• Proceedings of the Korean Society of Applied Pharmacology
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• 2005.04a
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• pp.131-148
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• 2005

This study is peformed to investigate the effect of water extract from Vaccinium uliginosum L., on melanin production in B 16 melanoma cells, procollagen production and matrix metalloproteinase-1(MMP-1) inhibition in human fibroblast cells. One hundred grams of the Vaccinium uliginosum L. was extracted with 2000 mL of water($90^{\circ}C$, 16h, 2times). The water extracts were lyophilized and stored at $4^{\circ}C$ until used. Dry weight yields of extracts of Vaccinium uliginosum L. were $3\%$(w/w). Extracts from Vaccinium uliginosum L. showed scavenger activities on DPPH radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide and singlet oxygen radical. And these substances inhibited release of cyiokines from human keratinocyte after UV B exposure. Therefore we confirmed that extracts from Vaccinium uliginosum L. had antioxidative effect. These substances inhibited purified tyrosinase activity and melanogenesis in B 16 melanoma cells treated/untreated IL-$1{\alpha}$. Moreover this extract stimulated procollagen production and inhibited MMP-1 production in human fibroblast cells treated/untreated IL-$1{\beta}$. Therefore we confirmed that extracts from Vaccinium uliginosum L. had whitening effect. And these substances decreased degree of wrinkle in hairless mouse skin that induced by UV B irradiation. Therefore we confirmed that extracts from Vaccinium uliginosum L. had anti-wrinkle effect. From the above results, it is possible that Vaccinium uliginosum L. may be developed to be an anti-melanogenesis agent and anti-wrinkle agent.

• Korean Journal of Pharmacognosy
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• v.36 no.4 s.143
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• pp.273-277
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• 2005

In mammalian melanocytes, melanin synthesis is controlled by tyrosinase, the key enzyme in the pigment synthesis. In this study, to develop a new whitening agent, we have investigated the antioxidant and the inhibitory effect of Artemisia anomala extract on tyrosinase activity and melanigenesis in the B16/F1 melanoma cells. The inhibition ratio of tyrosinase activity of butanol fraction from A. anomala was higher than that of arbutin ($97.5{\pm}0.5%$ at the concentration of 2 mg/ml). The butanol fraction was shown scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals in a dose dependent manner. The highest inhibitory activity of melanogenesis was also butanol fraction ($25.0{\pm}3%$ at the concentration of $200\;{\mu}g/ml$). From these results, we suggest that the A. anomala extract might be used to be a potential agent for skin whitening.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.43 no.2
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• pp.9-15
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• 2011

Fluorescent whitening agent (FWA) is a widely used chemical in paper industry, but a systematic and scientific method on FWA analysis has not been established. We performed the basic researches on the fluorescence analysis of FWA. The fluorescence of FWA was investigated using a spectrofluorometer and a spectrophotometer. When FWA solution was analyzed using the spectrofluorometer, we found that the peak wavelength of the fluorescence emission was about 440 nm and that of the fluorescence excitation was about 370 nm irrespective of FWA types. Papers dyed with an internal FWA were prepared in a laboratory and the reflectance and the fluorescence index were measured using the spectrophotometer. It was confirmed that the optimum peak wavelength of the reflectance was 440 nm and the fluorescence index calculated from the CIE whiteness with and without UV light under a light source D65 was the best indicator to measure the fluorescence of FWAs exiting in papers.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.24 no.3
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• pp.45-50
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• 1998

13 develop an active agent for skin whitening, we examined the inhibitory effects of 285 plant extracts on tyrosinase activity and found some plant extracts have an ability to inhibit tyrosinase activity. Of these plant extracts, ramulus mori extracts showed the highest inhibiting activity on enzyme activity. From these results, we selected ramulus mori as a new active agent for skin whitening. $IC_{50}$/ of this extracts on tyrosinase activity is 12.48$\mu\textrm{g}$/m1. Also, ramulus mori extracts showed inhibitory activity on melanin synthesis in B-16 melanoma cells. We isolated active compound from ramulus mori extracts using various column chromatography and recrystalization and identified its chemical structure using Mass spectroscopy, IR and NMR analysis. So, it is identified as Mulberrin, a maior compound of ramutus mori and turned out that it has potent ability on tyrosinase inhibition. $IC_{50}$/ of this compound for tyrosinase activity is 0.5$\mu\textrm{g}$/ml. Also, this compound showed potent inhibitory activity on melanin synthesis in B-16 melanoma cells. This extracts was turned out to be safe in many safety tests.

• Korean Journal of Plant Resources
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• v.34 no.6
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• pp.510-516
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• 2021

Whitening agents derived from natural sources which do not have side effects are sought after. Schizophragma hydrangeoides is an edible plant that grows wild on Jeju Island. We aimed to determine whether S. hydrangeoides extracts show anti-melanogenic activity. Here, we found that 70% ethanol extracts of S. hydrangeoides leaf suppressed α-melanocyte-stimulating hormone-induced melanogenesis in B16F10 mouse melanoma cells. This activity of anti-melanogenesis in B16F10 cells were investigated by determining melanin content and tyrosinase activity, and by performing western blotting. The 70% ethanol extract downregulated tyrosinase and tyrosinase-related protein 1. In addition, the n-hexane fraction of S. hydrangeoides leaf (HFSH) exhibited significant anti-melanogenic activity among the various solvent fractions tested without reducing the viability of B16F10 cells. Taken together, these results indicate that extracts from S. hydrangeoides leaf can influence cellular processes via modulation of tyrosinase activity. Hence, S. hydrangeoides can be used as a whitening agent in the cosmetic industry and as a therapeutic agent for treating hyperpigmentation disorders in the clinic.

• Textile Coloration and Finishing
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• v.29 no.1
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• pp.45-54
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• 2017

Ultraviolet radiation have much influenced with a deep wrinkles, roughness, laxity of skin damage and pigmentation through oxidative stress and oxidative photo-damage. This study investigates the functional properties of hydrogel facial mask sheets made from agar, Styela clava tunics and Broussonetia papyrifera tunics. The skin of S. clava is covered with a hard cellulose containing glycoprotein, glycosaminoglycan and chondroitin sulfate. B. papyrifera is better known as Paper mulberry. It contains kazinol which serves as a tyrosinase inhibitor and skin whitening agent. The tensile strength of facial mask sheet was measured by universal testing machine, and the water absorption and moisture permeability of facial mask sheet were measured by dryer. Additionally, the DPPH assay and MTT assay were conducted for anti-oxidative activity and cytotoxicity of facial mask sheet. The whitening effect of the facial mask sheet was measured by tyrosinase inhibitor assay. These tests showed that the three ingredients are suitable cosmetic materials. The results reveal that they produce a high quality hydrogel facial mask sheet when the membrane contains 1%(W/V) of agar, 0.1%(W/V) of B. papyrifera tunics and 0.05%(W/V) of S. clava tunics.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.3
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• pp.5-13
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• 2016

This study investigated the effects of different factors on the migration of a fluorescent whitening agent (FWA) from paper treated with FWAs to non-fluorescent papers. FWA migration experiments were carried out in vertical and friction contacts between the papers dyed with FWAs and non-fluorescent papers. During the experiments, we identified the effects of the addition and types of FWAs, contact time, temperature, and relative humidity (RH) on FWA migration. The fluorescence indices of the non-fluorescent papers were measured before and after the migration experiments, and the Student's t test, a statistical tool, was utilized to compare results from different migration experiments. In vertical contact experiments, FWA migration to non-fluorescent paper was observed at $30^{\circ}C$ and 70% RH; this was attributed to the high moisture content of the paper. FWA migration did not occur significantly at $23^{\circ}C$ and 50% RH. In the friction contact experiments, FWA migrations were identified at both temperature conditions and RH percentages. The addition and types of FWAs did not increase the fluorescence index of non-fluorescent papers. Therefore, it was concluded that the moisture content of paper and the friction contact affected FWA migration from the papers containing internal and surface FWAs.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.2
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• pp.5-12
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• 2016

White waste papers are very important resources in the paper industry, but their use is limited because of the residual of fluorescent whitening agent (FWA). So the removal of FWAs from waste paper is an important task in the recycling process to improve the use of recycled resources. In this study, we focused on the FWAs used for surface treatments and carried out physical and chemical treatments to remove them from white waste papers. The white waste papers were disintegrated with a surfactant in different pH and temperature conditions, and then handsheets were made for the measurement of the fluorescence index, which is proportional to the amount of FWAs on papers. The effect of the flotation process on the removal of FWAs after disintegration was also investigated. The fluorescence index decreased as the disintegration time increased, but over a relatively long time, the fluorescence index increased again, which indicated the readsorption of the FWAs detached from the cellulosic fibers of the white waste papers. The lowest fluorescence index was shown when the waste papers were disintegrated with a 0.3% surfactant addition at pH 10 and at $45^{\circ}C$. However, the flotation treatment was not effective, because the flotation induced contact between the detached FWAs and the cellulosic fibers, and re-adsorption occurred.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.47 no.6
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• pp.106-112
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• 2015

Many researchers have proposed analytical methods to measure the adsorption of di-sulpho fluorescent whitening agents (D-FWAs), but practical methods for D-FWA utilization in an actual paper mill have not been established. In particular, the D-FWA adsorption behavior must be monitored in paper mills to ensure the effective use of D-FWAs. This study used the zeta-potential of pulps as an indicator of the adsorption behavior of a D-FWA. We identified the relationship between the actual adsorption of the D-FWA and the zeta-potential of the pulps as a function of D-FWA addition. zeta-potential measurements were then used to analyze the D-FWA adsorption behavior under different conditions of pulp type, conductivity, and pH. The actual adsorption of a D-FWA was proportional to the ${\Delta}zeta-potential$ of the pulps (i.e., the difference between the zeta-potential of a pulp containing no D-FWA and one containing the D-FWA). The ${\Delta}zeta-potential$ of the pulps was therefore adopted for adsorption analysis. A higher adsorption of the D-FWA was observed onto Hw-BKP than onto Sw-BKP because of the shorter fiber length and higher fines content of Hw-BKP. A high conductivity and an acidic pH decreased the D-FWA adsorption because of direct effects of high ion concentrations and low pH on the D-FWA solubility. Therefore, a D-FWA must be added to Hw-BKP under low conductivity conditions and at neutral or alkaline pH to optimize the D-FWA adsorption.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.29 no.1
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• pp.151-167
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• 2003

Melanin biosynthesis is a human defense mechanism to protect skin from UV irradiation and also determines colors of hair and skin. However, as a interest on skin-whitening increases, researches to prevent pigmentation and hypersynthesis of melanin in skin are being actively in progress. Active components used as a whitening agent in cosmeceuticals are kojic acid, arbutin, vitamin C and hydroquinone. However, until now, because comparison researches among them in the aspect of both melanin formation and cellular toxicity have not been performed, we can't exactly estimate merits and defects of them as a whitening agent. To this end, we performed experiments to compare their effects on cell viability and melanin formation. As a first step, in vitro tyrosinase inhibition assay was done. While kojic acid and hydroquinone showed strong inhibition activities(their IC$\_$50/s are all < 100uM), arbutin and vitamin C showed weak activities. IC$\_$50/s of arbutin and vitamin C are 100uM and 400∼500uM, respectively. In B16BL6 melanoma cells, like in vitro tyrosinase inhibition assay, arbutin and kojic acid showed more strong inhibition effect on melanin synthesis than vitamin C. And unlike arbutin, vitamin C and kojic acid induced cell death at high concentration. Although arbutin showed no cytotoxicity, it has side effect to induce morphological change at high concentration.. In this paper, we suggest both kojic acid and arbutin have stronger ability to inhibit melanogenesis than vitamin C. And they also have side effect, that is, kojic acid induces cell death like vitamin C and arbutin changes cell morphology respectively.