• Title/Summary/Keyword: volumetric productivity

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Continuous Production of Succinic Acid Using an External Membrane Cell Recycle System

  • Kim, Moon-Il;Kim, Nag-Jong;Shang, Longan;Chang, Yong-Keun;Lee, Sang-Yup;Chang, Ho-Nam
    • Journal of Microbiology and Biotechnology
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    • v.19 no.11
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    • pp.1369-1373
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    • 2009
  • Succinic acid was produced by continuous fermentation of Actinobacillus succinogenes sp. 130Z in an external membrane cell recycle reactor to improve viable cell concentration and productivity. Using this system, cell concentration increased to 16.4 g/l at the dilution rate $0.2\;h^{-1}$, up to 3 times higher than that of batch culture, and the volumetric productivity of succinic acid increased up to 6.63 g/l/h at the dilution rate $0.5\;h^{-1}$, 5 times higher than that of batch fermentation. However, in the continuous culture using a high dilution rate, operational problems including severe membrane fouling and contamination by lactic acid producer were observed. Another succinic acid producer, Mannheimia succiniciproducens MBEL55E, was also utilized in this system, and the cell concentration and productivity of succinic acid at the dilution rate of $0.3\;h^{-1}$ were found to be above 3 and 2.3 times higher, respectively, compared with those obtained at the dilution rate of $0.1\;h^{-1}$. These observations give a deep insight into the process design for a continuous succinic acid production by microorganisms.

Study of PSA Process for Carbon Dioxide Recovery over Zeolite Adsorbent: Effect of Rinse Rate on Process Performance (제올라이트 흡착제 상에서 이산화탄소 회수를 위한 PSA 공정 연구: 공정성능에 대한 세정유량의 영향)

  • 전종기;박영권;주국택
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.1
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    • pp.99-110
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    • 2004
  • In order to investigate the performance of carbon dioxide PSA over zeolite adsorbent, the present study showed results of isotherm measurement, cyclic operation of 3-bed apparatus, and the corresponding numerical simulation. The experiment data of bed temperature, purity, recovery were matched well with that of numerical simulation. Purity of both gas and adsorbent phase increased rapidly with rinse rate but the degree of increase was retarded for large rinse rate The total amount of adsorbed increased only 10% even if rinse rate was enlarged to 4 times. Optimal rinse rate was 7N㎥/hr in this study. The heating rinse led to augments in recovery and productivity, possibly thanks to ease of description resulting from increased volumetric rinse rate and temperature rise in the column.

Production of Shikonin by A Hairy Root Culture of Lithospermum erythrorhizon

  • Seo, Weon-Taek;Park, Young-Hoon;Choe, Tae-Boo
    • Journal of Microbiology and Biotechnology
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    • v.2 no.1
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    • pp.41-45
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    • 1992
  • Shikonin production was examined in a bubble column bioreactor system with the hairy roots of Lithosphermum erythrorhizon. The volumetric productivity was higher than those obtained from other reactor configurations with free or immobilized cells of the same cell line. The productivities of the bubble column reactor, with and without a product absorption trap, were 7.4 and 4.5 mg of shikonin/l/d, respectively. This indicated the importance of the product removal in the design and operation of the shikonin production system with hairy root culture.

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Citric Acid Production by Succharomycopsis lipolytica in Air-lift and Membrane Recycle Bioreactors (기포탑 및 막 재순환 생물반응기에서의 Saccharomycopsis lipolytica에 의한 구연산 생산)

  • 조대철;정봉현;장호남
    • Microbiology and Biotechnology Letters
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    • v.17 no.6
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    • pp.624-628
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    • 1989
  • A study on the citric acid production using Saccharomycopsis lipolytica (NRRL Y7576) was carried out in shake-flasks, air-lift and membrane recycle bioreactors. The cells entrapped in Ca-alginate beads were used in shake-flasks and air-lift reactor. Repeated batch fermentation in shake-flasks was successfully performed for 34 days and resulted in a yield of 54%. Increased yield (63%) was obtained in the air-lift reactor operation using nitrogen deficient medium (NDM). In the membrane recycle bioreactor operation, the maximal dry cell mass concentration was 39 g/1 at a dilution rate of 0.02 h$^{-1}$ and the yield with NDM was higher than that with growth medium. In addition, the yield and volumetric productivity with pure oxygen supply were greatly improved compared with those with air supply.

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Compensation of Thermal Error for the CNC Machine Tools (I) - The Basic Experiment of Compensation Device - (CNC 공작기계의 열변형 오차 보정 (I) - 보정장치 기초실험 -)

  • 이재종;최대봉;곽성조;박현구
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 2001.04a
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    • pp.453-457
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    • 2001
  • One of the major limitations of productivity and quality in metal cutting is the machining accuracy of machine tools. The machining accuracy is affected by geometric and thermal errors of the machine tools. In this study, the compensation device is manufactured in order to compensate thermal error of machine tools under the real-time. This paper models of the thermal errors for error analysis and develops on-the-machine measurement system by which the volumetric error are measured and compensated. The thermal error is modeled by means of angularity errors of a column and thermal drift error of the spindle unit which are measured by the touch probe unit with a star type styluses, a designed spherical ball artifact, and five gap sensors. In order to compensate thermal characteristics under several operating conditions, experiments performed with five gap sensors and manufactured compensation device on the horizontal machining center.

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Production of fumaric acid with biofilm contactor by Rhizopus oryzae KCTC 6946

  • Kim, Jin-Nam;Wi, Yeong-Jung;Yun, Jong-Seon;Ryu, Hwa-Won
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.243-246
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    • 2002
  • In this study, biological production of fumaric acid by Rhizopus oryzae KCTC 6946 using rotary biofilm contactor was investigated. In study of neutralizing agent on fumaric acid production, $Na_2CO_3$ was more effective than NaOH. After 24 hr of incubation with a rotating speed of 10 rpm at $35^{\circ}C$, biofilm was grown on and around the surface of the disks. The yield and volumetric productivity of rotary biofilm contactor were 33.8% and 0.595 g/L${\cdot}$h, respectively, with the optimum effective disk surface area of 1,583 $cm^2/L$.

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Experimental Investigation of Laser Spot Welding of Ni and Au-Sn-Ni Alloy

  • Lee, Dongkyoung
    • Journal of Welding and Joining
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    • v.35 no.2
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    • pp.1-5
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    • 2017
  • Many microelectronic devices are miniaturizing the capacitance density and the size of the capacitor. Along with this miniaturization of electronic circuits, tantalum (Ta) capacitors have been on the market due to its large demands worldwide and advantages such as high volumetric efficiency, low temperature coefficient of capacitance, high stability and reliability. During a tantalum capacitor manufacturing process, arc welding has been used to weld base frame and sub frame. This arc welding may have limitations since the downsizing of the weldment depends on the size of welding electrode and the contact time may prevent from improving productivity. Therefore, to solve these problems, this study applies laser spot welding to weld nickel (Ni) and Au-Sn-Ni alloy using CW IR fiber laser with lap joint geometry. All laser parameters are fixed and the only control variable is laser irradiance time. Four different shapes, such as no melting upper workpiece, asymmetric spherical-shaped weldment, symmetric weldment, and, excessive weldment, are observed. This shape may be due to different temperature distribution and flow pattern during the laser spot cutting.

Increased Poly(3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli from Whey by Agitation Speed Control

  • Kim, Beom-Soo;Brian K. O'Neill;Lee, Sang-Yup
    • Journal of Microbiology and Biotechnology
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    • v.10 no.5
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    • pp.628-631
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    • 2000
  • The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

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Development of Certified Reference Materials for Specific Surface Area (비표면적 인증표준물질 개발)

  • Choi, Byung Il;Kim, Jong Chul;Kim, Taeyoung;Nham, Hyunsoo;Kwon, Su Yong
    • Analytical Science and Technology
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    • v.18 no.1
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    • pp.74-84
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    • 2005
  • Understandings of adsorption characteristic of interface are very important in several advanced materials processes, related to NT and BT technology. Volumetric gas adsorption method, suitable for absolute measurements, is regarded as the standardized measurement technique for specific surface area. In order to verify the reliability of commercial equipments, certified reference materials (called CRM) of specific surface area are developed and evaluated its uncertainty factors by standard equipment which has traceability to SI units. Specific surface areas of developed materials are $10.72{\pm}0.46m^2g^{-1}$ for silicon nitride powders and $149.50{\pm}3.44m^2g^{-1}$ for alumina. These disseminations of CRMs would result in improved reliability chains in industrial processes, and lead eventually to contribution to productivity improvement, quality management, safety evaluation, and possibly to new material development.

Geomechanical and thermal reservoir simulation during steam flooding

  • Taghizadeh, Roohollah;Goshtasbi, Kamran;Manshad, Abbas Khaksar;Ahangari, Kaveh
    • Structural Engineering and Mechanics
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    • v.66 no.4
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    • pp.505-513
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    • 2018
  • Steam flooding is widely used in heavy oil reservoir with coupling effects among the formation temperature change, fluid flow and solid deformation. The effective stress, porosity and permeability in this process can be affected by the multi-physical coupling of thermal, hydraulic and mechanical processes (THM), resulting in a complex interaction of geomechanical effects and multiphase flow in the porous media. Quantification of the state of deformation and stress in the reservoir is therefore essential for the correct prediction of reservoir efficiency and productivity. This paper presents a coupled fluid flow, thermal and geomechanical model employing a program (MATLAB interface code), which was developed to couple conventional reservoir (ECLIPSE) and geomechanical (ABAQUS) simulators for coupled THM processes in multiphase reservoir modeling. In each simulation cycle, time dependent reservoir pressure and temperature fields obtained from three dimensional compositional reservoir models were transferred into finite element reservoir geomechanical models in ABAQUS as multi-phase flow in deforming reservoirs cannot be performed within ABAQUS and new porosity and permeability are obtained using volumetric strains for the next analysis step. Finally, the proposed approach is illustrated on a complex coupled problem related to steam flooding in an oil reservoir. The reservoir coupled study showed that permeability and porosity increase during the injection scenario and increasing rate around injection wells exceed those of other similar comparable cases. Also, during injection, the uplift occurred very fast just above the injection wells resulting in plastic deformation.