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Comparisons between Micro-Kjeldahl and Near Infrared Reflectance Spectroscopy for Protein Content Analysis of Malting Barley Grain (근적외분광분석법과 Micro-Kjeldahl 법 간의 맥주보리 종실의 단백질함량 분석 비교)

  • Kim, Byung-Joo;Suh, Duck-Yong;Suh, Hyung-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.5
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    • pp.489-494
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    • 1994
  • Near Infrared Reflectance Spectroscopy(NIRS) has been used as a tool for the rapid, accurate, protein assay of malting barley. NIRS used in this study was filter type instruments, Neotec 102. The objective of this study was to obtain the best calibration equation, for the rapid, ease and accurate protein content analysis of malting barley using NIRS system. The optimum wavelength for protein content analysis used NIRS were 2095nm, 2095/1941nm, 2095/1941/2282nm, 2905/1941/2282/2086nm, respectively. Mean protein content with this calibration equation in NIRS analysis was 10.59%, while 10.60% in Micro-Kjeldahl one. The range of protein content in Micro-Kjeldahl was 8.66~12.66% and that in NIRS was 8.80~12.35%. When 18 other varieties produced in 1992 were analysed with 2095nm, 2095/1941nm, 2095/1941/2282nm, 2095/1941/2282/2086nm equation, standard deviation of difference (SDD)and standard error of performence(SEP) and $R^2$ values were 0.47, 0.43, 0.95, respectively. Both the mean protein content by Micro-Kjeldahl and by NIRS was 10.25%. With this equation, analysied 31 varities produced in 1993, SDD and SEP and r values were 0.69, 0.67, 0.91, respectively, and that bias value was 0.65. In this analysis, mean protein content by Micro-Kjeldahl was 10.17% and by NIRS was 10.81%. The range of protein content in Micro-Kjeldahl was 7.58~14.29%, What that in NIRS was 8.63~13.93%. After adjusted bias in the best calibration equation, mean protein content of Micro-Kjeldahl was 10.17% and that of NIRS was 10.09%, without variance of SDD, SEP and r values.

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THE USE OF NEAR INFRARED REFLECTANCE SPECTROSCOPY(NIRS) TO PREDICT CHEMICAL COMPOSITION ON MAIZE SILAGE

  • D.Cozzolino;Fassio, A.;Mieres, J.;Y.Acosta
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1610-1610
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    • 2001
  • Microbiological examination of silage is of little value in gauging the outcome of silage, and so chemical analysis is more reliable and meaningful indicator of quality. On the other hand chemical assessments of the principal fermentation products provide an unequivocal basis on which to judge quality. Livestock require energy, protein, minerals and vitamins from their food. While fresh forages provide these essential items, conserved forages on the other hand may be deficient in one or more of them. The aim of the conservation process is to preserve as many of the original nutrients as possible, particularly energy and protein components (Woolford, 1984). Silage fermentation is important to preservation of forage with respect of feeding value and animal performance. Chemical and bacteriological changes in the silo during the fermentation process can affect adversely nutrient yield and quality (Moe and Carr, 1984). Many of the important chemical components of silage must be assayed in fresh or by extraction of the fresh material, since drying either by heat or lyophilisation, volatilises components such as acids or nitrogenous components, or effects conversion to other compounds (Abrams et al., 1987). Maize silage dorms the basis of winter rations for the vast majority of dairy and beef cattle production in Uruguay. Since nutrient intake, particularly energy, from forages is influenced by both voluntary dry matter intake and digestibility; there is a need for a rapid technique for predicting these parameters in farm advisory systems. Near Infrared Reflectance Spectroscopy (NIRS) is increasingly used as a rapid, accurate method of evaluating chemical constituents in cereals and dried forages. For many years NIRS was applied to assess chemical composition in dry materials (Norris et al., 1976, Flinn et al., 1992; Murray, 1993, De Boever et al., 1996, De la Roza et al., 1998). The objectives of this study were (1) to determine the potential of NIRS to assess the chemical composition of dried maize samples and (2) to attempt calibrations on undried samples either for farm advisory systems or for animal nutrition research purposes in Uruguay. NIRS were used to assess the chemical composition of whole - plant maize silage samples (Zea mays, L). A representative population of samples (n = 350) covering a wide distribution in chemical characteristics were used. Samples were scanned at 2 nm intervals over the wavelength range 400-2500 nm in a NIRS 6500 (NIRSystems, Silver Spring, MD, USA) in reflectance mode. Cross validation was used to avoid overfitting of the equations. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The calibration statistics were R$^2$ 0. 86 (SECV: 11.4), 0.90 (SECV: 5.7), 0.90 (SECV: 16.9) for dry matter (DM), crude protein (CP), acid detergent fiber (ADF) in g kg$\^$-1/ on dry matter, respectively for maize silage samples. This work demonstrates the potential of NIRS to analyse whole - maize silage in a wide range of chemical characteristics for both advisory farm and nutritive evaluation.

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Catadioptric NA 0.6 Objective Design in 193 nm with 266 nm Autofocus (이중 파장 심자외선 카타디옵트릭 NA 0.6 대물렌즈 광학 설계)

  • Do Hee Kim;Seok Young Ju;Jun Ho Lee;Hagyong Kihm;Ho-Soon Yang
    • Korean Journal of Optics and Photonics
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    • v.34 no.2
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    • pp.53-60
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    • 2023
  • We designed a catadioptric objective lens with a 0.6 numerical aperture (NA) for semiconductor inspection at 193 nm. The objective lens meets major requirements such as a spatial resolution of 200 nm and a field of view (FOV) of 0.15 mm or more. We selected a wavelength of 266 nm for autofocus based on the availability of the light source. First, we built the objective lenses of three lens groups: a focusing lens group, a field-lens group, and an NA conversion group. In particular, the NA conversion group is a group of catadioptric lenses that convert the numerical aperture of the beam focused by the prior groups to the required value, i.e., 0.6. The last design comprises 11 optical elements with root-mean-squared (RMS) wavefront aberrations less than λ/80 over the entire field of view. We also achieved the athermalization of the objective lens with focus-shift alone satisfying the performance of RMS wavefront aberration below λ/30 at a temperature range of 20 ± 1.2 ℃.

A Comparative Study of Sulfate and Chloride Intrusion in Mortar Sections: An Approach Using Laser Induced Breakdown Spectroscopy and Ion Exchange Membrane (LIBS와 이온교환막을 활용한 모르타르 단면 침투 황산염과 염화물 분석)

  • Park, Won-Jun
    • Journal of the Korea Institute of Building Construction
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    • v.23 no.3
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    • pp.221-229
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    • 2023
  • This research aimed to conduct an empirical assessment of the penetration of chloride and sulfate ions into mortar sections using an anion exchange membrane(AEM) and laser-induced breakdown spectroscopy(LIBS). The study involved a simultaneous ion chromatography(IC) analysis and LIBS analysis performed on mortars immersed in varying concentrations of chloride and sulfate. The findings revealed that at the wavelengths specific to Chloride(837.59nm) and Sulfur(921.30nm), the LIBS intensity achieved using AEM surpassed that obtained with a paper substrate at equivalent penetration concentrations. A robust correlation was confirmed between LIBS intensity and chloride ion concentration. Furthermore, when juxtaposed with IC analysis concentration outcomes at identical depths, the AEM displayed a higher intensity. The research noted an enhancement in LIBS intensity and a diminution in errors within the low-concentration section when deploying AEM. However, for the Sulfur wavelength of 921.3nm, there remains a need to augment the sensitivity of the LIBS signal within the low-concentration section in future studies. The findings underscore the potential of employing AEM and LIBS for precise analysis of chloride and sulfate ion penetration into mortar sections. This strategy can aid in bolstering assessment precision and mitigating errors, particularly in regions with low concentrations. It is recommended to further research and develop methods to amplify the sensitivity of the LIBS signal for sulfur detection in low-concentration sections. In sum, the study accentuates the significance of employing advanced techniques like AEM and LIBS for efficacious and precise analysis in the domain of mortar section assessment.

Effect of Deposition and Heat Treatment Conditions on the Electrical and Optical Properties of AZO/Cu/AZO Thin Film (증착 및 열처리 조건에 따른 AZO/Cu/AZO 박막의 전기적·광학적 특성 평가)

  • Chan-Young Kim;Ha-Eun Lim;Gaeun Yang;Sukjeang Kwon;Chan-Hee Kang;Sang-Chul Lim;Taek Yeong Lee
    • Korean Journal of Materials Research
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    • v.33 no.4
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    • pp.142-150
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    • 2023
  • AZO/Cu/AZO thin films were deposited on glass by RF magnetron sputtering. The specimens showed the preferred orientation of (0002) AZO and (111) Cu. The Cu crystal sizes increased from about 3.7 nm to about 8.5 nm with increasing Cu thickness, and from about 6.3 nm to about 9.5 nm with increasing heat treatment temperatures. The sizes of AZO crystals were almost independent of the Cu thickness, and increased slightly with heat treatment temperature. The residual stress of AZO after heat treatment also increased compressively from -4.6 GPa to -5.6 GPa with increasing heat treatment temperature. The increase in crystal size resulted from grain growth, and the increase in stress resulted from the decrease in defects that accompanied grain growth, and the thermal stress during cooling from heat treatment temperature to room temperature. From the PL spectra, the decrease in defects during heat treatment resulted in the increased intensity. The electrical resistivities of the 4 nm Cu film were 5.9×10-4 Ω·cm and about 1.0×10-4 Ω·cm for thicker Cu films. The resistivity decreased as the temperature of heat treatment increased. As the Cu thickness increased, an increase in carrier concentration resulted, as the fraction of AZO/Cu/AZO metal film increased. And the increase in carrier concentration with increasing heat treatment temperature might result from the diffusion of Cu ions into AZO. Transmittance decreased with increasing Cu thicknesses, and reached a maximum near the 500 nm wavelength after being heat treated at 200 ℃.

Effects of Brush Coating of Ag Nanowire Solution and Annealing using Plasma Process for Flexible Electronic Devices (유연 전자소자용 금속 전극 제조를 위한 Ag Nanowire 용액의 Brush 코팅 및 플라즈마 공정을 이용한 어닐링)

  • Kyoung-Bo Kim
    • Journal of Industrial Convergence
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    • v.21 no.3
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    • pp.189-194
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    • 2023
  • Recently, various studies on flexible electronic devices have been performed. In this study, the potential of Ag nanowires was evaluated as a material to replace the ITO transparent conductive film. Ag nanomaterials were formed on the glass by a novel brush coating method and an argon plasma evaporation method based on atmospheric pressure plasma. First, the Ag solution is coated on the glass with a brush, and the remaining solvent is removed with atmospheric plasma. During this process of solvent evaporation, a sound is generated by the reaction between the atmospheric plasma and the solvent. Therefore, the remaining amount of the solvent can be confirmed. In order to observe optical properties and electrical results such as reflectance, transmittance, and absorbance according to the number of coatings of the film, the results were analyzed by coating up to 5 times. For the purpose of investigating the interaction of light with Ag nanowires, reflectance and transmittance were measured while changing the wavelength of light from 200 nm to 800 nm. In the case of absorbance, the trend of increasing light absorption of the Ag nanowires according to the coating was clearly confirmed. The electrical properties showed a great change from the time of coating more than 4 times, and in particular, the resistance value was lower than kΩ/cm2 when the coating was applied 5 times. Based on these optical and electrical results, we plan to verify the possibility of a transparent conductive film by applying it to electronic devices in the future.

Comparison of the 2D/3D Acoustic Full-waveform Inversions of 3D Ocean-bottom Seismic Data (3차원 해저면 탄성파 탐사 자료에 대한 2차원/3차원 음향 전파형역산 비교)

  • Hee-Chan, Noh;Sea-Eun, Park;Hyeong-Geun, Ji;Seok-Han, Kim;Xiangyue, Li;Ju-Won, Oh
    • Geophysics and Geophysical Exploration
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    • v.25 no.4
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    • pp.203-213
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    • 2022
  • To understand an underlying geological structure via seismic imaging, the velocity information of the subsurface medium is crucial. Although the full-waveform inversion (FWI) method is considered useful for estimating subsurface velocity models, 3D FWI needs a lot-of computing power and time. Herein, we compare the calculation efficiency and accuracy of frequency-domain 2D and 3D acoustic FWIs. Thereafter, we demonstrate that the artifacts from 2D approximation can be partially suppressed via frequency-domain 2D FWI by employing diffraction angle filtering (DAF). By applying DAF, which employs only big reflection angle components, the impact of noise and out-of-plane reflections can be reduced. Additionally, it is anticipated that the DAF can create long-wavelength velocity structures for 3D FWI and migration.

Current Status and Future Prospects of Korean VLBI Network (KVN)

  • Jung, Taehyun;Sohn, Bong Won;So, Byunghwa;Oh, Chungsik;Je, Do-Heung;Byun, Do-Young;Jung, Dong-Kyu;Roh, Duk Gyoo;Lee, Euikyum;Kim, Hyo Ryoung;Kim, Hyun-Goo;Byun, Hyungkyu;Chung, Hyunsoo;Yim, In Sung;Kim, Jae-Young;Kim, Jaeheon;Yeom, Jaehwan;Shin, Jaesik;Park, Jeong-Je;Kim, Jeong-Sook;Hwang, Jungwook;Wajima, Kiyoaki;Song, Min-Gyu;Chung, Moon-Hee;Sakai, Nobuyuki;Lee, Sang-Hyun;Lee, Sang-Sung;Oh, Sej-Jin;Wi, Seog Oh;Kim, Seungrae;Kim, Soon-Wook;Lee, Sung-Mo;Kang, Yong-Woo;Minh, Young Chol;Kim, Young-Sik;Yun, Youngjoo
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.1
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    • pp.60.3-61
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    • 2021
  • The Korean VLBI Network (KVN) consists of three 21m radio telescopes installed in Seoul, Ulsan, and Jeju Island with the world's first 4-channel receiver that can observe four different frequencies (e.g., 22, 43, 86, 129 GHz) simultaneously. This receiving system of KVN is particularly effective in millimeter-wavelength VLBI (mm-VLBI) observations by compensating fast atmospheric fluctuations effectively. This technology is now being enhanced with a compact triple-band receiver, becoming the world standard for a mm-VLBI system. In 2020, KVN supported 54 observing programs (KVN: 28, EAVN: 26) including the 2nd KVN Key Science Program (KSP) which supports 8Gbps data recording rate and the East Asian VLBI Network (EAVN) programs. KVN also participated in the European VLBI Network (EVN) and GMVA (Global Millimeter VLBI Array) sessions regularly. Here, we report current status and future propsects of KVN.

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Oil Spill Monitoring in Norilsk, Russia Using Google Earth Engine and Sentinel-2 Data (Google Earth Engine과 Sentinel-2 위성자료를 이용한 러시아 노릴스크 지역의 기름 유출 모니터링)

  • Minju Kim;Chang-Uk Hyun
    • Korean Journal of Remote Sensing
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    • v.39 no.3
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    • pp.311-323
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    • 2023
  • Oil spill accidents can cause various environmental issues, so it is important to quickly assess the extent and changes in the area and location of the spilled oil. In the case of oil spill detection using satellite imagery, it is possible to detect a wide range of oil spill areas by utilizing the information collected from various sensors equipped on the satellite. Previous studies have analyzed the reflectance of oil at specific wavelengths and have developed an oil spill index using bands within the specific wavelength ranges. When analyzing multiple images before and after an oil spill for monitoring purposes, a significant amount of time and computing resources are consumed due to the large volume of data. By utilizing Google Earth Engine, which allows for the analysis of large volumes of satellite imagery through a web browser, it is possible to efficiently detect oil spills. In this study, we evaluated the applicability of four types of oil spill indices in the area of various land cover using Sentinel-2 MultiSpectral Instrument data and the cloud-based Google Earth Engine platform. We assessed the separability of oil spill areas by comparing the index values for different land covers. The results of this study demonstrated the efficient utilization of Google Earth Engine in oil spill detection research and indicated that the use of oil spill index B ((B3+B4)/B2) and oil spill index C (R: B3/B2, G: (B3+B4)/B2, B: (B6+B7)/B5) can contribute to effective oil spill monitoring in other regions with complex land covers.

Determination of residual novobiocin in livestock products and fisheries products by HPLC (HPLC를 이용한 축·수산 식품 중 잔류 노보비오신의 분석)

  • Lee, Byung Kyu;Lee, Cheol-Woo;Lee, Sang-Ju;Jung, Eun Ha;Lim, Hyun Kyun;Han, Sang Beom
    • Analytical Science and Technology
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    • v.20 no.4
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    • pp.347-354
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    • 2007
  • A simple and rapid high-performance liquid chromatography assay for the determination of residual novobiocin levels in bovine, porcine, chicken, flatfish and japanese eel muscle has been developed and validated. The separation condition for HPLC/UV was optimized with phenyl hexyl ($4.6{\times}150mm$, $5{\mu}m$) column with 10 mM monobasic sodium phosphate buffer (pH 2.5)/acetonitrile (50/50, v/v) as the mobile phase at a flow rate of 1.0 mL/min and detection wavelength was set at 254 nm. Residues were extracted from tissue by blending with methanol and lipid materials were removed with n-hexane. Then, the methanol extract was evaporated to dryness under a nitrogen stream, reconstituted in the mobile phase. Aliquot of the organic extract was decanted and filtered through $0.45{\mu}m$ syringe filter. The $20{\mu}L$ of the resulting solution was injected into the HPLC system. The calibration ranges were $0.5{\sim}5{\mu}g/g$ and calibration curves were linear with coefficients of correlation better than 0.95. The limits of quantification were $0.5{\mu}g/g$ for all muscles. The recoveries of bovine, porcine, chicken, flatfish and japaneseel muscles were 99.8%, 102.4%, 91.0%, 104.0% and 93.0%, respectively. The procedures were validated according to the CODEX guideline, determining specificity, linearity, accuracy, precision, quantitation limit and recovery.