• Food Science and Biotechnology
• /
• 제17권2호
• /
• pp.389-395
• /
• 2008

The objective of this study was to examine the effects of crude protease from Bacillus polyfermenticus SCD and marination time on quality of pork and beef jerky. Neither pork nor beef jerky showed a significant difference in pH among all treatments, and each protease was found to have a greater effect on the color of beef jerky. The hardness was significantly lower in all jerky treated with each protease, however the textural properties of jerky were not significantly different with regard to marination times. Water content was not affected by protease addition or marination times, however the water activity was lower in jerky treated with protease. The rehydration capacity of pork jerky was higher in jerky treated with protease, whereas that of beef jerky was higher in jerky dried after tumbled and held for 24 hr. Sensory characteristics were higher in jerky treated with protease, not affected by holding time after marinated.

• Food Science and Biotechnology
• /
• 제17권1호
• /
• pp.203-207
• /
• 2008

The present study aimed at assessing the protective effect of water extract from fruit body of the Grifola frondosa (GFW) on carbon tetrachloride ($CCl_4$)-induced hepatotoxicity. Rats orally administered with GFW 0.5, 1.0, 2.0 g/kg for 14 days were treated with $CCl_4$ to induce hepatotoxicity. Pretreatment with GFW remarkably prevented the elevation of serum AST, ALT, ALP, LDH, $\gamma$-GTP, and liver lipid peroxides in $CCl_4$-treated rat and GFW administration in liver injured rats by $CCl_4$ showed significant (p<0.05) protection of liver as evidenced from normal serum enzymes and malondialdehyde (MDA) levels. In the ultrastructural changes, administration of $CCl_4$-induced damage of hepatocytes with vacuolation, a highly damaged endoplasmic reticulum, and degenerating nuclei. However, pre-administration with GFW preserved normal ultrastructure of hepatocytes. These results suggest that GFW had an effect to inhibit $CCl_4$-induced liver injury in rat, and that it could be used as an effective hepatoprotective agent against chemical-induced liver damage.

• Preventive Nutrition and Food Science
• /
• 제17권4호
• /
• pp.293-298
• /
• 2012

In this study, we designed a method to manufacture elevated fermented green tea by using Monascus pilosus, which is known as a functional microbe, and observe its antioxidant abilities and quality characteristics. The water-soluble substance (WSS) content of the fermented tea by M. pilosus (FTM) was lower than that of the non-fermented tea (NFT), although the alcohol-insoluble substance (AIS) content of the FTM was higher than that of NTM. On the other hand, the fractionated distilled water-soluble polysaccharide (DWSP), CDTA-soluble polysaccharides (CDSP), sodium carbonate-soluble polysaccharide (SCSP) and KOH soluble hemicellulose (HC) obtained from the AIS of the FTM was markedly higher than that of NFT. In the antioxidant parameters, the electron donating ability of all fractions, except HC, extracted from FTM was higher than that of NFT, and iron chelating ability of all fractions, except CDSP, extracted from FTM was higher than that of NFT. Whereas the DWSP and SCSP obtained from the FTM were higher than that of NFT, the activity of the HC fraction from both NFT and the FTM could not be detected. In addition, the xanthin oxidase (XO) inhibitory activities of the DWSP, CDSP and the SCSP obtained from the NFT were significantly higher than that of FTM, the aldehyde oxidase (AO) inhibitory activities of the DWSP and SCSP extracted from the FTM were markedly higher than that of the NFT. Meanwhile, the acceptance of NFT and FTM had no significant difference, while the quality of aroma, taste and mouthfeel of the FTM was higher than that of NFT. These results suggest that the post-fermented tea by Monascus microorganisms may be responsible for functional components as well as contribute to the improvement of the tea quality.

• 한국해양바이오학회지
• /
• 제2권3호
• /
• pp.149-154
• /
• 2007

상어연골에서 발생하는 갈변과 냄새를 제거하기 위한 오존수 세척효과와 crude chondroitin sulfate의 추출특성을 조사하기 위하여 알칼리 및 열수추출 효과를 조사하였다. SC 분말을 용존 오존농도 0.3 ppm의 오존수로 세척한 결과 일반성분의 함량에는 영향을 미치지 않으면서 $L^*$값은 증가하고 $a^*$, $b^*$값은 감소되어 탈색효과가 뚜렷하였다. 또한 ammonia 유사취가 크게 감소되었으며 종합적 기호도가 크게 향상되었다. NaOH에 의한 CS의 최적 추출농도는 1 N이었으며 최적 추출시간은 $100^{\circ}C$에서 120분이었고 이때의 수율은 10.02%이었다. $100^{\circ}C$의 열수로 7회 반복추출 할 경우 1 N NaOH 추출수율과 대등하였다.

• Journal of Applied Biological Chemistry
• /
• 제64권1호
• /
• pp.5-11
• /
• 2021

말오줌나무 잎 추출물의 생리활성 및 항균활성을 측정한 결과, 말오줌나무 잎 추출물의 생리활성은 말오줌나무 잎 추출물에 함유되어있는 phenolic성분들에 의해 효과가 발현되는 것으로 판단되었다. 말오줌나무 잎으로부터 생리활성에 관여하는 phenolic 화합물을 물과 에탄올로 추출하였을 때 각각 11.60±0.18과 12.39±0.07 mg/g의 함량을 나타내었다. 말오줌나무 잎의 ACE 저해 활성은 50-200 ㎍/mL의 phenolic을 함유한 물과 에탄올 추출물에서 각각 71.94-92.08와 48.42-78.33%의 저해 활성이 측정되었으며, 농도의존적인 양상을 나타내었다. 말오줌나무 잎 추출물의 XOase에 대한 추출물의 저해 활성은 50-200 ㎍/mL의 phenolic을 함유한 물과 에탄올 추출물에서 각각 42.11-100와 35.61-100%의 매우 우수한 저해효과를 나타내었다. 말오줌나무 잎 추출물에 대한 HAase 저해활성은 200 ㎍/mL의 phenolic을 함유한 농도에서 물과 에탄올 추출물이 각각 25.35±0.41와 43.38±2.26%의 HAase 저해활성을 나타내었다. 말오줌나무 잎 추출물의 항균활성은 충치균인 S. mutans와 식중독균인 S. aureus 및 여드름균인 P. acnes에 대한 우수한 항균 효과를 나타내었다. 따라서 말오줌나무는 고혈압억제, 통풍억제 및 염증억제 작용 등의 생리활성 및 S. mutans와 S. aureus 및 P. acnes에 대한 항균활성이 우수한 것으로 확인되어 말오줌나무를 활용한 기능성 식품 소재로서 산업화에 활용할 수 있을 것으로 생각되었다.

• 한국식품저장유통학회지
• /
• 제1권1호
• /
• pp.1-7
• /
• 1994

To investigate the effect of several bactericides and fungicides against fruit & vegetable decay grapefruit seed extract(GFSE) was tested in a dilution solution. GFSE was shown to be effective against decay and rind breakdown and to extend the shelf-life of fruits and vegetables during storage and tiansport. Antimicrobial efficiency of GFSE on a wide spectra of gram + & - bacteria, moulds and yeasts was demonstrated by the measurement of minimal inhibitory concentrations performed (Bacteria 10-500ppm, Fungi:250-1,000ppm, Yeasts:100-250ppm). GFSE was separated and extracted into water-soluble fraction, water-insoluble and non-dialyzed fraction to isolate the antimicrobial substances. The water-soluble fraction showed the most active antimicrobial effect. The antimicrobial substances were isolated by gas chromatography. As the result of the isolation using GC, Peak-D was found to be the antimicrobial compound in GFSE. The identification of the most antimicrobial substance was carried out by using GC-MS.

• 한국식품영양학회지
• /
• 제33권6호
• /
• pp.732-736
• /
• 2020

Theobroma cacao L., a fruit of cacao trees, is a perennial plant, which belongs to Sterculiaceae, and is native to the Amazon in South Africa. It also has been known for its various biologically active effects, such as anti-oxidation, anti-cancer, and anti-bacterial. The spleen cell proliferations of mice were measured at 48 hours after treatment of Theobroma cacao L. water extracts in seven concentrations(0, 5, 10, 50, 100, 250, 500 and 1,000 ㎍/mL) an ELISA assay. The production of cytokine (IL-1β, TNF-α, IFN-γ), is secreted by macrophages stimulated with LPS, was detected by ELISA assay using the cytokine kit. From the results of in vitro study, both splenocytes and cytokine production activated by peritoneal macrophages have increased when water extracts were supplemented in the range between 250 and 500 ㎍/mL concentration. Notably, splenocytes production has a signigicant proliferation at 500 ㎍/mL concentration. The result from this research suggests that supplementation with Theobroma cacao L. water extracts may enhance the immune function by stimulating the splenocyte proliferation and improving the cytokine production activating macrophage in vitro.

• 한국식품영양학회지
• /
• 제34권3호
• /
• pp.289-294
• /
• 2021

The antioxidant activity and α-glucosidase inhibitory activity of the solvent fraction fractionated from the methanol extract of Saururus chinensis Baill were examined. As a result of measuring the yields of methanol, hexane, chloroform, ethylacetate, butanol, and water fractions, the extraction yield of fraction was 18.60, 3.38, 24.03, 7.75, 8.11 and 62.57%, respectively. The total polyphenol content of the methanol extract of Saururus chinensis Baill was 13.40, 4.62, 7.39, 31.24, 25.76 and 5.64 mg GAE/g, respectively. DPPH radical scavenging activity (IC_50%) results were 20.81, 5.47, 10.15, 22.63, 19.68 and 21.06 ug/mL, respectively, and hydroxyl radical scavenging activity (IC_50%) results were 15.81, 2.69, 8.84, 12.80, 3.70 and 3.39 ug/mL. Hydrogen peroxide scavenging activity scavenging activity measurement (IC_50%) showed 33.63, 8.88, 16.93, 32.84, 33.79, and 33.71 ug/mL in methanol, hexane, chloroform, ethyl acetate butanol, and water fractions, respectively. The α-glucosidase inhibitory activity of the solvent fraction fractionated with the methanol extract of 300 sec was measured for the α-glucosidase inhibitory activity of methanol, hexane, chloroform, ethyl acetate butanol, and water fraction, respectively, 15.85, 10.84, 15.74, 24.90, 2.58 and 35.70%.

• 한국식품영양과학회지
• /
• 제39권10호
• /
• pp.1495-1502
• /
• 2010

단감의 이용성을 증대하기 위하여 전과와 과육을 각각 동결건조한 후 이화학적 특성 및 생리활성을 비교하였다. 섬유소, 비타민 C 및 무기물 함량은 전과에서 높았으나, 아미노산의 함량은 과육에서 약 1.4배 정도 높았다. 동결건조 된 단감의 물 및 에탄올 추출물 중 총 페놀화합물은 전과에서 유의적으로 높았으나, 플라보노이드 함량은 유의차가 없었다. 추출물의 농도를 달리하여 생리활성을 비교한 결과, 물추출물보다는 에탄올 추출물, 과육에 비해 전과에서 활성이 높았다. 특히, ABTS, NO 라디칼 소거능, 환원력 및 tyrosinase 저해활성은 모든 농도에서 전과가 과육에 비해 유의적으로 높았다. 따라서 단감 가공 시 전과의 이용은 섬유소 및 비타민 C의 섭취량뿐만 아니라 생리활성의 증가에도 유효할 것으로 예상되었다.

• 한국식품영양과학회지
• /
• 제46권3호
• /
• pp.394-399
• /
• 2017

플라스틱 포장 폐기물로 인한 환경오염을 해결하기 위한 대안으로 생분해성 필름이 많이 연구되어 왔다. 본 연구에서는 우럭 껍질 젤라틴(RFG)과 nano-clay(Cloisite $Na^+$, 10A)의 복합필름의 특성에 관하여 연구하였다. 우럭 껍질로부터 젤라틴을 추출하여 sorbitol을 가소제로서 첨가한 단백질 필름을 제조하였고, 제조된 RFG 필름의 tensile strength(TS), water vapor permeability(WVP), water solubility(WS)는 각각 15.0 MPa, $2.70{\times}10^{-9}g\;m/m^2\;s\;Pa$, 53.8%였다. RFG에 nano-clay를 첨가한 복합필름은 TS가 증가하고 WVP와 WS는 감소하였다. XRD 분석과 SEM 측정 결과를 통하여 RFG/nano-clay 복합필름 내부에 박리형 구조를 형성함을 확인할 수 있었다. 결론적으로 본 연구 결과 RFG/nano-clay 복합필름은 생분해성 포장재로 적용될 수 있음을 시사한다.