Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Comparison of Two Fluorescent Stain Methods for Jeju Black Cattle Spermatozoa Viability Assessment by Using Flow Cytometry

제주흑우 정자 생존성 평가를 위해 flow cytometry를 사용한 두가지 형광 염색법의 비교

  • Shin, Sang-Min (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Park, Seol-Hwa (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Son, Jun-Gyu (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Cho, In-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Seong, Pil-Nam (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Kim, Nam-Young (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Woo, Jai-Hoon (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Shin, Moon-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Park, Nam-Geon (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
  • 신상민 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 박설화 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 손준규 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 조인철 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 성필남 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 김남영 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 우제훈 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 신문철 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 박남건 (농촌진흥청 국립축산과학원 난지축산연구소)
  • Received : 2017.07.11
  • Accepted : 2017.09.15
  • Published : 2017.09.30
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Abstract

Spermatozoa viability can be assessed by microscopy, flow cytometry, and other methods using fluorescent stain. Flow cytometry can be used to examine the morphological and functional characteristics of spermatozoa in a short time. The purpose of this study was to compare the viability of cryopreserved spermatozoa in Jeju black cattle by two dual fluorescent stain methods. Semen of Jeju black cattle raised in Subtropical Livestock Research Institute, National Institute of Animal Science, RDA were collected with artificial vaginal technique. Sperm was diluted with $Triladyl^{(R)}$-egg yolk diluent and then was performed cryopreservation. There was no significant difference in viability of spermatozoa according to the two dual fluorescent stain methods. However, when the distribution of spermatozoa according to the staining method was compared, the spermatozoa group stained with 6-CFDA/PI was more clearly distinguished than the spermatozoa group stained with calcein AM/PI.

Keywords

References

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