• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.04a
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• pp.254-259
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• 2006

• YAKHAK HOEJI
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• v.45 no.5
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• pp.522-528
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• 2001

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidase activity of tyrosinase. For the inhibitory effect against DOPA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and water, respective The methylene chloride fractions of Angelica tenuissima, Nardostachys chinensis, Bombyx mop and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

• Korean Journal of Medicinal Crop Science
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• v.19 no.6
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• pp.472-477
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• 2011

The Forsythiae Fructus is an oriental medicine containing various lignans. In this study, the Forsythiae Fructus were extracted by hot water (Sample 1), hot water after bio-conversion using Lactobacillus strain (Sample 2-LP2, 2-LA, 2-LC, 2-LL, 2-BL and 2-LM) and 70% ethanol (Sample 3). Total polyphenol and flavonoid contents were improved by bio-conversion process using Lactobacillus strain, compared to water extract. Especially, sample 2-LL and 2-LA which had shown the high total polyphenol and flavonoid content in antioxidant activity. Also, sample 2-LL and 2-LA showed higher melanin generation inhibitory activity as of 55%, 53% in maximum extract concentration of $100{\mu}g/m{\ell}$. In the anti-inflammation test of the Forsythiae Fructus extracts, nitric oxide (NO) synthesis was inhibited. Specially, both 70% Forsythiae Fructus ethanol extract and sample 2-BL which have shown the relatively higher 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD) like activities. In conclusion, the Forsythiae Fructus extracts with bio-conversion process has effect of skin whitening and anti-inflammation activity than other extracts. It could be used as a valuable materials for functional cosmetics.

• Korean Journal of Medicinal Crop Science
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• v.19 no.4
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• pp.264-270
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• 2011

In this study, we investigated the cosmetic application of Acer mono sap through an ultra-high pressure process. Exposing Acer mono sap to a ultra-high pressure process resulted in 90.1% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration. Acer mono sap also showed the hightest free radical scavenging activity after the ultra high pressure process. The melanogenesis inhibition rate in cloned M-3 cells was 59.0%. Tyrosinase was inhibited at a rate of 87.2% by adding 100% HPAMS. Anti-wrinkle activity was 78.1%. Acer mono sap showed enhanced storage following the ultra high pressure process. These results indicate that Acer mono sap may be a source for functional cosmetic agents capable of improving antioxidant, whitening, and antiwrinkling effects.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.3
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• pp.5-13
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• 2016

This study investigated the effects of different factors on the migration of a fluorescent whitening agent (FWA) from paper treated with FWAs to non-fluorescent papers. FWA migration experiments were carried out in vertical and friction contacts between the papers dyed with FWAs and non-fluorescent papers. During the experiments, we identified the effects of the addition and types of FWAs, contact time, temperature, and relative humidity (RH) on FWA migration. The fluorescence indices of the non-fluorescent papers were measured before and after the migration experiments, and the Student's t test, a statistical tool, was utilized to compare results from different migration experiments. In vertical contact experiments, FWA migration to non-fluorescent paper was observed at $30^{\circ}C$ and 70% RH; this was attributed to the high moisture content of the paper. FWA migration did not occur significantly at $23^{\circ}C$ and 50% RH. In the friction contact experiments, FWA migrations were identified at both temperature conditions and RH percentages. The addition and types of FWAs did not increase the fluorescence index of non-fluorescent papers. Therefore, it was concluded that the moisture content of paper and the friction contact affected FWA migration from the papers containing internal and surface FWAs.

• Bulletin of the Korean Chemical Society
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• v.31 no.5
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• pp.1319-1325
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• 2010

Tyrosinase ubiquitously existing from microorganisms to animals and plants is known to be the most critical and rate limiting enzyme during melanin biosynthesis. In order to develop new tyrosinase inhibitor we have synthesized 14 diphenyl ether compounds possessing hydroxyl, bromo, and nitro groups in the structure. Among the compounds prepared, 18 and 19 have shown much stronger inhibition of tyrosinase monophenolase function than arbutin used as a positive control. Both compounds 18 and 19 possess para-hydroxyphenyl moiety in their structure, which might reinforce the importance of p-hydroxyphenyl group in the tyrosinase inhibitory process. In the DPPH radical scavenging activity test, none of the compounds even 18 and 19 showed significant antioxidant activity. The results suggest that elaborate adjustment of diphenyl ether analogues with proper substituents have potential to be developed as new skin whitening agents working on the tyrosinase function.

• Textile Coloration and Finishing
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• v.29 no.4
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• pp.284-290
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• 2017

The glyoxal has been used as formaldehyde free DP(durable press) agents in wrinkle free treatment for cotton fabrics. However, the yellowing problem is a disadvantage of DP finishing process for cotton fabrics with glyoxal. In order to improve the whiteness, it was investigated that the effect of coreactant and treatment method with various whitening additives such as STB(sodium tetraborate, $Na_2B_4O_7$), SPB(sodium perborate, $NaBO_3$), SC(sodium chlorite, $NaClO_2$) and SPC(sodium percarbonate, $Na_2CO_3{\cdot}1.5$ $H_2O_2$). The increasing the concentration of whitening additives(STB, SPB, SC and SPC), the whiteness and the strength retention ratio of cotton fabrics were increased but the wrinkle recovery angles were decreased in one bath method. It was not suitable to improve whiteness because the whiteness value is about 60. In the case of SC used two bath method, the whiteness is near 70, which is similar to untreated fabrics, without decreasing of WRA and strength.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.15 no.2
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• pp.104-117
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• 2002

Objective : The aim of this study was to investigate the skin-whitening effect of Kakamseosiokyong-san Method : We investigated that the extracts of Kakamseosiokyong-san inhibit activity of tyrosinase, the enzyme which converts 3-(3,4-dihydroxyphenyl)alanine to dopachrom in the biosynthetic process of melanin. the UV absorbance of the extracts in the UV - A region and UV - B region was measured by UV scanning. the effect of extracts on cell viability and melanin production in cultured B16 mouse melanoma cells was measured, and cytoprotective effects of extracts on PC12 cells injured by hydrogen peroxide was measured by MTT assay Results: The extracts of Kakamseosiokyong-san inhibited activity of tyrosinase. The extracts not only showed inhibitory effects on melanin production in cultured B16 mouse melanoma cells, but also exhibited cytoprotective effects on PC12 cells injured by hydrogen peroxide, but did not showed an absorbance in the UV - A region and UV - B region. Conclusion: These results suggest that Kakamseosiokyong-san inhibit melanin biosynthesis which is involved in hyperpigmentation and could be used as a whitening agent for the skin.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.98-98
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• 2019

This study was expand the use of Citrus madurensis (Citrofortunella microcarpa), which is a good source of skin whitening and antioxidants activation cosmetics. The goal was to provide cosmeceutical activity data about the extraction yield and total phenol of this calamondin orange by optimizing the focused high ultrasound(INEFU) and ultrasound extraction(UE) conditions. Under optimal extraction conditions, which consisted of 1800W for 45min and 500W for 45 min, 21.55 and 13.27 % (w/w) of the highest extractions yield and polyphenol contents were obtained. For the skin whitening activity, tyrosinase inhibitory activity was observed at 69.24 % in the INEFU extracts, which was ca. 18 % higher than that of the UE extracts (58.82 %). To reduce melanin production in Clone M-3 cells, 86.9 % melanin production was observed following the addition of control, WE(water extraction) relative to the control without a sample, and 81.2 and 78.9 % were found in the UE and INEFU conditions. The highest total phenol secretion was conclusively obtained under the optimal conditions and resulted in a significant improvement of the cosmetic activities of Citrus madurensis(Citrofortunella microcarpa) extracts.

• 한국약용작물학회:학술대회논문집
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• 2007.05a
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• pp.155-156
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• 2007