• The Korean Journal of Physiology and Pharmacology
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• v.1 no.3
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• pp.285-296
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• 1997

Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.

• Transactions of the Korean Society of Mechanical Engineers
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• v.13 no.1
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• pp.67-76
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• 1989

Although the standard 2-CR network filter, one of the filters used for measuring surface roughness, is being used widely in the field of industry it possesses a weak point of causing Phase-distortion when the wavelength is passing through. Phase-corrected filter is used for eliminating these phase-distortion and the difference of phase characteristics between these two filters is thought to have considerable amount of effect on the surface roughness value. Moveover, the definition on this is as yet unclear. Therefore, the Rt and Rz value, obtained when the wavelength are passed through the standard and phase-corrected filters from the same random machine-processed surface, and its standard deviation are compared and experimented in this paper. Also, the ratio of relative bearing curve complying to the phase characteristics of these two filters are acquired. As a result, the use of phese-corrected filter is appropriate in experiments where the form of wavelength is important, and the standard deviation on the same experimented values appeared greater with when using the standard filter. The rate of relative bearing curve became greater as .lambda.$_{c}$, the cut-off value of filter, became smaller.r.

• Korean Journal of Veterinary Research
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• v.36 no.1
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• pp.169-179
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• 1996

This study was carried out to examine the role of small cells and oval cells in cholangiocarcinogenesis in the hamsters infected with Clonorchis(C) sinensis. Forty two female Syrian golden hamsters were divided into two groups. Group I was for the induction of the cholangiocarcinoma, which was infected orally with C sinensis and given dimethylnitrosamine(15ppm) in drinking water for 4 weeks. Group II was served as control. More than 5 heads of hamsters in each group were sacrificed at 4, 7, 11 and 15 weeks after the beginning of the experiment. The livers were examined histopathologically, electron microscopically and immunohistochemically. The results obtained were as follows; 1. Cholangiocarcinomas were occurred in 1 of 6 animals at 11 weeks and in 4 of 6 animals at 15weeks after the beginning of the experiment. 2. Small cells and oval cells were proliferated around the portal triads from 4 weeks and peaked at 11 weeks, and slightly decreased after then. 3. The strong positive reaction to the $\alpha$-fetoprotein was shown in many of small cells and oval cells. But ductlike oval cells, which were arranged rosette form, showed week positive reaction to the $\alpha$-fetoprotein. 4. Most of small cells and oval cells showed negative reaction to the cytokeratin. But weak positive reaction in ductlike oval cells, and moderate positive reaction in cholangiocarcinoma cells were observed. These results suggested that cholangiocarcinoma induced by infection of C sinensis was believed to originate from the proliferated small cells around the portal triads which would be able to differentiate to the oval cells, ductlike oval cells, and cholangiocarcinoma cells gradually.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.2
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• pp.114-125
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• 2020

In order to understand the currents around Hampyung Bay and Haeje Peninsula, 2D numerical simulations for tidal currents and tide-induced residual currents were carried out. Dominant semidiurnal tidal currents have reversing form and flow NNE-SSW from northern Haeje Peninsula to Songi Island, E-S at northern Haeje Peninsula and NNW-SSE in Hampyung Bay. In flood, a part of currents from Imja Island~Nakwhol Island flow along the main stream flowing northeast at offshore region and the rest flow into Hampyung Bay flowing east along the northern coast of Haeje Peninsula. In ebb, currents from Hampyung Bay flow west along the northern coast of Haeje Peninsula and run together with the main stream flowing southeast at offshore region. The currents create an anticyclonic circulation in flood and a cyclonic circulation in ebb around Haeje Peninsula including Hampyung Bay. Tidal currents are accumulated on Doripo which located at the entrance of Hampyung Bay and show high current velocities. Tidal currents and tide induced residual currents are weak at the inside of Hampyung Bay which has narrow entrance, shallow water depth and wide intertidal zone. An anticyclonic eddy is formed around Gaksi Island as a result of tide induced residual currents. In northern coast of Haeje Peninsula, slow constant currents flow east. It is expected that a gradual change of sediment and an increase of flushing time for suspended materials are carried by tidal currents occurring in Hampyung Bay.

• Journal of Haehwa Medicine
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• v.15 no.2
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• pp.39-52
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• 2006

We came to the conclusion as follows from the research about pulse diagnostic technique (脈診) out of "Nan Jinghoeju jeonjung" (難經滙注箋正). 1. "Nan Jing" is practical medical book for clinician and holds different opinions about three portions and nine takings(三部九候脈法) from Huang Di Nei Jing's Taking the pulse of Bu Jung Chim Chon Kwan Chuk((浮中沈 寸關尺) is a quite creative means which has been a important role to future generation. 2. We pointed the differences between western medical science and traditional medical science. And can explain the pulse in western medicine divided from twelve channels of Oriental Medicine. We can explain special connection between heart and lungs using the theory of systemic & pulmonary circulation in western medicine. And this can be a basement of Lung controls every pulse theory in oriental medicine. 3. We have negative assertion about viewing the human body using theory of Yin and Yang-Five Elements. And have poor opinion of explaining about the matching internal organs to Chon Kwan Chuk using the theory of Yin and Yang-Five Elements. 4. We pointed out the mistake that Porak & Sang hwa act for heart. We took pulses considering heart is a real actual internal organ as others. 5. We denied the theory of Man's Chuk pulse is always weak(男子尺脈恒弱) & Woman's Chuk pulse is always strong(女子尺脈恒盛). The physical form of man and woman is not different from each other considering their inheritances from forefather & same shape of organs. So, we cannot insist on the theory.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.4
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• pp.493-499
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• 2007

The Taylor expansion expresses a differentiable function and its coefficients provide good approximations for the given function and its derivatives. In this study, m-th order Taylor Polynomial is constructed and the coefficients are computed by the Moving Least Squares method. The coefficients are applied to the governing partial differential equation for solid problems including crack problems. The discrete system of difference equations are set up based on the concept of point collocation. The developed method effectively overcomes the shortcomings of the finite difference method which is dependent of the grid structure and has no approximation function, and the Galerkin-based meshfree method which involves time-consuming integration of weak form and differentiation of the shape function and cumbersome treatment of essential boundary.

• Journal of Sasang Constitutional Medicine
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• v.21 no.1
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• pp.44-52
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• 2009

1. Objectives Jeong-Jo's death has many mistery. So we understand rightly Jeong-Jo's death. we inspect closely medical records of 20 days before death(in The Annals of the Choson Dynasty(朝鮮王朝實錄), Hong Je Jun Se(弘齋全書)， GukSoBoGam(國朝寶鑑). We understand medical treatment before death. It is based on Jeong-Jo's Constitution. So we trace the cause of a Jeong-Jo's death rightly. 2. Methods According to The Annals of the Choson Dynasty(朝鮮王朝實錄), Hong Je Jun Se(弘齋全書) GukSoBoGam(國朝寶鑑). We found out Jeoung-Jo's Sasang constitutional elemet. We point on Jeoung-Jo's nature and emotion, temperament and talent, features and way of speaking, physical appearance, healthy state, ordinary symptom, pathological syndromes and pharmacology. so documentary records was worthy of notice. 3. Results and conclusions 1. Jeong-Jo has prominent cheekbones,flat face. It belong to Tae-eum. 2. Jeong-Jo's physical appearance is mild,around,large. It belong ro Tae-eum. 3. Jeong-Jo's favorite food and herb were belong to Tae-eum interior febrile disease herb. which is Exhale Dispersing Qi 4. So even though Jeoung-Jo is Tae-eum, He had a weak body. It main cause that Smoking, insomnia, heave work, Hwa disease. 5. Jeong-Jo's is Interior febrile disease induced form the liver affected by heat in Tae-eumin

• The Journal of the Korea Contents Association
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• v.10 no.1
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• pp.46-58
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• 2010

The user valence of VoIP services with SIP protocol is increasing rapidly because of cheap communication cost and its conveniency. But attacker can easily modify the packet contents of SIP protocol as SIP header is transmitted by using UDP methods in text form. The reason is that SIP protocols does not provide an authentication function on the transmission session. Therefore, existing SIP protocol is very weak on SIP Packet Flooding attack etc. In order to solve like this kinds of SIP vulnerabilities, we used SIP status codes under the monitoring module for detecting SIP Flooding attacks and additionally proposed an advanced protocol where the authentication and security function is strengthened about SIP packet. We managed SIP session spontaneously in order to strengthen security with SIP authentication function and to solve the vulnerability of SIP protocol. The proposed mechanism can securely send SIP packet to solves the security vulnerability with minimum traffic transmission. Also service delay in SIP proxy servers will be minimized to solve the overload problem on SIP proxy server.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.6
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• pp.599-607
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• 2017

Most normal cells express L-type amino acid transporter 2 (LAT2). However, L-type amino acid transporter 1 (LAT1) is highly expressed in many tumor cells and presumed to support their increased growth and proliferation. This study examined the effects of JPH203, a selective LAT1 inhibitor, on cell growth and its mechanism for cell death in Saos2 human osteosarcoma cells. FOB human osteoblastic cells and Saos2 cells expressed LAT1 and LAT2 together with their associating protein 4F2 heavy chain, but the expression of LAT2 in the Saos2 cells was especially weak. JPH203 and BCH, a non-selective L-type amino acid transporter inhibitor, potently inhibited L-leucine uptake in Saos2 cells. As expected, the intrinsic ability of JPH203 to inhibit L-leucine uptake was far more efficient than that of BCH in Saos2 cells. Likewise, JPH203 and BCH inhibited Saos2 cell growth with JPH203 being superior to BCH in this regard. Furthermore, JPH203 increased apoptosis rates and formed DNA ladder in Saos2 cells. Moreover, JPH203 activated the mitochondria-dependent apoptotic signaling pathway by upregulating pro-apoptotic factors, such as Bad, Bax, and Bak, and the active form of caspase-9, and downregulating anti-apoptotic factors, such as Bcl-2 and Bcl-xL. These results suggest that the inhibition of LAT1 activity via JPH203, which may act as a potential novel anti-cancer agent, leads to apoptosis mediated by the mitochondria-dependent intrinsic apoptotic signaling pathway by inducing the intracellular depletion of neutral amino acids essential for cell growth in Saos2 human osteosarcoma cells.

• Asian Journal of Atmospheric Environment
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• v.2 no.1
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• pp.34-46
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• 2008

Emissions inputs for use in air quality modeling of Korea were generated with the emissions inventory data from the National Institute of Environmental Research (NIER), maintained under the Clean Air Policy Support System (CAPSS) database. Source Classification Codes (SCC) in the Korea emissions inventory were adapted to use with the U.S. EPA's Sparse Matrix Operator Kernel Emissions (SMOKE) by finding the best-matching SMOKE default SCCs for the chemical speciation and temporal allocation. A set of 19 surrogate spatial allocation factors for South Korea were developed utilizing the Multi-scale Integrated Modeling System (MIMS) Spatial Allocator and Korean GIS databases. The mobile and area source emissions data, after temporal allocation, show typical sinusoidal diurnal variations with high peaks during daytime, while point source emissions show weak diurnal variations. The model-ready emissions are speciated for the carbon bond version 4 (CB-4) chemical mechanism. Volatile organic carbon (VOC) emissions from painting related industries in area source category significantly contribute to TOL (Toluene) and XYL (Xylene) emissions. ETH (Ethylene) emissions are largely contributed from point industrial incineration facilities and various mobile sources. On the other hand, a large portion of OLE (Olefin) emissions are speciated from mobile sources in addition to those contributed by the polypropylene industry in point source. It was found that FORM (Formaldehyde) is mostly emitted from petroleum industry and heavy duty diesel vehicles. Chemical speciation of PM2.5 emissions shows that PEC (primary fine elemental carbon) and POA (primary fine organic aerosol) are the most abundant species from diesel and gasoline vehicles. To reduce uncertainties in processing the Korea emission inventory due to the mapping of Korean SCCs to those of U.S., it would be practical to develop and use domestic source profiles for the top 10 SCCs for area and point sources and top 5 SCCs for on-road mobile sources when VOC emissions from the sources are more than 90% of the total.