• Archives of Plastic Surgery
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• 제48권1호
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• pp.84-90
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• 2021

Vascularized bone grafts (VBGs) are widely employed to reconstruct upper extremity bone defects. Conventional bone grafting is generally used to treat defects smaller than 5-6 cm, when tissue vascularization is adequate and there is no infection risk. Vascularized fibular grafts (VFGs) are mainly used in the humerus, radius or ulna in cases of persistent non-union where traditional bone grafting has failed or for bone defects larger than 6 cm. Furthermore, VFGs are considered to be the standard treatment for large bone defects located in the radius, ulna and humerus and enable the reconstruction of soft-tissue loss, as VFGs can be harvested as osteocutaneous flaps. VBGs enable one-stage surgical reconstruction and are highly infection-resistant because of their autonomous vascularization. A vascularized medial femoral condyle (VFMC) free flap can be used to treat small defects and non-unions in the upper extremity. Relative contraindications to these procedures are diabetes, immunosuppression, chronic infections, alcohol, tobacco, drug abuse and obesity. The aim of our study was to illustrate the use of VFGs to treat large post-traumatic bone defects and osteomyelitis located in the upper extremity. Moreover, the use of VFMC autografts is presented.

• Journal of Trauma and Injury
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• 제37권2호
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• pp.161-165
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• 2024

Femoral head fractures with associated hip dislocations substantially impact the functional prognosis of the hip joint and present a surgical challenge. The surgeon must select a safe approach that enables osteosynthesis of the fracture while also preserving the vascularization of the femoral head. The optimal surgical approach for these injuries remains a topic of debate. A 44-year-old woman was involved in a road traffic accident, which resulted in a posterior iliac dislocation of the hip associated with a Pipkin type II fracture of the femoral head. Given the size of the detached fragment and the risk of incarceration preventing reduction, we opted against attempting external orthopedic reduction maneuvers. Instead, we chose to perform open reduction and internal fixation using the Watson-Jones anterolateral approach. This involved navigating between the retracted tensor fascia lata muscle, positioned medially, and the gluteus medius and minimus muscles, situated laterally. During radiological and clinical follow-up visits extending to postoperative month 15, the patient showed no signs of avascular necrosis of the femoral head, progression toward coxarthrosis, or heterotopic ossification. The Watson-Jones anterolateral approach is a straightforward intermuscular and internervous surgical procedure. This method provides excellent exposure of the femoral head, preserves its primary vascularization, allows for anterior dislocation, and facilitates the anatomical reduction and fixation of the fracture.

• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2507-2511
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• 2016

Purpose: To study the diagnostic accuracies of serum human epididymis protein 4 (HE-4) levels, virtual organ computer-aided analysis (VOCAL) parameters and endometrial volume in endometrial cancer cases. Materials and Methods: One hundred and seven patients (37 with endometrial cancer and 70 with benign endometrial pathology) were included in this study. VOCAL parameters and serum HE-4 levels were compared between the groups. Results: Area under the curve (AUC) values were 0.702, 0.658, 0.706 for vascularization index (VI), the flow index (FI) and the vascularization flow index (VFI), respectively. A cut off value of 0.568 for VI demonstrated 70% sensitivity, 72% specificity, 56% positive predictive value (PPV) and a81% negative predictive value (NPV). A cut off value of 25.8 for showed a senitivith of 70% and a specificity of 58% with aPPV of 46% and NPV of 78%, and with a cut off value of 0.12 for VFI 70%, 69%, 54% and 81%, respectively. The area under the curve for HE-4 was 0.814. A cut off value of 458 pmol/L was predictive of malignancy with 86% sensitivity and 63% specificity. Conclusions: VOCAL parameters and serum HE-4 levels were statistically significantly higher in the endometrial cancer patients. Serum HE-4 levels provided a greater sensitivity compared to power doppler angiography for predicting malignancy or benign endometrial pathology.

• Archives of Plastic Surgery
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• 제35권2호
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• pp.121-126
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• 2008

Purpose: Poly-N-acetyl glucosamine(PGlcNAc) nanofiber-based materials, produced by a marine microalga, have been characterized as effective hemostatic and angiogenic agents. The similarity between PGlcNAc patch and the natural extracellular matrix allows it to support new healthy tissue growth in an injured area and to encourage fluid absorption. In this study, we hypothesized that a poly-N-acetyl glucosamine fiber patch(PGlcNAc patch) may enhance wound healing in the db/db mouse. Methods: PGlcNAc patches were applied on one square centimeter, full-thickness, skin wounds in the db/db mouse model. Wounds(n=15 per group) were dressed with a PGlcNAc nanofiber patch for 1 hour(1 h), 24 hours(24 h) or left untreated(NT). After the application time, patches were removed and wounds were allowed to heal spontaneously. The rate of wound closure was evaluated by digital analysis of unclosed wound area in course of time. At day 10, wounds(n=7 per group) were harvested and quantified with immunohistochemical markers of proliferation(Ki-67) and vascularization (platelet endothelial cell adhesion molecule, PECAM-1). Results: Wounds dressed with PGlcNAc patches for 1 hour closed faster than control wounds, reaching 90% closure in 16.6 days, nine days faster than untreated wounds. Granulation tissue showed higher levels of proliferation and vascularization following 1 h treatment than the 24 h and NT groups. In addition to its hemostatic properties, the PGlcNAc material also appears to accelerate wound closure in healing-impaired genetically diabetic mice. Conclusion: This material, with its combination of hemostatic and wound healing properties, has the potential to be effective agent for the treatment of complicated wounds.

• 한국어류학회지
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• 제36권1호
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• pp.40-47
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• 2024

본 연구에서는 드렁허리 Monopterus albus 후각기관의 형태 및 조직학적 특성을 실체, 광학 및 주사전자현미경하 관찰을 통하여 조사하였다. 후각기관의 외부 형태는 피부와 평행한 폐쇄형 전비공과 후비공을 나타냈다. 내부 구조는 파이프형 비강과 뒤쪽의 위아래의 두 개 비낭으로 구성되었다. 비강의 내벽은 위중층상피층의 감각상피와 중층편평상피층의 비감각상피, 미확인기관으로 이루어졌다. 감각상피는 후감각뉴런, 지지세포, 기저세포, 림프구로 구성되며, 비감각상피는 중층상피세포와 산성의 점액다당류를 보유하는 점액세포를 보유했다. 특히, 감각상피층 내 모세혈관과 풍부한 진피에서의 혈관화는 논, 농수로, 연못, 늪과 같은 진흙 하상의 정체된 수환경에 적응 된 후각기관 내 부가적인 호흡기능의 증거이며 이는 이전에 원구류 이상의 어류 분류군에서 확인되지 않은 새로운 결과로 확인된다.

• International Journal of Stem Cells
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• 제16권1호
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• pp.1-15
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• 2023

Liver organoids have gained much attention in recent years for their potential applications to liver disease modeling and pharmacologic drug screening. Liver organoids produced in vitro reflect some aspects of the in vivo physiological and pathological conditions of the liver. However, the generation of liver organoids with perfusable luminal vasculature remains a major challenge, hindering precise and effective modeling of liver diseases. Furthermore, vascularization is required for large organoids or assembloids to closely mimic the complexity of tissue architecture without cell death in the core region. A few studies have successfully generated liver organoids with endothelial cell networks, but most of these vascular networks produced luminal structures after being transplanted into tissues of host animals. Therefore, formation of luminal vasculature is an unmet need to overcome the limitation of liver organoids as an in vitro model investigating different acute and chronic liver diseases. Here, we provide an overview of the unique features of hepatic vasculature under pathophysiological conditions and summarize the biochemical and biophysical cues that drive vasculogenesis and angiogenesis in vitro. We also highlight recent progress in generating vascularized liver organoids in vitro and discuss potential strategies that may enable the generation of perfusable luminal vasculature in liver organoids.

• 대한핵의학회지
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• 제33권6호
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• pp.484-492
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• 1999

목적: 안구제거술 후 삽입한 hydroxyapatite 안구보충물 내로의 혈관 신생 여부의 평가에 $^{99m}Tc$-MDP 골신티그라피의 임상적 유용성에 대해 알아보고자 하였다. 대상 및 방법: 안구적출술이나 안구내용물 제거술의 방법을 이용하여 안구 제거 후 hydroxyapatite 보충물을 삽입한 24명의 환자를 대상으로 하였다(여자 7명, 남자 17명, 연령 분포 $12{\sim}78$세, 평균 연령 36세, 안구적출술 12명, 안구내용물 제거술12명) Hydroxyapatite 보충물 삽입 후 $3{\sim}33$주($3{\sim}10$주 사이에 4명, $11{\sim}20$주 사이에 10명, $21{\sim}33$주 사이에 10명) 사이에 $^{99m}Tc$-MDP 골신티그라피를 시행하였다. 방사능 섭취 정도는 육안적으로 안구보충물과 비교(nasal bridge) 및 반대측 안구와 비교하여 안구 보충물이 비교보다 강할 경우 등급 4, 같을 경우 등급 3, 비교와 반대측 안구 사이일 경우 등급 2, 그 이하일 경우 등급 1로 하였다. 또한 골신티그라피 정면 영상에서 관심영역을 안구 보충물과 반대측 안구에 같은 크기로 설정하여 방사능 계수 비(H/N ratio)를 구하였다. 검사 시기와 방사능 섭취의 등급 분류, 방사능 계수 비와 안구 고정술 후 성공여부와의 관계를 분석하였다. 결과: 등급 2 이상, 방사능 계수 비가 1.56 이상인 환자 중 추적 관찰할 수 없었던 2명을 제외한 19명 모두에서 천공 시 출혈을 확인하였고, 의안과 연결하여 안구 고정술을 시행하였다. 방사능 섭취 정도를 육안적 분류 방법과 정량적 측정 방법에 의한 결과는 두 방법 모두 통계적으로 유의하였고 검사 시기와 방사능 계수 비 사이에 각각 유의한 상관관계가 있었으나 수술 방법에 따른 차이는 보이지 않았다. 결론: $^{99m}Tc$-MDP 골신티그라피의 방사능 섭취 등급 분류와 방사능 계수 비는 안구고정술의 시기 결정에 도움을 주었다. 안구적출술과 안구내용물제거술의 수술 방법 차이에 관계없이 안구고정술을 위한 첫 검사 시기는 안구보충물삽입 후 $11{\sim}20$주 사이가 좋을 것으로 생각되며, 등급 2, 방사능 계수 비 1.56 이상인 경우에는 안구 고정술을 시행하여 좋은 결과를 기대할 수 있을 것으로 생각된다.

• PNF and Movement
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• 제6권2호
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• pp.51-57
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• 2008

Purpose : Mechanical stress activates signaling cascades and leading to a specific response of a network of signaling pathways. The purpose of this study is to review the effect of mechanical stress-induced adaptation in skeletal muscle involves a biological mechanisms. Methods : This is literature study with Pubmed, Medline and books. Results : Skeletal muscle tissue demonstrates a malleability and may adjust its metabilic response, vascularization and neuromuscular characteristic makeup in response to alteration in functional demands. The adaptation in skeletal muscle involoves a multitude of signalling mechanisms related with insuline-like growth factor, vascular endothelial growth factor, neurotrophins. Conclusions : The identification of the basic relationships underlying the malleability of skeletal muscle tissue is likely to be of relevance for our understanding with PNF technique.

• Asian Pacific Journal of Cancer Prevention
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• 제16권5호
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• pp.1677-1682
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• 2015

Cancer progression is attained by uncontrolled cell division and metastasis. Increase in tumor size triggers different vascular channel formation to address cell nutritional demands. These channels are responsible for transferring of nutrients and gaseous to the cancer cells. Cancer vascularization is regulated by numerous factors including vascular endothelial growth factors (VEGFs). These factors play an important role during embryonic development. Members included in this group are VEGFA, VEGFB, VEGFC, PIGF and VEGFD which markedly influence cellular growth and apoptosis. Being freely diffusible these proteins act in both autocrine and paracrine fashions. In this review, genetic characterization these molecules and their putative role in cancer staging has been elaborated. Prognostic significance of these molecules along with different stages of cancer has also been summarized. Brief outline of ongoing efforts to target hot spot target sites against these VEGFs and their cognate limitations for therapeutic implications are also highlighted.

• 대한추나의학회지
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• 제4권1호
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• pp.55-65
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• 2003

The purpose of this article was to contribute to the knowledge of physiological and pathological changes of aging skeletal muscles, and of therapic method. By aging there were changes of distribution of muscle fibers, the loss of muscle mass, the loss of the number of muscle fibers, the loss of glycolysis capacity, the decrease of the oxidative enzymes and muscle vascularization in the skeletal muscles. And as a pathological change, the exhaustive maximal exercise increased oxidative stress that led to oxidative damage which were shown to be implicated in promoting aging. The property of intensity and duration exercise is important not only in keeping human health and physical fitness from oxidative stress, but also for the maintenance of well-being and quality of life.