• 한국수산과학회지
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• 제43권6호
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• pp.606-613
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• 2010

In order to elucidate a mechanism responsible for the development of the odor characteristics of cooked, desirable-flavored shellfish, oysters were extracted using various solvents and the resulting extracts were evaluated organoleptically after cooking. The 80% aqueous methanol extract was found to produce a desirable cooked flavor. This oyster extract was fractionated using ion-exchange column chromatography and dialysis, and each of the fractions was subjected to cooking, followed by organoleptic evaluation. The outer dialysate fraction such as acidic and amphoteric water-soluble fractions produced a cooked oyster flavor. The volatile flavor compounds identified from cooked oyster included 29 hydrocarbons, 20 alcohols, 16 acids, 12 aldehydes, nine nitrogen-containing aromatic compounds, eight ketones, five furans, three esters, three phenols, and one benzene.

• 동의생리병리학회지
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• 제25권3호
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• pp.445-450
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• 2011

In order to validate the use of Olibanum as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effect of water-soluble extract of Olibanum (EO) on the expression of pro-inflammatory vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor-${\alpha}$. The extract inhibited dose-dependently VCAM-1 expression without its cytotoxic effect on HUVECs, as measured by a flow cytometer using fluorescence-enhanced anti-VCAM-1 antibody, and significantly decreased mRNA levels of VCAM-1, as determined using reverse transcription polymerase chain reaction. These results suggest that Olibanum may have therapeutic potential in the control of endothelial disorders caused by inflammation.

• 한국식품과학회지
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• 제25권5호
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• pp.526-529
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• 1993

전통기호음료성분에 의하여 나타나는 기능특성을 조사하기 위한 연구의 일환으로 결명자의 메탄올 추출물을 유기용매에 의한 분획, silica gel column chromatography, thin layer chromatography에 의하여 여러가지 획분으로 분획하여 아질산염 소거작용을 조사한 결과, 아질산염 소거율은 ethyl acetate 획분>chloroform 획분>수용성 획분>ethyl ether 획분의 순으로 나타났으며, ethyl acetate 획분에서 분리한 compound D의 아질산염 소거효과는 매우 강력하여 ascorbic acid보다 10배 뛰어났다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권3호
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• pp.236-238
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• 2004

This is the first report on the antitumor and immunostimulating activities of Ulva lactuca. Using the WSM (water-soluble fraction of a methanol extract from Ulva lactuca), a concentration of 140 g/mL was found to inhibit 50% of the human leukemia cell line U937 in growth, while splenocyte growth was stimulated up to a concentration of 100$\mu\textrm{g}$/mL. In addition, NO production by a macrophage cell line (RAW 264.7) and alkaline phosphatase activity in mouse splenocytes were both stimulated with 10$\mu\textrm{g}$/mL of WSM. Dose-dependent patterns were observed on all three cell-lines. Accordingly, the current results indicate that VUlva lactuca may be useful as a natural antitumor and immunostimulating agent.

• 대한의생명과학회지
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• 제18권1호
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• pp.10-15
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• 2012

A 12-week repeated-dose oral toxicity study of water-soluble Orostachys japonicus extract (WOJ) was performed in Sprague-Dawley (SD) rats of both genders. Each group of ten rats was orally administered in doses of either 0 or 250 mg/day over a 12-week period. As a result, no WOJ-related changes were observed in terms of survival rate, clinical signs, body weight, or food intake. In addition, no difference in organ weight between the control and treated groups was detected. Furthermore, serum biochemistry parameters revealed some changes within normal ranges although significant decreases in total-bilirubin in the females. In spite of some alterations in serum biochemistry, the clinical signs, body weight changes from food intake, and autoptical remarks indicated that WOJ was not toxic. This study suggests that repeated treatment of O. japonicus very low toxicity and the NOAEL (no observed adverse effect dose) of WOJ exceeds 250 mg/kg in the SD rats.

• Archives of Pharmacal Research
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• 제26권6호
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• pp.458-462
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• 2003

The antioxidant activity of the stem bark from Albizzia julibrissin was evaluated for its potential to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, to inhibit the generation of the hydroxyl radical ($\cdot OH$), total reactive oxygen species (ROS) and to scavenge authentic peroxynitrites ($ONOO^{-}$). The methanol extract of A. julibrissin exhibited strong antioxidant activity in the tested model systems. Therefore, it was further fractionated using several solvents. The antioxidant activity of the individual fractions were in the order of ethyl acetate (EtOAc) ＞ n-butanol (n-BuOH) ＞ dichloromethane ($CH_2 CI-2$) ＞ and water ($H_2O$). The ethyl acetate soluble fraction, which exhibited strong antioxidant activity, was further purified by repeated silicagel, Sephadex LH-20 and RP-18 gel column chromatography. Sulfuretin (1) and 3 ,4 ,7-trihydroxyflavone (2) were isolated as the active principles. Compounds 1 and 2 exhibited good activity in all tested model systems. Compound 1 exhibited five times more inhibitory activity on the total ROS than Trolox. Compound 2 showed six times stronger DPPH radical scavenging activity than L-ascorbic acid. These results show the possible antioxidant activity of the A. julibrissin crude extract and its major constituents.

• 한국작물학회지
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• 제45권5호
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• pp.310-315
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• 2000

Alfalfa (Medicago sativa L.) plants have been reported to contain water-soluble substances that are autotoxic as well as allelopathic. Laboratory experiment through a petri-dish assay with imbibed seeds was conducted to evaluate both autotoxic and allelopathic effects of alfalfa leaf extracts on the germination and early seedling growth of alfalfa, red clover, crested wheatgrass, and Russian wildrye. Alfalfa seed germination was delayed dependent on extract concentration, with no difference in final germination at 72 hours. Root growth of alfalfa was stimulated up to 14% above control at very low concentrations of both leaf and stem extracts of alfalfa and was significantly reduced at extract concentration of more than 0.5g dry tissue/L (${gL}_{-1}$). Leaf extracts were generally more autotoxic for root growth than were stem extracts. Hypocotyl growth was not affected by all the concentrations of both leaf and stem extracts. Root length of legumes was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Hypocotyl growth of two legume plants and plant height of two grasses were not influenced by extracts. Seed germination and root growth of legumes were more inhibited by aqueous extracts of alfalfa leaf than were those of grasses. This result indicates autotoxic effect of alfalfa leaf extracts seems to be greater than allelopathic effect.

• 한국축산식품학회지
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• 제38권6호
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• pp.1168-1178
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• 2018

The present study aimed to characterise anti-oxidant peptides from water-soluble protein extracts of Hanwoo beef and evaluate their anti-proliferative effect on human colorectal carcinoma cells (HCT116). Antioxidant peptides were purified from the low-molecular-weight fraction (<3 kDa) of Hanwoo beef extract. Antioxidant activity of peptide fractions was determined using the oxygen radical absorbance capacity (ORAC) assay. Purified peptide (P3) displayed higher ORAC activity than the low-molecular-weight fraction ($202.66{\mu}M\;TE/g$ vs $167.38{\mu}M\;TE/g$ of dry matter, respectively) (p<0.05). The peptide sequence of P3 was Cys-Cys-Cys-Cys-Ser-Val-Gln-Lys (888.30 Da). The novel peptide P3, at $250{\mu}g/mL$, also significantly inhibited HCT116 cell proliferation up to 25.24% through phosphorylation of ERK, JNK, and p38 kinase (p<0.05). Hence, antioxidant peptide P3 from Hanwoo beef extract can be used as an antioxidative and anticancer agent in the functional food industry.

• 한국식품저장유통학회지
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• 제12권2호
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• pp.146-150
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• 2005

오가피(Acanthopanax senticosus)로부터 영양 및 기능성 성분의 열수추출 수율을 증대시킬 수 있는 방법을 개발하기 위하여 추출 전처리 및 방법의 효과를 검토하였다. 오가피를 분쇄, 체질$(8\~20\;mesh)$하고 초음파(20 kHz), 마이크로파 (2,450 MHz) 또는 볶음$(180^{\circ}C)$ 처리를 10분 동안 실시한 후 물을 용매로 상압$(100^{\circ}C)$ 추출과 가압$(121^{\circ}C)$ 추출을 각각 2시간 동안 실시하였다. 전반적으로 전처리는 성분들의 추출수율과 유리기 소거능을 증가 시키는 경향을 보였다. 특히, 가용성 고형물, 총당 및 페놀성 물질의 추출수율은 마이크로파와 초음파에 의해, 단백질의 추출수율과 유리기 소거능은 볶음처리에 의해 더욱 증가됨을 보였다. 상압추출보다 가압추출에 의해 가용성 고형물, 총당, 단백질 및 페놀성 물질의 추출수율이 증대되는 경향을 보였다. 이로써 초음파, 마이크로파 및 볶음 전처리 및 가압추출은 오가피의 추출효율 향상에 유효한 방법인 것으로 판단된다.

• 한국식품과학회지
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• 제13권4호
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• pp.334-339
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• 1981

보리차제조(製造)에 있어서 볶음공정(工程)을 확립(確立)하고자 볶음온도(溫度)와 시간(時間)을 달리한 조건(條件)에서 얻은 보리차에 대하여 외관색도(外觀色度), 추출갈변색소(抽出褐變色素) 및 수용성(水溶性) 고형분량(固形分量)을 분석(分析)하고 이들의 상호관계(相互關係)를 검토(檢討)하였다. 외관색도(外觀色度)를 명도단위(明度單位) Y값으로 표현(表現)할 때 볶은 통보리의 Y값과 이를 분쇄(粉碎)한 분말(粉末)의 Y값간(間)의 상관관계(相關關係)는 $0.957{\sim}0.994%$에 달(達)하여 표면(表面)의 Y값의 측정(測定)으로 내부(內部)의 색(色)을 예측(豫測) 및 대변(代辯)할 수 있었다. 보리의 볶음정도(程度)는 볶음에 사용된 열량(熱量)의 합계 보다는 볶음 온도(溫度)에 의하여 크게 좌우(左右)되었다. 볶음보리의 수용성(水溶性) 성분(成分)의 함량(含量)은 $232^{\circ}C$에서 25분(分)간(間)의 볶음에서 최대(最大)값을 보였으며 그 함량(含量)은 68%이었다. 볶음통보리의 Y값과 볶음 온도(溫度), 갈변색소(褐變色素) 및 가용성(佳容性) 고형분(固形分) 함량(含量)과의 관계(關係)를 이용하여 볶음정도(程度)를 판정(判定)하고 볶음공정(工程)을 조절(調節)하는 기준(基準)으로 활용(活用)할 수 있었다.