• Asian-Australasian Journal of Animal Sciences
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• v.20 no.3
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• pp.393-398
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• 2007

The present study was carried out to investigate the serum minerals profile in buffaloes (Bubalus bubalis) suffering from parturient haemoglobinuria (PHU) along with minerals profile of soils and fodders from the disease prone areas and their interrelationships. Serum samples were collected from 60 each of healthy and PHU affected buffaloes randomly selected from field cases. Serum samples were collected from each animal. Fifty composite soil samples were collected where PHU was prevalent. Fifty samples of fodders including leaves and stems being fed to the diseased buffaloes were collected. The difference in the levels of calcium and potassium between upper and lower soil surface of disease prone areas under study were statistically non-significant. The mean values of phosphorous, copper, iron, selenium and molybdenum in upper soil surface were significantly (p<0.05) higher than in lower soil surface. None of the fodders offered to the diseased animals met the dietary requirements of phosphorus and copper whereas none of the fodders was deficient in potassium, iron and selenium rather were having excess of potassium, iron and selenium. The concentration of calcium was adequate in lucerne, berseem, sarson and sorghum, while maize, sugarcane and wheat straw did not meet the required levels for dairy animals. Molybdenum contents in all fodders were adequate to meet the dietary requirements of the dairy buffaloes. Serum phosphorus, copper and selenium were significantly (p<0.001) lower whereas potassium, iron and molybdenum in buffaloes suffering from PHU were significantly (p<0.001) higher than in healthy buffaloes. It was concluded that phosphorous deficient soils play a major role by transferring this deficiency to plants and ultimately reaching to animals where hypophosphataemia is a consistent finding.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.229-237
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• 1987

In order to study the biological control of soil-borne disease of sesame, antagonistic isolates of Trichoderma , Bacillus sand streptomyces to Fusarium oxysporum and Rhizoctonia solani were isolated from the rhizosphere soils of sesame plants and some other habitats. Out of the isolates of microorganisms collected a strain of Trichoderma viride was selected as a biological control agent for the study and its effect on the control of damping-off and the seedling growth of sesame was investigated. The results obtained are as follows: 26 percents of Bacillus spp. isolated from the rhizosphere soil of sesame plants showed antagonism to two pathogenic fungi. Important species were B. Subtilis and B. polymyxa. Streptomyces species isolated from the rhizosphere soils of sesame lysed the cell wall of hyphae and conidia of F. oxysporum and reduced conspicuously the formation of macroconidia and chlamydospores of the fungus. 84 percents of Trichoderma spp. isolated from the rhizosphere soil of sesame plants were antagonistic to F. oxysporum and 60 percents of the isolates were antagonistic to both F. oxysporum and R. solani. Trichoderma viride TV-192 selected from antagonistic isolates of Trichoderma spp. was highly antagonistic to F. oxysporum and soil treatment with the isolate reduced notably damping-off of sesame. T. viride TV-192 showed better growth in crushed rice straw, barley straw and sawdust media than F. oxysporum. Sawdust was selective for the growth of T. viride. Supplementation of wheat bran and mixtures of wheat bran and sawdust inoculated with T. viride TV-192 in the soil reduced remarkably damping-off of sesame by F. oxysporum but high density of the fungus TV-192 caused the inhibition of seed germination and seedling growth of sesame. Inhibitory effects of Trichoderma species on seed germination and seedling growth of sesame were different according to the isolates of the fungus. Normal sesame seedlings on the bed treated with the fungus showed better growth than not treated seedlings.

• Journal of the Korean Professional Engineers Association
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• v.2 no.5
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• pp.4-18
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• 1969

This experiment was conducted, making use of the "NONG-RlM 6", a recommended variety of rice for year of 1968. Main purposes of the experiment are to explore possibilities of: a) ways and means of saving irrigation water and, b) overcoming drought at the same time so that an increased yield in rice could be resulted in. Specifically, it was tried to determine the effects of the circulation irrigation method combined with differentiated thickness of lining upon the growth and yield of rice. Some of the major findings are summarized in the following. 1) The different thicknesses show a significant relationship with the weight of 1,000 grains. In the case of 9cm-lined plot, the grain weight is 23.5 grams, the heaviest. Next in order are 3cm-lined plot, 6cm-lined plot, control plot, and wheat straw lined-plot. 2) In rice yield, it is found that there is a considerably moderate significant relationship with both the different thickness of lining and the number of irrigation, as shown in the table. 3) There is little or no difference among different plots in terms of a) physical and chemical properties of solid, b) quality of irrigation water, c) climatic conditions, and rainfalls. 4) It is found that there is a significant relationship between differences in the method of rotation irrigation and the number of ears per hill. The plot irrigated at an interval of 7 days shows 17.4 ears and the plot irrigated at an interval of 6 days, 16.3. 5) In vinyl-treated plots, it is shown that both yield and component element are greatest in the case of the plot with hole of 3$cm/m^2$. Next in order are; the plot with a hole of 2$cm/m^2$; the plot with a hole of 1$cm/m^2$. In the case of the plot with no hole, it is found that both yield and component elements are decreased as compared to the control plot. 6) The irrigation water requirement is measured for the actual irrigation days of 72 which are the number subtracted the days of rainfall of 30 from the total irrigation days of 102. It is found that the irrigation water requirement for the un control plot is 1,590 mm, as compared to 876 mm (44.9% saved) for the 9cm-lined plot, 959mm(39.7% saved) for the 6cm-lined plot, 1,010mm (36% saved) for the 3cm-lined plot, and 1,082mm (32% saved) for the wheat straw lined plot. In the case of the rotation irrigation method, it is found that the water requirement for the plot irrigated at an interval of 8 days is 538mm (65.3% saved), as compared to 617mm (61.1% saved) for the plot irrigated at an interval of 7 days, 672mm (57.7% saved) for the plot irrigated at an interval of 6 days, 746mm (53.0% saved) for the plot irrigated at an interval of 5 days, 890mm (44.0% saved) for to plot irrigated at an interval of 4 days, and 975mm (38.6% saved) for the plot irrigated at an interval of 3 days. 7) The rate of evapo-transpiration is found 2.8 around the end of the month of July, as compared to 2.6 at the begining of August, 3.4 around the end of August, and 2.6 at the begining of September. 8) It is found that the saturaton quantity of 30 mm per day is decreased to 20mm per day through the use of vinyl covering. 9) The husking rate shows 75 per cent which is considered better.

• Journal of Life Science
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• v.21 no.10
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• pp.1460-1465
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• 2011

This study was conducted to determine the effects of total mixed fermentation (TMF) with bean curd dregs on body weight gain, feed intake and body size of growing Hanwoo heifers. Thirty Hanwoo heifers, 6 months of age and weighing $125.9{\pm}12.0$ kg, were randomly allocated to 3 experimental groups of 10 animals each for a 6-month feeding trial. The groups were T1 (rice straw offered ad libitum with restricted (1.7% BW/day) concentrate), T2 (TMF only offered ad libitum) and T3 (TMF and rice straw offered ad libitum with restricted (1.0% BW/day) concentrate). TMF feeds were composed of 60% bean curd dregs, 16.5% corn meal, 10.4% wheat bran, 5% rice straw, 7% rice hulls, 0.5% urea matter, 0.5% magnesia lime and 0.1% lactic acid bacteria. The chemical composition and pH value of TMF feeds were measured at 0, 24, 48 and 72 hr after storage. In TMF with bean curd dregs, there were no differences in concentrations of DM, EE, and CF according to storage periouds. The ADF and NDF at 72 hr of storage time showed a significant decrease (9.52 and 3.15 %, respectively) compared to 0h of storage time (p<0.05). The value of pH was gradually decreased during the storage periods. Body weight gain, feed intake and average daily gain were higher in the TMF groups (T2, T3) compared to the T1 group (p<0.05). Changes in body size were also higher in the TMF groups compared to the T1 group (p<0.05). Thus, the present results indicate that TMF with bean curd dregs may help improve growth performance in growing Hanwoo heifers.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.179-185
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• 1988

Present study was undertaken in order to evaluate the lead contents of various feedstuffs produced in Korea and the effect of the burning of gasoline by automobiles on the lead contents. Ninety-one samples near and 95 far road were collected from 9 regions(Do). All of the samples were washed, dry-ashed and analyzed for lead content using atomic absorption spectrophotometer. The mean values obtained were checked for significance of difference using computer. The results obtained were summerized as follows: 1. The lead content in ${\mu}g/g$ of agriculture-byproducts were measured with the following mean values and ranges(in parentheses). Rice bran: $2.07{\pm}1.67$ (0-5.00), rice hull $1.84{\pm}1.18$ (0-3.71), rice straw, $1.27{\pm}1.09$ (0-3.62), soybean pod:$4.55{\pm}3.13$ ($1.13{\pm}10.43$), and wheat bran: $1.69{\pm}0.86$ (0-3.65). 2. The lead content in ${\mu}g/g$ of pasture plants were measured with the following mean values and ranges. Kudzu:$2.71{\pm}1.74$ ($0.38{\pm}8.75$), leed: $2.03{\pm}1.61$ (0-4.54), lespedeza:$4.73{\pm}5.93$ (0-31.25), sagebrush: $4.08{\pm}3.75$ (0-12.18), Korean-grass: $2.11{\pm}1.38$ (0-5.50), maizestalk: $2.62{\pm}2.35$ (0-8.65), clover: $3.83{\pm}2.97$ (0-12.19), alfalfa: $7.14{\pm}0.97$ (4.46-9.25), and timothy: $5.86{\pm}4.79$ (0-9.25). 3. Fourteen samples (22.2%) out of 63 agriculture-byproduct samples and 42 (34.2%) out of 123 pasture plant samples showed lead content of more than $3{\mu}g/g$ which are said to be the upper limit value for cattle. 4. No significant difference between the mean lead contents of near road, and those of far road was recorgnized. 5. The regional mean values for all kinds of samples collected in Kyungbuk and Kyungnam were significantly (p<0.05, p<0.10) higher compared to those in Chungnam and Jejudo.6. From these results it may be concluded that the burning of gasoline by automobiler did not affect on the lead contentes of washed feedstuffs produced in Korea at present.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.10
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• pp.1454-1464
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• 2015

Four methods were adopted, including the mobile nylon bag (MNB) method, modified three-step in vitro (MTS) method, original three-step in vitro (OTS) method, and acid detergent insoluble nitrogen (ADIN) estimating method, to evaluate the intestinal digestibility of rumen undegradable protein (DRUP) of 10 types of concentrates and 7 types of roughages. After correlation analysis to determine the DRUP values using the MNB, MTS, OTS, and ADIN methods, the study aimed to find out appropriate methods to replace the MNB method due to its disadvantages such as high price, long time period, and use of a duodenal T-fistula. Three dairy cows with a permanent ruminal fistula and duodenal T-fistula were used in a single-factor experimental design. The results showed that the determined DRUP values using the MNB method for soybean meal, cottonseed meal, rapeseed meal, sunflower meal, corn germ meal, corn, rice bran, barley, wheat bran, corn fiber feed, Alfalfa (Zhao dong), Alfalfa (Long mu 801), Alfalfa (Long mu 803), grass (North), Grass (Inner Mongolia), corn silage and corn straw were 98.13%, 87.37%, 88.47%, 82.60%, 75.40%, 93.23%, 69.27%, 91.27%, 72.37%, 79.03%, 66.72%, 68.64%, 73.57%, 50.47%, 51.52%, 54.05%, and 43.84%, respectively. The coefficient of determination ($R^2=0.964$) of the results between the MTS method and the MNB method was higher than that ($R^2=0.942$) between the OTS method and the MNB method. The coefficient of determination of the DRUP values of the concentrates among the in vitro method (including the MTS and OTS methods) and the MNB method was higher than that of the roughage. There was a weak correlation between the determined DRUP values in concentrates obtained from the ADIN method and those from the MNB method, and there was a significant correlation (p<0.01) between the determined DRUP values of the roughage obtained from the MNB method and those obtained from ADIN method. The DRUP values were significantly correlated with the nutritional ingredients of the feeds. The regression equation was DRUP =100.5566+0.4169CP - 0.4344SP - 0.7102NDF - 0.7950EE ($R^2=0.8668$, p<0.01; CP, crude protein; SP, soluble protein; NDF, neutral detergent fiber; EE, ether extract). It was concluded that both the MTS method and the OTS may suitable to replace the MNB method for determining the DRUP values and the former method was more effective. Only the ADIN method could be used to predict the values of the roughages but conventional nutritional ingredients were available for all of the samples' DRUP.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1547-1551
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• 2011

Bakery waste (BW) is much cheaper than barley (20 to 40% the price of barley). Bakery waste and barley grain have similar chemical composition; they contain 99 and 97% organic matter (OM), 1.1 and 1.8% fat, 18 and 15% neutral detergent fibre (NDF) and 14.0 and 14.5% crude protein (CP), respectively (DM basis). The objectives of this study were to determine the effect of substituting BW for barley grain in high concentrate fattening diets for lambs on nutrient intake, growth and carcass characteristics. Forty Awassi lambs (21.75${\pm}$1 kg) weaned at the age of 65 days were assigned randomly to four experimental fattening diets differing in BW ratio in a completely randomized design. The control diet (CON) contained 20, 60, 11, 7, and 2% (DM basis) wheat straw, barley grain, soybean meal, corn grain, and minerals and vitamin mix, respectively. Bakery waste substituted barley grain by 10, 20 and 30% of the diet DM in the LBW, MBW and HBW diets, respectively. The experiment lasted for 56 days. Dry matter intake (DMI) decreased (p<0.05) in LBW diet compared to the CON diet by approximately 10%. No further reduction in DMI was observed with the higher substitution levels. Metabolizable energy intake for the CON diet (3.6 Mcal/d) was also reduced (p<0.05) compared with LBW, MBW and HBW diets (3.4, 3.4 and 3.3 Mcal/d, respectively). Final body weight for lambs fed the CON diet (34.8 kg) was higher (p<0.05) compared with lambs fed the LBW, MBW and HBW diets (30.6, 32.0 and 31.1 kg, respectively). Growth rate for lambs fed the CON diet (232 g/d) was also higher (p<0.05) compared to lambs fed the LBW, MBW and HBW diets (170, 189, and 167 g/d, respectively). Feed to gain ratio was higher (p<0.05) for lambs fed the LBW, MBW and HBW diets (7.2, 6.6 and 7.3, respectively) compared with lambs that consumed the CON diet (5.7). Body weight gain cost was reduced by approximately 8% by the MBW and HBW diets as compared with the CON diet. Dressing percentage, full gut weight, empty gut weight and liver weights were all unaffected by the BW addition to the diets and averaged 48.9%, 6.8 kg, 2.8 kg and 0.444 kg, respectively. However, fat tail weight was increased (p<0.05) with the higher levels of the BW inclusion. In conclusion, substituting BW for barley grain reduced DMI and growth performance. However, when BW substituted barley grain at the 20 and 30% of the diet DM, body weight gain cost was reduced by approximately 8%.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.3
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• pp.188-192
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• 1990

Sixteen amino acids in the hydrolysates of fulvic acid fraction from 7 plant materials were determined. Analyzed amino acids were aspartic acid, glutamie acid, arginine, histidine, lysine, glycine, alanine, valine, leucine, isoleusine, phenylalanine, tyrosine, serine, threonine, proline, and methionine. Four crop residues, wild grass cuttings and forest tree litters were put under investigation. 1. The content of amino acids in fulvic acid fractions extracted after 90 days of compositing ranged from 0.15% to 0.53% by dry weight. The highest value was found in the fulvic acids of wild grass cuttings and the lowest in those of wheat straw, being equivalent to 1/5-1/31 of those found in humic acids. 2. The group of neutral amino acids shared the largest portion followed by acidic and basic amino acids. 3. Arginine was not detected in fulvic acid fractions from well decomposed residues. 4. Aromatic amino acids, phenylalanine and tyrosine, were virtually absent in fulvic acid fractions. 5. Glycine, glutamic acid and aspartic acid were the 3 major amino acids contained in fulvic acids of well decomposed residues. With glutamic acid and aspartic acid excluded, the decreasing order of concentration of amino acids was roughly in parallel with the increasing order of molecular weight.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1539-1545
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• 2007

An experiment was conducted using 20 male buffalo calves to study the effect of vitamin E and selenium supplementation on their immune response and plasma ${\alpha}$-tocopherol and selenium status. These buffalo calves (10-12 months old, average body weight $75.30{\pm}2.20 $ kg) were randomly allotted to four treatments on the basis of their body weights and were fed on wheat straw and concentrate mixture to meet their nutrient requirements of 500 g/d body weight gain. The buffalo calves were fed either a control diet (neither supplemented with Se nor VE) or diets supplemented with Se at 0.3 ppm (+Se), DL-alpha tocopheryl acetate at 300 IU (+VE), and both DL-alpha tocopheryl acetate at 300 IU and Se at 0.3 ppm (+Se+VE). These experimental diets were fed for 180 days. Blood samples were collected at day 0 and subsequently at 45 day intervals up to 180 days of experimental feeding to monitor plasma ${\alpha}$-tocopherol and Se concentrations. To assess humoral immune response, all calves were sensitized with formalin inactivated Pasteurella multocida antigen at 135 days of experimental feeding and blood was collected on 0, 7, 14, 21 and 28 days post vaccination (DPV) to measure antibody production using indirect ELISA. Cell mediated immune response of calves was assessed after 180 days of experimental feeding by in vivo delayed type hypersensitivity (DTH) reaction using phytohaemaglutinin-P (PHA-P) as a mitogen. Results revealed that feeding of VE and Se improved the plasma levels of these nutrients. Plasma levels of Se were affected by supplementation of both VE (p<0.001) and Se (p<0.001); however, no interaction ($Se{\times}VE$) was observed. Supplementation of Se improved the humoral immune response (p<0.008), whereas, VE showed a tendency towards improvement in cell mediated immune response (p<0.064). It was concluded that vitamin E and Se supplementation improved the status of these micronutrients and humoral immune response in buffalo calves.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1567-1574
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• 2007

A study was carried out to study the response of total purine derivatives (PD) excretion in urine to determine microbial N (MN) supply at four fixed levels of feed intake (namely 95, 80, 60 and 40% of voluntary intake). The crossbred (CB) calves were allocated according to a $4{\times}4$ Latin Square Design and fed wheat straw and concentrate (1:1). The rate of PD excretion (mmol/d) as a linear function of feed intake was 15.85/kg DMI and 20.12/kg DOMI. Based on the endogenous and PD excretion rates obtained in this study, a relationship between daily urinary PD excretion (Y, mmol) and daily microbial protein supply (X, mmol) was developed for crossbred calves as Y = 0.83X+0.296 kg $W^{0.75}$. The derived microbial N values using this equation differed (p<0.001) among the 4 groups and was the highest in L-95 followed by L-80, L-60 and L-40. The relationship between urinary nitrogen loss (Y, g/d) and DOMI (X, kg/d) was established as: Y = 6.038X+21.753 ($r^2$ = 0.663, p<0.01). When urinary excretion of PD (Y, mmol/d) was plotted against intake of DM and DOM (X, kg/d), the equations obtained were: Y = 7.1711X+8.674 ($r^2$ = 0.889, p<0.01) and Y = 12.434X+7.683 ($r^2$ = 0.896, p<0.01), respectively. The proportional contribution of allantoin and uric acid to total PD remained stable irrespective of level of feed intake. Similarly, urinary excretion of creatinine did not differ (p>0.05) between animals fed at different levels. The MN supply was the highest to animals at intake levels L-95, and decreased linearly with corresponding decrease in feed intake. However, the MN supply when expressed per kg DOMI remained statistically (p>0.05) similar irrespective of level of intake. The results revealed that the excretion of urinary purine derivatives were positively correlated with the level of feed intake as well as rumen microbial supply and thus it could be a good indicator for measuring the microbial protein supply and nutritional status of animals.