• 한국식품영양학회지
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• 제28권1호
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• pp.40-46
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• 2015

Hamcho (Salicornia herbacea) extracts were evaluated for total polyphenol content, antioxidant and pro-oxidant activities. The total polyphenol content was 1.81 g and 0.72 g per 100 g of dried sample in water and ethanol extracts respectively. Both water and ethanol extracts of Hamcho significantly exhibited antioxidant activity. The scavenging activity of hydroxyl radical was 13.8~26.2% and 14.2~16.0% in water and ethanol extracts respectively. The inhibitory effect of conjugated diene formation was 24.6~39.1% and 28.4~39.6% in water and ethanol extracts respectively. However, pro-oxidative effect was also observed in the Hamcho extracts. The Hamcho water extract showed the pro-oxidant effect by enhancing the formation of hydroxyl radical and conjugated diene. The ethanol extract of Hamcho induced conjugated diene formation at 0.5 mg/mL but not at 1 mg/mL. The hydroxyl radical formation was not induced by the Hamcho ethanol extract. Taken together, these results show that Hamcho extracts can act as pro-oxidants by generating hydroxyl radical or conjugated diene in addition to their antioxidant properties. Therefore, this study suggests that the physiological properties of Hamcho and its use as food materials should be considered with caution because antioxidant dietary plants such as Hamcho possess possible adverse effects induced by pro-oxidant activity.

• 한국산학기술학회논문지
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• 제14권10호
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• pp.5091-5096
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• 2013

본 연구는 여러 생리활성 성분이 다량 함유되어 있다고 알려진 전통 약용 식물인 어성초의 추출 조건에 따른 항산화력을 관찰하였는데, 추출조건으로는 식용으로 활용가능한 물 추출과 70% 에탄올 추출을 통해 항산화력을 비교하였다. 항산화력은 총 페놀 함량, DPPH 라디칼 소거능, ABS 양이온 소거능, FRAP 방법으로 측정하였다. 결과적으로 총 페놀 함량은 어성초 물추출물이나 70% 에탄올 추출물 모두 77.8 mM Tannic acid/g extract로 나타나 유의적인 차이는 없었고 DPPH 라디칼 소거능은 물 추출물 2 mg/mL 농도에서 78.4%, 70% 에탄올 추출물은 91.8%로 유의적으로 소거능이 70% 에탄올 추출 시에 우수한 것으로 나타났다. 또한 ABTS 양이온 소거능에서도 물 추출물은 2 mg/mL 농도에서 47.7%, 70% 에탄올 추출물에서는 54.8%로 유의적으로 70% 에탄올 추출물이 저해능이 우수한 것으로 나타났으며 FRAP 방법으로 통한 환원 능도 70% 에탄올 추출물이 우수한 것으로 관찰되었다. 이와 같은 결과로 어성초 추출물 조건중 70% 에탄올 추출 시 항산화력을 높힐 수 있는 생리활성 성분이 추출되는 것으로 사료되며 식용 가능한 기능성 식품소재로써 어성초 에탄올 추출물을 사용할 수 있을 것으로 사료된다.

• 한국식품저장유통학회지
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• 제7권2호
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• pp.195-200
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• 2000

Yields of various solvent extracts for Zanthoxylum schinifolium and Z. piperitum leaves were higher in ethanol extract layer. Ethanol Extract in sancho and chopi leaves was fractionated with different solvents, such as hexane, chloroform, ethyl acetate, butanol and water, yields of hexane fraction was higher. In the solvent extracts using methanol, ethanol, hexane, chloroform, and water, ethanol extract showed the most effective antimicrobial activities. Ethanol extract in sancho and chopi leaves was fractionated, the most antimicrobial activities of sancho leaf were ethyl acetate fraction, and chopi leaf were chloroform fraction.

• 생약학회지
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• 제23권1호
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• pp.24-28
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• 1992

Red ginseng extracts were prepared with various concentrations of ethanol. Extract yields were examined and saponins in the extracts were identified and determined by TLC and HPLC, respectively. Yields of the extracts, $19.7{\sim]50.3%$, were the highest in water extract and showed significant decrease with the increase of ethanol concentration used for extraction. Contrary to the extract yields, saponin yields from red ginseng were conspicuously increased with the increase of ethanol concentration and were $3.47{\sim}5.13%$ of crude saponins and $1.28{\sim}1.93%$ of six major ginsenosides. Saponin contents in the red ginseng extracts were $6.9{\sim}24.2%$, of crude saponin and $2.57{\sim}9.22%$, of six major ginsenosides.

• 동의생리병리학회지
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• 제24권5호
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• pp.854-858
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• 2010

Streptococcus mutans (S. mutans) plays an important role in the information of dental plaque and it is being noticed as major causative bacteria of dental caries. In the present study, inhibitory effects of the ethanol extract of Dianthus superbus Linne (D. superbus) on the growth, acid production, adhesion and water-insoluble glucan synthesis of S. mutans were examined. The ethanol extract of D. superbus (0.5 - 4 mg/ml) significantly lowered the growth of S. mutans in a dose dependent manner. The acid production of S. mutans were inhibited by the presence of ethanol extract of D. superbus(1 - 4 mg/ml) significantly. The ethanol extract of D. superbus (0.25 - 4 mg/ml) also significantly lowered the adherence of S. mutans in a dose dependent manner. In water-insoluble glucan synthesis assay, 0.25 - 4 mg/ml of the ethanol extract of D. superbus significantly inhibited the formation of water-insoluble glucan. These results suggest that D. superbus may inhibit the caries-inducing properties of S. mutans. Further studies are necessary to clarify the active constituents of D. superbus responsible for such biomolecular activities.

• 한국염색가공학회지
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• 제25권4호
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• pp.327-336
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• 2013

The purpose of this study was to evaluate the color image of the natural-dyed silk fabrics. The dye was extracted from pine needle by boiling pine needle with ethanol at $78^{\circ}C$ for 3hours and distilled water at $100^{\circ}C$ for 2hours. The 100% silk fabric was dyed of extract in pH 5 at $90-100^{\circ}C$ for 1 hr. As mordants used were compounds of Al, Sn, Fe, and Cr, color image of pine-needle dyed silk fabrics was classified into 5 factors (pure, gentle, sophisticate, comfortable, pastorale) and the factor pure is most important one of those. Most cheerful image in pure factor was from the fabrics dyed with ethanol extract and then, none and Cr mordanting. Dignified image was from the fabrics dyed with ethanol extract and then, Cu or Fe mordanting. In production, products dyed with ethanol extracts was preferred to those dyed with distilled water extracts. Color image and preference of the silk fabrics dyed with pine needles extracted was affected by extraction solvents and mordants.

• 한국염색가공학회지
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• 제28권3호
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• pp.195-207
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• 2016

In this study, we examined the influence of the pigment characteristic and dyeing condition on dyeing properties and functionality by using Perilla Frutescens L. Britt extracts, in which ethanol, distilled water and NaOH solution were used as 3 different solvents. Changes in dyeing conditions include variations in dye concentration, dyeing temperature, time and pH on dye uptake, and K/S values were compared according to these changes. Additionally, color changes were observed according to the use and types of mordant. Ultraviolet-visible spectrum was utilized to investigate the pigment characteristic, and as a result, chlorophyll was identified in ethanol extract, whereas tannin was identified both in distilled water extract and NaOH solution extract. By using FT-IR analysis, these tannins in distilled-water-extract and NaOH solution extract were verified to be hydrolyzable tannin. When dyeing silk, dye uptake increased as dye concentration, dyeing temperature and time increased, while it decreased as pH of the extract increased. Fabrics dyed without a mordant produced Y-series colors, and fabrics dyed with mordants showed various colors depending on the mordant types. Even though color fastness to washing and light was unsatisfactory, fastness to rubbing and perspiration showed relatively high grade. Moreover, deodorant ability of dyed fabric improved.

• 한국식품영양학회지
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• 제33권5호
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• pp.505-511
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• 2020

In this study, hot water and ethanol, acetone, and hexane extracts of the steamed Deodeok were investigated for use as raw materials for natural cosmetics. Hot water and ethanol, acetone and hexane extracts of steamed Deodeok did not indicate cell toxicity up to 300 ㎍/mL concentration in the MTT assay. The tyrosinase inhibition effects of the ethanol extract were higher than the ascorbic acid. The efficacy of the steamed Deodeok's ethanol extract on the elastase inhibition was similar to that of quercetin at higher concentrations. In conclusion, water, ethanol, acetone and hexane extracts of the steamed Deodeok indicated strong possibility as a whitening and anti-wrinkle functional cosmetic ingredient. Among them, the steamed Deodeok's ethanol extract, which showed excellent activity, is highly available because it has high safety compared to other organic solvent extraction methods used in cosmetics, and has minimal irritation to the skin.

• 한국식품과학회지
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• 제37권5호
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• pp.833-837
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• 2005

송화분, 참나무화분 및 백합화분에 대한 항산화 효능을 DPPH radical 소거능 및 동물조직의 지질산화 억제효능을 이용하여 평가하였다. 각 화분을 ethanol, 50% ethanol 및 물을 이용하여 추출물을 조제한 후 이들에 대한 DPPH radical 소거능을 분석한 결과 50% 소거능을 나타내는 $EC_{50}$ 값은, 송화분의 경우 50% ethanol 추출물(40.0mg/mL)이 가장 낮게 나타났으며 물 추출물(46.8mg/mL), 100% ethanol 추출물(131.2mg/mL) 순 이었다. 참나무화분에서는 50% ethanol 추출물(3.2mg/mL), 100%, ethanol 추출물(4.5mg/mL), 물 추출물(8.3mg/mL) 순이었고 백합화분에서는 100% ethanol 추출물의 $EC_{50}$값이 14.0mg/mL로, 50% ethanol 추출물(24.0mg/mL) 및 물 추출물(18.8mg/mL)에 비해 가장 낮았다. 3 종의 화분에서는 참나무 화분의 DPPH radical 소거능이 우수한 것으로 나타났다. 한편 $ascorbate-Fe^{3+}-EDTA$에 의해 유도되는 뇌조직에서의 지질산화도는 송화분, 참나무화분, 백합화분 추출물에 의해 모두 농도 의존적으로 억제되었으며 신장에서의 지질산화도 억제되었다. 그리고 이 중에서 송화분보다는 참나무와 백합화분의 효능이 우수한 것으로 나타났다. 화분 추출물에 대한 총 polyphenol의 함량을 분석한 결과 참나무화분$(32.5{\pm}2.9{\mu}g/mg\;pollen)$이 백합화분$(25.9{\pm}1.4{\mu}g/mg\;pollen)$이나 송화분$(9.3{\pm}0.7{\mu}g/mg\;pollen)$보다 높게 나타났다.

• 한국식품영양과학회지
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• 제37권10호
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• pp.1231-1237
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• 2008

포공영의 총 페놀함량에서는 열수 추출물 7.80$\pm$0.97 mg/g, 에탄올 추출물 9.12$\pm$0.51 mg/g으로 나타났다. 플라보노이드함량은 열수 추출물 54.20$\pm$1.95 mg/g, 에탄올 추출물 79.43$\pm$4.44 mg/g으로 항산화 활성에 관련된 페놀 및 플라보노이드 화합물들이 에탄올 추출물에 많이 존재함을 확인할 수 있었다. 전자공여능에 있어서도 포공영 에탄올 추출물이 열수 추출물 $IC_{50}$값에 비해 3배 정도의 뛰어난 활성을 보였다. 아질산염 소거능은 모든 pH 조건하에서 에탄올 추출물이 열수 추출물보다 높은 아질산염 소거능을 나타내었으며, pH가 증가할수록 감소하였다. SOD 유사활성을 측정한 결과 열수 추출물에서는 18.34$\sim$37.26 $\mu$g/mL, 에탄올 추출물에서는 16.60$\sim$49.03 $\mu$g/mL의 범위로 분석되었으며, 농도에 따른 유의적 증가현상을 나타내었다. Tyrosinase 및 elastase 저해활성은 모든 추출물에서 대조구에 비해 다소 약한 활성을 나타내었다. 포공영 추출물은 뛰어난 항산화 활성을 나타나 기능성 식품 및 화장품 소재로 개발 가능성이 높을 것으로 사료된다.