by waterlogging and the reduction was high in supernodulating mutants. This may cause the massive reduction of shoot and root dry weight and leaf area in waterlogged soybean plants. There was a varietal difference in response to the waterlogging stress. During the waterlogging, supernodulating mutants maintained higher spad value than their wild types. Particularly, the difference between soybean varieties was clear in low rank leaves from the top. Also, supernodulating mutants showed a weak waterlogging tolerance than their wild types. Under waterlogging conditions, massive nodules were considerably destroyed and specific nodule activity after waterlogging may not be recovered when compared to their wild-type soybeans. Supernodulating mutants showed lower seed yield than their wild types in waterlogging conditions.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.53-53
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• 2020

본 실험은 다양한 생리활성이 보고되어 약용으로 널리 쓰이는 개똥쑥(Artemisia annua L.) 종자의 온도에 따른 발아 특성을 조사하기 위해 진행하였다. 미세온도구배 발아기를 이용하여 낮과 밤의 온도를 각각 5 ~ 35℃ 범위에서 낮과 밤의 시간을 12시간으로 고정하고, 낮 온도가 밤 온도보다 크거나 같은 조건을 설정하여 총 27개의 온도조건으로 개똥쑥 종자의 최종 발아율 및 발아율과의 관계를 분석하였다. 실험결과, 개똥쑥 종자는 실험에 사용한 모든 온도조건에서 발아가 가능한 것으로 나타났으며, 25/10℃(낮/밤) 조건에서 90%로 가장 높게 조사되었다. 또한, 발아율 조사결과를 통해 일평균온도뿐만 아니라 일교차온도도 발아율에 영향을 미치는 것으로 판단되어 일평균온도와 일교차온도로 나누어 발아율과의 관계를 분석하였다. 온도조건과 발아율과의 연속적인 발아특성을 분석하기 위해 다중회귀분석과 비선형 회귀분석을 이용하여 온도 조건과 상대적 발아율의 관계를 수식으로 표현하였다. 일평균온도를 기준으로 발아율과의 관계를 분석한 결과, 5 ~ 35℃의 모든 일평균 온도범위에서 유의성이 나타났으며 5, 7.5, 32.5, 35℃는 상대적인 음의 영향력을, 나머지 조건에서는 상대적인 양의 영향력을 가진 것으로 분석되었다. 일교차온도를 기준으로 발아율과의 관계를 분석한 결과, 0 ~ 25℃의 모든 일교차 온도범위에서 양의 영향력을 가진 것으로 분석되었다. 일평균온도는 19.3℃에 가까울수록, 일교차온도는 14.9℃에 가까울수록 발아에 대한 영향력이 큰 것으로 조사되었다. 각 수식을 통해 도출된 수치화된 온도에 따른 개똥쑥 종자의 일일누적 온도 영향력을 temperature score(TS)로 설정하였다. 본 연구에서 도출된 수식을 통해 누적 TS를 계산한 결과, 14.9 TS가 누적되었을 때 발아율이 85% 이상으로 나타날 것으로 예측되었다.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.204-204
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.26-26
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• 2022