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Improving the Viability of Freeze-dried Probiotics Using a Lysine-based Rehydration Mixture

  • Arellano, Karina;Park, Haryung;Kim, Bobae;Yeo, Subin;Jo, Hyunjoo;Kim, Jin-Hak;Ji, Yosep;Holzapfel, Wilhelm H.
    • Microbiology and Biotechnology Letters
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    • v.49 no.2
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    • pp.157-166
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    • 2021
  • The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

Assessment of deformations and internal forces in the suspension bridge under eccentric live loads: Analytical algorithm

  • Zhang, Wenming;Lu, Xiaofan;Chang, Jiaqi;Tian, Genmin;Xia, Lianfeng
    • Structural Engineering and Mechanics
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    • v.80 no.6
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    • pp.749-765
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    • 2021
  • Suspension bridges bear large eccentric live loads in rush hours when most vehicles travel in one direction on the left or right side of the bridge. With the increasing number and weight of vehicles and the girder widening, the eccentric live load effect on the bridge behavior, including bending and distortion of the main girder, gets more pronounced, even jeopardizing bridge safety. This study proposes an analytical algorithm based on multi-catenary theory for predicting the suspension bridge responses to eccentric live load via the nonlinear generalized reduced gradient method. A set of governing equations is derived to solve the following unknown values: the girder rigid-body displacement in the longitudinal direction; the horizontal projection lengths of main cable's segments; the parameters of catenary equations and horizontal forces of the side span cable segments and the leftmost segments of middle span cables; the suspender tensions and the bearing reactions. Then girder's responses, including rigid-body displacement in the longitudinal direction, deflections, and torsion angles; suspenders' responses, including the suspender tensions and the hanging point displacements; main cables' responses, including the horizontal forces of each segment; and the longitudinal displacement of the pylons' tower top under eccentric load can be calculated. The response of an exemplar suspension bridge with three spans of 168, 548, and 168 m is calculated by the proposed analytical method and the finite element method in two eccentric live load cases, and their results prove the former's feasibility. The nonuniform distribution of the live load in the lateral direction is shown to impose a greater threat to suspension bridge safety than that in the longitudinal direction, while some other specific features revealed by the proposed method are discussed in detail.

The EduCare Model Combining Online Care in Blended Learning (온라인 돌봄을 결합한 블렌디드 러닝 EduCare 모델)

  • Han, Jeonghye;Lee, Sihoon;Lee, Wonrae;Choi, Eunjung;Lee, Jayong
    • Journal of The Korean Association of Information Education
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    • v.26 no.3
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    • pp.167-174
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    • 2022
  • In blended learning, which combines school and online learning, unidirectionalness and disconnection of interaction are pointed out as the reason for widening the educational gap. This study conducted focus group interviews with teachers, students, and parents to develop a blended learning model to enhance the effectiveness of online learning. In order to increase the interaction between teachers and students online, the EduCare model considering care factors was proposed. Six practical models were proposed for the blended EduCare model. Not only interaction can be maximized, but learning reality and psychological well-being, social presence can be improved, and the effectiveness of online learning can be cultivated positively.

Percutaneous two unilateral iliosacral S1 screw fixation for pelvic ring injuries: a retrospective review of 38 patients

  • Son, Whee Sung;Cho, Jae-Woo;Kim, Nam-Ryeol;Cho, Jun-Min;Choi, Nak-Jun;Oh, Jong-Keon;Kim, HanJu
    • Journal of Trauma and Injury
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    • v.35 no.1
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    • pp.34-42
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    • 2022
  • Purpose: Percutaneous iliosacral (IS) screw fixation for pelvic ring injuries is a minimally invasive technique that reduces the amount of blood loss and shortens the procedure time. Moreover, two unilateral IS S1 screws exhibit superior stability to a single IS screw and are also safer for neurological injuries than an S2 screw. Therefore, this study aimed to evaluate fixation using percutaneous two unilateral IS S1 screws for pelvic ring injuries and its subsequent clinical outcomes. Methods: We retrospectively reviewed 38 patients who underwent percutaneous two unilateral IS S1 screw fixation for pelvic ring injuries. The procedure time, blood loss, achievement of bone union, radiological outcomes (Matta and Tornetta grade), and postoperative complications were evaluated. Results: The mean procedure time, hemoglobin loss, bone union rate, and time to union were 40.1 minutes (range, 18-102 minutes), 0.6 g/dL (range, 0.3-1.0 g/dL), 100%, and 153.2 days (range, 61-327 days), respectively. The Matta and Tornetta grades were excellent, good, and fair in 24 (63.1%), 11 (28.9%), and three patients (7.9%), respectively, and the postoperative complications were S1 screw loosening, widening of the symphysis pubis (2.3 and 2.5 mm), lumbosacral plexopathy, and S1 radiculopathy in one (2.6%), two (5.3%), one (2.6%), and one patient (2.6%), respectively. However, all neurological complications recovered spontaneously. Conclusions: Percutaneous two unilateral IS S1 screw fixation was useful for treating pelvic ring injuries. In particular, it involved a short procedure time with little blood loss and also led to 100% bone union and good radiological outcomes.

Analysis of Academic Achievement Data Using AI Cluster Algorithms (AI 군집 알고리즘을 활용한 학업 성취도 데이터 분석)

  • Koo, Dukhoi;Jung, Soyeong
    • Journal of The Korean Association of Information Education
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    • v.25 no.6
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    • pp.1005-1013
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    • 2021
  • With the prolonged COVID-19, the existing academic gap is widening. The purpose of this study is to provide homeroom teachers with a visual confirmation of the academic achievement gap in grades and classrooms through academic achievement analysis, and to use this to help them design lessons and explore ways to improve the academic achievement gap. The data of students' Korean and math diagnostic evaluation scores at the beginning of the school year were visualized as clusters using the K-means algorithm, and as a result, it was confirmed that a meaningful clusters were formed. In addition, through the results of the teacher interview, it was confirmed that this system was meaningful in improving the academic achievement gap, such as checking the learning level and academic achievement of students, and designing classes such as individual supplementary instruction and level-specific learning. This means that this academic achievement data analysis system helps to improve the academic gap. This study provides practical help to homeroom teachers in exploring ways to improve the academic gap in grades and classes, and is expected to ultimately contribute to improving the academic gap.

Does platelet-rich fibrin increase bone regeneration in mandibular third molar extraction sockets?

  • Azuka Raphael, Njokanma;Olawunmi Adedoyin, Fatusi;Olufemi Kolawole, Ogundipe;Olujide Olusesan, Arije;Ayodele Gbenga, Akomolafe;Olasunkanmi Funmilola, Kuye
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.48 no.6
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    • pp.371-381
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    • 2022
  • Objectives: This study determined the effect of platelet-rich fibrin (PRF) on extraction socket bone regeneration and assessed the patterns and determinants of bone regeneration after the surgical extraction of impacted mandibular third molars. Materials and Methods: This prospective study randomly allocated 90 patients into two treatment groups: A PRF group (intervention group) and a non-PRF group (control group). After surgical extractions, the PRF group had PRF placed in the extraction socket and the socket was sutured, while the socket was only sutured in the non-PRF group. At postoperative weeks 1, 4, 8, and 12, periapical radiographs were obtained and HLImage software was used to determine the region of newly formed bone (RNFB) and the pattern of bone formation. The determinants of bone regeneration were assessed. Statistical significance was set at P<0.05. Results: The percentage RNFB (RNFB%) was not significantly higher in the PRF group when compared with the non-PRF group at postoperative weeks 1, 4, 8, and 12 (P=0.188, 0.155, 0.132, and 0.219, respectively). Within the non-PRF group, the middle third consistently exhibited the highest bone formation while the least amount of bone formation was consistently observed in the cervical third. In the PRF group, the middle third had the highest bone formation, while bone formation at the apical third was smaller compared to the cervical third at the 8th week with this difference widening at the 12th week. The sex of the patient, type of impaction, and duration of surgery was significantly associated with percentage bone formation (P=0.041, 0.043, and 0.018, respectively). Conclusion: Placement of PRF in extraction sockets increased socket bone regeneration. However, this finding was not statistically significant. The patient's sex, type of impaction, and duration of surgery significantly influenced the percentage of bone formation.

A study on the change and cause of the channel in the midstream of singwang river and naeng river in pohang - Comparing the changes before and after typhoon hinnamnor with the past - (포항 신광천과 냉천 중류의 하도 변화와 그 원인에 대한 분석 - 과거 및 태풍 힌남노 전후 비교를 중심으로 -)

  • Seonggi An;Chanjoo Lee;Woo-Chul Kang;Jongmin Kim;Gyeonghyeon Min;Byungsun Yoo
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.61-61
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    • 2023
  • 우리나라는 여름철 집중호우 시 발생하는 홍수로 인해 중소하천의 확폭(Widening) 및 하안침식이 자주 발생하고 있으나, 이에 대해 홍수 피해 관점으로만 분석되고 있으며 체계적인 수리학적, 지형학적 분석은 미흡한 실정이다. 본 연구에서는 먼저, 1982-2022년의 지도 및 항공사진, 정사영상 등을 분석하여 포항시 신광천과 냉천 중류의 하도 변화를 살펴보고, 2022년 9월 태풍 힌남노의 내습 시 발생한 홍수로 인해 초래된 하도 변화를 분석하였다. 신광천 항사리 지점의 경우, 하천 우안에 제방이 축조되지 않았던 1982년에는 하폭이 상대적으로 넓었으나, 1992년 제방 축조 이후 우안 충적지가 하도에서 분리되며 하폭이 감소하였다. 2011년의 하도는 대부분의 면적이 식생으로 덮여 하천의 흐름이 잘 관찰되지 않으나, 2021년에 식생 제거로 하도가 식별되지만 하폭은 과거에 비해 더욱 감소하였다. 태풍 힌남노 내습 시 포항시의 6시간 누적 강우량은 315.3 mm 로 100년 빈도를 초과하는 집중호우가 발생하였으며, 그 결과 확폭이 발생하였다. 단면에서 보면 좌안 활주사면은 5 m 가량의 퇴적고를 보였고, 우안 공격사면은 평균 23.3 m 측방으로 침식되었다. 그 결과 1982년 하폭과 유사한 수준으로 확대되었다. 문덕리 지점(공장지대)과 냉천 용산리(아파트)의 경우에도 각각 평균 14 m, 18 m 의 하안침식을 보였다. 이러한 결과는 지난 30여 년 전 축조된 기존 제방이 유실되면서 발생한 것으로 홍수로 인해 과거의 하폭으로 복귀할 수 있음을 의미한다. 2022년 홍수에 의한 하도 변화와 같은 지형학적 변화를 고려할 때, 보다 안정적인 하천관리를 위해서는 하천 설계 시 과거 지도상의 하도 범위 및 기왕홍수로 인한 하안선 변동을 고려하는 것이 필요하다.

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A Study on the Protection of Biometric Information against Facial Recognition Technology

  • Min Woo Kim;Il Hwan Kim;Jaehyoun Kim;Jeong Ha Oh;Jinsook Chang;Sangdon Park
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.8
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    • pp.2124-2139
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    • 2023
  • In this article, the authors focus on the use of smart CCTV, a combnation of biometric recognition technology and AI algorithms. In fact, the advancements in relevant technologies brought a significant increase in the use of biometric information - fingerprint, retina, iris or facial recognition - across diverse sectors. Both the public and private sectors, with the developments of biometric technology, widely adopt and use an individual's biometric information for different reasons. For instance, smartphone users highly count on biometric technolgies for the purpose of security. Public and private orgazanitions control an access to confidential information-controlling facilities with biometric technology. Biometric infomration is known to be unique and immutable in the course of one's life. Given the uniquness and immutability, it turned out to be as reliable means for the purpose of authentication and verification. However, the use of biometric information comes with cost, posing a privacy issue. Once it is leaked, there is little chance to recover damages resulting from unauthorized uses. The governments across the country fully understand the threat to privacy rights with the use of biometric information and AI. The EU and the United States amended their data protection laws to regulate it. South Korea aligned with them. Yet, the authors point out that Korean data aprotection law still requires more improvements to minimize a concern over privacy rights arising from the wide use of biometric information. In particular, the authors stress that it is necessary to amend Section (2) of Article 23 of PIPA to reflect the concern by changing the basis for permitting the processing of sensitive information from 'the Statutes' to 'the Acts'.

Model Tests Investigating the Ground Movements Associated with Twin Side-by-Side Tunnel Construction in Clay (점성토 트윈 병렬 터널로 인한 지반침하 연구를 위한 모형실험)

  • Ahn, Sung-Kwon
    • Journal of the Korean Geotechnical Society
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    • v.25 no.10
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    • pp.77-85
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    • 2009
  • This paper describes the findings obtained from a research project aimed at investigating, via 1 g laboratory model tests, the ground movements caused by multiple side-by-side (sbs) tunnel construction in clay. The ground movements above a second tunnel showed different trends from those observed above a first tunnel. These trends include an increase in the overall volume loss, and a widening of the settlement troughs on the near limb of the trough accompanied by a shift of the maximum settlement towards existing tunnel. This would suggest that the use of simple predictive methods of adopting a Gaussian curve for analysing the ground settlements associated with twin (sbs) tunnel construction is not appropriate. Therefore the current paper adopts a method that modifies the Gaussian curve approach in order to improve the predictions. This paper comments on the parameter selection involved with adopting this new method to apply it to full-scale field situations, and also discusses its limitations.

A Study on the Actual Condition of Repair by Clothing Types and an Analysis of Repair Methods (의복 종류별 수선 실태조사와 수선방법에 관한 분석)

  • YeonHee Kim;MiKyeong Park;Jung-A Song
    • Fashion & Textile Research Journal
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    • v.25 no.3
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    • pp.324-332
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    • 2023
  • Consumers are addressing the issue of dimensional dissatisfaction by repairing clothes, experimenting with style changes, and looking for cost-effective solutions that result in better-fitting garments. This study investigated the repair status by type of clothing, analyzed the method and frequency of repair by type of clothing category and season, and analyzed consumer redesign activities. The findings revealed that upper garments, such as T-shirts, jumpers, jackets, dress shirts, and dresses, were frequently repaired. The common modifications to upper garments included(in order of frequency) shortening sleeve length, shortening overall length, reducing garment width, zipper repair, and adjusting sleeve width. Lower garments, such as pants, jeans, skirts, and training pants, followed in terms of repair frequency. The modifications to lower garments included(in order of frequency) shortening length, reducing width, adjusting waist width (both narrowing and widening), replacing elastic bands, zipper repair, and lengthening. Repairs were more frequently conducted in the order of autumn, winter, spring, and summer. Repair methods varied depending on the clothing type and alterations involved to the length and width of garments and the replacement or removal of old sections. Redesigning clothing as a recycling method was found to enhance the cost-effectiveness of the collection. The study further confirmed the sustainability aspect of redesigning and reusing clothing.