• Parasites, Hosts and Diseases
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• v.48 no.3
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• pp.237-243
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• 2010

Comprehensive quarterly serosurveillance on scrub typhus in small mammals collected from military training sites located near the Demilitarized Zone (DMZ), northern Gyeonggi-do (Province), ROK was conducted to determine the potential rodent-borne and associated ectoparasite disease risks to military personnel. A total of 1,196 rodents and insectivores representing 8 species, Apodemus agrarius (87.3%, n = 1,044), Mus musculus (5.4%, n = 65), Crocidura lasiura (3.3%, n = 40), Microtus fortis (2.6%, n = 31), Micromys minutus (0.3%, n = 4), Tscherskia triton (0.3%, n = 4), Rattus norvegicus (0.3%, n = 4), and Myodes regulus (0.3%, n = 4) were assayed for the presence of antibodies to Orientia tsutsugamushi. O. tsutsugamushi antibodies were detected in 6 of 8 species and seroprevalence determined; A. agrarius (45.6%), M. musculus (23.1%), M. fortis (48.4%), M. minutus (50.0%), T. triton (50.0%), and R. norvegicus (25.0%). A total of 31,184 chigger mites collected from 508 rodents and insectivores were slide-mounted and 10 species belonging to 4 genera were identified. Leptotrombidium pallidum (53.4%) was the most frequently collected, followed by L. pal pale (15.7%), Neotrombicula tam/yai (14.3%), L. orientate (10.7%), L. zetum (3.1%), Walchia fragilis (2.1%), and L. gemiticutum (0.8%), while the remaining 3 species, L subintennedium, N. gardellai, and Euschoengastia koreaensis were rarely observed (prevalence < 10%). In contrast to previous surveys, higher chigger indices of the primary scrub typhus vectors, L. pallidum (165.4), L. orientale (45.0), and L. palpate (21.4), were observed during the spring season.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.235-240
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• 2002

We report the new method for the screening of genetically modified potato by competitive duplex-PCR using the potato specific single oligomer primer for the internal control and CaMV 35S promoter or NOS terminator specific primers. The single oligomer primer (rAGU4A) amplify the potato specific internal control band from the homozygous potato genomic DNA in the RAPD profiles of all analyzed potato varieties. The 530 bp internal control DNA was amplified independently to CaMV 35S promoter or NOS terminator DNA and identified as repetitive or microsatellite DNA of potato (AF541972). With this new technique, the transgenic potatoes which were transformed with vectors contained the different foreign genes are analyzed. In case of the commercialized transgenic potato varieties, 'Hew Leafs', those two genetic factors are used for promoter and terminator respectively So, this new PCR technique should be a promising method of cost effective and accurate screening for the commercialized GM potatoes on market.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.10C
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• pp.933-941
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• 2006

In this paper, we proposed an efficient coding method for digital hologram (fringe pattern) acquired by a CCD camera or by computer generation using multi-view prediction technique and MPEG video compression standard technique. It proceeds each R, G, or B color component separately. The basic processing unit is a partial image segmented into the size of $N{\times}N$. Each partial image retains the information of the whole object. This method generates an assembled image for a row of the segmented and frequency-transformed partial images, which is the basis of the coding process. That is, a motion estimation and compensation technique of MPEG is applif:d to the reconstructed images from the assembled images with the disparities found during generation of assembled image and the original partial images. Therefore the compressed results are the disparity of eachpartial image to form the assembled image for the corresponding row, assembled image, and the motion vectors and the compensated image for each partial image. The experimental results with the implemented algorithm showed that the proposed method has NC (Normal Correlation) values about 4% higher than the previous method, by which ours has better compression efficiency. Consequently, the Proposed method is expected to be used effectively in the application areas to transmit the digital hologram data. can be identified in comparison with the previous researches and commercial IPs.

• Journal of Life Science
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• v.25 no.12
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• pp.1445-1449
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• 2015

The pinewood nematode Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae) poses a serious threat to pine forests in Europe and East Asia, leading to a debilitating pine wilt disease. Infected pine trees in Korea are generally fumigated or crushed to small wood chips after felling. Although pine wilt disease often recurs in pest management sites, there are no adequate means to monitor the effectiveness of pest control measures in those sites. Recently, a male-produced aggregation pheromone, 2-(undecyloxy)ethanol, was shown to be useful for attracting several Monochamus species, which are vectors for the pinewood nematodes. In this study, we investigated the abilities of 2-(undecyloxy)ethanol at three different doses (175, 350, and 700 mg), as well as host plant volatiles (α-pinene and ethanol), to attract M. alternatus (Coleoptera: Cerambycidae) at a pine forest in Pohang, Korea where infected pine trees had been cut down and fumigated. Twenty-seven M. alternatus were captured in cross-vane panel traps made of polyethylene terephthalate bottles and acrylic sheets. The results indicate that a high dose of 2-(undecyloxy)ethanol (700 mg per trap) is the most effective for attracting M. alternatus. The aggregation pheromone could be used to monitor the effectiveness of pest control measures as well as M. alternatus populations.

• Journal of Life Science
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• v.22 no.8
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• pp.1018-1023
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• 2012

Snail is a zinc finger transcription factor that induces epithelial-to-mesenchymal transition (EMT), which promotes tumor invasion and metastasis by repressing E-cadherin expression. In addition, Snail restricts the cellular apoptotic response to apoptotic stimuli or survival factor withdrawal; however, its molecular mechanism remains largely unknown. In this study, we have investigated the mechanism underlying Snail-mediated chemoresistance to 5-fluorouracil (5-FU), one of the most widely used anti-cancer drugs. When Snail was overexpressed by doxycycline (DOX) in MCF-7 #5 cells, it inhibited 5-FU-induced apoptotic cell death and switched the cell death mode to necrosis. Snail expression, either by DOX treatment in MCF-7 #5 cells or by the transfection of Snail expression vectors pCR3.1-Snail-Flg, phosphorylation-resistant pCR3.1-S104, and 107A Snail-Flg in MCF-7 cells specifically induced PTEN down-regulation/inactivation and Akt/PKB activation, without affecting ERK1/2 activity. In addition, Snail prominently suppressed 5-FU-induced increases in p53 levels. These findings demonstrate that Snail switches 5-FU-induced apoptosis to necrosis through the activation of Akt/PKB and the down-regulation of p53 levels.

• The Mathematical Education
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• v.57 no.3
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• pp.197-213
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• 2018

Descriptive problems can be used to grow student's ability of thinking logically and creatively, because it shows if the students had a reasonable way of thinking. Rate of descriptive problems is increasing in middle and high school exams. However, students in middle and high schools are generally used to answering multiple-choice or short-answer questions rather than describing the solving process. The purpose of this paper is to gain a theoretic ground to increase the rate of descriptive problems. In this study, students were to solve some multiple-choice problems, and after a few weeks, to solve the problems of same contents in the form of descriptive problems which requires the students to write the solving process. The difference of the scores were measured for each problems to each students, and students were asked what they think the reason for rise or fall of the score is. The result is as follows: First, average scores of 7 of 8 problems used in this study had fallen when it was in descriptive form, and for 5 of them in the rate of 11.2%~16.8%. Second, the main reason of falling is that the students have actual troubles of describing the solving process. Third, in the case of rising, the main reason was that partial scores were given in the descriptive problems. Last, there seems a possibility gender difference in the reason of falling. From these results, followings are suggested to advance the learning, teaching and evaluation in mathematics education: First, it has to be emphasized enough to describe the solving process when solving a problem. Second, increasing the rate of descriptive problems can be supported as a way to advance the evaluation. Third, descriptive problems have to be easier to solve than multiple-choice ones and it is convenient for the students to describe the solving process. Last, multiple-choice problems have to be carefully reviewed that the possibility of students' choosing incorrect answer with a small mistake is minimal.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.16 no.1
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• pp.27-40
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• 1998

This paper addresses the suitability of GPS positioning technology to monitoring deformation and movement of structures. The first part of the study is an empirical quantitative study of the repeatability of GPS observations and the second part is a performance evaluation of kinematic GPS, which requires only a few minutes per a point, for monitoring deformation of an engineering structure. On the test network for monitoring of a earth am, four observations have been conducted repeatedly on different seasons and water levels. The reference network was observed in static mode, and monitoring points were observed respectively in rapid-static mode as well as in kinematic mode in each epoch and then the results were compared with those obtained by conventional surveying techniques. The repeatability of baseline vectors to better than average 7 mm in three dimensions was achieved in base line observations between reference points and also the unclosure of reference networks showed the range of 4 ppm to 27 ppm. Compared with conventional surveying techniques, the kinematic approach showed the differences of 3∼4 m in slope distances which were observed from reference points to monitoring points, and showed the differences of 4∼8 m in height. It was ascertained that the kinematic GPS technology provides an efficient alternative to deformation monitoring by conventional means which are capable of detecting movements in the order of 5 mm.

• Parasites, Hosts and Diseases
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• v.35 no.3
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• pp.171-180
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• 1997

Studies on geographical distributions and relative population densities of the vector mites of tsutsugamushi disease were carried out in October 1996 at 12 locations of the mid south inland of the Korean peninsula, where chigger mites have been never studied. Of 177 field rodents and insectivores collected. 154 (87.0%) were Apodemus ngrarius. Total 25,707 chigger mites were collected and 14 species were identified. of which Leptotrombidium pnllidum was predominant (79.8%) and L. palpate the next (8.9%) . L. pallidum, the vector species, was widely distributed in all study areas, showing the highest density at Cho-o 2-dong, Sangju-si (chigger index 201.8), and the lowest at Tanwol-dong. Chungiu-si (chigger index 40.7) . The other vector species, L. scutellare was found only at the southern part of the study area such as Yobae and Mipyong, Kumrung gun and Vnsu , Kimchon-si. The northernmost areas of the L. scutellcre distribution were coincided with the areas where actual mean air temperature is above 10.0%. Among 157 A. ograrius sera tested, 48.3% was Karp, 1.7% william and 3.3% Kuroki. The rest of the sera were not able to determine the sexto-type because of the cross antigen-antibody reactions among the tested sero-types.

• Korean Journal of Veterinary Research
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• v.52 no.3
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• pp.183-191
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• 2012

The maintenance of peripheral immune tolerance and prevention of chronic inflammation and autoimmune disease require $CD4^{+}CD25^{+}$ T cells (regulatory T cells). The transcription factor Foxp3 is essential for the development of functional, regulatory T cells, which plays a prominent role in self-tolerance. Retroviral vectors can confer high level of gene transfer and transgene expression in a variety of cell types. Here we observed that following retroviral vector-mediated gene transfer of Foxp3, transductional Foxp3 expression was increased in the liver, lung, brain, heart, muscle, spinal cord, kidney and spleen. One day after vector administration, high levels of transgene and gene expression were observed in liver and lung. At 2 days after injection, transductional Foxp3 expression level was increased in brain, heart, muscle and spinal cord, but kidney and spleen exhibited a consistent low level. This finding was inconsistent with the increase in both $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell frequencies observed in peripheral immune cells by fluorescence-activated cell-sorting (FACS) analysis. Retroviral vector-mediated gene transfer of Foxp3 did not lead to increased numbers of $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell. These results demonstrate the level and duration of transductional Foxp3 gene expression in various tissues. A better understanding of Foxp3 regulation can be useful in dissecting the cause of regulatory T cells dysfunction in several autoimmune diseases and raise the possibility of enhancing suppressive functions of regulatory T cells for therapeutic purposes.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.27 no.5
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• pp.555-563
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• 2009

When a mobile mapping system or a robot is equipped with only a GPS (Global Positioning System) and multiple stereo camera system, a transformation from a local camera coordinate system to GPS coordinate system is required to link camera poses and 3D data by V-SLAM (Vision based Simultaneous Localization And Mapping) to GIS data or remove the accumulation error of those camera poses. In order to satisfy the requirements, this paper proposed a novel method that calculates a camera rotation in the GPS coordinate system using the three pairs of camera positions by GPS and V-SLAM, respectively. The propose method is composed of four simple steps; 1) calculate a quaternion for two plane's normal vectors based on each three camera positions to be parallel, 2) transfer the three camera positions by V-SLAM with the calculated quaternion 3) calculate an additional quaternion for mapping the second or third point among the transferred positions to a camera position by GPS, and 4) determine a final quaternion by multiplying the two quaternions. The final quaternion can directly transfer from a local camera coordinate system to the GPS coordinate system. Additionally, an update of the 3D data of captured objects based on view angles from the object to cameras is proposed. This paper demonstrated the proposed method through a simulation and an experiment.