• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.1980-1985
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• 2007

The purpose of this study is to investigate the performance of an autocascade refrigeration system using the refrigerant mixtures of R-134a(64${\sim}$72wt%), R-23(20${\sim}$25wt%) and R-14(8${\sim}$12wt%) as working fluids by experiment. The experimental apparatus consisted of a autocascade system with a compressor, condenser, evaporator, gas-liquid separator and cascade condenser, etc. Two main causes to this failure were considered as follows. First, the pressure ratio in the compressor was extremely increased at the beginning of the experiment. Second, the outlet temperature in compressor was higher than its limited temperature. For a stable operation of the compressor, a portion of the mixed refrigerant was by-passed at the inlet of the heat exchanger and transferred directly to the suction of the compressor in the modified system. The experimental parameters were changed weight of R-134a(1800g, 2800g), R-23(700, 800, 900g) and R-14(300, 400, 500g). The results showed that the best performance in case of R-134a of 2800g, R-23 of 900g and R-14 of 500g.

• 한국표면공학회지
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• 제42권6호
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• pp.280-286
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• 2009

In this study, the corrosion property of copper alloy tubes in seawater has been investigated. Three copper alloys of nominal composition Cu-20Zn-2Al(Al-Brass), Cu-30Ni(CN70/30) and Cu-10Ni(CN90/10) were considered. The samples were immersed in 3%NaCl flowing solution at $90^{\circ}C$ for 30, 50 and 80 days. Corrosion rate of copper alloy tubes in 3%NaCl flowing solution was investigated by weight-loss measurements and electrochemical test. The CN70/30 showed lowest corrosion rate among three copper alloy tubes. Because of passive films formation, corrosion rates of three types of copper tubes were decrease with time. Surface characteristics of copper alloy tubes were analyzed by optical micrograph(OM), scanning electronic microscopy (SEM), energy dispersive X-ray analysis(EDAX) and X-ray diffraction patterns(XRD). CN70/30 showed partly pitting problem on the surface owing to high Fe content, even though having high resistant against corrosion. Cracks appeared on the surface of CN90/10 and CN70/30 after more than 50 days immersion, which could be derived from high nickel contents.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2005년도 제24회 춘계학술대회논문집
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• pp.168-175
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• 2005

액체로켓 추진시스템에서 가압시스템은 발사체 추진제 탱크의 얼리지 공간에 제어된 가스를 공급하는 것이다. 가압시스템에서 고온 가스 열교환기를 적용하는 데는 가압제의 비용적을 증가시켜 전체 발사체 시스템의 중량을 감소시키는 장점이 있다. 가압시스템 성능에 있어서 주목할 만한 개선점은 극저온 시스템에서 얻어질 수 있다. 이러한 경우 가스 공급은 극저온 탱크 내부에 저장되어 진다. 극저온 가압제의 온도 특성은 가압시스템에서 구성 단품을 개발하는데 있어서 매우 중요하다. 본 연구에서는 SINDA/FLUINT를 이용한 수치적 모델링과 PTF(Propellant-feeding Test Facility)에서 수행된 실험에 대하여 해석 및 시험이 수행되었다.

• Journal of Advanced Marine Engineering and Technology
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• 제32권2호
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• pp.315-322
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• 2008

Titanium and its alloys have excellent corrosion resistance, high strength to weight ratios and creep properties in high temperature, which make them using many various fields of application. Especially, pure titanium, which has outstanding resistance for the stress corrosion cracking, crevice corrosion, pitting and microbiologically influenced corrosion, brings out to the best material for the heat exchanger, ballast tank, desalination facilities, and so on. Responding to these needs, welding processes for titanium are also being used GTAW, GMAW, PAW, EBW, LBW, resistance welding and diffusion bonding, etc. However, titanium is very active and highly susceptible to embrittlement by oxygen, nitrogen, hydrogen and carbon at high temperature, so it needs to shield the weld metal from the air and these gases during welding by non-active gas. In this study, it was possible to get sound beads without humping and spatter with a decrease of peak power according to increase of pulse width, change of welding speed and overlap rate for heat input control, and shield conditions at pulsed laser welding of titanium plates for Lap welding.

• BMB Reports
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• 제34권4호
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• pp.310-315
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• 2001

The uneven distribution of acidic and basic chitinases in different parts of rice seed, and also the characterization of hull-specific chitinases, are reported here. After extraction of chitinases from polished rice, bran, and rice hulls, the chitinases were separated into acidic and basic fractions, according to their behavior on an anion exchanger column. Both fractions from different parts of rice seed showed characteristic activity bands on SDS-PAGE that contained 0.01% glycol chitin. The basic chitinases from rice hulls were further purified using chitin affinity chromatography. The chitinase, specific to rice hulls (RHBC), was 88-fold purified with a 1.3% yield. RHBC has an apparent molecular weight of 22.2 kDa on SDS-PAGE. The optimal pH and temperature were 4.0 and $35^{\circ}C$, respectively. With [$^3H$]chitin as a substrate, RHBC has $V_{max}$ of 13.51 mg/mg protein/hr and $K_m$ of 1.36 mg/ml. This enzyme was an endochitinase devoid of ${\beta}$-1,3-glucanase, lysozyme, and chitosanase activities.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.205.2-205.2
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• 2014

The effects of ion beam mixing of a SiC film coated on super alloys (hastelloy X substrates) were studied, aiming at developing highly sustainable materials at above $900^{\circ}C$ in decomposed sulfuric acid gas (SO2/SO3/H2O) channels of a process heat exchanger. The bonding between two dissimilar materials is often problematic, particularly in coating metals with a ceramics protective layer. A strong bonding between SiC and hastelloy X was achieved by mixing the atoms at the interface by an ion-beam: The film was not peeled-off at ${\geq}900^{\circ}C$, confirming excellent adhesion, although the thermal expansion coefficient of hastelloy X is about three times higher than that of SiC. Instead, the SiC film was cracked along the grain boundary of the substrate at above $700^{\circ}C$. At ${\geq}900^{\circ}C$, the film was crystallized forming islands on the substrate so that a considerable part of the substrate surface could be exposed to the corrosive environment. To cover the exposed areas and cracks multiple coating/IBM processes have been developed. An immersion corrosion test in 80% sulfuric acid at $300^{\circ}C$ for 100 h showed that the weight retain rate was gradually increased when increasing the processing time.

• 한국추진공학회지
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• 제9권3호
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• pp.66-73
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• 2005

액체로켓 추진시스템에서 가압시스템은 발사체 추진제 탱크의 얼리지 공간에 제어된 가스를 공급하는 것이다. 가압시스템에서 고온 가스 열교환기를 적용하는 데는 가압제의 비체적을 증가시켜 전체 발사체 시스템의 중량을 감소시키는 장점이 있다. 가압시스템 성능에 있어서 주목할 만한 개선점은 극저온 시스템에서 얻어질 수 있다. 이러한 경우 가스 공급은 극저온 탱크 내부에 저장되어 진다. 본 연구에서는 극저온 추진제를 모의(模擬)하기 위하여 액체 질소를 사용하였다. 극저온 가압제의 온도 특성은 가압시스템에서 구성 단품을 개발하는데 있어서 매우 중요하다. 본 연구에서는 SINDA/FLUINT를 이용한 이론적 해석과 PTF에서 수행된 실험 결과에 대하여 비교 분석이 수행되었다.

• 농업과학연구
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• 제41권3호
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• pp.237-243
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• 2014

Human Glucagon like peptide-1 (GLP-1) is an incretin hormone that promotes secretion of insulin. In order to eliminate the formation of the soluble aggregate, Ala19 in GLP-1 was substituted with Thr, resulting in a GLP-1 mutant GLP-1A19T. The gene synthesis of GLP-1A19T and the fusion of 6-lysine tagged ubiquitin gene were accomplished by using the overlap extension polymerase chain reaction. The ubiquitin fused GLP-1A19T (K6UbGLP-1A19T) is expressed as form of inclusion body with little formation of the soluble aggregation in recombinant E. coli. In order to produce K6UbGLP-1A19T in large amounts, fed-batch fermentation was carried out in a pH-stat feeding strategy. Maximum dry cell weight of 87.7 g/L and 20.4% of specific K6UbGLP-1A19T content were obtained. Solid-phase refolding using a cation exchanger was carried out to renature K6UbGLP-1A19T. The refolded K6UbGLP-1A19T aggregated little and was released GLP-1A19T by on-column cleavage with ubiquitin-specific protease-1. The molecular mass of GLP-1A19T showed an accurate agreement with its theoretical molecular mass.

• Journal of Advanced Marine Engineering and Technology
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• 제20권3호
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• pp.91-100
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• 1996

Experimental results for forced convection condensation of non-azeotropic refrigerant mixtures inside a horizontal smooth tube are presented. The mixtures of R-22+R-134a and pure refrigerants R-22 and R-134a are used as the test fluids and a double pipe heat exchanger of 7.5mm ID and 4800mm long inside tube is used. The range of parameters are 100-300kg/h of mass flow rate, 0-1.0 of quality, and 0, 33, 50, 67, and 100 weight percent of R-22 mass fraction in the mixtures. The heat flux, vapor pressure, vapor temperature and tube wall temperature were measured. Using the data, the local and average heat transfer coefficients for the condensation have been obtained. In the same given experimental conditions, the liquid heat transfer coefficients for NARMs were considerally lower than that of the pure refrigerant of R-22 and R-134a. Local heat transfer characteristics for NARMs were different from pure refrigerant R-22 and R-134a. In some regions, local heat transfer coefficients for NARMs were increased in the following order ; Bottom$\rightarrow$Top$\rightarrow$Side. The condensation heat transfer coefficients for NARMs increased with mass velocity, heat flux, and quality, but were considerably lower than that of pure refigerant R-22 and R-134a.

• Parasites, Hosts and Diseases
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• 제46권3호
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• pp.183-186
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• 2008

Helminthic cysteine proteases are well known to play critical roles in tissue invasion, nutrient uptake, and immune evasion of the parasites. In the same manner, the sparganum, the plerocercoid of Spirometra mansoni, is also known to secrete a large amount of cysteine proteases. However, cysteine protease inhibitors regulating the proteolytic activities of the cysteine protease are poorly illustrated. In this regard, we partially purified an endogenous cysteine protease inhibitor from spargana and characterized its biochemical properties. The cysteine protease inhibitor was purified by sequential chromatographies using Resource Q anion exchanger and Superdex 200 HR gel filtration from crude extracts of spargana. The molecular weight of the purified protein was estimated to be about 11 kD on SDS-PAGE. It was able to inhibit papain and 27 kDa cysteine protease of spargana with the ratio of 25.7% and 49.1%, respectively, while did not inhibit chymotrypsin. This finding suggests that the cysteine protease inhibitor of spargana may be involved in regulation of endogenous cysteine proteases of the parasite, rather than interact with cysteine proteases from their hosts.