• Title/Summary/Keyword: white line.

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Genomic Regions associated with Necrotic Enteritis Resistance in Fayoumi and White Leghorn Chickens

  • Kim, Eui-Soo;Lillehoj, Hyun S.;Sohn, Sea Hwan;Hong, Yeong Ho
    • Korean Journal of Poultry Science
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    • v.42 no.1
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    • pp.27-32
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    • 2015
  • In this study, we used two breeds of chicken to identify genomic regions corresponding to necrotic enteritis (NE) resistance. We scanned the genomes of a resistant and susceptible line of Fayoumi and White Leghorn chickens (20 birds/line) using a chicken 60 K Illumina SNP panel. A total of 235 loci with divergently fixed alleles were identified across the genome in both breeds; particularly, several clusters of multiple loci with fixed alleles were found in five narrow regions. Moreover, consensus 15-SNP haplotypes that were shared by the resistant lines of both breeds were identified on chromosomes 3, 7 and 9. Genes responsible for NE resistance were identified in chicken lines selected for resistance and susceptibility. Annotation of the regions spanning clustered divergently fixed regions revealed a set of interesting candidate genes such as phosphoinositide-3-kinase, regulatory subunit 5, p101 (PIK3R5) and inositol 1,4,5-trisphosphate receptor 1 (ITPR1), which participate in immune response. Consensus haplotypes were found in regions containing possibly relevant genes, such as myostatin and myosin, which play important roles in muscle development. Thus, genome scans of divergent selection in multiple chicken lines and breeds can be used to identify genomic regions associated with NE resistance.

Cloning of a DNA Fragment Specific to Pseudomonas tolaasii Causing Bacterial Brown Blotch Disease of Oyster Mushroom (Pleurotus ostreatus) (느타리버섯 세균성갈색무늬병 병원균 Pseudomonas tolaasii의 특이적 DNA 클로닝)

  • 이혁인;차재순
    • Korean Journal Plant Pathology
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    • v.14 no.2
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    • pp.177-183
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    • 1998
  • A DNA fragment which is involved in tolassin production was cloned to obtain a molecular marker of Pseudomonas tolaasii, a casual agent of bacterial brown blotch disease of oyster mushroom (Pleurotus ostreatus). Tolaasin is a lipodepsipeptide toxin and known as a primary disease determinant of the P. tolaasii. It is responsible for formation of white line in agar when P. tolaasii were cultured against white line reacting organisms (WLROs). White line negative mutants (WL-) were generated by conjugation between rifampicin resistant strain of P. tolaasii and E. coli carrying suicidal plasmid pSUP2021 : : Tn5. The ability of tolaasin production of the WL- mutants was examined by hemolysis test, pathogenicity test, and high pressure liquid chromatography (HPLC) analysis of culture filtrate. All of the WL- mutants were lost the ability of tolaasin production (Tol-). Genomic library of the Tol- mutant was constructed in pLAFR3 and the cosmid clone containing Tn5 was selected. DNA fragment fro franking region of Tn5 was cloned from the plasmid and used as a probe in Southern blot. DNA-DNA hybridization with the probe to total DNA from group of bacteria ecologically similar to P. tolaasii including WLORs, fluorescent Pseudomonads isolated from oyster mushroom, P. agarici, P. gingeri, and some of other species of Psedomonas showed that some of the tested bacteria do not have any hybridized band and others have bands sowing RFLP. The cloned DNA fragment or its nucleotide sequence will be useful in detection and identification of the P. tolaasii.

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X-ray Absorption Near-edge Studies of Au1-xPtx alloys

  • Y.D. Chung;Lim, K.Y.;Lee, Y.S.;C.N.Whang;Park, B.S.;Y.Jeon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.164-164
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    • 2000
  • Since Au-Pt alloys have various atomic structures depending upon composition and annealing temperature, it is very interesting to investigate the electronic structures of alloys. We studied the changes of the electronic structure I the Au-Pt alloys by x-ray absorption near edge spectroscopy (XANES). Two kinds of Au-Pt alloy samples were prepared by arc melting methods and ion-beam-mixing technique. The Pt L2, 3-edge and Au L2, 3-edge X-ray absorption spectra (XPS) were measured with the electron yield mode detector at the 3C1 beam line of the Pohang Light Source (PLS). It was found that there was a substantial decrease in the area of the Pt L2, 3 white lines compared with that of pure Pt. The observed decrease in white line area was attributed to an increase in the number of pure Pt. The observed decrease in white line area was attributed to an increase in the number of 5d-electrons at the Pt site upon alloy formation. However, the Au L2, 3 edge spectra for Au-Pt alloys are all similar to that of pure Au. This implies that the 5d hole count of Au is not changed by alloy formation with Pt.

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LINE EMISSION FROM THE MAGNETOSPHERE OF MAGNETIC CATACLYSMIC VARIABLES (MCV 자기구에서의 선방출)

  • KIM YONGGI
    • Publications of The Korean Astronomical Society
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    • v.15 no.spc1
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    • pp.113-118
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    • 2000
  • A magnetic cataclysmic variable has a rotating magnetic white dwarf which accretes matter from its late type companion. Kim & Beuermann (1995) presented a phenomenological model of the accretion from its surrounding structure e.g., a disk into the magnetosphere of the white dwarf, and presented results for the spin modulated X-ray spectrum and light curves. Using this model, we calculate the optical continuum and line emission which result from reprocessing of X-rays in the accretion stream within the magnetosphere. Penning (1985) suggested the observed spin-modulated radial-velocity variations might result from reprocession of X-rays in the disk. We, however, find the radiation can be originated from the magnetosphere accretion stream. We use the same geometrical model to calculate the optical and the X-ray behaviour. The results from the two wavelength bands are internally consistent. We conclude that this approach will increase the diagnostic accuracies of the results.

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Effects of Rearing Systems on Performance, Egg Characteristics and Immune Response in Two Layer Hen Genotype

  • Kucukyilmaz, Kamil;Bozkurt, Mehmet;Herken, Emine Nur;Cinar, Mustafa;Catli, Abdullah Ugur;Bintas, Erol;Coven, Fethiye
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.4
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    • pp.559-568
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    • 2012
  • White (Lohmann LSL) and Brown (ATAK-S) laying hens, were reared under organic and conventional cage rearing systems, and the effects of the rearing system on performance parameters, egg production, egg characteristics, and immune response were investigated. For this purpose, a total of 832 laying hens of two commercial hybrids, i.e., 416 white (Lohmann LSL) and 416 Brown (ATAK-S) layers, were used. The experiment lasted between 23 and 70 wk of age. In this study, the white layers yielded more eggs as compared to the brown layers in both organic and conventional production systems. Egg weight exhibited a similar pattern to that of laying performance. However, the total hen-housed egg number for the white birds in the organic system was fewer than that of white birds in the conventional cage facility; conversely, a contradictory tendency was observed for the brown birds. Livability of the white layers in the organic system was remarkably lower (14%) than that of the brown line, whereas the white line survived better (3.42%) than their brown counterparts in conventional cages. The feed conversion ratio of the white hens was markedly inferior in the organic system as compared to that of the white hens in the conventional system, whereas relatively lower deterioration was reported in brown layers when reared in an organic system. The organic production system increased egg albumen height and the Haugh unit in eggs of the brown layers. The yolk color score of organic eggs was lower than that of conventional eggs for both brown and white hens. The egg yolk ratio of eggs from white layers was found to be higher in organic eggs as compared to those obtained in the conventional system. All organic eggs had heavier shells than those produced in the conventional system. Eggs from brown layers had more protein content than eggs from white layers. Neither housing systems nor genotype influenced egg yolk cholesterol concentration. When compared to conventional eggs, n-3 fatty acid content was lower in organic eggs, and the n-6:n-3 ratio was higher in organic eggs. In conclusion, two hen genotypes showed different responses in terms of performance and egg quality to two different rearing systems. A commercial white strain produced more eggs with higher egg quality as compared to a native brown strain. The brown strain was found to have adapted well to organic production conditions when survival and total egg number was taken into consideration.

Approach to Specify a Component using Component Structure in Product Lines (제품 라인에서 컴포넌트 구조를 활용한 컴포넌트 스펙 방법)

  • Cho Hye-Kyung
    • Journal of KIISE:Software and Applications
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    • v.33 no.3
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    • pp.289-300
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    • 2006
  • Product line is nowadays well known as a representative method for reuse. In the product line, important assets are components. Although enough concerns were given of the product line, it was not accomplished to structure and specify a product-line component with variability. This paper presents an approach to specify components in the product line. The approach describes the static and dynamic structure of a product-line component and explains the behavior and concurrency of the component. The component information is separately described in the black-box and white-box using the Feature-Oriented Reuse Method(FORM). This research also formalizes the data on a component specification in the form of BNF. The specification is described through careful consideration for many different characteristics of the product-line component, so this paper helps to easily develop the components in the product line and to well comprehend how to apply a method for the product line.

THEORETICAL LINE PROFILES OF THE MAGNETIC COMPACT STARS

  • KIM YONGGI
    • Journal of The Korean Astronomical Society
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    • v.29 no.spc1
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    • pp.235-236
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    • 1996
  • Using a phenomenological model for the accretion onto the magnetic white dwarf, we calclliated some optical line profiles from the magnetosphere of such systems. Line profiles of these systems seem to be produced in the magnetosphere of the compact star due to the reemission of X-ray produced near the stellar surface. Some results of our new calculation and the analysis of these results will be presented. Our results show that the model used here can reproduce the observed optical line profiles and open the possiblity to determine the parameters of individual systems.

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A Comparative Biological Study of the Rhizome and Main Root from Red and White Ginsengs (홍삼 및 백삼의 뇌두, 동체에 대한 생물활성 비교)

  • Park, Jong-Dae;Wee, Jae-Joon;Kim, Young-Sook;Kim, Si-Kwan;Park, Ki-Hyun
    • Journal of Ginseng Research
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    • v.20 no.3
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    • pp.256-261
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    • 1996
  • Comparative biological activities of 70fr methanol extracts from the main roots and rhizomes of both red and white ginsengs were investigated using several in vitro experimental models. The main root of red ginseng and the rhizome of white ginseng strongly inhibited lipld peroxidation of hepatic microsomes induced by the non-enzymatic $Fe^{+}$ / Ascorbate system. The main root and rhizome of red ginseng markedly inhibited the release of G07, GPT and LDH by $CCl_4$-induced cytotoxicity in primary cultured rat hepatocytes as compared with those of white ginseng. And also, the main root of red ginseng showed a slight differentiating activity on HL-60 cancer cell line. The results suggest that the rhizome of ginseng have potential as a source of medicinal crude drug with possible pharmacolobica1 applications .

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THE FE Kα EMISSION LINE OF INTERMEDIATE POLAR V1223 SAGITTARII

  • Nwaffiah, J.U.;Eze, R.N.C.
    • Journal of The Korean Astronomical Society
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    • v.47 no.4
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    • pp.147-152
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    • 2014
  • We present measurements of the Fe $K{\alpha}$ emission line of the intermediate polar V1223 Sagittarii observed with the Suzaku satellite. The spectrum is modeled with an absorbed thermal bremsstrahlung spectrum and three Gaussians for the three components of the Fe $K{\alpha}$ lines. We resolve the neutral or low-ionized (6.41keV), He-like (6.70keV), and H-like (7.00keV) iron lines. We also obtain a thermal continuum temperature of 25 keV, which supports a thermal origin of the hard X-rays observed from the shock heated layers of gas between the white dwarf and the shock front. Hence, we believe that the He-like and H-like lines are from the collisional plasma. On the origin of the Fe $K{\alpha}$ fluorescence line, we find that it could be partly from reflections of hard X-rays from the white dwarf surface and the $N_H$ absorption columns. We also discuss the Fe $K{\alpha}$ emission line as veritable tool for the probe of some astrophysical sites.

Effect of Work Environment and Low Back Pain on the Structural and Muscle Strength Changes in Lumbar Spine (작업환경과 요통이 요추의 구조 및 근력의 변화에 미치는 영향)

  • Kim, Na-Yeon;Kang, Jae-Hui;Lee, Hyun
    • Journal of Acupuncture Research
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    • v.27 no.3
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    • pp.93-104
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    • 2010
  • Objectives : The purpose of this study is to observe the effects of work environment and low back pain on the structural and muscle strength changes in lumbar spine to helpful for preventation and cure of low back pain. Methods : Through measuring of lumbosacral angle, lumbar lordotic angle, lumbar gravity line ratio analyzed structure of lumbar spine and using Trunk Extension Flexion Program of CYBEX NORM System(cybex770+TMC, USA) analyzed Flex. PT, Ext. PT, E/F ratio of lumbar spine of company employees given a medical examination. Results : According to work environment, lumbar gravity line ratio is higher in white collar group than in blue collar group, Ext. PT is significantly lower in white collar group than in blue collar group. According to low back pain or not, lumbar gravity line ratio, Ext. PT is lower in low back pain group than in non-low back pain group. Conclusions : Work environment and low back pain effects on the structural and muscle strength changes in lumbar spine.