• Journal of Plant Biology
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• 제37권4호
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• pp.421-428
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• 1994

The vegetative and male reproductive anatomy of a marine alga, Laurencia intercalaris sp. nov. (Rhodomelaceae, Rhodophyta), is described from subtidal habitats of eastern and southern Korea. This species has terete thalli with entangled fibrous holdfasts and regularly alternate branching of ultimate branchlets, and is inseparable from L. okamurae Yamada on the basis of habit. Vegetative axial cells produce a trichoblast and four pericentral cells in an alternating sequence. Spermatangia are produced intercalary or subterminally from one of two laterals on suprabasal cells of trichoblasts arising from axial cells in apical pits of branchlets. The other lateral remains sterile. In this sterile lateral, budding-like regeneration occurs on older segments that are oabscised. Comparison is made with other related Laurencia species, particularly those with terete thalli. The vegetative anatomy and the regeneration in sterile laterals of male trichoblasts, with the mode of spermatangial formation, distinguish the new species from previously described species of Laurencia including L. okamurae.

• ALGAE
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• 제18권4호
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• pp.333-339
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• 2003

To establish a cultivation method of Petalonia fascia, seeds and seedlings cultures and growth tests were performed at the Daeri aquafarm in Haeui, Shinan, Jeollanamdo, Korea. Gametes were easily released from the mature plurilocular sporangia. They developed to crustose discoidal stolons and grew to filamentous and discoidal stolons. The indoor seeding was performed by using the 100-150 ${\mu}m$long fragments of stolons on Porphyra nets and the erect thalli developed from the cuttings when the seawater temperatures were 10-15$^{\circ}C$. In the experimental cultivation in the sea, 1-2mm long plantlets were found after 15 days of cultivation; after two months thalli grew to their maximal size of 215-355 mm long blades; after three months the length of thalli began to decrease due to distal disintegration and the plant color changed to yellow and epiphytic diatoms were attached on the thalli, which deteriorated the quality of products. The cultivation of P. fascia by the regeneration of filamentous-discoidal complexes was carried out successfully for the first time in Korea.

• 한국생태학회:학술대회논문집
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• 한국생태학회 1999년도 국제 Symposium 및 '99 춘계 공동 학술 발표대회
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• pp.97.2-97.2
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• 1999

• ALGAE
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• 제20권2호
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• pp.141-150
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• 2005

To make a preliminary identification of the gracilarioid plant attached to cultivation ropes of Undaria pinnatifida and establish a method of cultivating this plant, the first taxonomic and cultivation studies on this species in Korea were conducted. This gracilarioid plant was identified from its morphological and anatomical features, as Gracilaria chorda. Growth tests using the 10, 20, and 30 cm cuttings of axes of G. chorda were performed twice, from May 3 to August 21, 2002 and from December 15, 2002 to April 3, 2003 in Ihoijin aquafarm, Hoijin, Jangheung, Jeollanamdo, Korea. In the first growing test, the thallus length of the 10, 20, and 30 cm cuttings increased twelve-fold, ten-fold, and seven-fold; the wet weight increased 81-fold, 60-fold, and 41-fold; the numbers of more than 10 cm-long branches increased 3.8-fold, 5.2-fold, and 6.1-fold, respectively. In the second growth test, the thallus length of the 10, 20, and 30 cm cuttings increased seven-fold, 5.5-fold, and four-fold; the wet weight increased 81-fold, 53-fold and 36-fold; the number of branches increased 3.8-fold, 7.3-fold, and 6.6-fold, respectively. The cultivation of G. chorda by vegetative regeneration using cuttings of thallus axes was successful for the first time in Korea.

• 한국생태학회:학술대회논문집
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• 한국생태학회 2002년도 VIII 세계생태학대회
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• pp.133-139
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• 2002

Regeneration process of the Korean beech (Fagus multinervis Nakai) forest was studied in relation to the distributional pattern and age structure. In this study, it was clarified that the Korean beech forest is regenerated in both way: seedlings(sexual reproduction) and sprouts(vegetative reproduction) formations. In the large gaps, seedling may be established, resulting in clusters of even-aged individuals. But in the small-sized gaps, there may filed by the extention of surrounding trees and/or by the young trunks, which have sprouted from the base of stumps. As most gaps in the F. multinervis forest were derived from falling of a single stem, regeneration by sprouts seems to be more common than that by the seedlings.

• The Korean Journal of Ecology
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• 제25권4호
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• pp.267-273
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• 2002

Regeneration process of the Korean beech (Fagus multinervis Nakai) forest was studied in relation to the distributional pattern and age structure. In this study, it was clarified that the Korean beech forest is regenerated in both way: seedlings(sexual reproduction) and sprouts(vegetative reproduction) formations. In the large gaps, seedling may be established, resulting in clusters of even-aged individuals. But in the small-sized gaps, there may filled by the extention of surrounding trees and/or by the young trunks, which have sprouted from the base of stumps. As most gaps in the F. multinervis forest were derived from falling of a single stem, regeneration by sprouts seems to be more common than that by the seedlings.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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• pp.6-9
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• 2005

Plant biotechnology involving genetic modification has been rather controversial. However, the major issues related to safety are being addressed by continued improvements in technology. Some of the related facts will be highlighted to set the tone for a scientific discussion on the possibilities of using the technology for crop improvement. Our main research interest is to understand the molecular regulation of shoot bud regeneration in plant tissue culture, which is essential for crop improvement by biotechnology. We have isolated and characterized some genes that are associated with adventitious shoot regeneration. These include a MADS-box cDNA (PkMADS1) from paulownia kawakamii, which regulates vegetative shoot development and in vitro shoot regeneration from leaf explants. Another gene we have characterized from petunia codesfor a cytokinin binding protein (PETCBP). Preliminary functional analysis of this gene indicated that this also affects adventitious shoot bud initiation. Also, the antisense suppression of this gene in petunia causedexcessive branching. Results from our work and selected other publications will be used to highlight the possibilities of manipulation of such genes to improve crop species.

• 한국산림과학회지
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• 제84권3호
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• pp.394-405
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• 1995

온대 삼림지대에서 산화 적지나 개벌 적지에 왕성하게 번성하는 것이 ericaceous속이다. ericaceous속의 식물들은 성장이 빨라서 다른 종과의 경쟁에서 유리하고 또한 allelopathy 효과 때문에 침엽수 갱신에 영향을 미친다. Kalmia가 번성하는 지역에서는 삼림 갱신이 잘 되지 않거나 조림한 침엽수의 생장이 저조하였다. 이러한 생상 저해 현상은 서유럽의 Calluna - Sitca spruce - Scots pine 생태계와 미국의 북서 태평양 지역의 Gaultheria - cedar/hemlock 생태계에서도 나타나고 있다. 삼림이 파괴된 다음에 Kalmia - black spruce 생태계의 다양한 변화는 침엽수 생장을 저해하거나 갱신을 방해하는 원인으로 생각되어지고 있다. 양분경쟁에 대한 논쟁의 여지는 있으나 Kalmia는 black spruce 생장을 억제하는 주원인으로 작용한다. 여러 곳의 야외, 실내 및 온실에서 한 실험 결과는 이러한 결론을 뒷받침해 주고 있다. 본 논문에서는 Kalmia 잎으로부터 8가지의 폐놀물질을 분리동정해 냈으며 이들 중 4가지는 black spruce에 고도의 독성을 나타냈다. Kalmia 우점지역에서 allelopathic 영향을 적게 하므로써 black spruce의 갱신을 증진시키기 위한 여러 가지 방법에 대한 고찰을 하였다.

• 식물조직배양학회지
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• 제21권5호
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• pp.293-297
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• 1994

미나리아재비의 약을 배양하여 미숙화분 유래의 식물체를 획득하는데 있어서 생장조절제의 종류와 농도 및 배양 적기를 구명하기 위해 화분의 발육단계별로 캘러스, 배발생및 식물체 재분화에 미치는 영향을 조사하였다. 캘러스 및 배발생에는 2,4-D 및 kinetin 처리보다 NAA 및 BA처리에서 효과적이었으며 특히 0.5 mg/L NAA와 1.0 mg/L BA 처리구에서 가장 왕성하였다. 화분의 발육과정중 1핵성과 초기2핵성의 pollen mitosis 전후기인 개화 3일전의 약의 발육상태가 캘러스, 배발생 및 식물체 재분화에 적합하였다. 화분유래의 배발생은 영양세포의 계속적인 분열에 의해 이루어졌다.

• 한국수산과학회지
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• 제54권3호
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• pp.311-317
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• 2021

Growth differences in Caulerpa okamurae thallus parts and the effects of apex removal on growth were examined. Erect fronds, stolons, and erect fronds with stolons (erect+stolon) were grown for 14 days, whereas erect fronds without and with apex removal (-A and +A fronds, respectively), were grown for 25 days. The relative growth rates (RGR) of C. okamurae thallus parts ranged from 1.91% to 4.93% day^-1 with maximal and minimal RGR for the stolon and erect+stolon treatments, respectively. Over the 25 days in culture, the -A fronds showed a higher RGR (2.48% day^-1) than the +A fronds (1.96% day^-1). More new erect fronds and stolons were produced by -A fronds than those by +A fronds. In addition, basal cutting of erect C. okamurae fronds resulted in a greater plant length increase than that obtained from top cutting. These findings indicate that vegetative growth is common in C. okamurae, which has excellent wound healing ability, and cutting damage to erect fronds may stimulate enhanced vegetative growth. Thus, we suggest that stolons and erect fronds of C. okamurae could be used as seeding materials for large scale cultivation. Furthermore, this edible species could be a potential seaweed resource for rapid skin regeneration cosmetics.