• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.163-163
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.165-165
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.04a
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• pp.168-168
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• 2019

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.5-5
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• 2017

Too little and too much water due to climatic events is a significant cause of global food insecurity. Crops are less productive under water-limited conditions and all major crops, with the exception of rice (Oryza sativa), die within a few days of complete submergence. To complement our studies on genes such as SUB1A, (an ERF-VII transcription factor that provides robust submergence tolerance) and AG1 (a TREHALOSE 6-P PHOSPHATASE that promotes establishment of young seedlings underwater), we have retooled INTACT (${\underline{I}}solation$ of ${\underline{N}}uclei$ ${\underline{TA}}gged$ in specific ${\underline{C}}ell$ ${\underline{T}}ypes$) and TRAP (${\underline{T}}ranslating$ ${\underline{R}}ibosome$ ${\underline{A}}ffinity$ ${\underline{P}}urification$) for rice. These technologies enable us to follow dynamics in chromatin, nuclear pre-mRNAs and ribosome-bound mRNAs in meristems and diverse cell types. With these technologies we can better interpret responses to stresses and reestablishment of homeostasis. These include stress acclimation strategies involving changes in metabolism and development, such as dynamics in suberin deposition in sub-epidermal layers of roots that limit water loss under drought and oxygen escape during waterlogging. Our new data uncover dynamic and reversible regulation at multiple levels of gene regulation and provide new insights into processes of stress resilience. Supported by US NSF-PGRP Plasticity (IOS-1238243), Secretome (IOS-1546879) and REU (DBI-146129) grants.

• Korean Journal of Remote Sensing
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• v.40 no.3
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• pp.275-284
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• 2024

Monitoring crop growth changes and water content is crucial in the agricultural sector. This study utilized drones equipped with Short Wavelength Infrared (SWIR) sensors, sensitive to moisture changes, to observe soybeans' growth and water content variations. We confirmed that as soybeans grow more vigorously, their water content increases and differences in irrigation levels lead to decreases in vegetation and moisture indices. This suggests that waterlogging slows down soybean growth and reduces water content, highlighting the importance of detailed monitoring of vegetation and moisture indices at different growth stages to enhance crop productivity and minimize damage from waterlogging. Such monitoring could also preemptively detect and prevent the adverse effects of moisture changes, such as droughts, on crop growth. By demonstrating the potential for early diagnosis of moisture stress using drone-based SWIR sensors, this research suggests improvements in the efficiency of large-scale crop management and increases in yield, contributing to agricultural production.

• Journal of Life Science
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• v.27 no.10
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• pp.1111-1120
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• 2017

This study was conducted to select pepper lines that were tolerant to excessive water injury among the pepper germplasm and investigate the genetic characteristics of those lines to contribute to the breeding of pepper cultivars with stable productivity in abnormal weather. Each of the tolerant and susceptible lines went through immersion treatment, and differentially expressed genes between them were analyzed. The tolerant line showed increased expression of the CA02g26670 gene, which is involved in the CONSTANS protein pathway and regulation of flowering by day length, but it exhibited decreased expressions of CA01g21450, CA01g22480, CA01g34470, CA02g00370 and CA02g00380. The susceptible line showed increased gene expressions of CA02g09720, CA02g21290, CA03g16520, CA07g 02110, and CA12g17910, which are involved in the inhibition of proteolytic enzyme activity, DNA binding, inhibition of cell wall-degrading enzyme, and inhibition of nodulin, respectively. Meanwhile the expressions of CA02g02820, CA03g21390, CA06g17700 and CA07g18230 decreased in the susceptible line, in relation to calcium-ion binding, high temperature, synthesis of phosphocholine and cold stress, respectively. The expressions of genes related to apoptosis and peroxidase increased, while that of CA02g16990, which functions as a nucleoside transporter, decreased in both the tolerant and susceptible lines. Based on the different gene expressions between the tolerant and susceptible lines, further studies are needed on breeding abiotic stress-tolerant lines.

• Journal of agriculture & life science
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• v.51 no.1
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• pp.35-43
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• 2017

This research was carried out to excessive water tolerance test among corn accessions collected from India to breed corn cultivars targeting India market. The corn accessions were 20 Inbred lines and cultivars from India as well as Korean cultivars Gwangpyungok and Chaloksusu. Excessive water tolerance test was done in the green house by immerging the pots containing corn seedlings for two weeks. Then, the plant heights were measured to compare the control plants that were not grown in the immerging state. The results showed that seven accessions of high tolerance in flooding; H2(92.9%), H18(88.8%), CN114A(98.1%), CN351A(94.3%), Super900M(95.3%), P3394(98.8%), 31N27(96.7%) in which the percent is comparison to the control plants. Whereas nine accessions showed high damage by immerging; H1(78.9%), H8(73.4%), H10(77.1%), H19(79.0%), H26(74.1%), H31(75.7%), H34(77.5%), H36(77.4%), H40(74.6%). However, the reduction on the contents of chlorophyll a, chlorophyll b, carotenoids in leaf revealed contrast results to the flooding tolerance. Particularly, H36($7.249{\mu}g{\cdot}mg^{-1}$) and H40($7.642{\mu}g{\cdot}mg^{-1}$) showed rapid reduction in the chlorophyll a content during the flooding treatment. Whereas two Indian commercial varieties 37N27($0.630{\mu}g{\cdot}mg^{-1}$) and P3394($1.208{\mu}g{\cdot}mg^{-1}$) showed slight reduction. The reduction of chlorophyll a and carotenoid contents was positively correlated during the excessive water stress.