• Korean Journal of Microbiology
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• v.24 no.2
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• pp.147-153
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• 1986

Viable potential of Mycobacterium smegmatis, a slow grower in vitro cultivation and of M. leprae, an obligate intracellular parasitic bacterium, which can not be cultured yet in vitro was assessed by fluorospectrophotometry. Bacterial cells in different numbers and under various physiological status were incubater with fluorescein diacetate(FDA). After an incubation of the bacterial preparations with FDA at specified conditions, amount of fluorescein inside bacteria was measured by a fluorospectrophotometer at 470nm and 510nm of excitation and emission wavelengths, respectively. Fluorounit given by such bacteria showed a correlation with assessment of viability of the same preparations made by other methods, such as optical density and colony forming units of M. smegmatis and intracellular ATP content of M. leprae. The possible use of fluorospectrophotometry in assessing viability or physiological potential of bacteria, particularly intracellular parasites and fastidious organisms to culture in vitro is discussed in relation to other methods.

• Journal of Environmental Impact Assessment
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• v.22 no.5
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• pp.525-528
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• 2013

River otter(Lutra lutra) are listed as endangered species and Natural monument in Korea, and this study examined the possibility of extinct of river otter in Hongchon river using with the application of Population Viability Analysis (PVA) technique. In Hongchon river areas population was estimated 9 individuals for the last 1999-2005 years and PVA analysis was done for the next 10 years using the average population of 9. Using the initial population the river otter was estimated 30% of extinct for the next 10 years. This estimation was quite low considering water pollution and construction of highways. Also PVA only used population size lacking in other life history information. Nonetheless river otter population can be in risk of extinction if the current construction of crossovers, cement bank are maintained. Long term information regarding life history needs essential.

• The Korean Journal of Nuclear Medicine
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• v.39 no.2
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• pp.133-140
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• 2005

The potential for recovery of left ventricular dysfunction after myocardial revascularization represents a practical clinical definition for myocardial viability. The evaluation of viable myocardium in patients with severe global left ventricular dysfunction due to coronary artery disease and with regional dysfunction after acute myocardial infarction is an important issue whether left ventricular dysfunction may be reversible or irreversible after therapy. If the dysfunction is due to stunning or hibernation, functional improvement is observed. but stunned myocardium may recover of dysfunction with no revascularization. Hibernation is chronic process due to chronic reduction in the resting myocardial blood flow. There are two types of myocardial hibernation: "functional hibernation" with preserved contractile reserve and "structural hibernation" without contractile reserve in segments with preserved glucose metabolism. This review focus on the application of F-18 FDG and other radionuclides to evaluate myocardial viability. In addition the factors influencing predictive value of FDG imaging for evaluating viability and the different criteria for viability are also reviewed.

• Journal of Ocean Engineering and Technology
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• v.22 no.3
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• pp.47-57
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• 2008

Many accidents occur on the oceans. Accidents involving ships can cause enormous damage. When an accident occurs, the cost of environmental restoration can bankrupt even a top-ranked international company. The potential damages resulting from ship accidents have resulted in many international agreements such as MARPOL, SOLAS, ISM Code, etc. The viability of damaged ships has become more and more important. A damaged ship's viability and cargo safety can be improved by a safety assessment design procedure. We consider th￡ shipbuilding industry's distributed characteristics to define a safety assessment system with software engineering-based requirements analysis. We used a distributed network-based simulation method (HLA - High level architecture), because of ease of reuse and expansion of existing components to other situations, and because it is a military standard. HLA is the standard for distributed network-based simulation in many countries including the United States, Canada, Israel, and Korea. The paper describes research to develop a prototype of a network-based safety assessment simulation system by software engineering based analysis.

• Journal of Embryo Transfer
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• v.32 no.3
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• pp.221-226
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• 2017

Spermatozoa viability can be assessed by microscopy, flow cytometry, and other methods using fluorescent stain. Flow cytometry can be used to examine the morphological and functional characteristics of spermatozoa in a short time. The purpose of this study was to compare the viability of cryopreserved spermatozoa in Jeju black cattle by two dual fluorescent stain methods. Semen of Jeju black cattle raised in Subtropical Livestock Research Institute, National Institute of Animal Science, RDA were collected with artificial vaginal technique. Sperm was diluted with $Triladyl^{(R)}$-egg yolk diluent and then was performed cryopreservation. There was no significant difference in viability of spermatozoa according to the two dual fluorescent stain methods. However, when the distribution of spermatozoa according to the staining method was compared, the spermatozoa group stained with 6-CFDA/PI was more clearly distinguished than the spermatozoa group stained with calcein AM/PI.

• Journal of Environmental Impact Assessment
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• v.18 no.5
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• pp.293-299
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• 2009

Wintering migratory species of white-fronted geese (Anser albifrons) are common visitor in South Korea, and this study examined the survivorship of white-fronted geese in the Junam Reservoir with the application of Population Viability Analysis (PVA) technique. In Junam PVA analysis was done for the next 50 years using factors of breeding population, sex ratios, survivorship in the VORTEX program. As a result white-fronted geese will reach to carrying capacity within 40 years, and population will reach to carrying capacity later when it becomes smaller. Also with a large initial population white-fronted geese will reach to carrying capacity earlier. In conclusion, for the next 50 years the white-fronted geese are expected to a long-term survival with stable condition with survivorship (0.03) and extinction rate (0.0).

• Journal of Sensor Science and Technology
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• v.32 no.6
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• pp.412-417
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• 2023

There is increasing interest in the rapid and highly sensitive monitoring of cell viability in biological and toxicological research. Conventional methods depend on optical assays using Water Soluble Tetrazolium-8 (WST-8) or 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, which requires a large volume of samples and special instruments, necessitating shipment of clinical samples to laboratories. This paper reports on the development of a rapid and sensitive electrochemical (EC) sensor using screen printed electrode (SPE) and surface modification using 4'-mercapto-N-phenylquinone diamine (4'-NPQD), as double electron mediators, for monitoring cell viability via the measurement of nicotinamide adenine dinucleotide (NADH). We used the sensor to observe the viability of MCF-7 and doxorubicin (Dox)-treated cells. The oxidation current of NADH was measured via chronoamperometry (CA), and the EC results showed a good linear relationship when compared with NADH quantification using WST-8 assay. The analysis time was only 10 s and limit of detection (LOD) of NADH was 1.78 µM. Our EC method has the potential to replace conventional WST assays for cell viability and cytotoxicity experiments.

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.04a
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• pp.21-21
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• 2019

• KIEAE Journal
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• v.11 no.2
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• pp.45-57
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• 2011

As climate change and environmental degradation become more evident, the public's concern and knowledge on sustainable development requires planners to have regards to social impacts and long term economic viability, as well as environmental impacts. The main objective of this study is to analyze existing sustainability assessment tools for single detached houses. The most widely used oversea tools are selected. From analysis on the existing assessment tools, "Impact Estimator" by Athena Institute, "Bees 4.0" by NIST, "CASBEE for Home" by JSBC, "Envest for Housing" by Energy Saving Trust, "LISA" by Center for Sustainable Technology, "SBTool" by iiSBE (International Initiative for Sustainable Built Environment), it can be seen that the current sustainability assessment programs are in need of much improvement. The selected programs are analyzed on scope of program setting & running, data input & output, assessment categories, and strength & weakness. This study is to supplement the existing sustainability assessment tools for single detached houses, and provide information for the development of web-based program to assist architects or home owners to plan sustainable houses in Korea in the schematic design stage.

• The Korean Journal of Nuclear Medicine
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• v.27 no.2
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• pp.155-160
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• 1993

The identification of viable myocardium in patients with coronary artery disease and left ventricular dysfunction is an issue of increasing clinical relavance in the current era of myocardial revascularization. There are at least two forms of reversible myocardial dysfunction. Early reperfusion does not always lead to immediate functional improvement; rather, the return of contractility in tissue salvaged by reperfusion is delayed for hours, days or even weeks, a phenomenon that has been termed "stunned myocardium". Some patients with coronary artery disease show myocardial dysfunction at rest which are associated with reduced perfusion, and which disappear after revascularization; this phenomenon has been termed "hibernating myocardium". Recently, cardiac imaging techniques that evaluate myocardial viability on the basis of perfusion-contraction mismatch and inotropic reserve have gained substantial popularity and clinical success. This review focus on the application of $^{201}TI$ and $^{99m}Tc-MIBI$ to address myocardial viability in patients with hibernating and stunned myocardium. It is clear that 4-hour redistribution images of $^{201}TI$ underestimate ischemia and overestimate scar. Delayed imaging and reinjection imaging have been developed for the assessment of viability. Among many protocols suggested, stress-redistribution-reinjection imaging gained most popularity. Although $^{99m}Tc-MIBI$ could identify myocardial viability, $^{201}TI$ reinjection technique was regarded as superior to it. In conclusion, $^{201}TI$ stress, 4-hr rest redistribution, and reinjection imaging technique may be the most preferable method for evaluation of myocardial viability.