• Title/Summary/Keyword: water plasma

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Fractionation of Plasma Protein on the Several Fresh Water Fishes by Disc Electrophoresis (Disc 전기영동법에 의한 수종 담수어 혈장 단백질의 분획)

  • 홍사욱;박성배
    • YAKHAK HOEJI
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    • v.22 no.1
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    • pp.42-50
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    • 1978
  • The plasma proteins of fresh water fishes have been fractionated by disc electrophoresis in acrylamide gels utilized as an electrophoretic supporting medium. The species of fishes examined in this experiment were Anguilla japonica, Misgurnus mizolepis, Parasilurus asotus, Siniperca scherzeri, Pelteobagrus fulvidra, Carassius carassius, Cyprinus carpio, and Hemibarbus labeo, obtained in the Han River. Disc electrophoresis was performed as described by Ornstein and Davis. Gels and buffer solution were prepared by the method developed by W.J.Kim. The separation gels were 7% acrylamide gel. The fractionation of plasma proteins showed 13 bands in Anguilla japonica, 10 in Misgurnus mizolepis, 15 in Parasilurus asotus, 12 in Siniperca scherzeri, 11 in Pelteobagrus fulvidra, 13 in Carassius carassius, 9 in Cyprinus carpio, and 13 in Hemibarbus labeo. The patterns of plasma protein on the each species of fishes were different in the number of bands, ratio of contents, relative mobilities, and forms of fractionation.

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Effect of HF and Plasma Treated Glass Surface on Vapor Phase-Polymerized Poly(3,4-ethylenedioxythiophene) Thin Film : Part I

  • Lee, Joonwoo;Kim, Sungsoo
    • Journal of Integrative Natural Science
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    • v.6 no.4
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    • pp.211-214
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    • 2013
  • In this study, in order to investigate how consecutive treatments of glass surface with HF acid and water vapor/Ar plasma affect the quality of 3-aminopropyltriethoxysilane self-assembled monolayer (APS-SAM), poly(3,4-ethylenedioxythiophene) (PEDOT) thin films were vapor phase-polymerized immediately after spin coating of FeCl3 and poly-urethane diol-mixed oxidant solution on the monolayer surfaces prepared at various treatment conditions. For the film characterization, various poweful tools were used, e.g., FE-SEM, an optical microscope, four point probe, and a contact angle analyzer. The characterization revealed that HF treatment is not desirable for the synthesis of a high quality PEDOT thin film via vapor phase polymerization method. Rather, sole treatment with plasma noticeably improved the quality of APS-SAM on glass surface. As a result, a highly dense and smooth PEDOT thin film was grown on uniform oxidant film-coated APS monolayer surface.

Plasma Etching and Polymerization of Carbon Fiber (플라즈마 에칭과 중합에 의한 탄소섬유의 표면 개질)

  • H. M. Kang;Kim, N. I.;T. H. Yoon
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2002.05a
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    • pp.143-146
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    • 2002
  • Unsized AS-4 carbon fibers were etched by RF plasma and then coated via plasma polymerization in order to enhance adhesion to vinyl ester resin. The gases utilized for the plasma etching were Ar, $N_2 and O_2$, while the monomers used for the plasma polymerization coating were acetylene, butadiene and acrylonitrile. The conditions for the plasma etching and the plasma polymerization were optimized by measuring interfacial adhesion with vinyl ester resin via micro-droplet tests. Among the treatment conditions, the combination of Ar plasma etching and acetylene plasma polymerization provided greatly improved interfacial shear strength (IFSS) of 69MPa compared to 43MPa with as-received carbon fiber. Based on the SEM analysis of failure surface and load-displacement curve, it was assume that the failure might be occurred at the carbon fiber and plasma polymer coating. The plasma etched and plasma polymer coated carbon fibers were subjected to analysis with SEM, XPS, FT-IR or Alpha-Step, and dynamic contact angles and tensile strengths were also evaluated. Plasma polymer coatings did not change tensile strength and surface roughness of fibers, but decreased water contact angle except butadiene plasma polymer coating, possibly owing to the functional groups introduced, as evidenced by FT-IR and XPS.

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Effects of Se Pye San Water Extract on the Plasma Cortisol Concentration, Arterial Blood $PCO_2$ and $PO_2$ in the Rabbit (세폐산(洗肺散) 전탕액(煎湯液)이 가토(家兎).혈장(血漿) Cortisol 농도(濃度) 및 동맥혈(動脈血) $PCO_2,\;PO_2$에 미치는 영향(影響))

  • Kim, Dae-Won;Han, Sang-Hwan
    • The Journal of Internal Korean Medicine
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    • v.11 no.1
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    • pp.41-52
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    • 1990
  • After intravenous administration of Se Pye San water extract in the rabbit, the change of plasma cortisol concentration, arterial blood $PCO_2$ and $PO_2$ was obtained such results as follows. 1. The plasma cortisol concentration in the control group was constant, but after intravenous administration of Se Pye San water extract at the dose of 0.2 ml/kg, the above concentration was increased significantly from 2 to 3 hours. Also, the above concentration was increased remarkably at the dose of 0.4 ml/kg from 1 to 4 hours. 2. After intravenous administration of Se Pye San water extract at each dose of 0.2 ml/kg and 0.4 ml/kg, arterial blood $PCO_2$ was decreased remarkably from 1 to 4 hours. 3. No change after intravenous administration of Se Pye San water extract at the dose of 0.2 ml/kg, while arterial blood $PO_2$ was decreased significantly at the dose of 0.4 ml/kg on 3 hours. As a results of the above, the therapeutic action of Se Pye San water extract effected with Jisu (止嗽), Jeong Cheon (定喘), Geo Dam, Cheong Yeol (淸熱) would be related with the increased both plasma cortisol concentration and arterial blood $PO_2$, and the decrease of arterial blood $PCO_2$.

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Curing of meat batter by indirect treatment of atmospheric pressure cold plasma

  • Jo, Kyung;Lee, Juri;Lim, Yubong;Hwang, Jaejun;Jung, Samooel
    • Korean Journal of Agricultural Science
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    • v.45 no.1
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    • pp.94-104
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    • 2018
  • Nitrite is an essential additive for cured meat product. Plasma is ionized gas and reactive nitrogen species in plasma can be infused into meat batter and subsequently generate nitrites by reaction with water molecules after plasma treatment. However, the increase of nitrite in meat batter is limited with direct treatment of atmospheric pressure cold plasma because of the increase of meat batter temperature. Therefore, this study investigated the influence of indirect treatment of atmospheric pressure cold plasma on the physicochemical properties of meat batter. Meat batter was indirectly treated with plasma at 1.5 kW for 60 min. The pH of meat batter decreased while the temperature increased with plasma treatment time. The total aerobic bacterial count of meat batter was not affected by plasma treatment. The nitrite content of meat batter was increased to 377.68 mg/kg after 60 min of plasma treatment. The residual nitrite content of cooked meat batter also increased with plasma treatment time. The CIE $a^*$-value of cooked meat batter increased. As plasma treatment time increased, lipid oxidation tended to increase and protein oxidation significantly increased. According to these results, the indirect treatment of atmospheric pressure cold plasma can be used as a new curing method for replacing synthetic nitrite salts.

Development of Plasma Reactor of Dielectric Barrier Discharge for Water Treatment (수처리용 유전체 장벽 방전 플라즈마 반응기 개발)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.21 no.5
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    • pp.597-603
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    • 2012
  • Non-thermal plasma processing using a dielectric barrier discharge (DBD) has been investigated as an alternative method for the degradation of non-biodegradable organic compounds in wastewater. The active species such as OH radical, produced by the electrical discharge may play an important role in degrading organic compound in water. The degradation of N, N-Dimethyl-4-nitrosoaniline (RNO) was investigated as an indicator of the generation of OH radical. The DBD plasma reactor of this study consisted of a plasma reactor, recycling pump, power supply and reservoir. The effect of diameter of external reactor (15 ~ 40 mm), width of ground electrode (2.5 ~ 30 cm), shape (pipe, spring) and material (copper, stainless steel and titanium) of ground electrode, water circulation rate (3.1 ~ 54.8 cm/s), air flow rate (0.5 ~ 3.0 L/min) and ratio of packing material (0 ~ 100 %) were evaluated. The experimental results showed that shape and materials of ground were not influenced the RNO degradation. Optimum diameter of external reactor, water circulation rate and air flow rate for RNO degradation were 30 mm, 25.4 cm/s and 4 L/min, respectively. Ground electrode length to get the maximum RNO degradation was 30 cm, which was same as reactor length. Filling up of glass beads decreased the RNO degradation. Among the experimented parameters, air flow rate was most important parameters which are influenced the decomposition of RNO.

Disinfection of Escherichia coli and Bacillus subtilis using underwater plasma

  • Yu, Seung-Min;No, Tae-Hyeop;Seok, Dong-Chan;Yu, Seung-Ryeol;Hong, Yong-Cheol;Lee, Bong-Ju
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.47-47
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    • 2010
  • Discharge under the water is very hard and demand considerable high voltage. But specially improved electrode can generate plasma discharge to salty water with relatively low voltage. A round shape ceramic electrode having many pinholes combined with metallic one can generate plasma. 400 volt, 10 kHz and 3 micro second pulse width were applied to repeatedly running synthetic seawater with 10 L/m velocity, containing cultivated E. coli and Bacillus. As a result, 18, 94, 99.97, 100, 100 % disinfection rates to E. coli and 17.1, 17.1, 82.9, 99.4, 99.9 % disinfection rates to Bacillus subtilis were achieved to 1, 2, 3, 4, 5 times repetitive treatment respectively. In the plasma condition, the ions and electrons are separated and new kinds of components are re-synthesized by the intensive movement of the components. Especially chlorine ions are separated and recombined to residual free chlorine like HOCl, $OCl^-$. The residual free chlorine concentrations of discharged water were 0.25, 0.88, 1.39, 1.59, 1.66 mg $Cl_2$/L after 5 times treatment respectively. Another unconfirmed radical and oxidants for example, OH, $H_2O_2$, and $O_3$ can have an effect on microorganism of course.

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Toxic effects of arsenic on growth, hematological parameters, and plasma components of starry flounder, Platichthys stellatus, at two water temperature conditions

  • Han, Jae-Min;Park, Hee-Ju;Kim, Jun-Hwan;Jeong, Dal-Sang;Kang, Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.22 no.1
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    • pp.3.1-3.8
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    • 2019
  • The purpose of this study is to investigate the changes in growth, hematological parameters, and plasma components of juvenile starry flounder, Platichthys stellatus, following exposure to varying arsenic concentrations present at different water temperatures. P. stellatus (total length, $15.9{\pm}0.4cm$; body weight, $62.2{\pm}4.2g$) were exposed for 4 weeks to waterborne arsenic (sodium arsenite, As) at 0, 150, 300, and $600{\mu}g/L$ at temperatures of $12^{\circ}C$ and $18^{\circ}C$. Toxic effects of As exposure on P. stellatus were higher at the higher temperature and the growth and hematological parameters measured decreased with increasing arsenic concentration, while the concentration of plasma components measured increased. This indicates that waterborne As exposure and water temperature can cause toxic effects on growth, hematological parameters, and plasma components in Platichthys stellatus.

Effects of Saseuptang Water Extracts on the Renal Function in Rats (사습탕(瀉濕湯) 전탕액(煎湯液)이 백서(白鼠)의 신장기능(腎臟機能)에 미치는 영향(影響))

  • Min, Young-Kie;Yu, Yun-Cho;Lee, Ho-Sub;Ryu, Do-Gon
    • The Journal of Korean Medicine
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    • v.17 no.1 s.31
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    • pp.212-221
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    • 1996
  • The aim of this experiment was to elucidate the effects of Saseuptang water extract on the renal function plasma renin activity and plasma levels of atrial natriuretic peptide and aldosterone in rat The results were as follows; 1. Water balance decreased significantly after the administration of Saseuptang water extract, 0.4 and 0.8ml/kg. 2. Urine volume increased significantly after the administration of Saseuptang water extract, $0.4\;m{\ell}/kg$. 3. Urinary excretion of chloride increased significantly after the administration of Saseupthang water extract, $0.8\;m{\ell}/kg$. 4. Free water clearance increased significantly after the administration of Saseuptang water extract, $0.8\;m{\ell}/kg$. 5. Urinary excretion of creatinine increased significantly after the administration of Saseuptang water extract, $0.8\;m{\ell}/kg$. 6. Plasma levels of atrial natriuretic peptide (ANP) decreased significantly after administration of Saseupthang water extract, $0.8\;m{\ell}/kg$. These results suggest that the changes of urine volume after the administration of Saseuptang water extracts are related to the increments of glomerular filtration rate and free water clearance, and it is suggested that the changes of renal function by which Saseuotang may related to the renin-angiotensin and atrial natriuretic peptide system.

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The Study on Characteristics of N-Doped Ethylcyclohexane Plasma-Polymer Thin Films

  • Seo, Hyeon-Jin;Jo, Sang-Jin;Lee, Jin-U;Jeon, So-Hyeon;Bu, Jin-Hyo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.540-540
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    • 2013
  • In this studying, we investigated the basic properties of N-doped plasma polymer. The N-doped ethylcyclohexane plasma polymer thin films were deposited by radio frequency (13.56 MHz) plasma-enhanced chemical vapor deposition method. Ethylcyclohexenewas used as organic precursor (carbon source) with hydrogen gas as the precursor bubbler gas. Additionally, ammonia gas [NH3] was used as nitrogen dopant. The as-grown polymerized thin films were analyzed using ellipsometry, Fourier-transform infrared [FT-IR] spectroscopy, Raman spectroscopy, FE-SEM, and water contact angle measurement. The ellipsometry results showed the refractive index change of the N-doped ethylcyclohexene plasma polymer film. The FT-IR spectrashowed that the N-doped ethylcyclohexene plasma polymer films were completely fragmented and polymerized from ethylcyclohexane.

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