• 생물환경조절학회지
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• 제20권3호
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• pp.197-203
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• 2011

고구마 재배농가에서 무병묘를 손쉽게 자가증식할 수 있는 간편한 수경재배 시스템을 도입하기 위하여, 점적호스를 이용한 순환식 자루재배 시스템을 시설하여 배지 혼합비율 및 삽수종류에 따른 무병묘의 생육을 조사하였다. 연황미와 맛나미 모두 15일경까지는 배지 종류에 따른 생육에 유의한 차이를 보이지 않았으나, 20일경부터는 원예용상토(TKS-2)와 펄라이트 함량이 높은 혼합배지(코코피트 : 펄라이트, 3 : 7 v/v)에서 생육이 양호하였다. 품종 간에는 연황미의 생육이 맛나미보다 양호하였으며, 1마디 삽수보다 정단 삽수로 증식하는 것이 효과적이었다. 연황미와 맛나미의 줄기생육은 정단 삽식에서 1마디 삽식보다 10cm 및 2cm 이상 신장하였으며, 코코피트와 펄라이트 3 : 7(v/v) 혼합배지에서의 건물중과 생체중은 원예용 상토 또는 코코피트와 펄라이트 7 : 3(v/v) 혼합배지에서보다 유의한 증가를 보였다. 따라서 코코피트와 펄라이트(3 : 7, v/v) 혼합배지를 이용한 자루재배는 고구마 무병묘 증식에 성공적으로 적용할 수 있었으며, 고구마 종순농가에서도 큰 경제적 부담없이 적용할 수 있을 것으로 기대되었다.

• 생물환경조절학회지
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• 제29권1호
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• pp.1-8
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• 2020

고구마 바이러스 무병묘의 기내급속증식을 위한 적정 생장 조절물질 및 sucrose 농도, 최소생장 기내보존(15℃)에 미치는 광의 영향과 생존율 및 기내생장 특성 등을 조사하였다. 고구마 무병묘의 마디배양은 0.2 mg·L^-1 BA 첨가배지에서 줄기신장, 줄기직경, 잎수, 뿌리수, 생체중 및 건물중 등이 가장 양호하였다. 배양부위 및 배지물리성에 따른 적정 sucrose 농도는 마디배양은 5% sucrose를 첨가한 고체배지에서, 정단배양은 3% sucrose를 첨가한 액체배지에서 줄기두께, 잎수, 뿌리수, 뿌리길이, 생체중 및 건물중 등의 생육에 가장 효과적이었다. 15℃ 저온항온기에서 고구마 무병묘의 최소생장 기내보존은 암상태에서는 3개월 내에 모두 고사하였으나, 적색:청색(7:3) 혼합 LED (150±5 μmol·m^-2·s^-1 PPFD)에서는 5개월까지 100% 생존하였다. 따라서 고구마 무병묘의 최소생장 기내보존에는 광이 필요하며, 샬레에 밀식(10 개체/샬레)할 경우, 좁은 공간에서 대량보존이 가능하였다.

• 원예과학기술지
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• 제30권6호
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• pp.686-693
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• 2012

NFT 수경재배 방식을 이용한 고구마 바이러스 무병주 대량 증식 기술을 개발하기 위하여, 버미큘라이트와 펄라이트(1:1, v/v)를 채운 72공 플러그 트레이(35mL/cell)에 삽목하여 양액 종류, 삽수 크기 및 품종에 따른 생육특성을 평가하였다. 무병묘의 줄기신장, 생체중 및 건물중 등은 일본원시배양액, 한국원시배양액, 야마자키 상추배양액의 순으로 양호한 생육을 보였다. 삽수 종류에 따른 무병묘의 생육은 줄기 정단 삽수에서 가장 양호하였으며, 삽수 크기가 클수록 줄기 길이, 생체중 및 건물중의 증가가 뚜렷하였다. 품종 간에는 '신자미'와 '연황미'의 줄기신장, 생체중 및 건물중 증가가 다른 품종에 비하여 양호하였다. 줄기 굵기와 뿌리 길이는 삽수 부위 및 크기와 품종 간에 유의차가 없었다. 1마디 삽수는 2마디 삽수와 생육특성에서 유의한 차이를 보이지 않았다. NFT 수경 재배에서 무병주 대량 증식 효율은 1마디 삽수와 정단 삽수를 이용하는 것이 효과적이었다.

• Journal of Microbiology
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• 제40권3호
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• pp.183-192
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• 2002

Cells infected With equine herpesvirus type-1 (EHV-1) Produced both infectious and non-infectious Virus-related particles. Compared to the whole virion, non-infectious particles termed L-particles were deter-mined to lack 150 kDa protein, commonly known as nucleocapsid protein. The potential of L-particles to induce immune responses was studied in mice and foals. Intranasal immunization with L-particles or whole virions induced poor IgG antibody responses in mice. Interestingly, despite the poor antibody response, the conferred immunity protected the host from challenge infections. This was indicated by a significant reduction in virus titers in line with recovery towards normal body weight. Subsequently, the test on the usefulness of L-particles as immunizing agents was extended to foals. Immunization of specific-pathogen-free (SPF) foals resulted in similar results. As determined by a complement-fixing-antibody test (CFT), foals seroconverted when they were immunized either with inactivated L-particles or whole virions via intramuscular (i.m.) injections. The presence of the antibody correlated with the degree of protection. Beyond day 1 post challenge infection (p.i.), there was no virus shedding in the nasal mucus of foals immunized with whole EHV-1 virions. Virus shedding was observed in foals Immunized with L-particles but limited to days 6 to 8 p.i. only. In contrast, extended vim shedding was observed in non-immunized foals and it was well beyond day 14 p.i. Viremia was not detected for more than four days except in non-immunized foals. Immunization in mice via intranasal (i.n.) conferred good protection. However, compared to the i.n. route, a greater degree of protection was obtained in foals following immunization via i.m. route. Despite variation in the degree of protection due to different routes of immunization in the two animal species, our results have established significant evidence that immunization with L-particles confers protection in the natural host. It is suggested that non-infectious L-particles should be used as immunizing agents for vaccination of horses against EHV-1 infection.

• Journal of Plant Biology
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• 제7권1호
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• pp.1-4
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• 1964

A comparative study of free amino acid content in healthy and virus diseased tobacco leaves was carried out by author throughout the gorwing season from June to November of 1963. The methods of qualitative analysis of free amino acids applied in this experiment is followed by Moore and Stein. 1,2 Free amino acids determined in this experiment are shown in Fig. Ⅰ, Ⅱ and Table Ⅰ. As the figure and the table are shown, four more amino acids such as a spartic acid, glutamic acid, tyrosine and phenylalanine are detected in the healthy leaves; these four additional amino acids in the healthy leaves are conspicuous. More quantities of asparagine and alanine are detected in the diseased leaves than the healthy leaves and more quantities of tryptophan is detected in the healthy leaves. It is presumed that such amino acids as tyrosine and phenyllanine are decreased by the incooperation of free amino acid to TMV protein in the process of the process of the leaf protein metabolism which is caused by TMV-RNA trapping action in the diseased leaf protoplasm. It is thought that the decrease of asparagine and the increase of asparic acid in the healthy leaves are the results of in incooperaton of NH2, produced by the protein dissimilation in the diseased leaves, to aspartic acid; it's reaction is caused by the respiration of the diseased leaves accelerated by TMV attack. It is presumed, consequently, that the check of the diseased tobacco leave growth is influenced by the reduction of such amino acids as tryptophane and glutamic acid, which reduction may be due to the abnormal protein metabolism and the action of certain enzyme caused by TMV attack on host protoplast.

• Archives of Plastic Surgery
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• 제38권4호
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• pp.539-542
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• 2011

Purpose: Despite the increasing number of patients with HIV (human immunodeficiency virus) infection, surgical experience with these patients remains limited in aesthetic and reconstructive surgery. The authors performed breast reconsruction with free TRAM (Transverse Rectus Abdominis Muculocutaneous) flap in HIV infected patient firstly in Korea. Methods: A 53-years-old female with HIV positive underwent delayed breast reconstruction with free TRAM flap and 6 months lateral nipple reconstruction was performed. All procedures were performed according to the HIV infection control guidelines provided by the Korea Centers for Disease Control and Prevention. Results: There were no complications such as infection, hematoma and flap loss and symmetry of breast was achieved. Conclusion: When the operation is performed in line with the guidelines of HIV infection control, breast reconstruction with free flap is possible and can obtain successful results.

• 마케팅과학연구
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• 제20권2호
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• pp.219-230
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• 2010

数字内容在给公司带来机遇和挑战的同时也极大的改变了我们的生活. 创意企业整合视频, 图片, 文本和数据进行数字化过的音频, 开发新产品或服务, 创作数字经验推广自己的品牌. 大多数有关数字内容的文献是关于基本概念或者营销的发展. 其实, 比起普通产品或服务的传统价值链, 数字内容产业似乎有更多的潜在价值. 因为相当多的数字内容是免费的, 价格, 作为信息的质量或价值的指标, 不是必须被感知的(Rowley 2008). 很显然, 当前数字内容的主题是 "价值" 和关于消费者对数字内容的感知价值的研究. 本文讨论了体验价值在消费者评估数字内容时的优势. 本文在对数字内容 "价值" 的理解方面有两个不同但是相关的贡献. 第一, 基于数字内容与普通产品和服务的比较, 本文提出了两个关键特点使得体验战略适合数字内容: 无形和接近于零的再造成本. 最重要的是, 基于对公司的理想化的价值和客户的感知价值之间的差异的讨论, 本文强调了数字内容的价格和定价与普通产品和服务的不同. 无形的结果是, 价格可能并不反映顾客感知价值. 另外, 数字内容的成本处在发展阶段可能非常高但再造会大幅缩水. 而且, 由于前面提到的价值鸿沟, 这个价格政策改变因不同的数字内容而不同. 例如, 平价战略通常用于电影和音乐(Magiera 2001;Netherby 2002), 而有持续的需求的数字内容如在线游戏和杀毒软件的问题牵涉到一个更复杂的效用和极具竞争力的价格水平. 数字内容企业必须探索各种各样的策略来克服这个缺口. 对于广告, 形象, 口碑等常用的市场战略和他们对顾客感知价值的影响的研究变得至关重要. 中国数字内容产业正变得越来越国际化, 并引起了具有各自竞争优势的国家和地区的关注. 2008-2009中国数字内容产业年度发展报告(CCIDConsulting 2009)表明, 在国内需求和政府政策的大力支持下, 中国数字内容产业在2008年保持了大约30%的快速增长, 表明了这个产业在明显的初期扩张阶段. 在中国, 需要更新的杀毒软件和其他软件程序使用季度定价政策. 用户可以免费下载试用版, 用6个月或一年. 如果他们更久的使用, 连续的付款方式是必要的. 他们在试用阶段检测数字内容的优良度, 决定是否要付继续使用. 对于中国的音乐和电影工业的发展战略, 体验最初没有被广泛的应用, 虽然其他国家的公司注意到体验的重要性并探索了相关的战略(如客户在下载前有好几秒可以免费听听音乐). 由于上述原因, 杀毒软件在中国可以代表数字内容产业而且在中国杀毒市场探索了体验价值在顾客的数字内容感知价值中的优势. 为了提高调查数据的可靠性, 该研究集中在那些有使用杀毒软件经验的人群. 实证结果显示, 体验价值对顾客对数字内容的感知价值有积极的影响. 换句话说, 因为数字内容是无形的, 再造成本几乎为零, 客户的评估是根据他们的体验. 另外, 形象和口碑不产生积极的影响, 只对体验价值有影响. 这就是说, 数字内容价值链不同于普通产品或服务. 体验价值有显著的优势并调节形象和口碑对感知价值的作用. 这个研究结果有助于了解为什麽在发展中国家存在免费的数字内容下载. 客户只有通过体验它才可以感知数字内容的价值. 这也是为什么政府如此扶持发展数字内容. 其他发展中国家在起步阶段的数字内容企业可以借鉴这里的建议. 另外, 基于体验战略的优势, 公司应该更努力投资于客户的体验. 由于数字内容的特点和价值鸿沟的存在, 顾客只有经历了他们真正想要的才能感知更多的无形的数字内容的价值. 而且, 因为再造成本近乎为零, 公司可以使用体验战略, 以提高客户对数字内容的理解.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.48-49
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• 2005

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 추계학술대회 발표논문 초록집
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• pp.161-161
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• 2003

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 1996년도 국제심포지움 21세기 첨단식물생산시스템의 실용화
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• pp.117-128
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• 1996

A garlic (Allium sativum L.) is an important and useful vegetable such that it is not only most used for spices but also exploited for health foods and pharmaceuticals. The garlic is a kind of vegetable propagated vegetatively through cloves. Therefore, development of new varieties of garlics is possible only through selection breeding because cross breeding is impossible. (omitted)