• Journal of Microbiology and Biotechnology
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• v.30 no.11
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• pp.1739-1749
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• 2020

Exopolysaccharide produced by the yeast Papiliotrema flavescens, isolated from wine grape berries of Champagne vineyard, was investigated for both chemical and functional characterization. SECMALLS and colorimetric assay analyses showed that the EPS is a high M_W heteropolymer (2.37 × 10⁶ g/mol) majorily consisting of mannose, glucose, xylose and glucuronic acid as monosaccharide constituents, with two substituents (sulphate and phosphate groups), and a minor protein moiety. Structural enchainment of these carbohydrates based on methylation, GC-MS and NMR analyses revealed a linear main backbone built up of α-(1 → 3)-D-mannopyranosyl residues on which are branched side chains consisting of a single β-D-glucopyranosyluronic acid residue and β-(1 → 2)-xylopyranoses (2-5 residues). Suggestion of some xylopyranose side chains containing a mannose residue at the nonreducing terminal end was also proposed. This is first report on EPSs from the grape P. flavescens yeast with such structural characteristics. Furthermore, investigations for valuating the application performance of these EPS in relation with their structural features were carried out in 8% alcohol experiment solutions. Very exceptional viscosifying and foaming properties were reported by comparison with commercial biopolymers such as Arabic, gellan and xanthan gums. The intrinsic properties of the natural biopolymer from this wild grape-associated P. flavescens yeast make it a potential candidate for use in various biotechnology applications.

• Research in Plant Disease
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• v.14 no.2
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• pp.90-94
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• 2008

To investigate the severity of crown gall disease on grapevine, the ratio of healthy vs. galled grapevines and the presence of the pathogen of the disease in soil were measured in Korean vineyards. In field and greenhouse cultivations, the crown gall incidence of the cv. Kyoho grapevines was $0.4{\sim}97.9%$ and $1.4{\sim}3.8%$ and those of cv. Campbell Early was $1.2{\sim}2.1%$ and $0{\sim}1.8%$, respectively. The higher populations of Agrobacterium spp. were isolated from soils of grapeveins with crown gall than from soils of noninfected vineyards. Based on the colony shapes and growth on plates, 480 isolates of Agrobacterium spp. from 21 soil samples were collected. Only 13 isolates out of 480 developed the gall on inoculated grapevines.

• Journal of Environmental Impact Assessment
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• v.22 no.6
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• pp.557-568
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• 2013

In this study, Calculated the nonpoint sources(NPS) load per unit area about various rainy events in vineyard of Nakdong River basin. NPS monitoring and calculation for NPS load per unit area were estimated from 'Investigation method of precipitation discharge(National Institute of Environmental Research, 2007)'. The evaluation of applicability for NPS load per unit by compared with prior research data and Total Maximum Daily Load(TMDL) data. Five target areas were each $2000m^2$, $1800m^2$, $1943m^2$, $2484m^2$, $864m^2$ and located in Gyeongsangbukdo Gyeongju, Gyeongsangbukdo Sangju, Gyeongsangnamdo Hapcheon in Korea. Since fruits were the only crop on the target area, the characteristics of stormwater discharge at survey sites could be evaluated independently. A total of 115 rainfall events in the Orchard area during five years(2008-2012) was surveyed, and 38 of them became stormwater discharge. In the Nakdong River watershed, average of event mean concentrations(EMCs) in Orchard area for biochemical oxyzen demand(BOD), Chemical oxyzen demand(COD), total nitrogen(T-N), total phosphorus(T-P) were 2.0mg/L, 10.1mg/L, 3.195mg/L, 0.578mg/L, respectively. NPS load per unit area in Orchard area showed BOD : $2.0kg/km^2{\cdot}day$, COD : $10.2kg/km^2{\cdot}day$, T-N : $3.220kg/km^2{\cdot}day$, T-P : $0.606kg/km^2{\cdot}day$.

• Korean Journal of Environmental Agriculture
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• v.33 no.3
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• pp.178-183
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• 2014

BACKGROUND: The white stain symptom of grape clusters and canes by dust-like particles occurred in many vineyards recently. This study was conducted to investigate the ecological characteristics of white stain symptom in grapevines and vineyards. METHODS AND RESULTS: Optimal climate condition for white stain symptom was $25^{\circ}C{\sim}30^{\circ}C$ with 60% of humidity. Moreover, closed condition with same humidity showed higher incidence rate of white stain symptom than ventilation condition. Grape varieties with black berry skin such as Campbell-Early and Kyoho were more sensitive to white stain symptom compare to varieties with green and red berry skin. Although the pathogens were not detected until March, they increased from April, and increased sharply from mid of July. The pathogens may overwinter in the infected stems and/or on the bark as a mycelium. According to the increase of sugar content of grape from August to September, the mycelium which was parasitic on the bark grew to move to the fruits through the stems, and finally reached the fruit stalk to detach berries from the clusters. CONCLUSION: Well ventilation is recommended inside the vineyard since mid-July with roll up an insect net. In addition, infected stems and fruits should be removed out from grapevines infected with pathogens in the vineyards.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.621-631
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• 1996

It is said that robot can be used for multi-purpose use by changing end effector or/and visual sensor with its software. In this study, it was investigated what multi-purpose robot for fruit-production was using a tomato harvesting robot and a robot to work in vineyard. Tomato harvesting robot consisted of manipulator, end-effector, visual sensor and traveling device. Plant training system of larger size tomato is similar with that of cherry-tomato. Two end-effectors were prepared for larger size tomato and cherry-tomato fruit harvesting operations, while the res components were not changed for the different work objects. A color TV camera could be used for the both work objects, however fruit detecting algorithm and extracted features from image should be changed. As for the grape-robot , several end-effector for harvesting , berry thinning , bagging and spraying were developed and experimented after attaching each end-effector to manipulator end. The manipulator was a polar coordinate type and had five degrees of freedom so that it could have enough working space for the operations. It was observed that visual sensor was necessary for harvesting, bagging and berry-thinning operations and that spraying operation requires another sensor for keeping certain distance between trellis and end-effector. From the experimental results, it was considered that multi-operations by the same robot could be appropriately done on the same or similar plant training system changing some robot components . One of the important results on having function of multi-operation was to be able to make working period of the robot longer.

• The Korean Journal of Pesticide Science
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• v.17 no.1
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• pp.20-26
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• 2013

This study was carried out of investigate the effect of weed control methods on the growth of 'campbell early' grape at the vineyards which was divided into the 18 zone ($3.5m{\times}5.5m/zone$) in the grape research institute. It's investigated control effects, growth and quantity of grape and dominant weed of July~August by sprayed glufosinate ammonium and paraquat dichloride each 3 times, fabric covering, grass planting (Festuca myuros), mechanical weeding (3 times). The weed showed total of 16 species at the vineyards. Late-May to early growth stage of grape was dominated Chenopodium album and Trifolium repens, but to late growth stage of grape from mid-July was dominated Erigeron canadensis, Echinochloa crus-galli and Chenopodium album. Weed control effect of 10 day after treatment showed fabric covering 100%, grass planting (Festuca myuros L.) 95.3%, mechanical weeding 81.9%, glufosinate ammonium (3 times) 98.1% and paraquat dichloride (3 times) 90.4%, respectively. Growth of grape was higher herbicides treatment and mechanical weeding than others. Yield tended to be higher glufosinate ammonium (3 times) and paraquat dichloride (3 times) each 12.6 kg/tree, 12.3 kg/tree than others.

• The Korean Journal of Pesticide Science
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• v.14 no.4
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• pp.440-445
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• 2010

This study was carried out to investigate the toxicities of five registered insecticides to the Lycorma delicatula nymphs and adults. Insecticidal activities were evaluated by testing systemic and residual effects in the laboratory, and control efficacy in the vineyard. For the 3rd nymphal instars and adults, etofenprox+diazinon, chlorpyrifos, etofenprox, dinotefuran and imidacloprid showed perfect insecticidal activity. The systemic effects of dinotefuran and etofenprox+diazinon on grape roots at half concentration were showed the adult mortality of 82.2% and 84.4%, respectively. Chlorpyrifos at recommended concentration was showed mortality of 86.0%. The leaf systemic effects at recommended and half concentrations of all insecticides except dinotefuran were lower than 65% but the mortality at double concentration of chlorpyrifos, dinotefuran, etofenprox were more than 82%. The residual effect between etofenprox+diazinon and dinotefuran at recommended and double concentrations were 100% at 14 days after treatment, the other insecticides have low efficacy. All the four insecticides showed 87% control value on nymphs in the field test and adults appeared more than 96% control value st and adinotefuran, etofenprox+diazinon, however, the other insecticides decreased to 59.1 % and 61.2%. Therefore, dinotefuran and etofenprox+diazinon showing high systemic effects to roots and long residual effects to leafs have high control efficacies.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.626-634
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• 2012

There are variations among grapevine genotypes in the levels of tolerance to cold, and cold-hardiness of grapevine has been affected by the change of short-term environment during over-wintering. In this study, the responses of vines to cold in open field and rain-shelter system were investigated to obtain useful information in increasing the tolerance to cold in grape cultivation. Total carbohydrate content of bearing mother branches was higher in the rain-shelter system than in the open field, and lower in the branches of 'Muscat Bailey A' than in 'Campbell Early' and 'Kyoho'. Bud-burst and shoot growth were better in the rain-shelter system than in open field, whereas there is no significant difference among the treatments of net beside vines. There was also low incidence of gray mold in rain shelter system. Stilbene compounds such as t-piceid, resveratrol, piceatannol, c-piceid were accumulated in the cold-treated shoot from vine cuts harvested in rain shelter system. Genes of chalcone isomerase, manganese superoxide dismutase, proline rich protein 2, and temperature induced lipocalin were highly expressed in the cold-treated shoot from vine cuts harvested in rain shelter system. While there was not change of air temperature, but high reduction of wind speed in the rain shelter system compared to open field, and increase in the reduction of wind speed by net treatment. The damage of grapevines by cold in the extreme low temperature could be reduced by keeping them in the rain shelter system with net during winter season.

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.113-118
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• 2015

The objective of this experiment was to evaluate the effects of application wide plastic shelter (WPS) in the 'Campbell Early' grape. WPS was developed 30cm wider than standard plastic shelter (SPS; 210cm). Under the plastic shelter, the average daily temperature was $2{\sim}3^{\circ}C$ higher than ambient temperature, but WPS treatment was $0.5{\sim}1^{\circ}C$ higher than SPS treatment. However, relative humidity 15~20% lower than ambient and shown different result between two treatments annually. The occurrence of brown leaf spot disease caused by Pseudocecospora vitis on the vineyard decreased 55.6% (2012) and 17.2% (2013) under WPS than SPS. Grape bunches collected two harvest seasons (2012, 2013), were analyzed fruit quality (total soluble solid; TSS, titratable acidity; TA, berry weight, berry size). In 2012, grapes had no differences in the quality of two treatments, but in 2013, significantly increase $0.4^{\circ}Bx$ of TSS and decrease 0.8% of TA on the WPS than SPS. It suggests that the application WPS reduced the incidence of brown leaf spot disease annually, and then affect to the fruit quality.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.1
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• pp.28-35
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• 2006

An accurate prediction of budburst in grapevines is indispensable for vineyard frost warning system operations in spring because cold tolerance depends heavily on phonology. However, existing frost warning systems utilize only daily minimum temperature forecasts since there is no way to estimate the site-specific phonology of grapevines. A budburst estimation model based on thermal time was used to project budburst dates of two grapevine cultivars (Kyoho and Campbell Early), and advisories were issued depending on phonology as well as temperature. A 'warning' is issued if two conditions are met: the forecasted daily minimum temperature falls below $-1.5^{\circ}C$ and the estimated phonology is within the budburst period. A 'watch' is issued for a temperature range of -1.5 to $+1.5^{\circ}C$ with the same phonology condition. Validation experiments were done at 8 vineyards in Anseong in spring 2005, and the results showed a good agreement with the observations. This method was applied to the climatological normal year (1971-2000) to determine sites with high frost risk at a 30 m grid cell resolution. Among 608,585 grid cells constituting Anseong, 1,059 cells were identified as high risk for growing Kyoho and 2,788 cells for Campbell Early.