• International Journal of Industrial Entomology and Biomaterials
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• 제41권2호
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• pp.45-50
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• 2020

Morus alba has white and/or purple fruits with a very sweet taste and low acidity. Most Korean mulberry trees have purple fruits. However, Baekokwang is a unique mulberry genetic resource in Korea with white fruits. In this study, flavonoids contents of Baekokwang mulberry leaf and fruit were analyzed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS) technique. UPLC-DAD-QTOF/MS chromatogram showed that 15 flavonoids and 9 flavonoids were isolated and identified from the mulberry leaf and fruit. Total flavonoids contents of Baekokwang leaves and fruits were 812.7 mg and 35.0 mg, respectively. Baekokwang leaves had 4 major flavonoids including quercetin 3-O-(6"-O-malonyl) glucoside, 235.3 ppm, kaempferol 3-O-(6"-O-malonyl) glucoside, 132.3 ppm, kaempferol 3-O-rutinoside (nicotiflorin), 108.1 ppm, and quercetin 3-O-rutinoside (rutin), 103.8 ppm. Baekokwang fruits had 3 major flavonoids including quercetin 3-O-(6"-O-malonyl) glucoside, 13.0 ppm, quercetin 3-O-rutinoside (rutin), 7.8 ppm, and kaempferol 3-O-rutinoside (nicotiflorin), 5.7 ppm. From the above results, mulberry leaves have rich flavonoids compared to its fruits.

• 한국작물학회지
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• 제36권5호
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• pp.459-480
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• 1991

인삼의 생리장해를 산지중심으로 증상별로 종합검토하였다. 뿌리의 장해는 적피, 은피, 동해, 동해, 근부, 안삼, 출아불재, 달래삼, 소수가 있고 지상부에는 황엽, 조기낙엽, 소엽, 지엽, 백반엽, 동해, 풍해와 경열이 있다. 적피와 황엽이 인삼생산에 가장 크게 영향한다. 농약해, 붕소과 잉해 및 산업공해에 의한 피해 경우도 보고되었다. 원료수삼 가공한 후의 품질요인에 관계되는 생리장해들도 검토하였다.

• Journal of Plant Biotechnology
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• 제36권2호
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• pp.193-196
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• 2009

Pinellia koreana K-H Tae & J-H Kim is a recently discovered Korea endemic medicinal plant species whose natural habitat is rapidly destroyed by industrial development. Described in this paper are culture conditions for high frequency plant regeneration via bulblet formation from leaf explant cultures of P. koreana. Leaf explants formed white nodular structures and off-white calluses at a frequency of 91.2% when cultured on MS medium supplemented with 2 mg/L BA and 0.5 mg/L NAA. However, the frequency of white nodular structures and off-white calluses formation was slightly decreased with an increasing concentration of NAA up to 4 mg/L, where the frequency reached 31.7%. Most petiole explants did not form white nodular structures and off-white calluses except the combination treatment of 2 mg/L BA and 2 mg/L NAA. Upon transfer onto MS basal medium, over 90% of nodular structures gave rise to numerous bulblets and developed into plantlets. Plantlets regenerated from bulblets were transplanted to potting soil and grown to maturity at a survival rate of over 95% in a growth chamber. Therefore, the in vitro plant regeneration system of P. koreana obtained in this study will be useful for mass propagation and long-term preservation of genetic resources of P. koreana.

• Journal of Plant Biotechnology
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• 제30권2호
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• pp.207-214
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• 2003

This study was carried out to establish a novel bioassay system for screening of compounds affecting aromatic amino acid or anthocyanin biosynthesis through investigating a degree of sucrose-induced anthocyanin formation such as size of plant material, buffer conditions, light intensity and irradiated duration, incubation temp., etc were determined and standard procedure (suitable experimental condition) was set up as follows. The second leaf blade of white corn seeding induced by fluridone treatment were segmented into a size of 5${\times}$5 min. The segments were floated on the solution of 1% sucrose in 1.0mM MES buffer (pH6.0∼6.5) and incubated at 26$^{\circ}C$ for 2days under the continuous light condition(70∼100$\mu$mol m$^{-2}$ s$^{-1}$ ). Anthocyanin in the purpled tissues was extracted with methanol containing 1% HCl and the optical density of the clear supematants was determined at 528mm. Influences of some chemicals were tested using this system. Glyphosate, 5-enolpyruvylshikimate 3-phosphate synthase inhibitor, showed most sensitive response with I$_{50}$ value at 3.3$\mu$M. Dicyclohexylcarbodiimide(DCCD) and parachloromercuribenzenesulfonic acid(PCMBS) had a relatively strong ingibition with I50 value at 7.1$\mu$M and 10.2$\mu$M, respectively. These results show that sucrose-induced anthocyanin formation in white com leaf segment provide a very simple and rapid system for searching new compounds affecting aromatic amino acid or anteocyanin biosynthesis by screening at less than 10$\mu$M.

• Plant Biotechnology Reports
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• 제3권3호
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• pp.191-197
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• 2009

Four-leaf white clover is not found easily in nature due to its low appearance rate (1 in 10,000). Because people believe that it brings good luck and like to either keep it or present it to a loved one, it has commercial and ornamental value. To breed four-leaf clover, we exposed its flowers to ${\gamma}-rays$ at the pollination stage. The $M_1$ seeds produced following doses at 25-100 Gy showed an approximately 74% germination rate, with seedling survival at 46%. In the $M_1$ generation of plants irradiated within that dose range, we found an increased frequency of four leaflets. One of them, Jeju Lucky-1 (JL-1), had a frequency of about 60%. To see whether that mutation was somaclonal or genetic, we observed its $M_2$ generation and found that such a phenotype reappeared. Although our results demonstrated that the irradiation of fully mature flowers led to a higher frequency of 4-leaflets, we could not clearly explain the genetic mechanism involved. We suggest that JL-1 is valuable as a new variety, without further genetic fixation, because white clover can be propagated vegetatively by stolons.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.52-52
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• 2019

Buckwheat is well-known crop for containing a high contents of flavonoids that are effective in vascular disease. The current study was performed to estimate the influence of morphological characterization of Fagopyrum esculentum (ES) germplasm for seed's two major flavonoids contents: rutin and quercetin. We found that the red stem color, pale green leaf color, arrowhead leaf shape, white flower color, pale brown seed coat color, and egg-shaped seed were significantly associated with 77%, 56.7%, 83.7%, 98.7%, 70.8% and 74.5% germplasm, respectively. Overall, the rutin contents of ES germplasm ranged from 0.30 to 47.86 mg/100g dry weight (DW) and the quercetin contents ranged from 0 to 1.22 mg/100g DW. The rutin contents of germplasm possessing red stem color, pale green leaf color, arrowhead leaves, white flower color, pale brown seed coat color and egg-shaped seed ranged from 7.22 to 47.86 mg/100g DW. However, the quercetin contents of germplasm with red stem color and pale brown seed coat color ranged from 0 to 1.15 mg/100g DW, with pale green leaves ranged from 0 to 0.96 mg/100g, with arrowhead leaves and white flower ranged from 0 to 1.22 mg/100g and with egg-shaped seed ranged from 0.32 to 1.22 mg/100g DW. In PCA analysis, the first three principal components (PCs) showed Eigen value more than 1 and accounted for 51.70% of variation. For both higher contents of rutin and quercetin, the morphological evaluation in ES shows a tendency of red stem color, arrowhead leaves, pale green leaf color, white flower color, pale brown seed coat color and egg-shaped seed. From this information, we can assume the rutin and quercetin contents by the morphological characteristics of the germplasm. And It could be useful in improving the rutin and quercetin contents and selecting proper resources for cultivation in existing buckwheat cultivars.

• 생물환경조절학회지
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• 제31권1호
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• pp.60-66
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• 2022

본 연구는 파프리카 육묘 시 다양한 LED 광질(red:blue = 10:0, 8:2, 2:8, white)에 따른 생육변화에 대하여 구명하여 수경재배용 묘 생산을 위한 전용육묘장의 기반기술로 활용하고자 수행하였다. 초장과 줄기직경의 생육은 red 비율이 높을수록 유의하게 길거나 굵었고, 엽면적은 LED red:blue = 8:2에서 가장 넓었다. 건물 중량도 엽면적과 동일한 경향이었다. LED white에서는 모든 묘의 소질에서 다른 처리들보다 뚜렷하게 저조하였다. 상대생장률은 red 비율이 높을수록 높은 경향을 나타내었고 순동화율은 blue 비율이 높을수록 높은 경향을 나타내었다. 따라서 본 시험에서 LED를 활용한 파프리카 육묘 시 초장, 엽면적, 단위엽면적당 건물생산능력 등을 고려하였을 때 혼합광을 이용하는 것이 적합하고 특히, LED red:blue = 8:2가 가장 적합한 것으로 생각된다. 그리고 red와 blue 파장의 적절한 혼합 비율을 통해 파프리카 묘의 초장 및 절간장을 제어할 수 있을 것으로 생각된다.

• 한국작물학회지
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• 제37권3호
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• pp.264-273
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• 1992

White clover는 여러 가지 장점에도 불구하고 조기정착과 조성이 어렵고 단속방목에 대한 적응력 부족으로 초지내에서의 존속년한이 짧아 이용성이 제한되어 왔다. 따라서 본 시험은 white clover를 기존초지에 도입하는데 필요한 정보를 제공하기 위하여 최초예취시기(trifoliolate 1, 4, 8)의 조만과 예취간격 (7일, 28일)이 28일의 재생기간 동안 엽의 크기가 다른 white clover 품종들(Regal, La S.1, Huia 및 S184)의 수량과 재생에 미치는 영향을 조사하기 위하여 온실에서 pot 시험으로 실시한 바 그 결과를 요약하면 다음과 같다. 1. 28일 동안의 수확 건물중은 최초예취시기를 빨리하거나 예취간격을 줄임으로써 현저히 감소한 반면, 예취간격에 관계없이 최초예취시기를 늦춤으로써 지품종에 비하여 대업형 Regal이 높았다. 2. 지상부 및 뿌리의 건물중은 재생 초기 (7일)에는 최초예취시기를 늦추고 예취간격을 늘임으로써 증가하나 충분한 기간 동안 재생(28일)이 허용될 때 완화되는 경향을 보였고, 전재생기에 걸쳐 Regal이 지품종에 비하여 근중 및 전식물체중은 높았다. 3. 재생기간 동안 전식물체중의 증가는 주로 지상부 건물중의 증가에 기인하였고, 예취간격 28일에서는 예취시의 건물중으로 회복하는 데는 약 2주가 걸렸다. 4. 식물개체당 엽면적과 예취간격 7일에서 현저히 감소되었고, 재생 7일에서는 최초예취시기가 빠를수록 감소하나 재생 28일에서는 차이가 없었다. 5. 포복경장과 분지경수는 소엽형 S184에서 가장 길고 많았으며 재생기간 동안 급겹히 증가한 반면 대엽형 Regal은 포복경장과 분지경수가 가장 적었고 증가도 완만하였다. 또한 포복경장은 예취간격을 줄임으로써 감소하였다.

• 한국초지조사료학회지
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• 제11권4호
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• pp.199-202
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• 1991

Red clover, ladino clover, white clover, alfalfa, 차풀 그리고 갈키나물등 6종의 콩과식물에서 잎과 줄기의 Esterase Isozyme를 추출하여 starch gel 전기영동법으로 분리한 후 염색하여 종간 차이점을 고찰하였다. 공시된 식물의 Esterase Isozyme의 band수, 염색강도, 이동속도는 종간에 차이가 있었다. 그러나 동일종에 있어서는 alfalfa를 제외하고는 잎과 줄기사이에 band의 수는 차이가 없었으며 차풀과 갈키나물에서는 전혀 나타나지 않았다.(Fig. 2). Esterase Isozyme의 염색강도와 이동속도는 Est 1 이 가장 강하고 빨랐다.

• The Plant Pathology Journal
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• 제33권4호
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• pp.424-428
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• 2017

Tomato yellow leaf curl virus (TYLCV) infection of the common bean (Phaseolus vulgaris) has been reported, but soybean (Glycine max) has not previously been identified as a TYLCV host. Five cultivars of white soybean were agro-inoculated using an infectious TYLCV clone. At 30 days post-inoculation, they showed infection rates of 25% to 100%. Typical TYLCV symptoms were not observed in any inoculated plants. To examine whether TYLCV was transmitted in soybean seeds, DNA was isolated from bundles of five randomly selected seeds from TYLCV-inoculated soybean plants and amplified with a TYLCV-specific primer set. With the exception of one bundle, all bundles of seeds were verified to be TYLCV-infected. Virus dissemination was also confirmed in three of the 14 bunches. Viral replication was also identified in seeds and seedlings. This is the first report demonstrating that soybean is a TYLCV host, and that TYLCV is a seed-transmissible virus in white soybean.