• 한국식품과학회지
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• 제30권6호
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• pp.1295-1300
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• 1998

산성, 중성 및 알칼리성 영역에서 각각 추출된 SPI를 첨가시킨 복합분의 제빵적성에 미치는 영향을 조사하였으며 제빵적성은 빵의 용적과 조직감을 측정하여 판정하였다. Mixograph를 이용하여 측정된 SPI-밀가루 복합분 반죽의 물리적 성질에서 pH 2.0과 12.0에서 추출된 SPI를 첨가한 복합분 반죽의 수분흡수율이 100% 밀가루 반죽의 수분흡수율보다 컸으며 SPI-밀가루 복합분의 반죽형성시간은 100% 밀가루 반죽에 비해 SPI의 종류에 관계없이 모두 감소하였다. 5% SPI-빵의 용적은 100% 밀가루로 만들어진 빵과 차이가 없었으며, 10% SPI-빵의 경우 $SPI_2$와 $SPI_3$-빵의 용적은 대조구보다 감소하였으나 $SPI_7,\;SPI_{10}$ 및 $SPI_{12}$-빵의 용적은 차이가 없었다. 또한 유화형성력이 큰 $SPI_7,\;SPI_{10},\;SPI_{12}$-빵의 용적이 유화형성력이 작은 $SPI_2,\;SPI_3$-빵 보다 크게 나타났다. 5% SPI-빵의 탄성, 씹힘성, 응집성, 껌성, 부착성 및 견고성은 대조구와 큰 차이를 나타내지 않았으며, 10% SPI-빵의 경우 $SPI_2$와 $SPI_{12}$-빵의 씹힘성, 껌성, 견고성에 있어 유의차를 보이며 증가하였고(P< 0.05) 대조구에 비해 전체적으로 큰 차이를 보이지 않았다.

• 농업과학연구
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• 제45권4호
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• pp.665-675
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• 2018

A total of 80 pigs [(Landrace ${\times}$ Yorkshire) ${\times}$ Duroc] with an average body weight of $72.9{\pm}2.6kg$ were used in the present study to investigate the effects of fermented whole crop wheat and barley with or without supplementing inoculums throughout the restricted feeding in finishing pigs. There were 4 replicate pens per treatment. Pigs were fed ad libitum throughout the experiment as the control (CON), and the other four groups were restricted to 10% in the CON diet and fed ad libitum fermented whole crop cereals: fermented whole crop barley with inoculums; fermented whole crop barley without inoculums; fermented whole crop wheat with inoculums; and fermented whole crop wheat without inoculums. During the entire experiment, the average daily feed intake (ADFI) decreased in the fermented barley and fermented wheat groups compared to the CON, while no difference was observed in the average daily gain (ADG), feed efficiency (gain : feed ratio, G : F) between the control and fermented whole crop barley, wheat diet group. Dry matter and nitrogen digestibility did not show a significant difference among the treatments. In the blood constituents, concentrations of blood urea nitrogen were significantly lower in pigs fed fermented whole crop barley without inoculum diets compared with the other treatments. In conclusion, restricted feeding with fermented whole crop barley and wheat regardless of the supplementing inoculums showed no significant difference in growth performance compared to the CON. This suggests that there is a possibility that fermented whole crop barley and wheat could replace part of the conventional diets.

• 한국식품조리과학회지
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• 제29권1호
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• pp.29-36
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• 2013

The present study was conducted to supply more scientific information for standardization of preparation method of Gyungdan and to determine the standard for manufacturing procedure adapted with mechanically impacting technology (MIT) for mass production. The optimum preparation condition for non-waxy Gyungdan adapted with MIT was 35% of water, 0.5% of wheat flour (w/w of soaked rice), and mechanically impacting for 10 min at 450 rpm. In the present study, standardization of manufacturing procedure for merchandizing of Gyungdan was established with width, weight, and height. Average of Gyungdan adapted with MIT at 90 rpm (stuffing speed) and 46.58 Herz (cutting speed) is 18.3 g of weight, 28.4 mm of height, and 32.4 mm of width. Hardness, as a main parameter of texture profile, of Gyungdan was maintained lower than 100 g up to 3 days at the storage of $20^{\circ}C$ and up to 70 days at the storage of $-20^{\circ}C$. And, it was performed to develop rice cake as meal-replacement adapted with MIT and sub-ingredients such as strawberry, pumpkin, and mugwort. Gyungdan prepared under controlling manufacturing condition of MIT was supplemented with 0-1.6% of sub-ingredients, respectively. In sensory tests, the best substitute ratios of strawberry, pumpkin, and mugwort were 1.6%, 0.8%, and 1.6%, respectively. With the results above, not-harden Gyungdan supplemented with sub-ingredients and various stuffs can be expected to application for wheat-substitute meal-replacement.

• 한국작물학회지
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• 제65권4호
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• pp.365-376
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• 2020

미성숙배를 이용한 조직배양은 높은 재분화율로 인하여 밀 형질전환에 적용하기 위한 가장 좋은 방법이지만 매우 한정적인 수집시기와 생육조건으로 인해 이용하기 위한 시간의 제한과 많은 노동력이 집약되어있다. 따라서 안정적이고 지속적인 미성숙배 수집을 위해 스피드 브리딩 조건을 이용하여 출수가 촉진된 밀 이삭을 이용한 미성숙배 조직배양의 효율을 검정하였고, 연구결과는 다음과 같다. 1. 스피드 브리딩 조건의 출수기는 정상조건과 비교하여 36일에서 54일까지 단축되었으며, 개화 후 11일 된 영과에서 수집된 미성숙배 조직배양의 재분화율과 소식물체 출현 비율은 정상조건과 비교하여 유의한 차이가 없다는 것을 확인하였다. 2. 두 배양 조건에서 항산화 효소 활성 분석 결과 차이가 나타나지 않았으며, 연관 표지 유전자 발현 검정을 통하여 일장과 세포분열과의 관계를 검정하여 스피드 브리딩 조건에서 수집된 이삭의 안정성을 검정하였다. 3. 본 연구 결과는 스피드 브리딩 조건을 활용한 미성숙배 조직배양에 활용될 것이며 관련 유전자 발현 및 항산화 활성 검정 결과를 통해 검증된 안정적 미성숙배는 밀 형질전환 기술 개발에 활용될 수 있을 것으로 사료된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2009년도 추계학술발표회
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• pp.48-48
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• 2009

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2000년도 국제심포지엄 및 제26차 추계학술발표회
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• pp.119-119
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• 2000

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 춘계학술발표회
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• pp.14-14
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• 2010

Plastid proteomics are essential organelles present in virtually all cells in plants and green algae. Plastids are responsible for the synthesis and storage of key molecules required for the basic architecture and functions of plant cells. The proteome of plastid, and in particular of chloroplast, have received significant amounts of attention in recent years. Various fractionation and mass spectrometry (MS) techniques have been applied to catalogue the chloroplast proteome and its sub-organelles compartments. To better understanding the function of the lumenal sub-organelles within the thylakoid network, we have carried out a systematical analysis and identification of the lumenal proteins in the thylakoid of wheat by using Tricine-SDS-PAGE, and LTQ-ESI-FTICR mass spectrometry followed by SWISS-PROT database searching. We isolation and fractionation these membrane from fully developed wheat leaves using a combination of differential and gradient centrifugation couple to high speed ultra-centrifuge. After collecting all proteins to eliminate possible same proteins, we estimated that there are 407 different proteins including chloroplast, chloroplast stroma, lumenal, and thylakoid membrane proteins excluding 20 proteins, which were identified in nucleus, cytoplasm and mitochondria. A combination of these three programs (PSORT, TargetP, TMHMM, and TOPPRED) was found to provide a useful tool for evaluating chloroplast localization, transit peptide, transmembranes, and also could reveal possible alternative processing sites and dual targeting. Finally, we report also sub-cellular location specific protein interaction network using Cytoscape software, which provides further insight into the biochemical pathways of photosynthesis. The present work helps understanding photosynthesis process in wheat at the molecular level and provides a new overview of the biochemical machinery of the thylakoid in wheat.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 춘계학술대회
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• pp.132-132
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• 2019

• 한국식품과학회지
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• 제7권2호
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• pp.96-99
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• 1975

쌀보리의 합리적인 제분 방법을 규명하고저 tempering 최적조건 부분 도정후의 제분성, 밀과의 혼합 제분성 등을 test mill 을 이용, stock stream 별로 검토해 본 결과 다음과 같은 결과를 얻었다. 1. 쌀보리의 최적 tempering 조건으로서는 수분 13. 5%에서 48시간 일차 tempering 을 한 후 이어 제분 30분전 0. 5% 가수 처리하는 것이 가장 유리하였다. 2. tempering 전후 부분 도정을 실시하므로써, 회분 함량은 저하시킬 수 있었으나 수율이 크게 저하되고, 부분도정 후 제분이란 두 단계의 가공 과정이 필요하므로 비효율적이었다. 3. 쌀보리를 밀과 혼합하여 제분할 때, 쌀보리의 혼합비율은 $10{\sim}20%$가 합리적이며, 이때 사용되는 원료 밀은 연질보다 경질의 밀이 더욱 유리하였다.

• Fisheries and Aquatic Sciences
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• 제18권2호
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• pp.159-164
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• 2015

A feeding experiment was conducted to determine the effect of dietary distillers dried grain (DDG) on the growth and body composition of juvenile abalone, Haliotis discus hannai. Three diets were formulated to contain 0% (DDG0), 30% DDG (DDG1) replacing wheat flour and 30% DDG (DDG2) replacing fish meal and wheat flour. A commercial feed (CF) was also compared with experimental diets. Three replicate groups of abalone averaging $0.6{\pm}0.01g$ were fed one of the four diets for 14 weeks. The highest survival rate was observed in the abalone fed the DDG2 diet, which was higher than that of abalone fed the DDG0 diet. Weight gain, shell length, shell width and soft body weight of the abalone were not affected by dietary DDG and CF diet (P > 0.05). Proximate and amino acid composition of the soft whole body were not affected by dietary DDG and CF diet (P > 0.05). The result of this experiment suggests that DDG is a good ingredient to replace fish meal and wheat flour in the diet and could be used up to 30% in the diet without negative effects on the growth performance of juvenile abalone Haliotis discus hannai.