• Title/Summary/Keyword: vegetative growth

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Enoylpyruvate Transferase Isozymes in Bacillus megaterium

  • Choi, Seung-Tae;Katsuji Tani;Ryoka Matsuno
    • Journal of Life Science
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    • v.2 no.4
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    • pp.232-239
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    • 1992
  • UDP_GlcNAc is metabolized to form vegetative cell wall, cortical peptidoglycans, and outermost layer consisting of galactosamine-6-phosphate ploysaccharide in life cycle of Bacillus megaterium. To obtain a better understanding of the UDP-GlcNAc regulation, we examined the activity of the common first enzyme for the synthesis of nucleotide precursors of peptidoglycans, enoylpyruvate transferase by newly developed method. Both the specific and the total activity decreased after the end of exponential growth followed by and increase from t5 but decreased again parallel to the appearance of the activity of UDP_GlcNAc-4-epimerase. Antibody specificity to anti-transferase IgG and the elution profile on DEAE-Sepharose revealed that B. megaterium has at least two enoylpyruvate transferase isozymes, and UDP_GlcNAc was metabolized to vegetative cell wall and cortical peptidoglycan by each isozme in exponential growth and in sporulation, respectively in life cycle.

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A Study on the Lysis of a Bluegreen Alga Anabaena cylindrica by a Bacterium (세균에 의한 남조 Anabaena cylindrica의 분해에 대한 연구)

  • Kim, Cheol-Ho;Gwon, O-Seop;Lee, Jin-Ae
    • ALGAE
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    • v.18 no.4
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    • pp.355-360
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    • 2003
  • A Gram (-), rod-shaped bacterium in size of 1.6-2.8 $\times$ 0.4 μm was isolated from a eutrophic reservoir, which exhibited growth-inhibiting effect against a bluegreen alga (Anabaena cylindrica). This isolate showed positive reactions for catalase and oxidase, and optimal conditions of 35-40°C and pH 9.0. This isolate was designated AC-1 in this manuscript. In a mixed-culture of A. cylindrica and AC-1, their growth patterns were inversely correlated and the bluegreen algal vegetative cells completely disappeared within 24-36 hours. AC-1 showed similar lytic activity in natural water as in an artificial medium. The lytic activity of AC-1 was dependent on the photosynthetic activity of A. cylindrica. When observed under phase contrast microscope, the isolate lysed vegetative cells of A. cylindrica in scattered state in a liquid medium, whereas heterocysts have not been lysed.

Growth Rate and Colony Morphology of Progenies of Zoospores and Oospores of Phytophthora cactorum causing Phytophthora rots in Apple Trees (과수목(果樹木)의 역병(疫病)을 유발하는 Phytophthora cactorum의 유주자(遊走子)와 난포자(卵胞子)의 균사생장과 균총의 형태)

  • Shim, Jae-Ouk;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.22 no.4
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    • pp.281-285
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    • 1994
  • Zoospore progenies of Phytophthora cactorum were relatively uniform and similar to their respective parent in the rate of linear extension, whereas oospore progenies were greatly various. Also, the character of colony pattern was quantitatively various in oospore progenies but not zoospore progenies. Therefore, these results suggested that multiple genes were involved in determining growth rate and colony morphology of P. cactorum, and support the hypothesis that species of Phytophthora are diploid during the vegetative phase.

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Spectral Reflectance Signatures of Major Upland Crops at OSMI Bands

  • Hong, Suk-Young;Rim, Sang-Kyu;Jung, Won-Kyo
    • Proceedings of the KSRS Conference
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    • 1998.09a
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    • pp.370-375
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    • 1998
  • Spectral reflectance signatures of upland crops at OSMI bands were collected and evaluated for the feasibility of crop discrimination knowledge-based on crop calendar. Effective bands and their ratio values for discriminating corn from two other legumes were defined with OSMI equivalent bands and their ratio values. June 22 among measurements dates was the best date for corn discrimination from two other legumes, peanut and soybean, because all OSMI equivalent bands and their ratio values in June 22 were highly significant for corn separability. Phenological growth stage of a silage corn (rs510) could be estimated as a function of spectral reflectance signatures in vegetative stage. Five growth stage prediction models were generated by the SAS procedures REG and STEPWISE with OSMI equivalent bands and their ratio values in vegetative stage.

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Thermosensing of Thermotactic Mutants, Dictyostelium discoideum Amoebae in Vegetative Stage

  • Hong, Choo-Bong
    • Journal of Plant Biology
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    • v.26 no.3
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    • pp.131-139
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    • 1983
  • Temperature response of amoebae of thermotactic mutants have been investigated. Amoebae of the mutant strain HO 428 showed positive thermotaxs which is strong at lower temperaturs and drops sharply above the growth temperature of amoebae. The temperature response of HO 428 amoebae was not affected by the length of amoebae on the grdients. HO 596 amoebae seemed to have both positive and negative thermotactic responses shortly after food depletion. Longer exposure of these amoebae on the thermal gradients induced a stronger negative response at lower temperatures and an apparent positive response at higher temperatures. A similar changes could be observed in HO 1445 amoebae. Based on the steady positive thermotactic response by HO 428 amoebae and the mode of change in termperature response at higher temperatures, 24$^{\circ}C$ and $25^{\circ}C$, by HO 596 amoebae, a model for the temperature response of vegetative Dictyostelium discoideum amoebae, strain HL 50, has been proposed. The main features of the model are: a positive response at the thermal gradients with midpoint temperatures lower than the growth temperatures of amoebae and a negative response above it.

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Effects of Simulated Sulfuric and Nitric Acid Rain on Growth and Seed Germination of Arabidopsis thaliana (인공 황산비 및 질산비가 애기장대의 생장과 종자발아에 미치는 영향)

  • 이석찬;박정안;박종범
    • Journal of Environmental Science International
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    • v.12 no.6
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    • pp.659-664
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    • 2003
  • The experiment was carried out to investigate the effects of sulfuric acid and nitric acid among the main components of simulated acid rain (SAR) on the growth of vegetative organs and seed germination of Arabidopsis thaliana. The Arabidopsis treated with SAR supplemented with sulfuric and nitric acids, respectively, showed 28% and 30% decrease of shoot and root growth compared to the control plants, and also many necrotic spots on leaf surfaces after SAR treatment were observed. The shoot and root length for plants grown with nitric acid rain was 14% and 17% lower, respectively, compared to the control, whereas those grown with sulfuric acid rain was 24% and 25% lower than control plants. When Arabidopsis seeds were sown in distilled water, germination rate was 100% after 7 days. However, 80% in SAR medium supplemented with sulfuric and nitric acids, 88% in sulfuric acid rain medium and 93% in nitric acid rain medium. The germination abilities of seeds harvested from SAR supplemented with sulfuric and nitric acids, sulfuric acid rain, and nitric acid rain were 73%, 73% and 94%, respectively. Consequently, sulfuric acids showed more inhibitory effects than nitric acids on the growth of vegetative organs as well as germination rates in Arabidopsis.

Proteases and Protease Inhibitors Produced in Streptomycetes and Their Roles in Morphological Differentiation

  • KIM DAE WI;KANG SUNG GYUN;KIM IN SEOP;LEE BYONG KYU;RHO YONG TAIK;LEE KYE JOON
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.5-14
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    • 2006
  • Streptomycetes are Gram-positive microorganisms producing secondary metabolites through unique physiological differentiation [4]. The microbes show unusual morphological differentiation to form substrate mycelia, aerial mycelia, and arthrospores on solid medium [19]. Substrate mycelium growth is sustaining with sufficient nutrients in the culture medium. The concentration of a specific individual substrate in the culture environment is the most important extracellular factor allowing vegetative mycelia growth, where extracellular hydrolytic enzymes participate in the utilization of waterinsoluble substrates. However, with starvation of nutrients in the culture medium, the vegetative mycelia differentiate to aerial mycelia and spores. It has been considered that shiftdown of essential nutrients for mycelia growth is the most important factor triggering morphological and physiological differentiation in Streptomyces spp. Since proteineous macromolecule compounds are the major cellular components, these are faced to endogenously metabolize following a severe depletion of nitrogen source in culture nutrients (Fig. 1). Various proteases were identified of which production was specifically related with the phase of mycelium growth and also morphological differentiation. The involvement of proteases and protease inhibitor is reviewed as a factor explaining the mycelium differentiation in Streptomyces spp.

A Comparison of Vegetative Growth of Kentucky Bluegrass and Perennial Ryegrass Cultivars in Different Levels of Shade (그늘하에서의 Kentucky Bluegrass와 Perennial Ryegrass 품종의 생육비교)

  • 구자형;김태일;원동찬;송남현;송천영
    • Asian Journal of Turfgrass Science
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    • v.5 no.1
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    • pp.23-32
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    • 1991
  • The purpose of this study was to determine the vegetative growth of two cool season turfgrasses during summer season in Korea. Ten respective cultivars of Kentucky bluegrass [Poapratensis L.] and perennial ryegrass [Lolium perenne L.] were exposed to unshaded, 30% and 60% shade for 4odays. First mowing height was 7cm, and then clippings were harvested every ten days at the same height. Shading increased the total length of clippings of all cultivars of two turfgrasses, but fresh and dry weight were significantly decreased. The highest shoot length obtained at 30% shade in Kentucky bluegrass and at 60% shade in perennial ryegrass. 'Rugky', 'Sydsport', 'Wabash' and 'Baron' of Kentucky bluegrass and 'Repel', 'Omega II', 'Ovation', 'Linn', 'Bell' and 'Manhattan' of perennial ryegrass showed relatively high tolerance to shade. Especially, in 'Citation II' perennial ryegrass, fresh and dry weight were increased by 30% shade. The number of tillers generally decreased with increasing shade. In 30% shade, 'Midnight' Kentucky bluegrass and 'Omega II' perennial ryegrass were least reduced and 'Baron' Kentucky bluegrass and 'Pennant' perennial ryegrass were most reduced. The cultivars of lower growth habit in full sunlight showed shade tolerance compared to those of higher growth habit.

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Effect of nutrient and moisture on the growth and reproduction of Epilobium hirsutum L., an endangered plant

  • Lee, Eung-Pill;Han, Young-Sub;Lee, Soo-In;Cho, Kyu-Tae;Park, Jae-Hoon;You, Young-Han
    • Journal of Ecology and Environment
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    • v.41 no.10
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    • pp.281-289
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    • 2017
  • Background: In this study, the growth and reproductive response of seedlings, grown in plastic pots with sand, to moisture and nutrients were analyzed in order to study the environmental conditions required to create an alternative habitat for Epilobium hirsutum L., an endangered plant. Results: Vegetative and reproductive growths of Epilobium hirsutum L. are accelerated with increase in moisture and organic matter content in the soil. Among vegetative organs, the number of runners related to asexual reproduction was the highest when the moisture content was over 25% and nutrient content between 7 and 14% in the soil. But the number of flowers related to flowering responses, among reproductive organs, was the highest when the moisture content was maintained at 75% and when nutrient content was 21% in the soil. The number of seeds, related to sexual reproduction, was the highest when the moisture content was over 25% and nutrient content between 14 and 21%. Conclusions: The study results show that a place with high moisture and nutrient content in the soil is advantageous to asexual and sexual reproduction of Epilobium hirsutum L. Therefore, we must serve periodically nutrient and seeds to sustain population in in situ conservation. Furthermore, it is advisable to create in riverside where abundant nutrient content have, making alternative habitat of Epilobium hirsutum L. Also, we must find species that have high contribution degree index through vegetation survey.

Ecological Studies on Heading of Rice Plant I. The Response to Photoperiod of Major Rice Varieties (수도의 출수생태에 관한 연구 제1보 주요수도 품종의 일장반응)

  • 최경구;장영남;이성춘
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.151-163
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    • 1983
  • This study was conducted to investigate the responses to photoperiod of some Korean rice cultivars at four levels of the day length, 10, 12, 14 and 16 hours. Generally the days to heading were reduced in the short day length. Japonica varieties seemed to have shorter basic vegetative growth phase and, were more sensitive to day length, while Japonica crossed Indica varieties showed longer basic vegetative growth phase and, were less sensitive to day length. All tested varieties could be classified into 19 ecotypes based on the periods of the basic vegetative growth phase and the sensitivity to day length.

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