• Restorative Dentistry and Endodontics
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• 제19권1호
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• pp.169-179
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• 1994

The purpose of this study was to compare and evaluate the bleaching efficacy of three nonvital bleaching techniques: Walking bleaching, Walking bleaching and heat application, and Walking bleaching and bleaching light application. 36 extracted anterior teeth with intact crowns were immersed in 5 % sodium hypochlorite solution for twenty-four hours to loosen extrinsic debris. Lingual access openings were prepared in all teeth and the pulps were extirpated. The teeth were stored in 5% sodium hypochlorite for twenty-four hours to open the dentinal tubules and they were stained via whole blood. Once the teeth were stained, they were evaluated with Colorimeter. The teeth in each group were ranked from lightest to darkest and divided equally into three experimental groups in which the following bleaching techniques were used. Intracoronal base was placed 2mm below cementoenamel junction. Group 1 Walking bleaching (Superoxol + Sodium perborate) Group 2 Heat application + Walking bleaching (Superoxol+Sodium perborate) Group 3 Light application+Walking bleaching (Superoxol+Sodium perborate) The bleaching agents were changed every 3 days and the teeth were bleached for a total of 14 days. The teeth were evaluated with Colorimeter before the start of any bleaching and on day 14. The results were as follows: 1. At the end of 14 days, all the sample teeth demonstrated the increase of Lightness Index at cervical 1/3 of crown (p<0.05) Lightness Index was significant difference in group 2 and 3, but there were some minor differences among groups (p>0.05). 2. In all groups, there was significant difference in red chromacity (p<0.05), but there were some minor differences among groups (p>0.05). 3. In all groups, there was no significant difference in yellow chromacity (p>0.05).

• Restorative Dentistry and Endodontics
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• 제23권2호
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• pp.656-669
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• 1998

Hydrogen peroxide at high concentration during walking bleaching may cause damage to the tooth structure and to the surrounding periodontal tissues and may develop external root resorption. Clinically, It is so important to find a method of prevention or minimization of these complications. The efficacy of various chamber-irrigating agents to eliminate residual hydrogen peroxide after walking bleaching was examined and compared with water rinse in this study. Extracted human 46 premolars without any cementoenamel junction defects were treated endodontically and based with IRM to 1 mm below CEJ and totally bleached 3 times for each tooth with 30% hydrogen peroxide and sodium perborate. Upon completion of the 3rd walking bleaching procedure, the cervical portion and pulp chamber of each group of teeth were irrigated with catalase, 70% ethylalcohol, acetone, and distilled water. And then, a radicular hydrogen peroxide penetration was measured with spectrophotometer immediately after each bleaching and following treatment with each chamber-irrigating agents, and the significance of their eliminating efficacy of residual hydrogen peroxide was analyzed by Kruskal-Wallis test. The results were obtained as follows. 1. Cervical root penetration of hydrogen peroxide was increased as the bleaching procedure was repeated(P<.01). 2. The most effective irrigant that removed residual hydrogen peroxide was the catalase, and the least effective one was water rinsing (P<.01).; there was no significant difference between the acetone and ethanol group. 3. The Irrigation with antioxidant enzyme or water-displacement solutions can eliminate residual oxygen radicals from the pulp chamber effectively after walking bleaching. So, these agents can reduce adverse effects such as cervical external resorption and periapical inflammation and prevent residual $O_2$ from impeding composite resin polymerization, thus increase the bonding strength of composite resin. This, in turn reduces microleakage and discoloration of the esthetic restoration, extending its service-life.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.19-34
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• 1996

It has been demonstrated that intracoronal bleaching of pulpless teeth may result in cervical root resorption. Several authors postulated that bleaching agents such as hydrogen peroxide penetrated through the dentinal tubules to damage the surrounding tissues that cause cervical root resorption. The purpose of this study was to suggest on in vitro model for direct determination of hydrogen peroxide penetration through CEJ during nonvital bleaching. In addition, this model permit the quantification of the amount of hydrogen peroxide penetrated during the procedure. Freshly extracted intact premolars, removed for orthodontic reasons were used. Root canal treatment was performed in each tooth. And then the outer surface and crown portion of the teeth was sealed with wax leaving the CEJ. The prepared teeth mounted on the wax laminates were placed in plastic assay tubes containing 1.5ml bidistilled water with their entire root, including the CEJ, submerged in the solution. The teeth were dividied into four groups. Thermo group : thermocatalytic bleaching with superoxol Walk group: walking bleaching with sodium perborate & superoxol Combi group : combination of thermocatalytic & walking bleaching Dw group : walking bleaching with sodium perborate & water The bleaching procedure was performed three times. The bleaching intervals were at 3 days. The hydrogen peroxide present in the assay system was added to ferrous ammonium sulfate resulting in ferric ion release. Upon the addition of potassium thiocyanate a ferrithiocyanate complex results, which absorbs light at the wavelength of 467nm. The radicular penetration of hydrogen peroxide in the four groups was assessed directly using spectrophotometer. The amount of hydrogen peroxide in the samples tested is determined by comparing them with a standard curve generated by known amounts of hydrogen peroxide. The results were obtained as follows : 1. In all experimental groups except the Dw group showed lower penetration amount in day 4 than day 1, there was statistical importance in the difference (P<0.05). 2. After 3rd treatment, Thermo group showed slightly increased value and narrow distribution. Walk group showed much more penetration amount and widely dispersed value. Value of Combi group showed wide distribution without regard to treatment time, but value of Dw group evenly distributed. 3. Thermo group, Walk group and Dw group showed a tendency of increasing penetration amount with increasing treatment times(P<0.01), but Combi group revealed no statistically important differences. 4. Combi group showed the highest degree of penetration. Walk group showed lower penetration than Combi group. Thermo group & Dw group showed lower than Walk group. 5. Cervical root permeability to hydrogen peroxide varied from 0 to 35 %.

• 대한심미치과학회지
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• 제4권1호
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• pp.12-22
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• 1995

The purposes of this study were to compare the bleaching efficacy of sodium perborate when mixed either with Superoxol or distilled water and to evaluate the efficacy of different location of intracoronal base on the presence of apical leakage of tested bleaching agents. Forty eight extracted human permanent incisors were stained via whole blood and canal fillings with conventional gutta percha were performed after routine biomechanical perparations. The experimental intracoronal base was placed either at the cementoenamel junction(group 3, 4) or 2mm below cementoenamel junction(group 1, 2). Walking bleaching was performed by two different combinations of bleaching agents : sodium perborate with distilled water in group 1, 3 and sodium perbrate with Superoxol in group 2, 4. The roots of the teeth were evaluated for the presence of color change to assess the leakage of bleaching agents and the cervical one-thirds of the crown were evaluated for bleaching effect from the whiteness Indea calculated by spectrophotometer. The results were as follows : 1. At the end of 12 days, all the sample teeth demonstrated the increase of Whiteness Index at cervical 1/3 of crown although there were some minor differences among groups. 2. Regardless of location of the base, sodium perborate with superoxol(group 2, 4) showed better results in bleaching than the sodium perborate with distilled water(group 1, 3). 3. Bleaching agent leaked into the root area when the base was placed 2mm below cementoenamel junction but no leakage was found when the base was placed at the cementoenamel junction.

• Restorative Dentistry and Endodontics
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• 제18권1호
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• pp.145-155
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• 1993

The purposes of this study were to compare the bleaching efficacy of sodium perborate when mixed either with Superoxol or distilled water and to evaluate the efficacy of different location of intracoronal base on the presence of apical leakage of tested bleaching agents. Forty eight extracted human permanent incisors were stained via whole blood and canal fillings with conventional gutta percha were performed after routine biomechanical perparations. The experimental intracoronal base was placed either at the cementoenamel junction(group 3, 4) or 2mm below cementoenamel junction(group 1, 2). Walking bleaching was performed by two different combinations of bleaching agents : sodium perborate with distilled water in group 1, 3 and sodium perbrate with Superoxol in group 2, 4. The roots of the teeth were evaluated for the presence of color change to assess the leakage of bleaching agnets and the cervical one-thirds of the crown were evaluated for bleaching effect from the whiteness Indea calculated by spectrophotometer. The results were as follows : 1. At the end of 12 days, all the sample teeth demonstrated the increase of Whiteness Index at cervical 1/3 of crown although there were some minor differences among groups. 2. Regardless of location of the base, sodium perborate with superoxol)group 2, 4) showed better results in bleaching than the sodium perborate with distilled water(group 1, 3). 3. Bleaching agent leaked into the root area when the base was placed 2mm below cementoenamel junction but no leakage was found when the base was placed at the cementoenamel junction.

• Restorative Dentistry and Endodontics
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• 제28권1호
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• pp.57-63
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• 2003

The purpose of the present study were to evaluate microleakage of a fourth generation dentin-bonding agent following a walking bleaching treatment, to determine the effect of temporary postbleaching dressing with calcium hydroxide on microleakage and to investigate the effect of delayed intracoronal restoration on microleakage. The results of this study were as follows : 1. Bleached groups showed more microleakage than unbleached group 2. Immediately restored group following bleaching procedure showed the highest microleakage score. 3. One-week delayed restorations showed less microleakage but there were no statistically significant difference between group II and III. 4. Provisional dressing with calcium hydroxide had no influence on microleakage. It is necessary to know the time that has elapsed from the bleaching treatment to the restoration procedure to achieve optimal seal, as well as to reduce the risk of microleakage in adhesive restoration.

• Restorative Dentistry and Endodontics
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• 제27권4호
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• pp.441-447
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• 2002

The bleaching of discolored nonvital teeth is conservative treatment that satisfy the cosmetic desire. The most common method for this treatment, walking bleaching, is using 30% hydrogen peroxide and sodium perborate. Many alternatives are suggested for preventing the external cervical root resorption that is the common complication of the nonvital teeth bleaching with 30% hydrogen peroxide The same extent of oxidation reactions as that resulted by the bleaching with the application of 30% hydrogen peroxide and sodium perborate can also be acquired more safely by materials that contain 10% carbamide peroxide, used primarily for the bleaching of vital teeth. Therefore, this study was performed to evaluate the efficacy of 10% and 15% carbamide peroxide bleaching gel in nonvatal teeth bleaching. The internal bleaching of intentionally discolored teeth was performed in vitro with 10% carbamide peroxide (Group 1), 15% carbamide peroxide (Group 2), mixture of distilled water and sodium perborate (Group 3), and mixture of 30% hydrogen peroxide and sodium perborate (Group 4). The bleaching materials were refreshed following 3, 6, 9 and 12 days. To evaluate the bleaching effect, the color change of the crowns was measured at 1, 2, 3, 4, 7 and 15 days of bleaching using the colorimeter. The results were as follows：1. L$^*$ and $\Delta$E$^*$ values were increased with time in all bleaching agents (p<0.01). 2. There was no significant difference in L$^*$ and $\Delta$E$^*$ value among bleaching agents. 3. $\Delta$E$^*$ value higher than 3 was shown after 3 days of bleaching with 10% carbamide peroxide gel, 1 day with 15% carbamide poroxide gel, 4 days with mixture sodium perborate and distilled water and 4 days with mixture sodium perborate and 30% hydrogen peroride, respectively. These results revealed that the use of 10% and 15% carbamide peroxide bleaching gel in non-vital teeth bleaching is as effective as mixture of distilled water and sodium perborate and mixture of 30% hydrogen peroxide and sodium perborate. Accordingly, carbamide peroxide could be used clinically to bleach discolored non-vital teeth.

• 대한소아치과학회지
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• 제21권2호
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• pp.593-598
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• 1994

There were two cases of esthetic improvement of discolored teeth. First case was 12-year-old girl whose upper right central incisor was discolored after traumatic injury. She was treated by walking bleaching technique. Endodontic retreatment was done, and then the mixture of Superoxol and sodium perborate was applied and changed every week. After 3 weeks, esthetic result was obtained and fracture site was rcovered with light-curing composite resin. Second cases was 11-year-old girl whose teeth were discolored by fluorosis. She was treated by vital bleaching technique. A resin splint was made and then 30% and 15% carbamide peroxide was applied using this splint. After 4 weeks, a noticeable improvement in the color and the brightness of the teeth was achieved.

• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2001년도 추계학술대회(제116회) 및 13회 Workshop 제3회 한ㆍ일 치과보존학회 공동학술대회 초록집
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• pp.584.2-584
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• 2001

The purpose of the present study were to evaluate microleakage of a fourth generation dentin-bonding agent following a walking bleaching treatment, to determine the effect of temporary postbleaching dressing with calcium hydroxide on microleakage and to investigate the effect of delayed intracoronal restoration on micro leakage. Forty extracted non-carious incisors were selected and conventional root canal treatment was performed. The teeth were randomly divided into 4 groups of 10 specimens each.(omitted)

• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1636-1643
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• 2012

Enzymatic pre-bleaching by modification of pulp fibers with xylanases is an attractive approach to reduce the consumption of toxic bleaching chemicals in the paper industry. In this study, an alkaliphilic endoxylanase gene was isolated from metagenomic DNA of a structurally stable thermophilic lignocellulose-degrading microbial consortium using amplification with conserved glycosyl hydrolase family 10 primers and subsequent genome walking. The full-length xylanase showed 78% sequence identity to an endo-${\beta}$-1,4-xylanase of Clostridium phytofermentans and was expressed in a mature form with an N-terminal His6 tag fusion in Escherichia coli. The recombinant xylanase Xyn3F was thermotolerant and alkaliphilic, working optimally at $65-70^{\circ}C$ with an optimal pH at 9-10 and retaining >80% activity at pH 9, $60^{\circ}C$ for 1 h. Xyn3F showed a $V_{max}$ of 2,327 IU/mg and $K_m$ of 3.5 mg/ml on birchwood xylan. Pre-bleaching of industrial eucalyptus pulp with no prior pH adjustment (pH 9) using Xyn3F at 50 IU/g dried pulp led to 4.5-5.1% increase in final pulp brightness and 90.4-102.4% increase in whiteness after a single-step hypochlorite bleaching over the untreated pulp, which allowed at least 20% decrease in hypochlorite consumption to achieve the same final bleaching indices. The alkaliphilic xylanase is promising for application in an environmentally friendly bleaching step of kraft and soda pulps with no requirement for pH adjustment, leading to improved economic feasibility of the process.