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http://dx.doi.org/10.5010/JPB.2002.29.4.235

Primer for the Potato Specific Internal Control DNA and Screening Method for the Genetically Modified Potatoes by Competitive Duplex-PCR  

Seo, Hyo-Won (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA)
Yi, Jung-Yoon (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA)
Cho, Hyun-Mook (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA)
Kim, Sung-Yeul (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA)
Publication Information
Journal of Plant Biotechnology / v.29, no.4, 2002 , pp. 235-240 More about this Journal
Abstract
We report the new method for the screening of genetically modified potato by competitive duplex-PCR using the potato specific single oligomer primer for the internal control and CaMV 35S promoter or NOS terminator specific primers. The single oligomer primer (rAGU4A) amplify the potato specific internal control band from the homozygous potato genomic DNA in the RAPD profiles of all analyzed potato varieties. The 530 bp internal control DNA was amplified independently to CaMV 35S promoter or NOS terminator DNA and identified as repetitive or microsatellite DNA of potato (AF541972). With this new technique, the transgenic potatoes which were transformed with vectors contained the different foreign genes are analyzed. In case of the commercialized transgenic potato varieties, 'Hew Leafs', those two genetic factors are used for promoter and terminator respectively So, this new PCR technique should be a promising method of cost effective and accurate screening for the commercialized GM potatoes on market.
Keywords
Duplex-PCR; GMO screening; internal control; potato;
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1 Anonymous (2002) http://www.bats.ch/about/projekte.htm
2 Bonierbale MW, Plaisted RL, Tanksley SD (1991) Application of restriction fragment length polymorphisms and genetic mapping to potato breeding. In : International Potato Center, Molecular Methods for Potato Improvement. Report of the Planning Conference on 'Application of Molecular Techniques to Potato Germplasm Enhancement'. CIP, Peru, PP. 149-157
3 Brunnert HJ, Spener F, Borchers T (2001) PCR-ELISA for the CaMV-35S promoter as a screening method for genetically modified Roundup Ready soybeans. Eur Food Res Technol 213: 366-371   DOI   ScienceOn
4 Elliott AR, Campbe JA, Dugdale B, Brettel RSI, Grof CPL (1999) Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells. PIant Cell Rep 18:707-714   DOI   ScienceOn
5 Hall TC (2002) Current status of GMO: development and perspective. In : International symposium on agrobiotechnology for 21st century, RDA, Suwon Korea, PP.79-102
6 $H\ddot{u}bner$ P, Studer E, $L\ddot{u}thy$ J (1999) Quantitative competitive PCR for the detection of genetically modified organism in food. Food Control 10:353-358   DOI   ScienceOn
7 Grunst T, Kephart D (2001) Wizard Magnetic DNA purification system for food: Part II. Semi-automated DNA isolation and analysis of GMO foods. Pomega Note 76:19-22
8 James C (2002) Global hectarage of GM crops in 2001. Crop Biotech Brief Vol.II No. 1
9 Kang HW, Park DS, Go SJ, Eun MY (2002) Fingerprinting of diverse genomes using PCR with Universal Rice Primers generated from prpetitive sequence of Korean Weed Rice. Mol Cells 13:281-287
10 Lipp M, Brodmann P, Puetsch K, Pauwels J, Anklam E (1999). IUPAC collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder. J AOAC Int 82: 923-928
11 $Mannerl\ddot{o}ef$ M, Tenning P (1996) Screening of transgenic plant by multiplex PCR. Plant Mol Biol Rep 15:38-45   DOI   ScienceOn
12 Meyer R (1999) Development and application of DNA analytical methods for the detection of GMOs in food. Food Control 10: 391-399   DOI   ScienceOn
13 Teresa MF, Julapark C, Tanksley SD (1995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol Biol Rep 13: 207-209   DOI   ScienceOn
14 Seo HW, Yi JY, Cho HM, Cho JH, Park YE (2001) Development of maturity controllable transgenic potato with GA 3beta-hydroxylase. In : Annual Research Report of National Highland Agiicultural Experiment Station, RDA, Korea. pp. 342-345
15 Spoth B, Strauss E (1999) Screening for genetically modified organisms in food using Promegas Wizard Resin. Promega Notes 73:23-25
16 Tao Z, Cai XF, Yang SL, Gong Y (2001) Detection of exogenous genes in genetically modified with multiplex polymerase chain reaction. Plant Mol Biol Rep 19:289-298   DOI
17 Tozzini AC, Martinez MC, Lucca, MF, Rovere CV, Distefano AJ, del Vas M, Hopp HE (2000) Semi-quantitative detection of genetically modified grains based on CaMV 35S promoter amplification. Electric Journal of Biotechnology 3:149-153
18 Vickers JE, Graham GC, Henry RJ (1996) A protocol for the screening of putatively transformed plant for bar, the selectable marker gene, using the polymerase chain reaction. Plant Mol Biol Rep 14:363-368   DOI   ScienceOn
19 Wurz, A, Bluth A, Zeltz P, Pfeifer C, Willmund R (1999) Quantitative analysis of genetically modified organism (GMO) on processed food by PCR-based methods. Food Control 10:385-389   DOI   ScienceOn
20 Yi JY, Lee SW, Park KW (1998) Introduction of Shiva gene into tobacco and potato using tissue-specific tomato PAL promoter. Korean J. PIant Tissue Culture 25:109-113
21 Chiueh LC, Chen YL, Daniel TCS (2002) Study on the detection method of six varieties of genetically modified maize and processed foods. J Food Drug Anal 10:25-33
22 Seo HW (2001) Genetic engineering of virus resistant potatoes and development of nucleic acid diagnostic methods using coat protein gene of Potato leafroll virus. PhD thesis, Konkuk University, Seoul. pp. 51-81